Diospyros perigrena bark extract induced apoptosis in filarial parasite Setaria cervi through generation of reactive oxygen species
Abstract Context: Lymphatic filariasis is a major neglected tropical disease. Diospyros perigrena Gurke (Ebenaceae) was selected for antifilarial chemotherapy because of unavailability of proper medicine. In India, different parts of this plant were used for the treatment of diabetes, diarrhea, dyse...
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| Veröffentlicht in: | Pharmaceutical biology 2015-06, Vol.53 (6), p.813-823 |
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| creator | Saini, Prasanta Mukherjee, Niladri Mukherjee, Suprabhat Roy, Priya Gayen, Prajna Kumar, Deepak Pal, Bikas Chandra Sinha Babu, Santi P. |
| description | Abstract
Context: Lymphatic filariasis is a major neglected tropical disease. Diospyros perigrena Gurke (Ebenaceae) was selected for antifilarial chemotherapy because of unavailability of proper medicine. In India, different parts of this plant were used for the treatment of diabetes, diarrhea, dysentery, cholera, mouth ulcers, and wounds.
Objective: The present study was undertaken to access antifilarial potential and mechanism of action of n-butanol extract (NBE) of D. perigrena stem bark on Setaria cervi Rudolphi (Onchocercidae).
Materials and methods: In vitro efficacy and apoptotic mechanism were evaluated by Hoechst, TUNEL, DNA fragmentation assay, pro- and anti-apoptotic gene expression in NBE (250, 125, 62.5, 31.25, and 15.6 µg/ml)-treated S. cervi after 24 h of incubation. Reactive oxygen species (ROS) up-regulation was also determined by GSH, GST, SOD assays, and super oxide anion level.
Results: Significant in vitro antifilarial activity of NBE was found 50% inhibitory concentration (IC50): adult = 57.6 μg/ml, microfilariae (mf) = 56.1 μg/ml, and lethal dose (LD100) in mf is 187.17 μg/ml) after 24 h of treatment. NBF-induced apoptosis was proved by Hoechst, TUNEL, RT-PCR, and Western blot method. NBF (250 µg/ml) decreased the level of GSH (17.8%) and GST (65.4%), increased SOD activity (1.42-fold) and super oxide anion production (1.32-fold) in the treated parasite which culminated into ROS up-regulation.
Discussion and conclusion: NBE induced apoptosis in different life cycle stages of S. cervi. In future, a detailed study of NBF will give us a novel antifilarial compound which will be used for antifilarial chemotherapy. |
| doi_str_mv | 10.3109/13880209.2014.943244 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_3109_13880209_2014_943244</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1667962346</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-8dbd5f52fa044ceeba08f7926391746c7d3ac3c92127651f4f52cc26a81bd6943</originalsourceid><addsrcrecordid>eNp9kE1v1DAQhiMEoh_wDxDykUsWf8VJLiBUoCBV4gCcrYkz2XXJxmHstN0zfxxH2yJx6cnW-Jl3xk9RvBJ8owRv3wrVNFzydiO50JtWK6n1k-JU1FqXlRDmab5npFyZk-IsxmvOeaVU9bw4kVWdq7U6Lf589CHOBwqRzUh-SzgB64B-MbxLBC4xP_WLw57BHOYUoo-5wgY_AnkY2QwE0Sdk3zGtFeaQbjxLOwrLdse2OCFB8mFiYWCEOdDfIAt3h_zC4ozOY3xRPBtgjPjy_jwvfn7-9OPiS3n17fLrxYer0mnRpLLpu74aKjkA19ohdsCboW6lUW3-tHF1r8Ap10oha1OJQWfWOWmgEV1vsqDz4s0xd6bwe8GY7N5Hh-MIE4YlWmFM3RqptMmoPqIum4mEg53J74EOVnC76rcP-u2q3x7157bX9xOWbo_9v6YH3xl4fwT8NATaw22gsbcJDmOggWByPq7xj45491_CDmFMOweE9josNGWBj-_4F5Eaqps</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1667962346</pqid></control><display><type>article</type><title>Diospyros perigrena bark extract induced apoptosis in filarial parasite Setaria cervi through generation of reactive oxygen species</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Saini, Prasanta ; Mukherjee, Niladri ; Mukherjee, Suprabhat ; Roy, Priya ; Gayen, Prajna ; Kumar, Deepak ; Pal, Bikas Chandra ; Sinha Babu, Santi P.</creator><creatorcontrib>Saini, Prasanta ; Mukherjee, Niladri ; Mukherjee, Suprabhat ; Roy, Priya ; Gayen, Prajna ; Kumar, Deepak ; Pal, Bikas Chandra ; Sinha Babu, Santi P.</creatorcontrib><description>Abstract
Context: Lymphatic filariasis is a major neglected tropical disease. Diospyros perigrena Gurke (Ebenaceae) was selected for antifilarial chemotherapy because of unavailability of proper medicine. In India, different parts of this plant were used for the treatment of diabetes, diarrhea, dysentery, cholera, mouth ulcers, and wounds.
Objective: The present study was undertaken to access antifilarial potential and mechanism of action of n-butanol extract (NBE) of D. perigrena stem bark on Setaria cervi Rudolphi (Onchocercidae).
Materials and methods: In vitro efficacy and apoptotic mechanism were evaluated by Hoechst, TUNEL, DNA fragmentation assay, pro- and anti-apoptotic gene expression in NBE (250, 125, 62.5, 31.25, and 15.6 µg/ml)-treated S. cervi after 24 h of incubation. Reactive oxygen species (ROS) up-regulation was also determined by GSH, GST, SOD assays, and super oxide anion level.
Results: Significant in vitro antifilarial activity of NBE was found 50% inhibitory concentration (IC50): adult = 57.6 μg/ml, microfilariae (mf) = 56.1 μg/ml, and lethal dose (LD100) in mf is 187.17 μg/ml) after 24 h of treatment. NBF-induced apoptosis was proved by Hoechst, TUNEL, RT-PCR, and Western blot method. NBF (250 µg/ml) decreased the level of GSH (17.8%) and GST (65.4%), increased SOD activity (1.42-fold) and super oxide anion production (1.32-fold) in the treated parasite which culminated into ROS up-regulation.
Discussion and conclusion: NBE induced apoptosis in different life cycle stages of S. cervi. In future, a detailed study of NBF will give us a novel antifilarial compound which will be used for antifilarial chemotherapy.</description><identifier>ISSN: 1388-0209</identifier><identifier>EISSN: 1744-5116</identifier><identifier>DOI: 10.3109/13880209.2014.943244</identifier><identifier>PMID: 25720973</identifier><language>eng</language><publisher>England: Informa Healthcare USA, Inc</publisher><subject>1-Butanol ; Animals ; Antifilarial ; Apoptosis - drug effects ; Bisbenzimidazole ; cell death abnormal ; Coloring Agents ; Diospyros - chemistry ; DNA - drug effects ; DNA fragmentation ; Filariasis - drug therapy ; Filariasis - psychology ; Filaricides - pharmacology ; glutathione ; In Situ Nick-End Labeling ; Plant Bark - chemistry ; Plant Extracts - pharmacology ; Reactive Oxygen Species - metabolism ; relative movability ; Setaria Nematode - drug effects ; Setaria Nematode - metabolism ; Solvents ; superoxide dismutase ; Tetrazolium Salts ; Thiazoles</subject><ispartof>Pharmaceutical biology, 2015-06, Vol.53 (6), p.813-823</ispartof><rights>2015 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-8dbd5f52fa044ceeba08f7926391746c7d3ac3c92127651f4f52cc26a81bd6943</citedby><cites>FETCH-LOGICAL-c418t-8dbd5f52fa044ceeba08f7926391746c7d3ac3c92127651f4f52cc26a81bd6943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25720973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saini, Prasanta</creatorcontrib><creatorcontrib>Mukherjee, Niladri</creatorcontrib><creatorcontrib>Mukherjee, Suprabhat</creatorcontrib><creatorcontrib>Roy, Priya</creatorcontrib><creatorcontrib>Gayen, Prajna</creatorcontrib><creatorcontrib>Kumar, Deepak</creatorcontrib><creatorcontrib>Pal, Bikas Chandra</creatorcontrib><creatorcontrib>Sinha Babu, Santi P.</creatorcontrib><title>Diospyros perigrena bark extract induced apoptosis in filarial parasite Setaria cervi through generation of reactive oxygen species</title><title>Pharmaceutical biology</title><addtitle>Pharm Biol</addtitle><description>Abstract
Context: Lymphatic filariasis is a major neglected tropical disease. Diospyros perigrena Gurke (Ebenaceae) was selected for antifilarial chemotherapy because of unavailability of proper medicine. In India, different parts of this plant were used for the treatment of diabetes, diarrhea, dysentery, cholera, mouth ulcers, and wounds.
Objective: The present study was undertaken to access antifilarial potential and mechanism of action of n-butanol extract (NBE) of D. perigrena stem bark on Setaria cervi Rudolphi (Onchocercidae).
Materials and methods: In vitro efficacy and apoptotic mechanism were evaluated by Hoechst, TUNEL, DNA fragmentation assay, pro- and anti-apoptotic gene expression in NBE (250, 125, 62.5, 31.25, and 15.6 µg/ml)-treated S. cervi after 24 h of incubation. Reactive oxygen species (ROS) up-regulation was also determined by GSH, GST, SOD assays, and super oxide anion level.
Results: Significant in vitro antifilarial activity of NBE was found 50% inhibitory concentration (IC50): adult = 57.6 μg/ml, microfilariae (mf) = 56.1 μg/ml, and lethal dose (LD100) in mf is 187.17 μg/ml) after 24 h of treatment. NBF-induced apoptosis was proved by Hoechst, TUNEL, RT-PCR, and Western blot method. NBF (250 µg/ml) decreased the level of GSH (17.8%) and GST (65.4%), increased SOD activity (1.42-fold) and super oxide anion production (1.32-fold) in the treated parasite which culminated into ROS up-regulation.
Discussion and conclusion: NBE induced apoptosis in different life cycle stages of S. cervi. In future, a detailed study of NBF will give us a novel antifilarial compound which will be used for antifilarial chemotherapy.</description><subject>1-Butanol</subject><subject>Animals</subject><subject>Antifilarial</subject><subject>Apoptosis - drug effects</subject><subject>Bisbenzimidazole</subject><subject>cell death abnormal</subject><subject>Coloring Agents</subject><subject>Diospyros - chemistry</subject><subject>DNA - drug effects</subject><subject>DNA fragmentation</subject><subject>Filariasis - drug therapy</subject><subject>Filariasis - psychology</subject><subject>Filaricides - pharmacology</subject><subject>glutathione</subject><subject>In Situ Nick-End Labeling</subject><subject>Plant Bark - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>relative movability</subject><subject>Setaria Nematode - drug effects</subject><subject>Setaria Nematode - metabolism</subject><subject>Solvents</subject><subject>superoxide dismutase</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><issn>1388-0209</issn><issn>1744-5116</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhiMEoh_wDxDykUsWf8VJLiBUoCBV4gCcrYkz2XXJxmHstN0zfxxH2yJx6cnW-Jl3xk9RvBJ8owRv3wrVNFzydiO50JtWK6n1k-JU1FqXlRDmab5npFyZk-IsxmvOeaVU9bw4kVWdq7U6Lf589CHOBwqRzUh-SzgB64B-MbxLBC4xP_WLw57BHOYUoo-5wgY_AnkY2QwE0Sdk3zGtFeaQbjxLOwrLdse2OCFB8mFiYWCEOdDfIAt3h_zC4ozOY3xRPBtgjPjy_jwvfn7-9OPiS3n17fLrxYer0mnRpLLpu74aKjkA19ohdsCboW6lUW3-tHF1r8Ap10oha1OJQWfWOWmgEV1vsqDz4s0xd6bwe8GY7N5Hh-MIE4YlWmFM3RqptMmoPqIum4mEg53J74EOVnC76rcP-u2q3x7157bX9xOWbo_9v6YH3xl4fwT8NATaw22gsbcJDmOggWByPq7xj45491_CDmFMOweE9josNGWBj-_4F5Eaqps</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Saini, Prasanta</creator><creator>Mukherjee, Niladri</creator><creator>Mukherjee, Suprabhat</creator><creator>Roy, Priya</creator><creator>Gayen, Prajna</creator><creator>Kumar, Deepak</creator><creator>Pal, Bikas Chandra</creator><creator>Sinha Babu, Santi P.</creator><general>Informa Healthcare USA, Inc</general><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Diospyros perigrena bark extract induced apoptosis in filarial parasite Setaria cervi through generation of reactive oxygen species</title><author>Saini, Prasanta ; Mukherjee, Niladri ; Mukherjee, Suprabhat ; Roy, Priya ; Gayen, Prajna ; Kumar, Deepak ; Pal, Bikas Chandra ; Sinha Babu, Santi P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-8dbd5f52fa044ceeba08f7926391746c7d3ac3c92127651f4f52cc26a81bd6943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>1-Butanol</topic><topic>Animals</topic><topic>Antifilarial</topic><topic>Apoptosis - drug effects</topic><topic>Bisbenzimidazole</topic><topic>cell death abnormal</topic><topic>Coloring Agents</topic><topic>Diospyros - chemistry</topic><topic>DNA - drug effects</topic><topic>DNA fragmentation</topic><topic>Filariasis - drug therapy</topic><topic>Filariasis - psychology</topic><topic>Filaricides - pharmacology</topic><topic>glutathione</topic><topic>In Situ Nick-End Labeling</topic><topic>Plant Bark - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>relative movability</topic><topic>Setaria Nematode - drug effects</topic><topic>Setaria Nematode - metabolism</topic><topic>Solvents</topic><topic>superoxide dismutase</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saini, Prasanta</creatorcontrib><creatorcontrib>Mukherjee, Niladri</creatorcontrib><creatorcontrib>Mukherjee, Suprabhat</creatorcontrib><creatorcontrib>Roy, Priya</creatorcontrib><creatorcontrib>Gayen, Prajna</creatorcontrib><creatorcontrib>Kumar, Deepak</creatorcontrib><creatorcontrib>Pal, Bikas Chandra</creatorcontrib><creatorcontrib>Sinha Babu, Santi P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmaceutical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saini, Prasanta</au><au>Mukherjee, Niladri</au><au>Mukherjee, Suprabhat</au><au>Roy, Priya</au><au>Gayen, Prajna</au><au>Kumar, Deepak</au><au>Pal, Bikas Chandra</au><au>Sinha Babu, Santi P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diospyros perigrena bark extract induced apoptosis in filarial parasite Setaria cervi through generation of reactive oxygen species</atitle><jtitle>Pharmaceutical biology</jtitle><addtitle>Pharm Biol</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>53</volume><issue>6</issue><spage>813</spage><epage>823</epage><pages>813-823</pages><issn>1388-0209</issn><eissn>1744-5116</eissn><abstract>Abstract
Context: Lymphatic filariasis is a major neglected tropical disease. Diospyros perigrena Gurke (Ebenaceae) was selected for antifilarial chemotherapy because of unavailability of proper medicine. In India, different parts of this plant were used for the treatment of diabetes, diarrhea, dysentery, cholera, mouth ulcers, and wounds.
Objective: The present study was undertaken to access antifilarial potential and mechanism of action of n-butanol extract (NBE) of D. perigrena stem bark on Setaria cervi Rudolphi (Onchocercidae).
Materials and methods: In vitro efficacy and apoptotic mechanism were evaluated by Hoechst, TUNEL, DNA fragmentation assay, pro- and anti-apoptotic gene expression in NBE (250, 125, 62.5, 31.25, and 15.6 µg/ml)-treated S. cervi after 24 h of incubation. Reactive oxygen species (ROS) up-regulation was also determined by GSH, GST, SOD assays, and super oxide anion level.
Results: Significant in vitro antifilarial activity of NBE was found 50% inhibitory concentration (IC50): adult = 57.6 μg/ml, microfilariae (mf) = 56.1 μg/ml, and lethal dose (LD100) in mf is 187.17 μg/ml) after 24 h of treatment. NBF-induced apoptosis was proved by Hoechst, TUNEL, RT-PCR, and Western blot method. NBF (250 µg/ml) decreased the level of GSH (17.8%) and GST (65.4%), increased SOD activity (1.42-fold) and super oxide anion production (1.32-fold) in the treated parasite which culminated into ROS up-regulation.
Discussion and conclusion: NBE induced apoptosis in different life cycle stages of S. cervi. In future, a detailed study of NBF will give us a novel antifilarial compound which will be used for antifilarial chemotherapy.</abstract><cop>England</cop><pub>Informa Healthcare USA, Inc</pub><pmid>25720973</pmid><doi>10.3109/13880209.2014.943244</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 1-Butanol Animals Antifilarial Apoptosis - drug effects Bisbenzimidazole cell death abnormal Coloring Agents Diospyros - chemistry DNA - drug effects DNA fragmentation Filariasis - drug therapy Filariasis - psychology Filaricides - pharmacology glutathione In Situ Nick-End Labeling Plant Bark - chemistry Plant Extracts - pharmacology Reactive Oxygen Species - metabolism relative movability Setaria Nematode - drug effects Setaria Nematode - metabolism Solvents superoxide dismutase Tetrazolium Salts Thiazoles |
| title | Diospyros perigrena bark extract induced apoptosis in filarial parasite Setaria cervi through generation of reactive oxygen species |
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