Stain Technology: A Combined Elastic, Fibrin and Collagen Stain

Stain Technology: A Combined Elastic, Fibrin and Collagen Stain

A method is described which demonstrates nuclei, elastic fibers, red blood cells, collagen and fibrin. Nuclei and elastic fibers are stained by a modified VerhoefPs elastic tissue stain which was previously developed and used in the elastic-Masson combination. Both early fibrin and red blood cells a...

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Veröffentlicht in:Biotechnic & histochemistry 1987, Vol.62 (6), p.365-368
Hauptverfasser: Garvey, Winsome, Fathi, Arleen, Bigelow, Francine, Carpenter, Blair, Jimenez, Carmencita
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container_end_page 368
container_issue 6
container_start_page 365
container_title Biotechnic & histochemistry
container_volume 62
creator Garvey, Winsome
Fathi, Arleen
Bigelow, Francine
Carpenter, Blair
Jimenez, Carmencita
description A method is described which demonstrates nuclei, elastic fibers, red blood cells, collagen and fibrin. Nuclei and elastic fibers are stained by a modified VerhoefPs elastic tissue stain which was previously developed and used in the elastic-Masson combination. Both early fibrin and red blood cells are shown by Hssamine fast yellow. Mature fibrin, some types of collagen and other cytoplasmic changes are stained by a combination of acid fuchsia, Biebrich scarlet and ponceau 2R, while old fibrin is demonstrated by the collagen stain. This method takes about 1 hr to perform and has the added advantage that several entities are clearly shown in a single slide.
doi_str_mv 10.3109/10520298709108026
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source Taylor & Francis:Master (3349 titles)
title Stain Technology: A Combined Elastic, Fibrin and Collagen Stain
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