Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay
Enzyme linked immunosorbent assay (ELISA) using the anti-masu Ig rabbit serum was applied to detect the antibody against Aeromonas salmonicida in the sera of salmonid fish. This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) i...
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| Veröffentlicht in: | Fish Pathology 1992/06/15, Vol.27(2), pp.73-82 |
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| container_title | Fish Pathology |
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| creator | Yoshimizu, M. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries) Direkbusarakom, S Nomura, T Ezura, Y Kimura, T |
| description | Enzyme linked immunosorbent assay (ELISA) using the anti-masu Ig rabbit serum was applied to detect the antibody against Aeromonas salmonicida in the sera of salmonid fish. This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) immunized by A. salmonicida and could be applied to detect the antibody against A. salmonicida in sera of matured masu, pink (O. gorbuscha), chum, and kokanee salmon (O. nerka). In 1989, 89 masu and 250 chum salmon, and 59 kokanee were examined to detect antibody against A. salmonicida by ELISA and agglutination test. The number of the fish showing the positive reaction by ELISA, agglutination test and isolation of A. salmonicida were 262, 48, and 33, respectively. In 1990, 200 masu and kokanee salmon fry released from hatchery to river were tested and antibody against A. salmonicida was detected from 111 fish by ELISA, and the incidence of the fish showing the positive reaction was ranged from 4 to 90 %. |
| doi_str_mv | 10.3147/jsfp.27.73 |
| format | Article |
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(Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries) ; Direkbusarakom, S ; Nomura, T ; Ezura, Y ; Kimura, T</creator><creatorcontrib>Yoshimizu, M. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries) ; Direkbusarakom, S ; Nomura, T ; Ezura, Y ; Kimura, T</creatorcontrib><description>Enzyme linked immunosorbent assay (ELISA) using the anti-masu Ig rabbit serum was applied to detect the antibody against Aeromonas salmonicida in the sera of salmonid fish. This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) immunized by A. salmonicida and could be applied to detect the antibody against A. salmonicida in sera of matured masu, pink (O. gorbuscha), chum, and kokanee salmon (O. nerka). In 1989, 89 masu and 250 chum salmon, and 59 kokanee were examined to detect antibody against A. salmonicida by ELISA and agglutination test. The number of the fish showing the positive reaction by ELISA, agglutination test and isolation of A. salmonicida were 262, 48, and 33, respectively. In 1990, 200 masu and kokanee salmon fry released from hatchery to river were tested and antibody against A. salmonicida was detected from 111 fish by ELISA, and the incidence of the fish showing the positive reaction was ranged from 4 to 90 %.</description><identifier>ISSN: 0388-788X</identifier><identifier>EISSN: 1881-7335</identifier><identifier>DOI: 10.3147/jsfp.27.73</identifier><language>eng</language><publisher>The Japanese Society of Fish Pathology</publisher><subject>AEROMONAS SALMONICIDA ; ANTIBODIES ; ANTICORPS ; ANTICUERPOS ; BLOOD SERUM ; CONEJO (ORYCTOLAGUS) ; ELISA ; IMMUNOGLOBULINE ; IMMUNOGLOBULINS ; INMUNOGLOBULINA ; LAPIN (ORYCTOLAGUS) ; ONCORHYNCHUS MASOU ; RABBITS ; SALMONOIDEI ; SERUM SANGUIN ; SUERO SANGUINEO ; TEST ELISA</subject><ispartof>Fish Pathology, 1992/06/15, Vol.27(2), pp.73-82</ispartof><rights>The Japanese Society of Fish Pathology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c320t-9fa8248dd94559bb2c3ce865fa1a3a4edf689bf52321d31d98aed03165e487943</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids></links><search><creatorcontrib>Yoshimizu, M. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries)</creatorcontrib><creatorcontrib>Direkbusarakom, S</creatorcontrib><creatorcontrib>Nomura, T</creatorcontrib><creatorcontrib>Ezura, Y</creatorcontrib><creatorcontrib>Kimura, T</creatorcontrib><title>Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay</title><title>Fish Pathology</title><addtitle>Fish Pathol.</addtitle><description>Enzyme linked immunosorbent assay (ELISA) using the anti-masu Ig rabbit serum was applied to detect the antibody against Aeromonas salmonicida in the sera of salmonid fish. This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) immunized by A. salmonicida and could be applied to detect the antibody against A. salmonicida in sera of matured masu, pink (O. gorbuscha), chum, and kokanee salmon (O. nerka). In 1989, 89 masu and 250 chum salmon, and 59 kokanee were examined to detect antibody against A. salmonicida by ELISA and agglutination test. The number of the fish showing the positive reaction by ELISA, agglutination test and isolation of A. salmonicida were 262, 48, and 33, respectively. In 1990, 200 masu and kokanee salmon fry released from hatchery to river were tested and antibody against A. salmonicida was detected from 111 fish by ELISA, and the incidence of the fish showing the positive reaction was ranged from 4 to 90 %.</description><subject>AEROMONAS SALMONICIDA</subject><subject>ANTIBODIES</subject><subject>ANTICORPS</subject><subject>ANTICUERPOS</subject><subject>BLOOD SERUM</subject><subject>CONEJO (ORYCTOLAGUS)</subject><subject>ELISA</subject><subject>IMMUNOGLOBULINE</subject><subject>IMMUNOGLOBULINS</subject><subject>INMUNOGLOBULINA</subject><subject>LAPIN (ORYCTOLAGUS)</subject><subject>ONCORHYNCHUS MASOU</subject><subject>RABBITS</subject><subject>SALMONOIDEI</subject><subject>SERUM SANGUIN</subject><subject>SUERO SANGUINEO</subject><subject>TEST ELISA</subject><issn>0388-788X</issn><issn>1881-7335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNo9kLtuGzEQRYkgAaIoblKmYm1gZXJnH2RpyI_EMJAUCeCOmF0OJcpa0iDpYv31lizDzcwA58wtLmM_pFiBbPqLXXZPq7pf9fCJLaRSsuoB2s9sIUCpqlfq4Sv7lvNOiLYDAQv2ckWFxuJj4NFxDMUP0c4cN-hDLvySUpxiwMwz7g-HH71F7gMvW-KZ0vN0fHtnljuft3yY3yiFl3kivvfhkSz30_QcYo5poFA45ozzd_bF4T7T2ftesv831__Wv6r7P7e_15f31Qi1KJV2qOpGWaubttXDUI8wkupahxIBG7KuU3pwbQ21tCCtVkhWgOxaalSvG1iy81PumGLOiZx5Sn7CNBspzLE1c2zN1L05dLVk65O8ywU39KFiKn7c05sqddcd9fo0evig4xaToXBI-XlKcRgNbpLP5u6v7gToRsArTaWD0w</recordid><startdate>19920601</startdate><enddate>19920601</enddate><creator>Yoshimizu, M. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries)</creator><creator>Direkbusarakom, S</creator><creator>Nomura, T</creator><creator>Ezura, Y</creator><creator>Kimura, T</creator><general>The Japanese Society of Fish Pathology</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19920601</creationdate><title>Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay</title><author>Yoshimizu, M. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries) ; Direkbusarakom, S ; Nomura, T ; Ezura, Y ; Kimura, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c320t-9fa8248dd94559bb2c3ce865fa1a3a4edf689bf52321d31d98aed03165e487943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>AEROMONAS SALMONICIDA</topic><topic>ANTIBODIES</topic><topic>ANTICORPS</topic><topic>ANTICUERPOS</topic><topic>BLOOD SERUM</topic><topic>CONEJO (ORYCTOLAGUS)</topic><topic>ELISA</topic><topic>IMMUNOGLOBULINE</topic><topic>IMMUNOGLOBULINS</topic><topic>INMUNOGLOBULINA</topic><topic>LAPIN (ORYCTOLAGUS)</topic><topic>ONCORHYNCHUS MASOU</topic><topic>RABBITS</topic><topic>SALMONOIDEI</topic><topic>SERUM SANGUIN</topic><topic>SUERO SANGUINEO</topic><topic>TEST ELISA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshimizu, M. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries)</creatorcontrib><creatorcontrib>Direkbusarakom, S</creatorcontrib><creatorcontrib>Nomura, T</creatorcontrib><creatorcontrib>Ezura, Y</creatorcontrib><creatorcontrib>Kimura, T</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Fish Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshimizu, M. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries)</au><au>Direkbusarakom, S</au><au>Nomura, T</au><au>Ezura, Y</au><au>Kimura, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay</atitle><jtitle>Fish Pathology</jtitle><addtitle>Fish Pathol.</addtitle><date>1992-06-01</date><risdate>1992</risdate><volume>27</volume><issue>2</issue><spage>73</spage><epage>82</epage><pages>73-82</pages><issn>0388-788X</issn><eissn>1881-7335</eissn><abstract>Enzyme linked immunosorbent assay (ELISA) using the anti-masu Ig rabbit serum was applied to detect the antibody against Aeromonas salmonicida in the sera of salmonid fish. This ELISA method could detect the antibody in sera of masu (Oncorhynchus masou), chum (O. keta) and coho salmon (O. kisutch) immunized by A. salmonicida and could be applied to detect the antibody against A. salmonicida in sera of matured masu, pink (O. gorbuscha), chum, and kokanee salmon (O. nerka). In 1989, 89 masu and 250 chum salmon, and 59 kokanee were examined to detect antibody against A. salmonicida by ELISA and agglutination test. The number of the fish showing the positive reaction by ELISA, agglutination test and isolation of A. salmonicida were 262, 48, and 33, respectively. In 1990, 200 masu and kokanee salmon fry released from hatchery to river were tested and antibody against A. salmonicida was detected from 111 fish by ELISA, and the incidence of the fish showing the positive reaction was ranged from 4 to 90 %.</abstract><pub>The Japanese Society of Fish Pathology</pub><doi>10.3147/jsfp.27.73</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0388-788X |
| ispartof | Fish Pathology, 1992/06/15, Vol.27(2), pp.73-82 |
| issn | 0388-788X 1881-7335 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | AEROMONAS SALMONICIDA ANTIBODIES ANTICORPS ANTICUERPOS BLOOD SERUM CONEJO (ORYCTOLAGUS) ELISA IMMUNOGLOBULINE IMMUNOGLOBULINS INMUNOGLOBULINA LAPIN (ORYCTOLAGUS) ONCORHYNCHUS MASOU RABBITS SALMONOIDEI SERUM SANGUIN SUERO SANGUINEO TEST ELISA |
| title | Detection of antibody against Aeromonas salmonicida in the serum of salmonid fish by the enzyme linked immunosorbent assay |
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