Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone
A determination method for individual natural vitamin B6 compounds was developed. The vitamin B6 compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyrido...
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| Veröffentlicht in: | Journal of Nutritional Science and Vitaminology 2008, Vol.54(1), pp.18-24 |
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| container_title | Journal of Nutritional Science and Vitaminology |
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| creator | Nishimura, S.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture) Nagano, S Crai, C.A Yokochi, N Toshikane, Y Ge, F Yagi, T |
| description | A determination method for individual natural vitamin B6 compounds was developed. The vitamin B6 compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-beta-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method. |
| doi_str_mv | 10.3177/jnsv.54.18 |
| format | Article |
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Faculty of Agriculture) ; Nagano, S ; Crai, C.A ; Yokochi, N ; Toshikane, Y ; Ge, F ; Yagi, T</creator><creatorcontrib>Nishimura, S.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture) ; Nagano, S ; Crai, C.A ; Yokochi, N ; Toshikane, Y ; Ge, F ; Yagi, T</creatorcontrib><description>A determination method for individual natural vitamin B6 compounds was developed. The vitamin B6 compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-beta-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method.</description><identifier>ISSN: 0301-4800</identifier><identifier>EISSN: 1881-7742</identifier><identifier>DOI: 10.3177/jnsv.54.18</identifier><language>eng</language><publisher>Center for Academic Publications Japan</publisher><subject>4-pyridoxolactone ; ACIDE CHLORHYDRIQUE ; ACIDO CLORHIDRICO ; ALIMENTOS ; ANALYTICAL METHODS ; ECHANTILLONNAGE ; ENZIMAS ; ENZYME ; ENZYMES ; FLUORESCENCE ; FLUORESCENCIA ; FOODS ; HPLC ; HYDROCHLORIC ACID ; LACTONAS ; LACTONE ; LACTONES ; MUESTREO ; PIRIDOXINA ; PRODUIT ALIMENTAIRE ; pyridoxal 4-dehydrogenase ; pyridoxamine-pyruvate aminotransferase ; PYRIDOXINE ; pyridoxine 4-oxidase ; SAMPLING ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; VARIEDADES ; VARIETE ; VARIETIES ; vitamin B6</subject><ispartof>Journal of Nutritional Science and Vitaminology, 2008, Vol.54(1), pp.18-24</ispartof><rights>2008 by the Center for Academic Publications Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-18de727d0a082c83ad88be876aef43bafbd8096e654d6241e57cd7b4321de80b3</citedby><cites>FETCH-LOGICAL-c440t-18de727d0a082c83ad88be876aef43bafbd8096e654d6241e57cd7b4321de80b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids></links><search><creatorcontrib>Nishimura, S.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Nagano, S</creatorcontrib><creatorcontrib>Crai, C.A</creatorcontrib><creatorcontrib>Yokochi, N</creatorcontrib><creatorcontrib>Toshikane, Y</creatorcontrib><creatorcontrib>Ge, F</creatorcontrib><creatorcontrib>Yagi, T</creatorcontrib><title>Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone</title><title>Journal of Nutritional Science and Vitaminology</title><addtitle>J Nutr Sci Vitaminol</addtitle><description>A determination method for individual natural vitamin B6 compounds was developed. The vitamin B6 compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-beta-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method.</description><subject>4-pyridoxolactone</subject><subject>ACIDE CHLORHYDRIQUE</subject><subject>ACIDO CLORHIDRICO</subject><subject>ALIMENTOS</subject><subject>ANALYTICAL METHODS</subject><subject>ECHANTILLONNAGE</subject><subject>ENZIMAS</subject><subject>ENZYME</subject><subject>ENZYMES</subject><subject>FLUORESCENCE</subject><subject>FLUORESCENCIA</subject><subject>FOODS</subject><subject>HPLC</subject><subject>HYDROCHLORIC ACID</subject><subject>LACTONAS</subject><subject>LACTONE</subject><subject>LACTONES</subject><subject>MUESTREO</subject><subject>PIRIDOXINA</subject><subject>PRODUIT ALIMENTAIRE</subject><subject>pyridoxal 4-dehydrogenase</subject><subject>pyridoxamine-pyruvate aminotransferase</subject><subject>PYRIDOXINE</subject><subject>pyridoxine 4-oxidase</subject><subject>SAMPLING</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>VARIEDADES</subject><subject>VARIETE</subject><subject>VARIETIES</subject><subject>vitamin B6</subject><issn>0301-4800</issn><issn>1881-7742</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNpFkD1PwzAQhi0EEqWwsCN5RkqxEyd2J8T3hyrBALPl2JfiKrErO40ovx5XQWW5G97nHulehM4pmRWU86uVi8OsZDMqDtCECkEzzll-iCakIDRjgpBjdBLjihA2F0xMkL6HHkJnneqtd9g32DpjB2s2qsWD7VWK8G2Fte_WfuNMxLWKYHBiwf1su3SmU-gGCHEn6D1m2XobrPHfvlW69w5O0VGj2ghnf3uKPh8fPu6es8Xb08vdzSLTjJE-o8IAz7khiohci0IZIWoQvFLQsKJWTW0EmVdQlcxUOaNQcm14zYqcGhCkLqbocvTq4GMM0Mh1sJ0KW0mJ3NUjd_XIkkkqEnw9wqvYqyXsURXSRy38o-OgYp_oLxUkuGS4GA2N8lItg43y9T0nJJXMCafFL_4Ue14</recordid><startdate>200802</startdate><enddate>200802</enddate><creator>Nishimura, S.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture)</creator><creator>Nagano, S</creator><creator>Crai, C.A</creator><creator>Yokochi, N</creator><creator>Toshikane, Y</creator><creator>Ge, F</creator><creator>Yagi, T</creator><general>Center for Academic Publications Japan</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200802</creationdate><title>Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone</title><author>Nishimura, S.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture) ; Nagano, S ; Crai, C.A ; Yokochi, N ; Toshikane, Y ; Ge, F ; Yagi, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-18de727d0a082c83ad88be876aef43bafbd8096e654d6241e57cd7b4321de80b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>4-pyridoxolactone</topic><topic>ACIDE CHLORHYDRIQUE</topic><topic>ACIDO CLORHIDRICO</topic><topic>ALIMENTOS</topic><topic>ANALYTICAL METHODS</topic><topic>ECHANTILLONNAGE</topic><topic>ENZIMAS</topic><topic>ENZYME</topic><topic>ENZYMES</topic><topic>FLUORESCENCE</topic><topic>FLUORESCENCIA</topic><topic>FOODS</topic><topic>HPLC</topic><topic>HYDROCHLORIC ACID</topic><topic>LACTONAS</topic><topic>LACTONE</topic><topic>LACTONES</topic><topic>MUESTREO</topic><topic>PIRIDOXINA</topic><topic>PRODUIT ALIMENTAIRE</topic><topic>pyridoxal 4-dehydrogenase</topic><topic>pyridoxamine-pyruvate aminotransferase</topic><topic>PYRIDOXINE</topic><topic>pyridoxine 4-oxidase</topic><topic>SAMPLING</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>VARIEDADES</topic><topic>VARIETE</topic><topic>VARIETIES</topic><topic>vitamin B6</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishimura, S.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture)</creatorcontrib><creatorcontrib>Nagano, S</creatorcontrib><creatorcontrib>Crai, C.A</creatorcontrib><creatorcontrib>Yokochi, N</creatorcontrib><creatorcontrib>Toshikane, Y</creatorcontrib><creatorcontrib>Ge, F</creatorcontrib><creatorcontrib>Yagi, T</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Journal of Nutritional Science and Vitaminology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishimura, S.(Kochi Univ., Nankoku (Japan). Faculty of Agriculture)</au><au>Nagano, S</au><au>Crai, C.A</au><au>Yokochi, N</au><au>Toshikane, Y</au><au>Ge, F</au><au>Yagi, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone</atitle><jtitle>Journal of Nutritional Science and Vitaminology</jtitle><addtitle>J Nutr Sci Vitaminol</addtitle><date>2008-02</date><risdate>2008</risdate><volume>54</volume><issue>1</issue><spage>18</spage><epage>24</epage><pages>18-24</pages><issn>0301-4800</issn><eissn>1881-7742</eissn><abstract>A determination method for individual natural vitamin B6 compounds was developed. The vitamin B6 compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-beta-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-beta-glucoside were not separately determined here. Three food samples were analyzed by this method.</abstract><pub>Center for Academic Publications Japan</pub><doi>10.3177/jnsv.54.18</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 4-pyridoxolactone ACIDE CHLORHYDRIQUE ACIDO CLORHIDRICO ALIMENTOS ANALYTICAL METHODS ECHANTILLONNAGE ENZIMAS ENZYME ENZYMES FLUORESCENCE FLUORESCENCIA FOODS HPLC HYDROCHLORIC ACID LACTONAS LACTONE LACTONES MUESTREO PIRIDOXINA PRODUIT ALIMENTAIRE pyridoxal 4-dehydrogenase pyridoxamine-pyruvate aminotransferase PYRIDOXINE pyridoxine 4-oxidase SAMPLING TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS VARIEDADES VARIETE VARIETIES vitamin B6 |
| title | Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone |
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