Modulation of microRNAs expression in hematopoietic stem cells treated with sodium butyrate in inducing fetal hemoglobin expression
Context Inherited hemoglobin diseases are the most common single-gene disorders. Induction of fetal hemoglobin in beta hemoglobin disorders compensate for abnormal chain and ameliorate the clinical complications. Sodium butyrate is used conventionally for fetal hemoglobin induction; it can be replac...
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| creator | Tayebi, Behnoosh Abrishami, Fatemeh Alizadeh, Shaban Minayi, Neda Mohammadian, Mozhdeh Soleimani, Masoud Dehghanifard, Ali Atwan, Hossein Ajami, Monireh Ajami, Mansoureh |
| description | Context Inherited hemoglobin diseases are the most common single-gene disorders. Induction of fetal hemoglobin in beta hemoglobin disorders compensate for abnormal chain and ameliorate the clinical complications. Sodium butyrate is used conventionally for fetal hemoglobin induction; it can be replaced by safer therapeutic tools like microRNAs, small non-coding RNAs that control number of epigenetic mechanisms. Objective In this study, we compared the changes in the microRNAs of differentiated erythroid cells between control and sodium butyrate treated groups. The objective is to find significant association between these changes and gamma chain up regulation. Materials and methods First, CD133
+
hematopoietic stem cells were isolated from cord blood by magnetic cell sorting (MACS) technique. After proliferation, the cells were differentiated to erythroid lineage in culture medium by EPO, SCF, and IL3. Meanwhile, the test group was treated with sodium butyrate. Then, gamma chain upregulation was verified by qPCR technique. Finally, microRNA profiling was performed through microarray assay and some of them confirmed by qPCR. Result Results demonstrated that gamma chain was 5.9-fold upregulated in the treated group. Significant changes were observed at 76 microRNAs, in which 20 were up-regulated and 56 were down-regulated. Discussion Five of these microRNAs including U101, hsa-miR-4726-5p, hsa-miR7109 5p, hsa-miR3663, and hsa-miR940 had significant changes in expression and volume. Conclusion In conclusion, it can be assumed that sodium butyrate can up-regulate gamma chain gene, and change miRNAs expression. These results can be profitable in future studies to find therapeutic goal suitable for such disorders. |
| doi_str_mv | 10.3109/21691401.2016.1138487 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_3109_21691401_2016_1138487</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4276555981</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-e0d49d43bf1c0e8bc73783d69abe50c60ff759f3ef5fc092561b7985df2b949d3</originalsourceid><addsrcrecordid>eNp9kEtrGzEUhUVJaULqn9Ai6NqO7ry1qzFJG8gDSgvdCY10FcvMjFxJg-N1_ng12HF20Ubi6JxzuR8hX4AtcmD8KoOKQ8FgkTGoFgB5UzT1B3Ix6XMo4O_Z6c3gnMxC2LB0GqjqsvhEzrOqyeuGwwV5uXd67GS0bqDO0N4q7349LAPF563HECbdDnSNvYxu6yxGq2iI2FOFXRdo9CgjarqzcU2D03bsaTvGvU_qFLSDHpUdnqjBKLupxz11rk0_bwM-k49GdgFnx_uS_Lm5_r36Ob97_HG7Wt7NVVFAnCPTBddF3hpQDJtW1WmHXFdctlgyVTFj6pKbHE1pFONZWUFb86bUJmt5SuaX5Nuhd-vdvxFDFBs3-iGNFJAAAiSIeXKVB1ciEYJHI7be9tLvBTAx0Rev9MVEXxzpp9zXY_vY9qhPqVfWyfD9YLCDcb6XO-c7LaLcd84bLwdlw9T_3oz_aRqW2Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1848114873</pqid></control><display><type>article</type><title>Modulation of microRNAs expression in hematopoietic stem cells treated with sodium butyrate in inducing fetal hemoglobin expression</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Tayebi, Behnoosh ; Abrishami, Fatemeh ; Alizadeh, Shaban ; Minayi, Neda ; Mohammadian, Mozhdeh ; Soleimani, Masoud ; Dehghanifard, Ali ; Atwan, Hossein ; Ajami, Monireh ; Ajami, Mansoureh</creator><creatorcontrib>Tayebi, Behnoosh ; Abrishami, Fatemeh ; Alizadeh, Shaban ; Minayi, Neda ; Mohammadian, Mozhdeh ; Soleimani, Masoud ; Dehghanifard, Ali ; Atwan, Hossein ; Ajami, Monireh ; Ajami, Mansoureh</creatorcontrib><description>Context Inherited hemoglobin diseases are the most common single-gene disorders. Induction of fetal hemoglobin in beta hemoglobin disorders compensate for abnormal chain and ameliorate the clinical complications. Sodium butyrate is used conventionally for fetal hemoglobin induction; it can be replaced by safer therapeutic tools like microRNAs, small non-coding RNAs that control number of epigenetic mechanisms. Objective In this study, we compared the changes in the microRNAs of differentiated erythroid cells between control and sodium butyrate treated groups. The objective is to find significant association between these changes and gamma chain up regulation. Materials and methods First, CD133
+
hematopoietic stem cells were isolated from cord blood by magnetic cell sorting (MACS) technique. After proliferation, the cells were differentiated to erythroid lineage in culture medium by EPO, SCF, and IL3. Meanwhile, the test group was treated with sodium butyrate. Then, gamma chain upregulation was verified by qPCR technique. Finally, microRNA profiling was performed through microarray assay and some of them confirmed by qPCR. Result Results demonstrated that gamma chain was 5.9-fold upregulated in the treated group. Significant changes were observed at 76 microRNAs, in which 20 were up-regulated and 56 were down-regulated. Discussion Five of these microRNAs including U101, hsa-miR-4726-5p, hsa-miR7109 5p, hsa-miR3663, and hsa-miR940 had significant changes in expression and volume. Conclusion In conclusion, it can be assumed that sodium butyrate can up-regulate gamma chain gene, and change miRNAs expression. These results can be profitable in future studies to find therapeutic goal suitable for such disorders.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.3109/21691401.2016.1138487</identifier><identifier>PMID: 26837891</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Butyric Acid - pharmacology ; Cell culture ; Cell differentiation ; Cell proliferation ; Cells, Cultured ; Chains ; Cord blood ; Disorders ; DNA microarrays ; Epigenetics ; Erythroid cells ; Fetal hemoglobin ; Fetal Hemoglobin - biosynthesis ; Fetal Hemoglobin - genetics ; Fetuses ; Gene expression ; Gene Expression Regulation - drug effects ; Genes ; hematopoietic stem cell ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - metabolism ; Hemoglobin ; Humans ; Interleukin 3 ; microarray ; microRNA ; MicroRNAs ; MicroRNAs - biosynthesis ; MicroRNAs - genetics ; miRNA ; Ribonucleic acid ; RNA ; sickle cell anemia ; Sodium ; Sodium butyrate ; Stem cells ; thalassemia</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2017-02, Vol.45 (1), p.146-156</ispartof><rights>2016 Taylor & Francis 2016</rights><rights>2016 Taylor & Francis</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-e0d49d43bf1c0e8bc73783d69abe50c60ff759f3ef5fc092561b7985df2b949d3</citedby><cites>FETCH-LOGICAL-c441t-e0d49d43bf1c0e8bc73783d69abe50c60ff759f3ef5fc092561b7985df2b949d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26837891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tayebi, Behnoosh</creatorcontrib><creatorcontrib>Abrishami, Fatemeh</creatorcontrib><creatorcontrib>Alizadeh, Shaban</creatorcontrib><creatorcontrib>Minayi, Neda</creatorcontrib><creatorcontrib>Mohammadian, Mozhdeh</creatorcontrib><creatorcontrib>Soleimani, Masoud</creatorcontrib><creatorcontrib>Dehghanifard, Ali</creatorcontrib><creatorcontrib>Atwan, Hossein</creatorcontrib><creatorcontrib>Ajami, Monireh</creatorcontrib><creatorcontrib>Ajami, Mansoureh</creatorcontrib><title>Modulation of microRNAs expression in hematopoietic stem cells treated with sodium butyrate in inducing fetal hemoglobin expression</title><title>Artificial cells, nanomedicine, and biotechnology</title><addtitle>Artif Cells Nanomed Biotechnol</addtitle><description>Context Inherited hemoglobin diseases are the most common single-gene disorders. Induction of fetal hemoglobin in beta hemoglobin disorders compensate for abnormal chain and ameliorate the clinical complications. Sodium butyrate is used conventionally for fetal hemoglobin induction; it can be replaced by safer therapeutic tools like microRNAs, small non-coding RNAs that control number of epigenetic mechanisms. Objective In this study, we compared the changes in the microRNAs of differentiated erythroid cells between control and sodium butyrate treated groups. The objective is to find significant association between these changes and gamma chain up regulation. Materials and methods First, CD133
+
hematopoietic stem cells were isolated from cord blood by magnetic cell sorting (MACS) technique. After proliferation, the cells were differentiated to erythroid lineage in culture medium by EPO, SCF, and IL3. Meanwhile, the test group was treated with sodium butyrate. Then, gamma chain upregulation was verified by qPCR technique. Finally, microRNA profiling was performed through microarray assay and some of them confirmed by qPCR. Result Results demonstrated that gamma chain was 5.9-fold upregulated in the treated group. Significant changes were observed at 76 microRNAs, in which 20 were up-regulated and 56 were down-regulated. Discussion Five of these microRNAs including U101, hsa-miR-4726-5p, hsa-miR7109 5p, hsa-miR3663, and hsa-miR940 had significant changes in expression and volume. Conclusion In conclusion, it can be assumed that sodium butyrate can up-regulate gamma chain gene, and change miRNAs expression. These results can be profitable in future studies to find therapeutic goal suitable for such disorders.</description><subject>Butyric Acid - pharmacology</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell proliferation</subject><subject>Cells, Cultured</subject><subject>Chains</subject><subject>Cord blood</subject><subject>Disorders</subject><subject>DNA microarrays</subject><subject>Epigenetics</subject><subject>Erythroid cells</subject><subject>Fetal hemoglobin</subject><subject>Fetal Hemoglobin - biosynthesis</subject><subject>Fetal Hemoglobin - genetics</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes</subject><subject>hematopoietic stem cell</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Hemoglobin</subject><subject>Humans</subject><subject>Interleukin 3</subject><subject>microarray</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - biosynthesis</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>sickle cell anemia</subject><subject>Sodium</subject><subject>Sodium butyrate</subject><subject>Stem cells</subject><subject>thalassemia</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtrGzEUhUVJaULqn9Ai6NqO7ry1qzFJG8gDSgvdCY10FcvMjFxJg-N1_ng12HF20Ubi6JxzuR8hX4AtcmD8KoOKQ8FgkTGoFgB5UzT1B3Ix6XMo4O_Z6c3gnMxC2LB0GqjqsvhEzrOqyeuGwwV5uXd67GS0bqDO0N4q7349LAPF563HECbdDnSNvYxu6yxGq2iI2FOFXRdo9CgjarqzcU2D03bsaTvGvU_qFLSDHpUdnqjBKLupxz11rk0_bwM-k49GdgFnx_uS_Lm5_r36Ob97_HG7Wt7NVVFAnCPTBddF3hpQDJtW1WmHXFdctlgyVTFj6pKbHE1pFONZWUFb86bUJmt5SuaX5Nuhd-vdvxFDFBs3-iGNFJAAAiSIeXKVB1ciEYJHI7be9tLvBTAx0Rev9MVEXxzpp9zXY_vY9qhPqVfWyfD9YLCDcb6XO-c7LaLcd84bLwdlw9T_3oz_aRqW2Q</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Tayebi, Behnoosh</creator><creator>Abrishami, Fatemeh</creator><creator>Alizadeh, Shaban</creator><creator>Minayi, Neda</creator><creator>Mohammadian, Mozhdeh</creator><creator>Soleimani, Masoud</creator><creator>Dehghanifard, Ali</creator><creator>Atwan, Hossein</creator><creator>Ajami, Monireh</creator><creator>Ajami, Mansoureh</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20170201</creationdate><title>Modulation of microRNAs expression in hematopoietic stem cells treated with sodium butyrate in inducing fetal hemoglobin expression</title><author>Tayebi, Behnoosh ; Abrishami, Fatemeh ; Alizadeh, Shaban ; Minayi, Neda ; Mohammadian, Mozhdeh ; Soleimani, Masoud ; Dehghanifard, Ali ; Atwan, Hossein ; Ajami, Monireh ; Ajami, Mansoureh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-e0d49d43bf1c0e8bc73783d69abe50c60ff759f3ef5fc092561b7985df2b949d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Butyric Acid - pharmacology</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Cell proliferation</topic><topic>Cells, Cultured</topic><topic>Chains</topic><topic>Cord blood</topic><topic>Disorders</topic><topic>DNA microarrays</topic><topic>Epigenetics</topic><topic>Erythroid cells</topic><topic>Fetal hemoglobin</topic><topic>Fetal Hemoglobin - biosynthesis</topic><topic>Fetal Hemoglobin - genetics</topic><topic>Fetuses</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genes</topic><topic>hematopoietic stem cell</topic><topic>Hematopoietic stem cells</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Hemoglobin</topic><topic>Humans</topic><topic>Interleukin 3</topic><topic>microarray</topic><topic>microRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - biosynthesis</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>sickle cell anemia</topic><topic>Sodium</topic><topic>Sodium butyrate</topic><topic>Stem cells</topic><topic>thalassemia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tayebi, Behnoosh</creatorcontrib><creatorcontrib>Abrishami, Fatemeh</creatorcontrib><creatorcontrib>Alizadeh, Shaban</creatorcontrib><creatorcontrib>Minayi, Neda</creatorcontrib><creatorcontrib>Mohammadian, Mozhdeh</creatorcontrib><creatorcontrib>Soleimani, Masoud</creatorcontrib><creatorcontrib>Dehghanifard, Ali</creatorcontrib><creatorcontrib>Atwan, Hossein</creatorcontrib><creatorcontrib>Ajami, Monireh</creatorcontrib><creatorcontrib>Ajami, Mansoureh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tayebi, Behnoosh</au><au>Abrishami, Fatemeh</au><au>Alizadeh, Shaban</au><au>Minayi, Neda</au><au>Mohammadian, Mozhdeh</au><au>Soleimani, Masoud</au><au>Dehghanifard, Ali</au><au>Atwan, Hossein</au><au>Ajami, Monireh</au><au>Ajami, Mansoureh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of microRNAs expression in hematopoietic stem cells treated with sodium butyrate in inducing fetal hemoglobin expression</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><addtitle>Artif Cells Nanomed Biotechnol</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>45</volume><issue>1</issue><spage>146</spage><epage>156</epage><pages>146-156</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>Context Inherited hemoglobin diseases are the most common single-gene disorders. Induction of fetal hemoglobin in beta hemoglobin disorders compensate for abnormal chain and ameliorate the clinical complications. Sodium butyrate is used conventionally for fetal hemoglobin induction; it can be replaced by safer therapeutic tools like microRNAs, small non-coding RNAs that control number of epigenetic mechanisms. Objective In this study, we compared the changes in the microRNAs of differentiated erythroid cells between control and sodium butyrate treated groups. The objective is to find significant association between these changes and gamma chain up regulation. Materials and methods First, CD133
+
hematopoietic stem cells were isolated from cord blood by magnetic cell sorting (MACS) technique. After proliferation, the cells were differentiated to erythroid lineage in culture medium by EPO, SCF, and IL3. Meanwhile, the test group was treated with sodium butyrate. Then, gamma chain upregulation was verified by qPCR technique. Finally, microRNA profiling was performed through microarray assay and some of them confirmed by qPCR. Result Results demonstrated that gamma chain was 5.9-fold upregulated in the treated group. Significant changes were observed at 76 microRNAs, in which 20 were up-regulated and 56 were down-regulated. Discussion Five of these microRNAs including U101, hsa-miR-4726-5p, hsa-miR7109 5p, hsa-miR3663, and hsa-miR940 had significant changes in expression and volume. Conclusion In conclusion, it can be assumed that sodium butyrate can up-regulate gamma chain gene, and change miRNAs expression. These results can be profitable in future studies to find therapeutic goal suitable for such disorders.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>26837891</pmid><doi>10.3109/21691401.2016.1138487</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Butyric Acid - pharmacology Cell culture Cell differentiation Cell proliferation Cells, Cultured Chains Cord blood Disorders DNA microarrays Epigenetics Erythroid cells Fetal hemoglobin Fetal Hemoglobin - biosynthesis Fetal Hemoglobin - genetics Fetuses Gene expression Gene Expression Regulation - drug effects Genes hematopoietic stem cell Hematopoietic stem cells Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - metabolism Hemoglobin Humans Interleukin 3 microarray microRNA MicroRNAs MicroRNAs - biosynthesis MicroRNAs - genetics miRNA Ribonucleic acid RNA sickle cell anemia Sodium Sodium butyrate Stem cells thalassemia |
| title | Modulation of microRNAs expression in hematopoietic stem cells treated with sodium butyrate in inducing fetal hemoglobin expression |
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