Comparison of Process Parameters for Microencapsulation of Plasmid DNA in Poly(D,L-Lactic-co-Glycolic) Acid Microspheres
Abstract Poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres containing plasmid DNA encoding the firefly luciferase gene were prepared using the water-in-oil-in-water (w/o/w) double emulsion and solvent evaporation method. In this study, we investigated the effects of three process parameters on D...
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| Veröffentlicht in: | Journal of drug targeting 1999-01, Vol.7 (4), p.313-323 |
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| creator | Hsu, Yung-Yueh Hao, Tang Hedley, Mary Lynne |
| description | Abstract
Poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres containing plasmid DNA encoding the firefly luciferase gene were prepared using the water-in-oil-in-water (w/o/w) double emulsion and solvent evaporation method. In this study, we investigated the effects of three process parameters on DNA microencapsulation: (1) emulsification method used to generate the primary emulsion, (2) water/oil ratio during formation of the first emulsion, and (3) surfactant concentration used in the preparation of the second emulsion. The resulting formulations were also analyzed for microsphere size, encapsulation efficiency, and kinetics of DNA release. We found that although each process alteration resulted in encapsulation of biologically active, structurally intact DNA, the surfactant and water/oil ratio significantly affected the size, release kinetics and encapsulation efficiency of plasmid DNA. |
| doi_str_mv | 10.3109/10611869909085514 |
| format | Article |
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Poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres containing plasmid DNA encoding the firefly luciferase gene were prepared using the water-in-oil-in-water (w/o/w) double emulsion and solvent evaporation method. In this study, we investigated the effects of three process parameters on DNA microencapsulation: (1) emulsification method used to generate the primary emulsion, (2) water/oil ratio during formation of the first emulsion, and (3) surfactant concentration used in the preparation of the second emulsion. The resulting formulations were also analyzed for microsphere size, encapsulation efficiency, and kinetics of DNA release. We found that although each process alteration resulted in encapsulation of biologically active, structurally intact DNA, the surfactant and water/oil ratio significantly affected the size, release kinetics and encapsulation efficiency of plasmid DNA.</description><identifier>ISSN: 1061-186X</identifier><identifier>EISSN: 1029-2330</identifier><identifier>DOI: 10.3109/10611869909085514</identifier><identifier>PMID: 10682910</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Biocompatible Materials - chemistry ; DNA - immunology ; DNA - metabolism ; DNA delivery ; DNA immunization ; Double emulsion ; Drug Compounding - methods ; Drug Delivery Systems - methods ; Emulsions ; Lactic Acid - chemistry ; Luciferases - genetics ; Microencapsulation ; Microscopy, Electron, Scanning ; Microspheres ; Particle Size ; Plasmids - genetics ; Poly(D,L-lactic-co-glycolic) acid (PLGA) ; Polyglycolic Acid - chemistry ; polylactide-co-glycolide ; Polymers - chemistry ; Solubility ; Sonication ; Surface-Active Agents - pharmacology ; Time Factors</subject><ispartof>Journal of drug targeting, 1999-01, Vol.7 (4), p.313-323</ispartof><rights>1999 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-b064b001334c162a9192e9d235b1a3b65dce3f1410eef58ec48b19c8be2d5fbe3</citedby><cites>FETCH-LOGICAL-c433t-b064b001334c162a9192e9d235b1a3b65dce3f1410eef58ec48b19c8be2d5fbe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/10611869909085514$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/10611869909085514$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10682910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsu, Yung-Yueh</creatorcontrib><creatorcontrib>Hao, Tang</creatorcontrib><creatorcontrib>Hedley, Mary Lynne</creatorcontrib><title>Comparison of Process Parameters for Microencapsulation of Plasmid DNA in Poly(D,L-Lactic-co-Glycolic) Acid Microspheres</title><title>Journal of drug targeting</title><addtitle>J Drug Target</addtitle><description>Abstract
Poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres containing plasmid DNA encoding the firefly luciferase gene were prepared using the water-in-oil-in-water (w/o/w) double emulsion and solvent evaporation method. In this study, we investigated the effects of three process parameters on DNA microencapsulation: (1) emulsification method used to generate the primary emulsion, (2) water/oil ratio during formation of the first emulsion, and (3) surfactant concentration used in the preparation of the second emulsion. The resulting formulations were also analyzed for microsphere size, encapsulation efficiency, and kinetics of DNA release. We found that although each process alteration resulted in encapsulation of biologically active, structurally intact DNA, the surfactant and water/oil ratio significantly affected the size, release kinetics and encapsulation efficiency of plasmid DNA.</description><subject>Biocompatible Materials - chemistry</subject><subject>DNA - immunology</subject><subject>DNA - metabolism</subject><subject>DNA delivery</subject><subject>DNA immunization</subject><subject>Double emulsion</subject><subject>Drug Compounding - methods</subject><subject>Drug Delivery Systems - methods</subject><subject>Emulsions</subject><subject>Lactic Acid - chemistry</subject><subject>Luciferases - genetics</subject><subject>Microencapsulation</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microspheres</subject><subject>Particle Size</subject><subject>Plasmids - genetics</subject><subject>Poly(D,L-lactic-co-glycolic) acid (PLGA)</subject><subject>Polyglycolic Acid - chemistry</subject><subject>polylactide-co-glycolide</subject><subject>Polymers - chemistry</subject><subject>Solubility</subject><subject>Sonication</subject><subject>Surface-Active Agents - pharmacology</subject><subject>Time Factors</subject><issn>1061-186X</issn><issn>1029-2330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2r1DAUhoMo3g_9AW4kK_GC1ZwmzTToZpirV2HUWSi4K-npKZNL2tSkReff2zqzUERdJZDnfTg5L2OPQDyXIMwLEBqg1MYII8qiAHWHnYPITZZLKe4udw3ZDHw5Yxcp3QoBUoO4z87mhzI3IM7Z903oBhtdCj0PLd_FgJQS39loOxopJt6GyN87jIF6tEOavB3dCfY2da7h1x_W3PV8F_zh6fWzbba1ODrMMGQ3_oDBO7zia5zBn5o07ClSesDutdYneng6L9nnN68_bd5m24837zbrbYZKyjGrhVb1MrhUCDq3BkxOpsllUYOVtS4aJNmCAkHUFiWhKmswWNaUN0Vbk7xkT47eIYavE6Wx6lxC8t72FKZUaaPkaqXL_4KwUlpqlc8gHMHlMylSWw3RdTYeKhDV0kv1Ry9z5vFJPtUdNb8kjkXMwKsj4Pp54Z39FqJvqtEefIhttD26tLj_7n_5W3xP1o97tJGq2zDFft7wP6b7Aahtrcc</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Hsu, Yung-Yueh</creator><creator>Hao, Tang</creator><creator>Hedley, Mary Lynne</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19990101</creationdate><title>Comparison of Process Parameters for Microencapsulation of Plasmid DNA in Poly(D,L-Lactic-co-Glycolic) Acid Microspheres</title><author>Hsu, Yung-Yueh ; Hao, Tang ; Hedley, Mary Lynne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-b064b001334c162a9192e9d235b1a3b65dce3f1410eef58ec48b19c8be2d5fbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biocompatible Materials - chemistry</topic><topic>DNA - immunology</topic><topic>DNA - metabolism</topic><topic>DNA delivery</topic><topic>DNA immunization</topic><topic>Double emulsion</topic><topic>Drug Compounding - methods</topic><topic>Drug Delivery Systems - methods</topic><topic>Emulsions</topic><topic>Lactic Acid - chemistry</topic><topic>Luciferases - genetics</topic><topic>Microencapsulation</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microspheres</topic><topic>Particle Size</topic><topic>Plasmids - genetics</topic><topic>Poly(D,L-lactic-co-glycolic) acid (PLGA)</topic><topic>Polyglycolic Acid - chemistry</topic><topic>polylactide-co-glycolide</topic><topic>Polymers - chemistry</topic><topic>Solubility</topic><topic>Sonication</topic><topic>Surface-Active Agents - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsu, Yung-Yueh</creatorcontrib><creatorcontrib>Hao, Tang</creatorcontrib><creatorcontrib>Hedley, Mary Lynne</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of drug targeting</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsu, Yung-Yueh</au><au>Hao, Tang</au><au>Hedley, Mary Lynne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Process Parameters for Microencapsulation of Plasmid DNA in Poly(D,L-Lactic-co-Glycolic) Acid Microspheres</atitle><jtitle>Journal of drug targeting</jtitle><addtitle>J Drug Target</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>7</volume><issue>4</issue><spage>313</spage><epage>323</epage><pages>313-323</pages><issn>1061-186X</issn><eissn>1029-2330</eissn><abstract>Abstract
Poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres containing plasmid DNA encoding the firefly luciferase gene were prepared using the water-in-oil-in-water (w/o/w) double emulsion and solvent evaporation method. In this study, we investigated the effects of three process parameters on DNA microencapsulation: (1) emulsification method used to generate the primary emulsion, (2) water/oil ratio during formation of the first emulsion, and (3) surfactant concentration used in the preparation of the second emulsion. The resulting formulations were also analyzed for microsphere size, encapsulation efficiency, and kinetics of DNA release. We found that although each process alteration resulted in encapsulation of biologically active, structurally intact DNA, the surfactant and water/oil ratio significantly affected the size, release kinetics and encapsulation efficiency of plasmid DNA.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>10682910</pmid><doi>10.3109/10611869909085514</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of drug targeting, 1999-01, Vol.7 (4), p.313-323 |
| issn | 1061-186X 1029-2330 |
| language | eng |
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| source | Taylor & Francis; MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | Biocompatible Materials - chemistry DNA - immunology DNA - metabolism DNA delivery DNA immunization Double emulsion Drug Compounding - methods Drug Delivery Systems - methods Emulsions Lactic Acid - chemistry Luciferases - genetics Microencapsulation Microscopy, Electron, Scanning Microspheres Particle Size Plasmids - genetics Poly(D,L-lactic-co-glycolic) acid (PLGA) Polyglycolic Acid - chemistry polylactide-co-glycolide Polymers - chemistry Solubility Sonication Surface-Active Agents - pharmacology Time Factors |
| title | Comparison of Process Parameters for Microencapsulation of Plasmid DNA in Poly(D,L-Lactic-co-Glycolic) Acid Microspheres |
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