A novel glutamine biosensor based on zinc oxide nanorod and glutaminase enzyme from Hypocria jecorina
A novel biosensor for determination of L-glutamine in pharmaceutical glutamine powder was developed via immobilizing our produced glutaminase enzyme from Hypocria jecorina onto our prepared zinc oxide (ZnO) nanorod and chitosan. ZnO nanorods were prepared as surface-dependent and surface-independent...
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| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2016-01, Vol.44 (1), p.92-97 |
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| container_title | Artificial cells, nanomedicine, and biotechnology |
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| creator | Albayrak, Dilruba Karakuş, Emine |
| description | A novel biosensor for determination of L-glutamine in pharmaceutical glutamine powder was developed via immobilizing our produced glutaminase enzyme from Hypocria jecorina onto our prepared zinc oxide (ZnO) nanorod and chitosan. ZnO nanorods were prepared as surface-dependent and surface-independent and both were used. The biosensor is specific for L-glutamine and the peculiar analytical properties (linearity range, reproducibility, and accuracy) of it were experimentally determined. The optimum operating conditions of the biosensor such as buffer concentration, buffer pH, and medium temperature effect on the response of biosensor were studied. K
m
and V
max
values for the our-producing glutaminase enzyme from Hypocria jecorina immobilized on the biosensor were also determined as 0.29 mM and 208.33 mV/min., respectively, from Lineweaver-Burk plot. The biosensor was then used for the determination of glutamine contained in pharmaceutical formulations. |
| doi_str_mv | 10.3109/21691401.2014.913055 |
| format | Article |
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m
and V
max
values for the our-producing glutaminase enzyme from Hypocria jecorina immobilized on the biosensor were also determined as 0.29 mM and 208.33 mV/min., respectively, from Lineweaver-Burk plot. The biosensor was then used for the determination of glutamine contained in pharmaceutical formulations.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.3109/21691401.2014.913055</identifier><identifier>PMID: 24848995</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Biosensing Techniques ; biosensor ; Biosensors ; Buffers ; Chitosan ; Chitosan - chemistry ; Electrochemical Techniques ; Enzymes ; Enzymes, Immobilized ; Formulations ; Fungal Proteins - chemistry ; Glutaminase ; Glutaminase - chemistry ; Glutamine ; Glutamine - analysis ; Hydrogen-Ion Concentration ; Linearity ; metal oxide nanorod ; nanorod ; Nanorods ; Nanotubes - chemistry ; Nanotubes - ultrastructure ; pH effects ; Pharmaceuticals ; Powder ; Powders ; Reproducibility ; Reproducibility of Results ; Sensitivity and Specificity ; Temperature effects ; Trichoderma - chemistry ; Trichoderma - enzymology ; Zinc oxide ; Zinc Oxide - chemistry ; Zinc oxides</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2016-01, Vol.44 (1), p.92-97</ispartof><rights>2014 Informa Healthcare USA, Inc. 2014</rights><rights>2014 Informa Healthcare USA, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-5c6acfc9c0b45635d2206e64816bd7493a4b23f967dec2ab338224238485437d3</citedby><cites>FETCH-LOGICAL-c391t-5c6acfc9c0b45635d2206e64816bd7493a4b23f967dec2ab338224238485437d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24848995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Albayrak, Dilruba</creatorcontrib><creatorcontrib>Karakuş, Emine</creatorcontrib><title>A novel glutamine biosensor based on zinc oxide nanorod and glutaminase enzyme from Hypocria jecorina</title><title>Artificial cells, nanomedicine, and biotechnology</title><addtitle>Artif Cells Nanomed Biotechnol</addtitle><description>A novel biosensor for determination of L-glutamine in pharmaceutical glutamine powder was developed via immobilizing our produced glutaminase enzyme from Hypocria jecorina onto our prepared zinc oxide (ZnO) nanorod and chitosan. ZnO nanorods were prepared as surface-dependent and surface-independent and both were used. The biosensor is specific for L-glutamine and the peculiar analytical properties (linearity range, reproducibility, and accuracy) of it were experimentally determined. The optimum operating conditions of the biosensor such as buffer concentration, buffer pH, and medium temperature effect on the response of biosensor were studied. K
m
and V
max
values for the our-producing glutaminase enzyme from Hypocria jecorina immobilized on the biosensor were also determined as 0.29 mM and 208.33 mV/min., respectively, from Lineweaver-Burk plot. The biosensor was then used for the determination of glutamine contained in pharmaceutical formulations.</description><subject>Biosensing Techniques</subject><subject>biosensor</subject><subject>Biosensors</subject><subject>Buffers</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Electrochemical Techniques</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized</subject><subject>Formulations</subject><subject>Fungal Proteins - chemistry</subject><subject>Glutaminase</subject><subject>Glutaminase - chemistry</subject><subject>Glutamine</subject><subject>Glutamine - analysis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Linearity</subject><subject>metal oxide nanorod</subject><subject>nanorod</subject><subject>Nanorods</subject><subject>Nanotubes - chemistry</subject><subject>Nanotubes - ultrastructure</subject><subject>pH effects</subject><subject>Pharmaceuticals</subject><subject>Powder</subject><subject>Powders</subject><subject>Reproducibility</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Temperature effects</subject><subject>Trichoderma - chemistry</subject><subject>Trichoderma - enzymology</subject><subject>Zinc oxide</subject><subject>Zinc Oxide - chemistry</subject><subject>Zinc oxides</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9rVDEUxYMottR-A5GAGzcz5ubfe1lJKWoLBTcK7kJecp9keC8Zk3na6ac3w7SzcNFsEnJ_5-TeHELeAlsLYOYjB21AMlhzBnJtQDClXpDzw_UKJPx8eTozOCOXtW5YWz3oTsnX5IzLXvbGqHOCVzTlPzjRX9Oyc3NMSIeYK6aaCx1cxUBzog8xeZrvY0CaXMolB-pSOGkaRjE97GekY8kzvdlvsy_R0Q36XFr9DXk1uqni5eN-QX58-fz9-mZ19-3r7fXV3coLA7uV8tr50RvPBqm0UIFzplHL1vcQOmmEkwMXo9FdQM_dIETPueSiDaOk6IK4IB-OvtuSfy9Yd3aO1eM0uYR5qRY6zXotOO8b-v4_dJOXklp3Fgz0GpgC1Sh5pHzJtRYc7bbE2ZW9BWYPSdinJOwhCXtMosnePZovw4zhJHr69wZ8OgIxjbnM7m8uU7A7t59yGYtLPtaD_TNP_AOCOJZ-</recordid><startdate>20160102</startdate><enddate>20160102</enddate><creator>Albayrak, Dilruba</creator><creator>Karakuş, Emine</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160102</creationdate><title>A novel glutamine biosensor based on zinc oxide nanorod and glutaminase enzyme from Hypocria jecorina</title><author>Albayrak, Dilruba ; Karakuş, Emine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-5c6acfc9c0b45635d2206e64816bd7493a4b23f967dec2ab338224238485437d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biosensing Techniques</topic><topic>biosensor</topic><topic>Biosensors</topic><topic>Buffers</topic><topic>Chitosan</topic><topic>Chitosan - chemistry</topic><topic>Electrochemical Techniques</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized</topic><topic>Formulations</topic><topic>Fungal Proteins - chemistry</topic><topic>Glutaminase</topic><topic>Glutaminase - chemistry</topic><topic>Glutamine</topic><topic>Glutamine - analysis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Linearity</topic><topic>metal oxide nanorod</topic><topic>nanorod</topic><topic>Nanorods</topic><topic>Nanotubes - chemistry</topic><topic>Nanotubes - ultrastructure</topic><topic>pH effects</topic><topic>Pharmaceuticals</topic><topic>Powder</topic><topic>Powders</topic><topic>Reproducibility</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Temperature effects</topic><topic>Trichoderma - chemistry</topic><topic>Trichoderma - enzymology</topic><topic>Zinc oxide</topic><topic>Zinc Oxide - chemistry</topic><topic>Zinc oxides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Albayrak, Dilruba</creatorcontrib><creatorcontrib>Karakuş, Emine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Albayrak, Dilruba</au><au>Karakuş, Emine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel glutamine biosensor based on zinc oxide nanorod and glutaminase enzyme from Hypocria jecorina</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><addtitle>Artif Cells Nanomed Biotechnol</addtitle><date>2016-01-02</date><risdate>2016</risdate><volume>44</volume><issue>1</issue><spage>92</spage><epage>97</epage><pages>92-97</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>A novel biosensor for determination of L-glutamine in pharmaceutical glutamine powder was developed via immobilizing our produced glutaminase enzyme from Hypocria jecorina onto our prepared zinc oxide (ZnO) nanorod and chitosan. ZnO nanorods were prepared as surface-dependent and surface-independent and both were used. The biosensor is specific for L-glutamine and the peculiar analytical properties (linearity range, reproducibility, and accuracy) of it were experimentally determined. The optimum operating conditions of the biosensor such as buffer concentration, buffer pH, and medium temperature effect on the response of biosensor were studied. K
m
and V
max
values for the our-producing glutaminase enzyme from Hypocria jecorina immobilized on the biosensor were also determined as 0.29 mM and 208.33 mV/min., respectively, from Lineweaver-Burk plot. The biosensor was then used for the determination of glutamine contained in pharmaceutical formulations.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>24848995</pmid><doi>10.3109/21691401.2014.913055</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2169-1401 |
| ispartof | Artificial cells, nanomedicine, and biotechnology, 2016-01, Vol.44 (1), p.92-97 |
| issn | 2169-1401 2169-141X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Biosensing Techniques biosensor Biosensors Buffers Chitosan Chitosan - chemistry Electrochemical Techniques Enzymes Enzymes, Immobilized Formulations Fungal Proteins - chemistry Glutaminase Glutaminase - chemistry Glutamine Glutamine - analysis Hydrogen-Ion Concentration Linearity metal oxide nanorod nanorod Nanorods Nanotubes - chemistry Nanotubes - ultrastructure pH effects Pharmaceuticals Powder Powders Reproducibility Reproducibility of Results Sensitivity and Specificity Temperature effects Trichoderma - chemistry Trichoderma - enzymology Zinc oxide Zinc Oxide - chemistry Zinc oxides |
| title | A novel glutamine biosensor based on zinc oxide nanorod and glutaminase enzyme from Hypocria jecorina |
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