Selective Staining of Hepatitis B Surface Antigen in Thick Epoxy Sections of Liver

Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjus...

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Veröffentlicht in:	Biotechnic & histochemistry 1977-01, Vol.52 (5), p.285-289
Hauptverfasser:	Shamoto, Mikihiro, Nishio, Haruko, Katoh, Yoshimitsu, Senba, Hideo, Ito, Madoka
Format:	Artikel
Sprache:	eng
Schlagworte:	Hepatitis B Surface Antigens - analysis Humans Liver - immunology Liver - ultrastructure Microscopy Microscopy, Electron Rosaniline Dyes Staining and Labeling
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container_title	Biotechnic & histochemistry
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creator	Shamoto, Mikihiro Nishio, Haruko Katoh, Yoshimitsu Senba, Hideo Ito, Madoka
description	Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.
doi_str_mv	10.3109/10520297709116795
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Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.</description><identifier>ISSN: 1052-0295</identifier><identifier>ISSN: 0038-9153</identifier><identifier>EISSN: 1473-7760</identifier><identifier>DOI: 10.3109/10520297709116795</identifier><identifier>PMID: 73237</identifier><language>eng</language><publisher>United States: Informa UK Ltd</publisher><subject>Hepatitis B Surface Antigens - analysis ; Humans ; Liver - immunology ; Liver - ultrastructure ; Microscopy ; Microscopy, Electron ; Rosaniline Dyes ; Staining and Labeling</subject><ispartof>Biotechnic & histochemistry, 1977-01, Vol.52 (5), p.285-289</ispartof><rights>1977 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c390t-8858325e961a5e57ded0109e4babce877de0c43baef18f5b761b80b6b1ee6f0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/10520297709116795$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/10520297709116795$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,60436,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/73237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shamoto, Mikihiro</creatorcontrib><creatorcontrib>Nishio, Haruko</creatorcontrib><creatorcontrib>Katoh, Yoshimitsu</creatorcontrib><creatorcontrib>Senba, Hideo</creatorcontrib><creatorcontrib>Ito, Madoka</creatorcontrib><title>Selective Staining of Hepatitis B Surface Antigen in Thick Epoxy Sections of Liver</title><title>Biotechnic & histochemistry</title><addtitle>Stain Technol</addtitle><description>Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. 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This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.</description><subject>Hepatitis B Surface Antigens - analysis</subject><subject>Humans</subject><subject>Liver - immunology</subject><subject>Liver - ultrastructure</subject><subject>Microscopy</subject><subject>Microscopy, Electron</subject><subject>Rosaniline Dyes</subject><subject>Staining and Labeling</subject><issn>1052-0295</issn><issn>0038-9153</issn><issn>1473-7760</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kNFKwzAUhoM4dE4fQLzJC1STZm1a9GaO6YSBYOd1SdOTLbNLR5Kpe3tTJ4KIXp3D-c_3c86P0Dkll4yS_IqSJCZxzjnJKU15nhygPh1yFnGeksPQBz0KC8kxOnFuRQjhWU6PUI-zmPE-eiqgAen1K-DCC220WeBW4SlshNdeO3yLi61VQgIeGa8XYLA2eL7U8gVPNu37Dhcd3hrXYbPgY09RT4nGwdlXHaDnu8l8PI1mj_cP49EskiwnPsqyJGNxAnlKRQIJr6Em4SMYVqKSkPEwIHLIKgGKZiqpeEqrjFRpRQFSRYANEN37Sts6Z0GVG6vXwu5KSsounPJXOIG52DObbbWG-pv4TCOoN3tVG9XatXhrbVOXXuya1iorjNSuM_7b_PoHvgTR-KUUFspVu7UmhPHPaR-GgoSE</recordid><startdate>19770101</startdate><enddate>19770101</enddate><creator>Shamoto, Mikihiro</creator><creator>Nishio, Haruko</creator><creator>Katoh, Yoshimitsu</creator><creator>Senba, Hideo</creator><creator>Ito, Madoka</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19770101</creationdate><title>Selective Staining of Hepatitis B Surface Antigen in Thick Epoxy Sections of Liver</title><author>Shamoto, Mikihiro ; Nishio, Haruko ; Katoh, Yoshimitsu ; Senba, Hideo ; Ito, Madoka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-8858325e961a5e57ded0109e4babce877de0c43baef18f5b761b80b6b1ee6f0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Hepatitis B Surface Antigens - analysis</topic><topic>Humans</topic><topic>Liver - immunology</topic><topic>Liver - ultrastructure</topic><topic>Microscopy</topic><topic>Microscopy, Electron</topic><topic>Rosaniline Dyes</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shamoto, Mikihiro</creatorcontrib><creatorcontrib>Nishio, Haruko</creatorcontrib><creatorcontrib>Katoh, Yoshimitsu</creatorcontrib><creatorcontrib>Senba, Hideo</creatorcontrib><creatorcontrib>Ito, Madoka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Biotechnic & histochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shamoto, Mikihiro</au><au>Nishio, Haruko</au><au>Katoh, Yoshimitsu</au><au>Senba, Hideo</au><au>Ito, Madoka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective Staining of Hepatitis B Surface Antigen in Thick Epoxy Sections of Liver</atitle><jtitle>Biotechnic & histochemistry</jtitle><addtitle>Stain Technol</addtitle><date>1977-01-01</date><risdate>1977</risdate><volume>52</volume><issue>5</issue><spage>285</spage><epage>289</epage><pages>285-289</pages><issn>1052-0295</issn><issn>0038-9153</issn><eissn>1473-7760</eissn><abstract>Hepatitis B surface antigen (HB2Ag) in epoxy-embedded liver tissues can be stained by aldehyde-fuchsin stain. Sections are oxidized in KMnO4 acidified with H2SO4, then bleached in NaHSO3, both at 70 C. Heating for oxidation and bleaching are absolutely necessary. Diluted aldehyde-fuchsin stain adjusted to pH 1.5 to 1.8 with NaOH is used for staining. HBsAg is specifically stained purple. Other components such as mitochondria and bile pigments are also stained, but are easily distinguished from HBsAg. This staining method is advantageous for the identification of HBsAg-positive cells for electron microscopic observation.</abstract><cop>United States</cop><pub>Informa UK Ltd</pub><pmid>73237</pmid><doi>10.3109/10520297709116795</doi><tpages>5</tpages></addata></record>
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source	MEDLINE; Taylor & Francis:Master (3349 titles)
subjects	Hepatitis B Surface Antigens - analysis Humans Liver - immunology Liver - ultrastructure Microscopy Microscopy, Electron Rosaniline Dyes Staining and Labeling
title	Selective Staining of Hepatitis B Surface Antigen in Thick Epoxy Sections of Liver
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