Comparison of the Serum Total 25-Hydroxyvitamin D Concentrations Using Chemiluminescent Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry in Children

Comparison of the Serum Total 25-Hydroxyvitamin D Concentrations Using Chemiluminescent Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry in Children

We aimed to assess the difference and agreement between the CL-series Vitamin D Total assay (Mindray), which was a kind of chemiluminescent immunoassay (CLIA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) in the measurement of serum 25-hydroxyvitamin D [25(OH)D] concentrations in chi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of Nutritional Science and Vitaminology 2022/06/30, Vol.68(3), pp.181-188
Hauptverfasser: WANG, Jie, LI, Xinliang, GAN, Yongyi, FAN, Tianxing, YANG, Jing, RAO, Fang, YANG, Jianbo
Format: Artikel
Sprache:eng
Schlagworte:
25-hydroxyvitamin D
chemiluminescent immunoassay
children
diagnosis
liquid chromatography-tandem mass spectrometry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 188
container_issue 3
container_start_page 181
container_title Journal of Nutritional Science and Vitaminology
container_volume 68
creator WANG, Jie
LI, Xinliang
GAN, Yongyi
FAN, Tianxing
YANG, Jing
RAO, Fang
YANG, Jianbo
description We aimed to assess the difference and agreement between the CL-series Vitamin D Total assay (Mindray), which was a kind of chemiluminescent immunoassay (CLIA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) in the measurement of serum 25-hydroxyvitamin D [25(OH)D] concentrations in children. We compared the 25(OH)D concentrations of 92 children using the CLIA and LC-MS/MS. Paired samples t-test was used to compare the two groups. Linear regression was used to show the correlation between CLIA and LC-MS/MS. The difference and bias between 2 methods were revealed in Bland-Altman plot. Agreement in classification of deficiency between CLIA and LC-MS/MS was assessed using Cohen’s Kappa. p value
doi_str_mv 10.3177/jnsv.68.181
format Article
fullrecord <record><control><sourceid>jstage_cross</sourceid><recordid>TN_cdi_crossref_primary_10_3177_jnsv_68_181</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>article_jnsv_68_3_68_181_article_char_en</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-dfe75430fe488b846ec24adae7f50c9a5d0bde2ecc01ad5959d629b97236fc273</originalsourceid><addsrcrecordid>eNo9kMlOwzAQQC0EEmU58QO-oxQ7q3NBQmGVijhQztHUnjSuYrvYLiK_w5eSCtTTHN6bGekRcsXZPONVdbOx4WteijkX_IjMuBA8qao8PSYzljGe5IKxU3IWwoaxvBa5mJGfxpkteB2cpa6jsUf6jn5n6NJFGGhaJM-j8u57_NIRjLb0njbOSrTRQ9TOBvoRtF3Tpkejh91kYNhT-mLMzjoIAUYKVtGF_txpNXneGYhu7WHbj8lyQmjo6-TR9y3KOFGMfqTTp6bXg_JoL8hJB0PAy_95Tj4eH5bNc7J4e3pp7haJLLI0JqrDqsgz1mEuxErkJco0BwVYdQWTNRSKrRSmKCXjoIq6qFWZ1qu6SrOyk2mVnZPrv7vSuxA8du3WawN-bDlr93nbfd62FO2Ud7Jv_-xNiLDGgws-ajngwc3-Fw5A9uBbtNkvHreKpA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Comparison of the Serum Total 25-Hydroxyvitamin D Concentrations Using Chemiluminescent Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry in Children</title><source>J-STAGE Free</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>WANG, Jie ; LI, Xinliang ; GAN, Yongyi ; FAN, Tianxing ; YANG, Jing ; RAO, Fang ; YANG, Jianbo</creator><creatorcontrib>WANG, Jie ; LI, Xinliang ; GAN, Yongyi ; FAN, Tianxing ; YANG, Jing ; RAO, Fang ; YANG, Jianbo</creatorcontrib><description>We aimed to assess the difference and agreement between the CL-series Vitamin D Total assay (Mindray), which was a kind of chemiluminescent immunoassay (CLIA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) in the measurement of serum 25-hydroxyvitamin D [25(OH)D] concentrations in children. We compared the 25(OH)D concentrations of 92 children using the CLIA and LC-MS/MS. Paired samples t-test was used to compare the two groups. Linear regression was used to show the correlation between CLIA and LC-MS/MS. The difference and bias between 2 methods were revealed in Bland-Altman plot. Agreement in classification of deficiency between CLIA and LC-MS/MS was assessed using Cohen’s Kappa. p value&lt;0.05 was considered statistically significant. Using Shapiro-Wilk Test to assess whether the data follows a normal distribution. Using 95% children’s serum 25(OH)D concentrations by LC-MS/MS as the reference interval. The regression equation was CLIA=1.185×LC-MS/MS−3.328. The fitness adjusted r2 was 0.589. The CLIA showed positive bias compared to LC-MS/MS, p&lt;0.05, bias=(1.94±16.56) ng/mL. Cohen’s Kappa=0.53, p&lt;0.001. The agreement of 2 methods in diagnosing “deficiency” was good. According to Shapiro-Wilk Test, the data followed a normal distribution (W=0.99). The reference interval of children’s serum 25(OH)D concentrations by LC-MS/MS was 11.35–44.57 ng/mL. In measuring 25(OH)D concentration of children, CLIA represented higher levels than LC-MS/MS. The two methods were consistent in diagnosing vitamin D deficiency. The reference interval of children’s serum 25(OH)D concentrations by LC-MS/MS was 11.35–44.57 ng/mL in our area in summer.</description><identifier>ISSN: 0301-4800</identifier><identifier>EISSN: 1881-7742</identifier><identifier>DOI: 10.3177/jnsv.68.181</identifier><language>eng</language><publisher>Center for Academic Publications Japan</publisher><subject>25-hydroxyvitamin D ; chemiluminescent immunoassay ; children ; diagnosis ; liquid chromatography-tandem mass spectrometry</subject><ispartof>Journal of Nutritional Science and Vitaminology, 2022/06/30, Vol.68(3), pp.181-188</ispartof><rights>2022 by the Center for Academic Publications Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-dfe75430fe488b846ec24adae7f50c9a5d0bde2ecc01ad5959d629b97236fc273</citedby><cites>FETCH-LOGICAL-c532t-dfe75430fe488b846ec24adae7f50c9a5d0bde2ecc01ad5959d629b97236fc273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids></links><search><creatorcontrib>WANG, Jie</creatorcontrib><creatorcontrib>LI, Xinliang</creatorcontrib><creatorcontrib>GAN, Yongyi</creatorcontrib><creatorcontrib>FAN, Tianxing</creatorcontrib><creatorcontrib>YANG, Jing</creatorcontrib><creatorcontrib>RAO, Fang</creatorcontrib><creatorcontrib>YANG, Jianbo</creatorcontrib><title>Comparison of the Serum Total 25-Hydroxyvitamin D Concentrations Using Chemiluminescent Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry in Children</title><title>Journal of Nutritional Science and Vitaminology</title><addtitle>J Nutr Sci Vitaminol</addtitle><description>We aimed to assess the difference and agreement between the CL-series Vitamin D Total assay (Mindray), which was a kind of chemiluminescent immunoassay (CLIA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) in the measurement of serum 25-hydroxyvitamin D [25(OH)D] concentrations in children. We compared the 25(OH)D concentrations of 92 children using the CLIA and LC-MS/MS. Paired samples t-test was used to compare the two groups. Linear regression was used to show the correlation between CLIA and LC-MS/MS. The difference and bias between 2 methods were revealed in Bland-Altman plot. Agreement in classification of deficiency between CLIA and LC-MS/MS was assessed using Cohen’s Kappa. p value&lt;0.05 was considered statistically significant. Using Shapiro-Wilk Test to assess whether the data follows a normal distribution. Using 95% children’s serum 25(OH)D concentrations by LC-MS/MS as the reference interval. The regression equation was CLIA=1.185×LC-MS/MS−3.328. The fitness adjusted r2 was 0.589. The CLIA showed positive bias compared to LC-MS/MS, p&lt;0.05, bias=(1.94±16.56) ng/mL. Cohen’s Kappa=0.53, p&lt;0.001. The agreement of 2 methods in diagnosing “deficiency” was good. According to Shapiro-Wilk Test, the data followed a normal distribution (W=0.99). The reference interval of children’s serum 25(OH)D concentrations by LC-MS/MS was 11.35–44.57 ng/mL. In measuring 25(OH)D concentration of children, CLIA represented higher levels than LC-MS/MS. The two methods were consistent in diagnosing vitamin D deficiency. The reference interval of children’s serum 25(OH)D concentrations by LC-MS/MS was 11.35–44.57 ng/mL in our area in summer.</description><subject>25-hydroxyvitamin D</subject><subject>chemiluminescent immunoassay</subject><subject>children</subject><subject>diagnosis</subject><subject>liquid chromatography-tandem mass spectrometry</subject><issn>0301-4800</issn><issn>1881-7742</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNo9kMlOwzAQQC0EEmU58QO-oxQ7q3NBQmGVijhQztHUnjSuYrvYLiK_w5eSCtTTHN6bGekRcsXZPONVdbOx4WteijkX_IjMuBA8qao8PSYzljGe5IKxU3IWwoaxvBa5mJGfxpkteB2cpa6jsUf6jn5n6NJFGGhaJM-j8u57_NIRjLb0njbOSrTRQ9TOBvoRtF3Tpkejh91kYNhT-mLMzjoIAUYKVtGF_txpNXneGYhu7WHbj8lyQmjo6-TR9y3KOFGMfqTTp6bXg_JoL8hJB0PAy_95Tj4eH5bNc7J4e3pp7haJLLI0JqrDqsgz1mEuxErkJco0BwVYdQWTNRSKrRSmKCXjoIq6qFWZ1qu6SrOyk2mVnZPrv7vSuxA8du3WawN-bDlr93nbfd62FO2Ud7Jv_-xNiLDGgws-ajngwc3-Fw5A9uBbtNkvHreKpA</recordid><startdate>20220630</startdate><enddate>20220630</enddate><creator>WANG, Jie</creator><creator>LI, Xinliang</creator><creator>GAN, Yongyi</creator><creator>FAN, Tianxing</creator><creator>YANG, Jing</creator><creator>RAO, Fang</creator><creator>YANG, Jianbo</creator><general>Center for Academic Publications Japan</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20220630</creationdate><title>Comparison of the Serum Total 25-Hydroxyvitamin D Concentrations Using Chemiluminescent Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry in Children</title><author>WANG, Jie ; LI, Xinliang ; GAN, Yongyi ; FAN, Tianxing ; YANG, Jing ; RAO, Fang ; YANG, Jianbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-dfe75430fe488b846ec24adae7f50c9a5d0bde2ecc01ad5959d629b97236fc273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>25-hydroxyvitamin D</topic><topic>chemiluminescent immunoassay</topic><topic>children</topic><topic>diagnosis</topic><topic>liquid chromatography-tandem mass spectrometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG, Jie</creatorcontrib><creatorcontrib>LI, Xinliang</creatorcontrib><creatorcontrib>GAN, Yongyi</creatorcontrib><creatorcontrib>FAN, Tianxing</creatorcontrib><creatorcontrib>YANG, Jing</creatorcontrib><creatorcontrib>RAO, Fang</creatorcontrib><creatorcontrib>YANG, Jianbo</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of Nutritional Science and Vitaminology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG, Jie</au><au>LI, Xinliang</au><au>GAN, Yongyi</au><au>FAN, Tianxing</au><au>YANG, Jing</au><au>RAO, Fang</au><au>YANG, Jianbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the Serum Total 25-Hydroxyvitamin D Concentrations Using Chemiluminescent Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry in Children</atitle><jtitle>Journal of Nutritional Science and Vitaminology</jtitle><addtitle>J Nutr Sci Vitaminol</addtitle><date>2022-06-30</date><risdate>2022</risdate><volume>68</volume><issue>3</issue><spage>181</spage><epage>188</epage><pages>181-188</pages><issn>0301-4800</issn><eissn>1881-7742</eissn><abstract>We aimed to assess the difference and agreement between the CL-series Vitamin D Total assay (Mindray), which was a kind of chemiluminescent immunoassay (CLIA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS) in the measurement of serum 25-hydroxyvitamin D [25(OH)D] concentrations in children. We compared the 25(OH)D concentrations of 92 children using the CLIA and LC-MS/MS. Paired samples t-test was used to compare the two groups. Linear regression was used to show the correlation between CLIA and LC-MS/MS. The difference and bias between 2 methods were revealed in Bland-Altman plot. Agreement in classification of deficiency between CLIA and LC-MS/MS was assessed using Cohen’s Kappa. p value&lt;0.05 was considered statistically significant. Using Shapiro-Wilk Test to assess whether the data follows a normal distribution. Using 95% children’s serum 25(OH)D concentrations by LC-MS/MS as the reference interval. The regression equation was CLIA=1.185×LC-MS/MS−3.328. The fitness adjusted r2 was 0.589. The CLIA showed positive bias compared to LC-MS/MS, p&lt;0.05, bias=(1.94±16.56) ng/mL. Cohen’s Kappa=0.53, p&lt;0.001. The agreement of 2 methods in diagnosing “deficiency” was good. According to Shapiro-Wilk Test, the data followed a normal distribution (W=0.99). The reference interval of children’s serum 25(OH)D concentrations by LC-MS/MS was 11.35–44.57 ng/mL. In measuring 25(OH)D concentration of children, CLIA represented higher levels than LC-MS/MS. The two methods were consistent in diagnosing vitamin D deficiency. The reference interval of children’s serum 25(OH)D concentrations by LC-MS/MS was 11.35–44.57 ng/mL in our area in summer.</abstract><pub>Center for Academic Publications Japan</pub><doi>10.3177/jnsv.68.181</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0301-4800
ispartof Journal of Nutritional Science and Vitaminology, 2022/06/30, Vol.68(3), pp.181-188
issn 0301-4800
1881-7742
language eng
recordid cdi_crossref_primary_10_3177_jnsv_68_181
source J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects 25-hydroxyvitamin D
chemiluminescent immunoassay
children
diagnosis
liquid chromatography-tandem mass spectrometry
title Comparison of the Serum Total 25-Hydroxyvitamin D Concentrations Using Chemiluminescent Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry in Children
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T23%3A06%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstage_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20the%20Serum%20Total%2025-Hydroxyvitamin%20D%20Concentrations%20Using%20Chemiluminescent%20Immunoassay%20and%20Liquid%20Chromatography-Tandem%20Mass%20Spectrometry%20in%20Children&rft.jtitle=Journal%20of%20Nutritional%20Science%20and%20Vitaminology&rft.au=WANG,%20Jie&rft.date=2022-06-30&rft.volume=68&rft.issue=3&rft.spage=181&rft.epage=188&rft.pages=181-188&rft.issn=0301-4800&rft.eissn=1881-7742&rft_id=info:doi/10.3177/jnsv.68.181&rft_dat=%3Cjstage_cross%3Earticle_jnsv_68_3_68_181_article_char_en%3C/jstage_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true