Detection of snow mold fungi in wheat plants by means of polyacrylamide gel electrophoresis of ribosomal proteins
Ribosomes were isolated from four snow mold fungi (Pythium paddicum, P. iwayamai, Typhula incarnata and Fusarium nivale) and wheat seedlings, and their proteins were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). No difference was found in electrophoretic patterns between P. paddicum...
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| Veröffentlicht in: | Japanese Journal of Phytopathology 1987/12/25, Vol.53(5), pp.591-597 |
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| container_title | Japanese Journal of Phytopathology |
| container_volume | 53 |
| creator | Takenaka, S. (Hokuriku National Agricultural Experiment Station, Joetsu, Niigata (Japan)) Yoshino, R |
| description | Ribosomes were isolated from four snow mold fungi (Pythium paddicum, P. iwayamai, Typhula incarnata and Fusarium nivale) and wheat seedlings, and their proteins were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). No difference was found in electrophoretic patterns between P. paddicum and P. iwayamai. However, there were several differences among two Pythium species, T. incarnata, F. nivale and wheat. The ribosomes were isolated also from wheat seedlings infected with P. paddicum, T. incarnata and F. nivale individually, and their proteins were analyzed by SDS-PAGE. The distinctive fungal ribosomal protein bands were detected in all patterns obtained from the infected wheat plants. The possibility of the ribosomal protein-based assay to detect snow mold fungi in wheat plants is suggested. |
| doi_str_mv | 10.3186/jjphytopath.53.591 |
| format | Article |
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(Hokuriku National Agricultural Experiment Station, Joetsu, Niigata (Japan)) ; Yoshino, R</creator><creatorcontrib>Takenaka, S. (Hokuriku National Agricultural Experiment Station, Joetsu, Niigata (Japan)) ; Yoshino, R</creatorcontrib><description>Ribosomes were isolated from four snow mold fungi (Pythium paddicum, P. iwayamai, Typhula incarnata and Fusarium nivale) and wheat seedlings, and their proteins were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). No difference was found in electrophoretic patterns between P. paddicum and P. iwayamai. However, there were several differences among two Pythium species, T. incarnata, F. nivale and wheat. The ribosomes were isolated also from wheat seedlings infected with P. paddicum, T. incarnata and F. nivale individually, and their proteins were analyzed by SDS-PAGE. The distinctive fungal ribosomal protein bands were detected in all patterns obtained from the infected wheat plants. The possibility of the ribosomal protein-based assay to detect snow mold fungi in wheat plants is suggested.</description><identifier>ISSN: 0031-9473</identifier><identifier>EISSN: 1882-0484</identifier><identifier>DOI: 10.3186/jjphytopath.53.591</identifier><identifier>CODEN: NSBGAM</identifier><language>eng</language><publisher>Tokyo: The Phytopathological Society of Japan</publisher><subject>Biological and medical sciences ; BLE ; DIAGNOSIS ; DIAGNOSTIC ; DIAGNOSTICO ; ELECTROFORESIS ; ELECTROPHORESE ; ELECTROPHORESIS ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; fungal ribosomal proteins ; FUSARIUM ; Generalities. Techniques ; MOHO ; MOISISSURE ; MOULDS ; Phytopathology. Animal pests. 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(Hokuriku National Agricultural Experiment Station, Joetsu, Niigata (Japan))</creatorcontrib><creatorcontrib>Yoshino, R</creatorcontrib><title>Detection of snow mold fungi in wheat plants by means of polyacrylamide gel electrophoresis of ribosomal proteins</title><title>Japanese Journal of Phytopathology</title><addtitle>Jpn. J. Phytopathol.</addtitle><description>Ribosomes were isolated from four snow mold fungi (Pythium paddicum, P. iwayamai, Typhula incarnata and Fusarium nivale) and wheat seedlings, and their proteins were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). No difference was found in electrophoretic patterns between P. paddicum and P. iwayamai. However, there were several differences among two Pythium species, T. incarnata, F. nivale and wheat. The ribosomes were isolated also from wheat seedlings infected with P. paddicum, T. incarnata and F. nivale individually, and their proteins were analyzed by SDS-PAGE. The distinctive fungal ribosomal protein bands were detected in all patterns obtained from the infected wheat plants. The possibility of the ribosomal protein-based assay to detect snow mold fungi in wheat plants is suggested.</description><subject>Biological and medical sciences</subject><subject>BLE</subject><subject>DIAGNOSIS</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTICO</subject><subject>ELECTROFORESIS</subject><subject>ELECTROPHORESE</subject><subject>ELECTROPHORESIS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>fungal ribosomal proteins</subject><subject>FUSARIUM</subject><subject>Generalities. Techniques</subject><subject>MOHO</subject><subject>MOISISSURE</subject><subject>MOULDS</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>PYTHIUM</subject><subject>RIBOSOMAS</subject><subject>RIBOSOME</subject><subject>RIBOSOMES</subject><subject>snow mold fungi</subject><subject>TRIGO</subject><subject>TYPHULA</subject><subject>WHEATS</subject><issn>0031-9473</issn><issn>1882-0484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpNkE1v3CAQhlHVStkm-QM5ceh1t2CMDccqSdN8SMmhPVuzeFizwuACVeR_X282WuUyHOZ5n0EvIVecbQRXzff9fhrmEicow0aKjdT8E1lxpao1q1X9mawYE3yt61acka857xmTqpLNivy9wYKmuBhotDSH-ErH6Htq_4Wdoy7Q1wGh0MlDKJluZzoihHxgp-hnMGn2MLoe6Q49Rb-oUpyGmDC7Nyq5bcxxBE-nFAu6kC_IFws-4-X7e07-_Lz9ff1r_fR8d3_942ltqsNfLevbXjOUUjFQVrS65XVjW7Zst2B7Y6QS3KBSQgK3WvG6bhAa3NpKc4HinFRHr0kx54S2m5IbIc0dZ92htO5DaZ0U3VLaEvp2DE2QDXibIBiXT8m2ZVKrdsEej9g-F9jhaQ-pOOPxo5lrrt7s70PzE2UGSB2GxXZ1tFmIHezScvDhRWkmG6bEf4kZlko</recordid><startdate>19871225</startdate><enddate>19871225</enddate><creator>Takenaka, S. 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Plant and forest protection</topic><topic>PYTHIUM</topic><topic>RIBOSOMAS</topic><topic>RIBOSOME</topic><topic>RIBOSOMES</topic><topic>snow mold fungi</topic><topic>TRIGO</topic><topic>TYPHULA</topic><topic>WHEATS</topic><toplevel>online_resources</toplevel><creatorcontrib>Takenaka, S. (Hokuriku National Agricultural Experiment Station, Joetsu, Niigata (Japan))</creatorcontrib><creatorcontrib>Yoshino, R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Japanese Journal of Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takenaka, S. 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However, there were several differences among two Pythium species, T. incarnata, F. nivale and wheat. The ribosomes were isolated also from wheat seedlings infected with P. paddicum, T. incarnata and F. nivale individually, and their proteins were analyzed by SDS-PAGE. The distinctive fungal ribosomal protein bands were detected in all patterns obtained from the infected wheat plants. The possibility of the ribosomal protein-based assay to detect snow mold fungi in wheat plants is suggested.</abstract><cop>Tokyo</cop><pub>The Phytopathological Society of Japan</pub><doi>10.3186/jjphytopath.53.591</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Japanese Journal of Phytopathology, 1987/12/25, Vol.53(5), pp.591-597 |
| issn | 0031-9473 1882-0484 |
| language | eng |
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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences BLE DIAGNOSIS DIAGNOSTIC DIAGNOSTICO ELECTROFORESIS ELECTROPHORESE ELECTROPHORESIS Fundamental and applied biological sciences. Psychology Fungal plant pathogens fungal ribosomal proteins FUSARIUM Generalities. Techniques MOHO MOISISSURE MOULDS Phytopathology. Animal pests. Plant and forest protection PYTHIUM RIBOSOMAS RIBOSOME RIBOSOMES snow mold fungi TRIGO TYPHULA WHEATS |
| title | Detection of snow mold fungi in wheat plants by means of polyacrylamide gel electrophoresis of ribosomal proteins |
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