Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: In vivo and in vitro studies
Context: Dracocephalum polychaetum Bornm. (Lamiaceae) is used in folk medicine and contains antioxidant agents. Objectives: The objective of this study is to investigate the antidiabetic, antioxidant, and antilipid peroxidative properties of methanol extract of D. polychaetum aerial parts. Materials...
Gespeichert in:
| Veröffentlicht in: | Pharmaceutical biology 2016-02, Vol.54 (2), p.272-278 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 278 |
|---|---|
| container_issue | 2 |
| container_start_page | 272 |
| container_title | Pharmaceutical biology |
| container_volume | 54 |
| creator | Pouraboli, Iran Nazari, Somaye Sabet, Nazanin Sharififar, Fariba Jafari, Mahvash |
| description | Context: Dracocephalum polychaetum Bornm. (Lamiaceae) is used in folk medicine and contains antioxidant agents.
Objectives: The objective of this study is to investigate the antidiabetic, antioxidant, and antilipid peroxidative properties of methanol extract of D. polychaetum aerial parts.
Materials and methods: The effect of extract (200, 300, and 400 mg/kg, b.w.) on oral glucose tolerance test (OGTT) was investigated. Also, extract (300 mg/kg) administered orally in diabetic rats for 14 d then, serum levels of some biochemical factors were evaluated. Pancreas samples were used for the determination of malondealdehyde (MDA) level, glutathione (GSH) content, superoxide dismutase, and catalase enzyme activities. Red blood cells (RBCs) and plasma were used for MDA estimation. Pancreatic α-amylase inhibition, DPPH (1,1-diphenyl-2-picrylhydrazyl), and FRAP (ferric reducing antioxidant power) assays were done. The total flavonoid content of the extract was determined by spectrophotometry.
Results: Extract (300 mg/kg) decreased serum glucose level (27.1%) significantly at 120 min in OGTT. Serum levels of creatinine, triglycerides, cholesterol, alanine amino transferase and MDA levels in plasma, RBCs, and pancreas significantly decreased in treated (300 mg/kg) diabetic rats, while pancreatic GSH content, superoxide dismutase, and catalase enzymatic activities increased (p |
| doi_str_mv | 10.3109/13880209.2015.1033561 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_3109_13880209_2015_1033561</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1747328720</sourcerecordid><originalsourceid>FETCH-LOGICAL-c366t-1864189ddde156b6a7a71b8f9109d25a828bd1ddcd6762fbd6fd7dd4f230063</originalsourceid><addsrcrecordid>eNp9kctu1TAQhiMEohd4BJCXLMjBlxMnYUXVFqhUiQXsLcdj6wxK4mA7hx7eqm9Y51xYdvXP2N9cNH9RvGN0JRhtPzHRNJTTdsUpq1aMClFJ9qI4Z_V6XVaMyZc5zky5QGfFRYy_KaVVxl4XZ7xqKasFOy8er8aEgLqzCc1HonPmHxCyLgnsH3qcEMhkw_4n4dYSbbJgQhuJd-QmaOONnTa6nwcy-X5nNtqmHMeN94nYh5SJRHAkMQU7Jf_PJ29wLHGE2Vggpw1I0Cl-Jncj2eLW7zfAJU7B59IZ8sA3xSun-2jfHvWy-Pn19tf19_L-x7e766v70ggpU8kauWZNCwCWVbKTutY16xrX5uMBr3TDmw4YgAFZS-46kA5qgLXjglIpLosPh65T8H9mG5MaMBrb93q0fo4qn7kWvKk5zWh1QE3wMQbr1BRw0GGnGFWLV-rklVq8Ukevct3744i5Gyz8rzqZk4EvBwBH58Og__rQg0p61_vggh4NxqX_czOeAIm0qJY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1747328720</pqid></control><display><type>article</type><title>Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: In vivo and in vitro studies</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Pouraboli, Iran ; Nazari, Somaye ; Sabet, Nazanin ; Sharififar, Fariba ; Jafari, Mahvash</creator><creatorcontrib>Pouraboli, Iran ; Nazari, Somaye ; Sabet, Nazanin ; Sharififar, Fariba ; Jafari, Mahvash</creatorcontrib><description>Context: Dracocephalum polychaetum Bornm. (Lamiaceae) is used in folk medicine and contains antioxidant agents.
Objectives: The objective of this study is to investigate the antidiabetic, antioxidant, and antilipid peroxidative properties of methanol extract of D. polychaetum aerial parts.
Materials and methods: The effect of extract (200, 300, and 400 mg/kg, b.w.) on oral glucose tolerance test (OGTT) was investigated. Also, extract (300 mg/kg) administered orally in diabetic rats for 14 d then, serum levels of some biochemical factors were evaluated. Pancreas samples were used for the determination of malondealdehyde (MDA) level, glutathione (GSH) content, superoxide dismutase, and catalase enzyme activities. Red blood cells (RBCs) and plasma were used for MDA estimation. Pancreatic α-amylase inhibition, DPPH (1,1-diphenyl-2-picrylhydrazyl), and FRAP (ferric reducing antioxidant power) assays were done. The total flavonoid content of the extract was determined by spectrophotometry.
Results: Extract (300 mg/kg) decreased serum glucose level (27.1%) significantly at 120 min in OGTT. Serum levels of creatinine, triglycerides, cholesterol, alanine amino transferase and MDA levels in plasma, RBCs, and pancreas significantly decreased in treated (300 mg/kg) diabetic rats, while pancreatic GSH content, superoxide dismutase, and catalase enzymatic activities increased (p < 0.05). The IC
50
values for the extract and butyl hydroxyanisole were 5.6 and 1.15 mg/mL in DPPH and 0.155 and 0.062 mg/mL in the FRAP methods, respectively. The extract had no inhibitory effect on α-amylase activity. The total amount of flavonoids of the extract was estimated to be 1.8% (g/g) on the basis of quercetin content.
Conclusion: Dracocephalum polychaetum shoot extract has antioxidant, antihyperlipidemic, and antilipid peroxidative properties.</description><identifier>ISSN: 1388-0209</identifier><identifier>EISSN: 1744-5116</identifier><identifier>DOI: 10.3109/13880209.2015.1033561</identifier><identifier>PMID: 25901731</identifier><language>eng</language><publisher>England: Informa Healthcare</publisher><subject><![CDATA[Administration, Oral ; Alpha-amylase inhibitory activity ; Animals ; Antioxidants - administration & dosage ; Antioxidants - isolation & purification ; Antioxidants - therapeutic use ; Biomarkers - blood ; Biphenyl Compounds - chemistry ; Blood Glucose - analysis ; diabetes mellitus ; Diabetes Mellitus, Experimental - blood ; Diabetes Mellitus, Experimental - drug therapy ; Diabetes Mellitus, Experimental - metabolism ; Dose-Response Relationship, Drug ; Glucose Tolerance Test ; Hypoglycemic Agents - administration & dosage ; Hypoglycemic Agents - isolation & purification ; Hypoglycemic Agents - therapeutic use ; Hypolipidemic Agents - administration & dosage ; Hypolipidemic Agents - isolation & purification ; Hypolipidemic Agents - therapeutic use ; Lamiaceae - chemistry ; Lipid Peroxidation - drug effects ; Male ; Malondialdehyde - blood ; MDA ; medicinal plants ; pancreas ; Picrates - chemistry ; Plant Extracts - administration & dosage ; Plant Extracts - isolation & purification ; Plant Extracts - therapeutic use ; Plant Shoots - chemistry ; Rats, Wistar ; Streptozocin]]></subject><ispartof>Pharmaceutical biology, 2016-02, Vol.54 (2), p.272-278</ispartof><rights>2015 Informa Healthcare USA, Inc. 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-1864189ddde156b6a7a71b8f9109d25a828bd1ddcd6762fbd6fd7dd4f230063</citedby><cites>FETCH-LOGICAL-c366t-1864189ddde156b6a7a71b8f9109d25a828bd1ddcd6762fbd6fd7dd4f230063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25901731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pouraboli, Iran</creatorcontrib><creatorcontrib>Nazari, Somaye</creatorcontrib><creatorcontrib>Sabet, Nazanin</creatorcontrib><creatorcontrib>Sharififar, Fariba</creatorcontrib><creatorcontrib>Jafari, Mahvash</creatorcontrib><title>Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: In vivo and in vitro studies</title><title>Pharmaceutical biology</title><addtitle>Pharm Biol</addtitle><description>Context: Dracocephalum polychaetum Bornm. (Lamiaceae) is used in folk medicine and contains antioxidant agents.
Objectives: The objective of this study is to investigate the antidiabetic, antioxidant, and antilipid peroxidative properties of methanol extract of D. polychaetum aerial parts.
Materials and methods: The effect of extract (200, 300, and 400 mg/kg, b.w.) on oral glucose tolerance test (OGTT) was investigated. Also, extract (300 mg/kg) administered orally in diabetic rats for 14 d then, serum levels of some biochemical factors were evaluated. Pancreas samples were used for the determination of malondealdehyde (MDA) level, glutathione (GSH) content, superoxide dismutase, and catalase enzyme activities. Red blood cells (RBCs) and plasma were used for MDA estimation. Pancreatic α-amylase inhibition, DPPH (1,1-diphenyl-2-picrylhydrazyl), and FRAP (ferric reducing antioxidant power) assays were done. The total flavonoid content of the extract was determined by spectrophotometry.
Results: Extract (300 mg/kg) decreased serum glucose level (27.1%) significantly at 120 min in OGTT. Serum levels of creatinine, triglycerides, cholesterol, alanine amino transferase and MDA levels in plasma, RBCs, and pancreas significantly decreased in treated (300 mg/kg) diabetic rats, while pancreatic GSH content, superoxide dismutase, and catalase enzymatic activities increased (p < 0.05). The IC
50
values for the extract and butyl hydroxyanisole were 5.6 and 1.15 mg/mL in DPPH and 0.155 and 0.062 mg/mL in the FRAP methods, respectively. The extract had no inhibitory effect on α-amylase activity. The total amount of flavonoids of the extract was estimated to be 1.8% (g/g) on the basis of quercetin content.
Conclusion: Dracocephalum polychaetum shoot extract has antioxidant, antihyperlipidemic, and antilipid peroxidative properties.</description><subject>Administration, Oral</subject><subject>Alpha-amylase inhibitory activity</subject><subject>Animals</subject><subject>Antioxidants - administration & dosage</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - therapeutic use</subject><subject>Biomarkers - blood</subject><subject>Biphenyl Compounds - chemistry</subject><subject>Blood Glucose - analysis</subject><subject>diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - blood</subject><subject>Diabetes Mellitus, Experimental - drug therapy</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Glucose Tolerance Test</subject><subject>Hypoglycemic Agents - administration & dosage</subject><subject>Hypoglycemic Agents - isolation & purification</subject><subject>Hypoglycemic Agents - therapeutic use</subject><subject>Hypolipidemic Agents - administration & dosage</subject><subject>Hypolipidemic Agents - isolation & purification</subject><subject>Hypolipidemic Agents - therapeutic use</subject><subject>Lamiaceae - chemistry</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>MDA</subject><subject>medicinal plants</subject><subject>pancreas</subject><subject>Picrates - chemistry</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - therapeutic use</subject><subject>Plant Shoots - chemistry</subject><subject>Rats, Wistar</subject><subject>Streptozocin</subject><issn>1388-0209</issn><issn>1744-5116</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1TAQhiMEohd4BJCXLMjBlxMnYUXVFqhUiQXsLcdj6wxK4mA7hx7eqm9Y51xYdvXP2N9cNH9RvGN0JRhtPzHRNJTTdsUpq1aMClFJ9qI4Z_V6XVaMyZc5zky5QGfFRYy_KaVVxl4XZ7xqKasFOy8er8aEgLqzCc1HonPmHxCyLgnsH3qcEMhkw_4n4dYSbbJgQhuJd-QmaOONnTa6nwcy-X5nNtqmHMeN94nYh5SJRHAkMQU7Jf_PJ29wLHGE2Vggpw1I0Cl-Jncj2eLW7zfAJU7B59IZ8sA3xSun-2jfHvWy-Pn19tf19_L-x7e766v70ggpU8kauWZNCwCWVbKTutY16xrX5uMBr3TDmw4YgAFZS-46kA5qgLXjglIpLosPh65T8H9mG5MaMBrb93q0fo4qn7kWvKk5zWh1QE3wMQbr1BRw0GGnGFWLV-rklVq8Ukevct3744i5Gyz8rzqZk4EvBwBH58Og__rQg0p61_vggh4NxqX_czOeAIm0qJY</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>Pouraboli, Iran</creator><creator>Nazari, Somaye</creator><creator>Sabet, Nazanin</creator><creator>Sharififar, Fariba</creator><creator>Jafari, Mahvash</creator><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20160201</creationdate><title>Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: In vivo and in vitro studies</title><author>Pouraboli, Iran ; Nazari, Somaye ; Sabet, Nazanin ; Sharififar, Fariba ; Jafari, Mahvash</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-1864189ddde156b6a7a71b8f9109d25a828bd1ddcd6762fbd6fd7dd4f230063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Administration, Oral</topic><topic>Alpha-amylase inhibitory activity</topic><topic>Animals</topic><topic>Antioxidants - administration & dosage</topic><topic>Antioxidants - isolation & purification</topic><topic>Antioxidants - therapeutic use</topic><topic>Biomarkers - blood</topic><topic>Biphenyl Compounds - chemistry</topic><topic>Blood Glucose - analysis</topic><topic>diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - blood</topic><topic>Diabetes Mellitus, Experimental - drug therapy</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Glucose Tolerance Test</topic><topic>Hypoglycemic Agents - administration & dosage</topic><topic>Hypoglycemic Agents - isolation & purification</topic><topic>Hypoglycemic Agents - therapeutic use</topic><topic>Hypolipidemic Agents - administration & dosage</topic><topic>Hypolipidemic Agents - isolation & purification</topic><topic>Hypolipidemic Agents - therapeutic use</topic><topic>Lamiaceae - chemistry</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>Malondialdehyde - blood</topic><topic>MDA</topic><topic>medicinal plants</topic><topic>pancreas</topic><topic>Picrates - chemistry</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - therapeutic use</topic><topic>Plant Shoots - chemistry</topic><topic>Rats, Wistar</topic><topic>Streptozocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pouraboli, Iran</creatorcontrib><creatorcontrib>Nazari, Somaye</creatorcontrib><creatorcontrib>Sabet, Nazanin</creatorcontrib><creatorcontrib>Sharififar, Fariba</creatorcontrib><creatorcontrib>Jafari, Mahvash</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmaceutical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pouraboli, Iran</au><au>Nazari, Somaye</au><au>Sabet, Nazanin</au><au>Sharififar, Fariba</au><au>Jafari, Mahvash</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: In vivo and in vitro studies</atitle><jtitle>Pharmaceutical biology</jtitle><addtitle>Pharm Biol</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>54</volume><issue>2</issue><spage>272</spage><epage>278</epage><pages>272-278</pages><issn>1388-0209</issn><eissn>1744-5116</eissn><abstract>Context: Dracocephalum polychaetum Bornm. (Lamiaceae) is used in folk medicine and contains antioxidant agents.
Objectives: The objective of this study is to investigate the antidiabetic, antioxidant, and antilipid peroxidative properties of methanol extract of D. polychaetum aerial parts.
Materials and methods: The effect of extract (200, 300, and 400 mg/kg, b.w.) on oral glucose tolerance test (OGTT) was investigated. Also, extract (300 mg/kg) administered orally in diabetic rats for 14 d then, serum levels of some biochemical factors were evaluated. Pancreas samples were used for the determination of malondealdehyde (MDA) level, glutathione (GSH) content, superoxide dismutase, and catalase enzyme activities. Red blood cells (RBCs) and plasma were used for MDA estimation. Pancreatic α-amylase inhibition, DPPH (1,1-diphenyl-2-picrylhydrazyl), and FRAP (ferric reducing antioxidant power) assays were done. The total flavonoid content of the extract was determined by spectrophotometry.
Results: Extract (300 mg/kg) decreased serum glucose level (27.1%) significantly at 120 min in OGTT. Serum levels of creatinine, triglycerides, cholesterol, alanine amino transferase and MDA levels in plasma, RBCs, and pancreas significantly decreased in treated (300 mg/kg) diabetic rats, while pancreatic GSH content, superoxide dismutase, and catalase enzymatic activities increased (p < 0.05). The IC
50
values for the extract and butyl hydroxyanisole were 5.6 and 1.15 mg/mL in DPPH and 0.155 and 0.062 mg/mL in the FRAP methods, respectively. The extract had no inhibitory effect on α-amylase activity. The total amount of flavonoids of the extract was estimated to be 1.8% (g/g) on the basis of quercetin content.
Conclusion: Dracocephalum polychaetum shoot extract has antioxidant, antihyperlipidemic, and antilipid peroxidative properties.</abstract><cop>England</cop><pub>Informa Healthcare</pub><pmid>25901731</pmid><doi>10.3109/13880209.2015.1033561</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1388-0209 |
| ispartof | Pharmaceutical biology, 2016-02, Vol.54 (2), p.272-278 |
| issn | 1388-0209 1744-5116 |
| language | eng |
| recordid | cdi_crossref_primary_10_3109_13880209_2015_1033561 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Administration, Oral Alpha-amylase inhibitory activity Animals Antioxidants - administration & dosage Antioxidants - isolation & purification Antioxidants - therapeutic use Biomarkers - blood Biphenyl Compounds - chemistry Blood Glucose - analysis diabetes mellitus Diabetes Mellitus, Experimental - blood Diabetes Mellitus, Experimental - drug therapy Diabetes Mellitus, Experimental - metabolism Dose-Response Relationship, Drug Glucose Tolerance Test Hypoglycemic Agents - administration & dosage Hypoglycemic Agents - isolation & purification Hypoglycemic Agents - therapeutic use Hypolipidemic Agents - administration & dosage Hypolipidemic Agents - isolation & purification Hypolipidemic Agents - therapeutic use Lamiaceae - chemistry Lipid Peroxidation - drug effects Male Malondialdehyde - blood MDA medicinal plants pancreas Picrates - chemistry Plant Extracts - administration & dosage Plant Extracts - isolation & purification Plant Extracts - therapeutic use Plant Shoots - chemistry Rats, Wistar Streptozocin |
| title | Antidiabetic, antioxidant, and antilipid peroxidative activities of Dracocephalum polychaetum shoot extract in streptozotocin-induced diabetic rats: In vivo and in vitro studies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T05%3A24%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antidiabetic,%20antioxidant,%20and%20antilipid%20peroxidative%20activities%20of%20Dracocephalum%20polychaetum%20shoot%20extract%20in%20streptozotocin-induced%20diabetic%20rats:%20In%20vivo%20and%20in%20vitro%20studies&rft.jtitle=Pharmaceutical%20biology&rft.au=Pouraboli,%20Iran&rft.date=2016-02-01&rft.volume=54&rft.issue=2&rft.spage=272&rft.epage=278&rft.pages=272-278&rft.issn=1388-0209&rft.eissn=1744-5116&rft_id=info:doi/10.3109/13880209.2015.1033561&rft_dat=%3Cproquest_cross%3E1747328720%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1747328720&rft_id=info:pmid/25901731&rfr_iscdi=true |