Inhibitory effect of Terminalia chebula Retz. fruit extracts on digestive enzyme related to diabetes and oxidative stress
Terminalia chebula fruit extracts were prepared sequentially with hexane, ethyl acetate, methanol and methanol-water (70:30) and tested for their α-glucosidase inhibitory and antioxidant potential. The study resulted in the formulation of an extract with high α-glucosidase inhibitory potential (IC50...
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| Veröffentlicht in: | Journal of enzyme inhibition and medicinal chemistry 2012-08, Vol.27 (4), p.578-586 |
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| container_title | Journal of enzyme inhibition and medicinal chemistry |
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| creator | Sasidharan, Indu Sundaresan, A. Nisha, V. M. Kirishna, Mahesh S. Raghu, K. G. Jayamurthy, P. |
| description | Terminalia chebula fruit extracts were prepared sequentially with hexane, ethyl acetate, methanol and methanol-water (70:30) and tested for their α-glucosidase inhibitory and antioxidant potential. The study resulted in the formulation of an extract with high α-glucosidase inhibitory potential (IC50 0.19 ± 0.03 µg mL−1) enriched with hydrolysable tannins. Also, each of the extract was chemically characterized by reversed-phase high-performance liquid chromatography on the basis of their marker compounds chebulagic acid, chebulinic acid and corilagin in order to give explanation to the significant activity shown by the extracts. The antioxidant potential of the highly active extract was evaluated in the cellular level also using superoxide dismutase, glutathione S-transferase and induced oxidative stress assays. The results indicated the possibility of using the extract as a nutraceutical health supplement in the management of type 2 diabetes. |
| doi_str_mv | 10.3109/14756366.2011.603130 |
| format | Article |
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The antioxidant potential of the highly active extract was evaluated in the cellular level also using superoxide dismutase, glutathione S-transferase and induced oxidative stress assays. The results indicated the possibility of using the extract as a nutraceutical health supplement in the management of type 2 diabetes.</description><identifier>ISSN: 1475-6366</identifier><identifier>EISSN: 1475-6374</identifier><identifier>DOI: 10.3109/14756366.2011.603130</identifier><identifier>PMID: 22512724</identifier><language>eng</language><publisher>England: Informa Healthcare</publisher><subject>alpha-Glucosidases - metabolism ; Animals ; Antioxidants - chemistry ; Antioxidants - isolation & purification ; Antioxidants - pharmacology ; Cells, Cultured ; chebulinic acid ; Chromatography, High Pressure Liquid ; Diabetes Mellitus, Type 2 - enzymology ; Diabetes Mellitus, Type 2 - metabolism ; Dose-Response Relationship, Drug ; Enzyme Inhibitors - chemistry ; Enzyme Inhibitors - isolation & purification ; Enzyme Inhibitors - pharmacology ; flow cytometry ; Fruit - chemistry ; Glutathione Transferase - metabolism ; Glycoside Hydrolase Inhibitors ; HepG2 cell line ; HPLC ; Mice ; Oxidative Stress - drug effects ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Reactive Oxygen Species - metabolism ; Structure-Activity Relationship ; Superoxide Dismutase - metabolism ; Terminalia - chemistry ; α-glucosidase inhibition</subject><ispartof>Journal of enzyme inhibition and medicinal chemistry, 2012-08, Vol.27 (4), p.578-586</ispartof><rights>2012 Informa UK, Ltd. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-86c565766c18f27c0ebd8c438c00343428ca7cc43ea3cd42f5e787ea862376a43</citedby><cites>FETCH-LOGICAL-c418t-86c565766c18f27c0ebd8c438c00343428ca7cc43ea3cd42f5e787ea862376a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22512724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sasidharan, Indu</creatorcontrib><creatorcontrib>Sundaresan, A.</creatorcontrib><creatorcontrib>Nisha, V. M.</creatorcontrib><creatorcontrib>Kirishna, Mahesh S.</creatorcontrib><creatorcontrib>Raghu, K. G.</creatorcontrib><creatorcontrib>Jayamurthy, P.</creatorcontrib><title>Inhibitory effect of Terminalia chebula Retz. fruit extracts on digestive enzyme related to diabetes and oxidative stress</title><title>Journal of enzyme inhibition and medicinal chemistry</title><addtitle>J Enzyme Inhib Med Chem</addtitle><description>Terminalia chebula fruit extracts were prepared sequentially with hexane, ethyl acetate, methanol and methanol-water (70:30) and tested for their α-glucosidase inhibitory and antioxidant potential. The study resulted in the formulation of an extract with high α-glucosidase inhibitory potential (IC50 0.19 ± 0.03 µg mL−1) enriched with hydrolysable tannins. Also, each of the extract was chemically characterized by reversed-phase high-performance liquid chromatography on the basis of their marker compounds chebulagic acid, chebulinic acid and corilagin in order to give explanation to the significant activity shown by the extracts. The antioxidant potential of the highly active extract was evaluated in the cellular level also using superoxide dismutase, glutathione S-transferase and induced oxidative stress assays. The results indicated the possibility of using the extract as a nutraceutical health supplement in the management of type 2 diabetes.</description><subject>alpha-Glucosidases - metabolism</subject><subject>Animals</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - pharmacology</subject><subject>Cells, Cultured</subject><subject>chebulinic acid</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Diabetes Mellitus, Type 2 - enzymology</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Inhibitors - chemistry</subject><subject>Enzyme Inhibitors - isolation & purification</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>flow cytometry</subject><subject>Fruit - chemistry</subject><subject>Glutathione Transferase - metabolism</subject><subject>Glycoside Hydrolase Inhibitors</subject><subject>HepG2 cell line</subject><subject>HPLC</subject><subject>Mice</subject><subject>Oxidative Stress - drug effects</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Structure-Activity Relationship</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Terminalia - chemistry</subject><subject>α-glucosidase inhibition</subject><issn>1475-6366</issn><issn>1475-6374</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1r3DAQhkVpadJN_0EpOvayG31ZUi8tJfQjEAiU5GzG8qirIFupJKdxfn283SSQS04So2feGR4R8oGzjeTs8zFXptFS641gnG80k1yyV-RwV15radTrp7vWB-RdKVeMCS64eksOhGi4MEIdkvl03IYu1JRnit6jqzR5eoF5CCPEANRtsZsi0N9Y7zbU5ylUirc1g6uFppH24Q-WGm6Q4ng3D0gzRqjY05qWN-iwYqEw9jTdhh7-g6VmLOWIvPEQC75_OFfk8sf3i5Nf67Pzn6cn387WTnFb11a7RjdGa8etF8Yx7HrrlLSOMamkEtaBcUsBQbpeCd-gsQbBaiGNBiVX5NM-9zqnv9OyazuE4jBGGDFNpeVMGGmkXfytiNqjLqdSMvr2OocB8rxA7U56-yi93Ulv99KXto8PE6ZuwP6p6dHyAnzdA2H0KQ_wL-XYtxXmmLLPMLpQdvEvjvjyLGGLEOvWQcb2Kk15-ary8o73o4-lcA</recordid><startdate>201208</startdate><enddate>201208</enddate><creator>Sasidharan, Indu</creator><creator>Sundaresan, A.</creator><creator>Nisha, V. M.</creator><creator>Kirishna, Mahesh S.</creator><creator>Raghu, K. G.</creator><creator>Jayamurthy, P.</creator><general>Informa Healthcare</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201208</creationdate><title>Inhibitory effect of Terminalia chebula Retz. fruit extracts on digestive enzyme related to diabetes and oxidative stress</title><author>Sasidharan, Indu ; Sundaresan, A. ; Nisha, V. M. ; Kirishna, Mahesh S. ; Raghu, K. G. ; Jayamurthy, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-86c565766c18f27c0ebd8c438c00343428ca7cc43ea3cd42f5e787ea862376a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>alpha-Glucosidases - metabolism</topic><topic>Animals</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - isolation & purification</topic><topic>Antioxidants - pharmacology</topic><topic>Cells, Cultured</topic><topic>chebulinic acid</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Diabetes Mellitus, Type 2 - enzymology</topic><topic>Diabetes Mellitus, Type 2 - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Inhibitors - chemistry</topic><topic>Enzyme Inhibitors - isolation & purification</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>flow cytometry</topic><topic>Fruit - chemistry</topic><topic>Glutathione Transferase - metabolism</topic><topic>Glycoside Hydrolase Inhibitors</topic><topic>HepG2 cell line</topic><topic>HPLC</topic><topic>Mice</topic><topic>Oxidative Stress - drug effects</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Structure-Activity Relationship</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Terminalia - chemistry</topic><topic>α-glucosidase inhibition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sasidharan, Indu</creatorcontrib><creatorcontrib>Sundaresan, A.</creatorcontrib><creatorcontrib>Nisha, V. 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G.</au><au>Jayamurthy, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of Terminalia chebula Retz. fruit extracts on digestive enzyme related to diabetes and oxidative stress</atitle><jtitle>Journal of enzyme inhibition and medicinal chemistry</jtitle><addtitle>J Enzyme Inhib Med Chem</addtitle><date>2012-08</date><risdate>2012</risdate><volume>27</volume><issue>4</issue><spage>578</spage><epage>586</epage><pages>578-586</pages><issn>1475-6366</issn><eissn>1475-6374</eissn><abstract>Terminalia chebula fruit extracts were prepared sequentially with hexane, ethyl acetate, methanol and methanol-water (70:30) and tested for their α-glucosidase inhibitory and antioxidant potential. The study resulted in the formulation of an extract with high α-glucosidase inhibitory potential (IC50 0.19 ± 0.03 µg mL−1) enriched with hydrolysable tannins. Also, each of the extract was chemically characterized by reversed-phase high-performance liquid chromatography on the basis of their marker compounds chebulagic acid, chebulinic acid and corilagin in order to give explanation to the significant activity shown by the extracts. The antioxidant potential of the highly active extract was evaluated in the cellular level also using superoxide dismutase, glutathione S-transferase and induced oxidative stress assays. The results indicated the possibility of using the extract as a nutraceutical health supplement in the management of type 2 diabetes.</abstract><cop>England</cop><pub>Informa Healthcare</pub><pmid>22512724</pmid><doi>10.3109/14756366.2011.603130</doi><tpages>9</tpages></addata></record> |
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| subjects | alpha-Glucosidases - metabolism Animals Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - pharmacology Cells, Cultured chebulinic acid Chromatography, High Pressure Liquid Diabetes Mellitus, Type 2 - enzymology Diabetes Mellitus, Type 2 - metabolism Dose-Response Relationship, Drug Enzyme Inhibitors - chemistry Enzyme Inhibitors - isolation & purification Enzyme Inhibitors - pharmacology flow cytometry Fruit - chemistry Glutathione Transferase - metabolism Glycoside Hydrolase Inhibitors HepG2 cell line HPLC Mice Oxidative Stress - drug effects Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Reactive Oxygen Species - metabolism Structure-Activity Relationship Superoxide Dismutase - metabolism Terminalia - chemistry α-glucosidase inhibition |
| title | Inhibitory effect of Terminalia chebula Retz. fruit extracts on digestive enzyme related to diabetes and oxidative stress |
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