Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice

Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice

Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis which results in cellular toxicity. The salt overly sensitive 1 (SOS1) gene is a critical component of salt tolerance in many species and encodes a plasma membrane Na+/H+ antiporter that plays an imp...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plant tissue culture & biotechnology 2014-01, Vol.23 (2), p.263-273
Hauptverfasser: Razzaque, Samsad, Elias, Sabrina M., Biswas, Sudip, Haque, Taslima, Seraj, Zeba I.
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 273
container_issue 2
container_start_page 263
container_title Plant tissue culture & biotechnology
container_volume 23
creator Razzaque, Samsad
Elias, Sabrina M.
Biswas, Sudip
Haque, Taslima
Seraj, Zeba I.
description Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis which results in cellular toxicity. The salt overly sensitive 1 (SOS1) gene is a critical component of salt tolerance in many species and encodes a plasma membrane Na+/H+ antiporter that plays an important role in germination and growth in saline environments. In the current study, the coding sequence of SOS1 gene (3447 bp) was amplified from a Bangladeshi rice landrace, Pokkali, by applying the fusion PCR strategy. SOS1 PCR amplicons were firstly cloned into pENTR/D-TOPO and then recombined with the binary vector, pH7WG2, through LR reaction. Positive colonies were validated by PCR, restriction digestion and sequencing. Finally, the constructed vector was transformed into Agrobacterium tumeficiens, LBA4404 strain, to initiate Agrobacterium-mediated transformation with the provision to transfer the cloned SOS1 into farmer popular rice varieties. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17527 Plant Tissue Cult. & Biotech. 23(2): 263-273, 2013  (December)
doi_str_mv 10.3329/ptcb.v23i2.17527
format Article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_3329_ptcb_v23i2_17527</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_3329_ptcb_v23i2_17527</sourcerecordid><originalsourceid>FETCH-LOGICAL-c158t-1789e6a13b7af28bb199c2422616cf27170aa6cfb50eacb9a6f50983bb847d433</originalsourceid><addsrcrecordid>eNotkMFLwzAchYMoOObuHvMPdMsvaZvkOIY6YTJxerUkaTIDa1KSOtx_bzc9vY93eA8-hO6BzBmjctEPRs-PlHk6B15RfoUmIEAUgpfV9YV5wTiFWzTL2WtCGEApq3KCPleHGHzY4-jw8GXx60HlTuEX2-mkgsW72PrvbrE-tSnubcDLMPg-psEmvNvuALuYsB8y3h7Hxv70yY4HMWAf8Js39g7dOHXIdvafU_Tx-PC-Wheb7dPzarkpDFRiKIALaWsFTHPlqNAapDS0pLSG2jjKgROlRtIVscpoqWpXESmY1qLkbcnYFJG_XZNizsm6pk--U-nUAGnOipqzouaiqLkoYr8Talub</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice</title><source>Bangladesh Journals Online</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Razzaque, Samsad ; Elias, Sabrina M. ; Biswas, Sudip ; Haque, Taslima ; Seraj, Zeba I.</creator><creatorcontrib>Razzaque, Samsad ; Elias, Sabrina M. ; Biswas, Sudip ; Haque, Taslima ; Seraj, Zeba I.</creatorcontrib><description>Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis which results in cellular toxicity. The salt overly sensitive 1 (SOS1) gene is a critical component of salt tolerance in many species and encodes a plasma membrane Na+/H+ antiporter that plays an important role in germination and growth in saline environments. In the current study, the coding sequence of SOS1 gene (3447 bp) was amplified from a Bangladeshi rice landrace, Pokkali, by applying the fusion PCR strategy. SOS1 PCR amplicons were firstly cloned into pENTR/D-TOPO and then recombined with the binary vector, pH7WG2, through LR reaction. Positive colonies were validated by PCR, restriction digestion and sequencing. Finally, the constructed vector was transformed into Agrobacterium tumeficiens, LBA4404 strain, to initiate Agrobacterium-mediated transformation with the provision to transfer the cloned SOS1 into farmer popular rice varieties. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17527 Plant Tissue Cult. &amp; Biotech. 23(2): 263-273, 2013  (December)</description><identifier>ISSN: 1817-3721</identifier><identifier>EISSN: 1818-8745</identifier><identifier>DOI: 10.3329/ptcb.v23i2.17527</identifier><language>eng</language><ispartof>Plant tissue culture &amp; biotechnology, 2014-01, Vol.23 (2), p.263-273</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c158t-1789e6a13b7af28bb199c2422616cf27170aa6cfb50eacb9a6f50983bb847d433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4125,27924,27925</link.rule.ids></links><search><creatorcontrib>Razzaque, Samsad</creatorcontrib><creatorcontrib>Elias, Sabrina M.</creatorcontrib><creatorcontrib>Biswas, Sudip</creatorcontrib><creatorcontrib>Haque, Taslima</creatorcontrib><creatorcontrib>Seraj, Zeba I.</creatorcontrib><title>Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice</title><title>Plant tissue culture &amp; biotechnology</title><description>Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis which results in cellular toxicity. The salt overly sensitive 1 (SOS1) gene is a critical component of salt tolerance in many species and encodes a plasma membrane Na+/H+ antiporter that plays an important role in germination and growth in saline environments. In the current study, the coding sequence of SOS1 gene (3447 bp) was amplified from a Bangladeshi rice landrace, Pokkali, by applying the fusion PCR strategy. SOS1 PCR amplicons were firstly cloned into pENTR/D-TOPO and then recombined with the binary vector, pH7WG2, through LR reaction. Positive colonies were validated by PCR, restriction digestion and sequencing. Finally, the constructed vector was transformed into Agrobacterium tumeficiens, LBA4404 strain, to initiate Agrobacterium-mediated transformation with the provision to transfer the cloned SOS1 into farmer popular rice varieties. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17527 Plant Tissue Cult. &amp; Biotech. 23(2): 263-273, 2013  (December)</description><issn>1817-3721</issn><issn>1818-8745</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNotkMFLwzAchYMoOObuHvMPdMsvaZvkOIY6YTJxerUkaTIDa1KSOtx_bzc9vY93eA8-hO6BzBmjctEPRs-PlHk6B15RfoUmIEAUgpfV9YV5wTiFWzTL2WtCGEApq3KCPleHGHzY4-jw8GXx60HlTuEX2-mkgsW72PrvbrE-tSnubcDLMPg-psEmvNvuALuYsB8y3h7Hxv70yY4HMWAf8Js39g7dOHXIdvafU_Tx-PC-Wheb7dPzarkpDFRiKIALaWsFTHPlqNAapDS0pLSG2jjKgROlRtIVscpoqWpXESmY1qLkbcnYFJG_XZNizsm6pk--U-nUAGnOipqzouaiqLkoYr8Talub</recordid><startdate>20140103</startdate><enddate>20140103</enddate><creator>Razzaque, Samsad</creator><creator>Elias, Sabrina M.</creator><creator>Biswas, Sudip</creator><creator>Haque, Taslima</creator><creator>Seraj, Zeba I.</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20140103</creationdate><title>Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice</title><author>Razzaque, Samsad ; Elias, Sabrina M. ; Biswas, Sudip ; Haque, Taslima ; Seraj, Zeba I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c158t-1789e6a13b7af28bb199c2422616cf27170aa6cfb50eacb9a6f50983bb847d433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Razzaque, Samsad</creatorcontrib><creatorcontrib>Elias, Sabrina M.</creatorcontrib><creatorcontrib>Biswas, Sudip</creatorcontrib><creatorcontrib>Haque, Taslima</creatorcontrib><creatorcontrib>Seraj, Zeba I.</creatorcontrib><collection>CrossRef</collection><jtitle>Plant tissue culture &amp; biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Razzaque, Samsad</au><au>Elias, Sabrina M.</au><au>Biswas, Sudip</au><au>Haque, Taslima</au><au>Seraj, Zeba I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice</atitle><jtitle>Plant tissue culture &amp; biotechnology</jtitle><date>2014-01-03</date><risdate>2014</risdate><volume>23</volume><issue>2</issue><spage>263</spage><epage>273</epage><pages>263-273</pages><issn>1817-3721</issn><eissn>1818-8745</eissn><abstract>Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis which results in cellular toxicity. The salt overly sensitive 1 (SOS1) gene is a critical component of salt tolerance in many species and encodes a plasma membrane Na+/H+ antiporter that plays an important role in germination and growth in saline environments. In the current study, the coding sequence of SOS1 gene (3447 bp) was amplified from a Bangladeshi rice landrace, Pokkali, by applying the fusion PCR strategy. SOS1 PCR amplicons were firstly cloned into pENTR/D-TOPO and then recombined with the binary vector, pH7WG2, through LR reaction. Positive colonies were validated by PCR, restriction digestion and sequencing. Finally, the constructed vector was transformed into Agrobacterium tumeficiens, LBA4404 strain, to initiate Agrobacterium-mediated transformation with the provision to transfer the cloned SOS1 into farmer popular rice varieties. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17527 Plant Tissue Cult. &amp; Biotech. 23(2): 263-273, 2013  (December)</abstract><doi>10.3329/ptcb.v23i2.17527</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1817-3721
ispartof Plant tissue culture & biotechnology, 2014-01, Vol.23 (2), p.263-273
issn 1817-3721
1818-8745
language eng
recordid cdi_crossref_primary_10_3329_ptcb_v23i2_17527
source Bangladesh Journals Online; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
title Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T17%3A15%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20of%20the%20Plasma%20Membrane%20Sodium/Hydrogen%20Antiporter%20SOS1%20for%20its%20Over%20expression%20in%20Rice&rft.jtitle=Plant%20tissue%20culture%20&%20biotechnology&rft.au=Razzaque,%20Samsad&rft.date=2014-01-03&rft.volume=23&rft.issue=2&rft.spage=263&rft.epage=273&rft.pages=263-273&rft.issn=1817-3721&rft.eissn=1818-8745&rft_id=info:doi/10.3329/ptcb.v23i2.17527&rft_dat=%3Ccrossref%3E10_3329_ptcb_v23i2_17527%3C/crossref%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true