In Vitro Studies of Cytotoxic Effect on Sarcoma-180 Cells of β-Subunit from Egg White Ovomucin

In Vitro Studies of Cytotoxic Effect on Sarcoma-180 Cells of β-Subunit from Egg White Ovomucin

When sarcoma-180 (SR-180) cells were incubated with α- or β-subunit prepared from insoluble ovomucin in the gel fraction of egg white, the cell proliferation rate was apparently reduced by the addition of β-subunit, while it was largely unaffected by α-subunit. Examinations by scanning and transmiss...

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Veröffentlicht in:Food Science and Technology Research 1999, Vol.5(3), pp.273-278
Hauptverfasser: YOKOTA, Takashi, OHISHI, Hifumi, WATANABE, Kenji
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Sprache:eng
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cytotoxic effect
egg white protein
electron microscopy
ovomucin
sarcoma-180 cells
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WATANABE, Kenji
description When sarcoma-180 (SR-180) cells were incubated with α- or β-subunit prepared from insoluble ovomucin in the gel fraction of egg white, the cell proliferation rate was apparently reduced by the addition of β-subunit, while it was largely unaffected by α-subunit. Examinations by scanning and transmission electronmicroscopy indicated a dose- and time-dependent cytotoxic effect of β-subunit on SR-180 cells, whereas β-subunit did not injure mouse peritoneum macrophage used as normal cells. Ultrastructurally, SR-180 cells treated with β-subunit showed changes such as swelling and bleb formation of microvilli on cell membrane, irregular clumping of chromatin, irregular nuclear shape, and marked swelling of organelles in cytoplasm associated with cell degeneration in necrotic change. Furthermore, apoptotic analysis of the cultured SR-180 cells also demonstrated that β-subunit did not induce the apoptotic DNA fragmentation.
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subjects cytotoxic effect
egg white protein
electron microscopy
ovomucin
sarcoma-180 cells
title In Vitro Studies of Cytotoxic Effect on Sarcoma-180 Cells of β-Subunit from Egg White Ovomucin
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