Tax Protein of Human T-Lymphotropic Virus Type I Triggers DNA Damage
Previous findings indicated that in vitro HTLV-I-infected cells are highly susceptible to spontaneous and chemically induced DNA-damage. To further study the role of different virus gene products in inducing chromosome abnormalities, MOLT-3 cells were transiently transfected with a tax expressing pl...
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| Veröffentlicht in: | Leukemia & lymphoma 1994, Vol.12 (3-4), p.281-286 |
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| container_title | Leukemia & lymphoma |
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| creator | Torino, Monica Leszl, Anna Chieco-Bianchi, Luigi Saggioro, Daniela Majone, Franca Turchetto, Lucia |
| description | Previous findings indicated that in vitro HTLV-I-infected cells are highly susceptible to spontaneous and chemically induced DNA-damage. To further study the role of different virus gene products in inducing chromosome abnormalities, MOLT-3 cells were transiently transfected with a tax expressing plasmid (pTax), and assayed for genetic damage by the micro-nucleus test. We found that pTax-transfected cells not only had a statistically higher baseline micronuclcus value than non-transfected control cells, but also were more susceptible to Mitomycin C (MMC)-induced DNA damage. Furthermore, the use of human serum containing anti-kinetochore antibodies disclosed that tax enhances the clastogenic effect of MMC. No increase in total micronuclcus frequency was observed when MMC treatment preceded pTax transfection, thus suggesting that the micronucleus increase might not be due to the additive effect of tax and MMC. These findings indicate that the viral tax protein could play an important role in inducing the chromosome damage frequently observed in HTLV-I-infected cells. |
| doi_str_mv | 10.3109/10428199409059600 |
| format | Article |
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To further study the role of different virus gene products in inducing chromosome abnormalities, MOLT-3 cells were transiently transfected with a tax expressing plasmid (pTax), and assayed for genetic damage by the micro-nucleus test. We found that pTax-transfected cells not only had a statistically higher baseline micronuclcus value than non-transfected control cells, but also were more susceptible to Mitomycin C (MMC)-induced DNA damage. Furthermore, the use of human serum containing anti-kinetochore antibodies disclosed that tax enhances the clastogenic effect of MMC. No increase in total micronuclcus frequency was observed when MMC treatment preceded pTax transfection, thus suggesting that the micronucleus increase might not be due to the additive effect of tax and MMC. These findings indicate that the viral tax protein could play an important role in inducing the chromosome damage frequently observed in HTLV-I-infected cells.</description><identifier>ISSN: 1042-8194</identifier><identifier>EISSN: 1029-2403</identifier><identifier>DOI: 10.3109/10428199409059600</identifier><identifier>PMID: 8167559</identifier><language>eng</language><publisher>United States: Informa UK Ltd</publisher><subject>Antibodies - pharmacology ; Cell Line ; Chloramphenicol O-Acetyltransferase - biosynthesis ; Chloramphenicol O-Acetyltransferase - metabolism ; DNA Damage ; Fluorescent Antibody Technique ; Gene Products, tax - biosynthesis ; Gene Products, tax - metabolism ; Genes, pX ; HTLV-I leukemogcncsis ; Human T-lymphotropic virus 1 - genetics ; Humans ; Lymphoma, T-Cell ; micronuclei ; Micronuclei, Chromosome-Defective - drug effects ; Micronuclei, Chromosome-Defective - physiology ; Micronuclei, Chromosome-Defective - ultrastructure ; Mitomycin - toxicity ; Plasmids ; tax protein ; Transfection ; Tumor Cells, Cultured</subject><ispartof>Leukemia & lymphoma, 1994, Vol.12 (3-4), p.281-286</ispartof><rights>1994 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-32a6ce8a368acf63aaa32beb8d012b5a0ff49da2360912ac7b58c9e70c4560c63</citedby><cites>FETCH-LOGICAL-c401t-32a6ce8a368acf63aaa32beb8d012b5a0ff49da2360912ac7b58c9e70c4560c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/10428199409059600$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/10428199409059600$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8167559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torino, Monica</creatorcontrib><creatorcontrib>Leszl, Anna</creatorcontrib><creatorcontrib>Chieco-Bianchi, Luigi</creatorcontrib><creatorcontrib>Saggioro, Daniela</creatorcontrib><creatorcontrib>Majone, Franca</creatorcontrib><creatorcontrib>Turchetto, Lucia</creatorcontrib><title>Tax Protein of Human T-Lymphotropic Virus Type I Triggers DNA Damage</title><title>Leukemia & lymphoma</title><addtitle>Leuk Lymphoma</addtitle><description>Previous findings indicated that in vitro HTLV-I-infected cells are highly susceptible to spontaneous and chemically induced DNA-damage. To further study the role of different virus gene products in inducing chromosome abnormalities, MOLT-3 cells were transiently transfected with a tax expressing plasmid (pTax), and assayed for genetic damage by the micro-nucleus test. We found that pTax-transfected cells not only had a statistically higher baseline micronuclcus value than non-transfected control cells, but also were more susceptible to Mitomycin C (MMC)-induced DNA damage. Furthermore, the use of human serum containing anti-kinetochore antibodies disclosed that tax enhances the clastogenic effect of MMC. No increase in total micronuclcus frequency was observed when MMC treatment preceded pTax transfection, thus suggesting that the micronucleus increase might not be due to the additive effect of tax and MMC. These findings indicate that the viral tax protein could play an important role in inducing the chromosome damage frequently observed in HTLV-I-infected cells.</description><subject>Antibodies - pharmacology</subject><subject>Cell Line</subject><subject>Chloramphenicol O-Acetyltransferase - biosynthesis</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>DNA Damage</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Products, tax - biosynthesis</subject><subject>Gene Products, tax - metabolism</subject><subject>Genes, pX</subject><subject>HTLV-I leukemogcncsis</subject><subject>Human T-lymphotropic virus 1 - genetics</subject><subject>Humans</subject><subject>Lymphoma, T-Cell</subject><subject>micronuclei</subject><subject>Micronuclei, Chromosome-Defective - drug effects</subject><subject>Micronuclei, Chromosome-Defective - physiology</subject><subject>Micronuclei, Chromosome-Defective - ultrastructure</subject><subject>Mitomycin - toxicity</subject><subject>Plasmids</subject><subject>tax protein</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>1042-8194</issn><issn>1029-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKw0AQhhdRaq0-gAdhXyA6u9lss-iltGqFoh6i1zDZbNqUJhs2CZq3N6FFENHTDMz__QwfIZcMrn0G6oaB4CFTSoCCQEmAIzJmwJXHBfjHwy641wfEKTmr6y3AkOIjMgqZnAaBGpNFhJ_01dnG5CW1GV22BZY08lZdUW1s42yVa_qeu7amUVcZ-kQjl6_XxtV08TyjCyxwbc7JSYa72lwc5oS8PdxH86W3enl8ms9WnhbAGs_nKLUJ0Zch6kz6iOjzxCRhCownAUKWCZUi9yUoxlFPkyDUykxBi0CClv6EsH2vdrauncniyuUFui5mEA9C4l9CeuZqz1RtUpj0mzgY6O93-3teZtYV-GHdLo0b7HbWZQ5LnddD9d_1tz_wjcFds9HoTLy1rSt7Hf889wWycn9B</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Torino, Monica</creator><creator>Leszl, Anna</creator><creator>Chieco-Bianchi, Luigi</creator><creator>Saggioro, Daniela</creator><creator>Majone, Franca</creator><creator>Turchetto, Lucia</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1994</creationdate><title>Tax Protein of Human T-Lymphotropic Virus Type I Triggers DNA Damage</title><author>Torino, Monica ; Leszl, Anna ; Chieco-Bianchi, Luigi ; Saggioro, Daniela ; Majone, Franca ; Turchetto, Lucia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-32a6ce8a368acf63aaa32beb8d012b5a0ff49da2360912ac7b58c9e70c4560c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibodies - pharmacology</topic><topic>Cell Line</topic><topic>Chloramphenicol O-Acetyltransferase - biosynthesis</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>DNA Damage</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Products, tax - biosynthesis</topic><topic>Gene Products, tax - metabolism</topic><topic>Genes, pX</topic><topic>HTLV-I leukemogcncsis</topic><topic>Human T-lymphotropic virus 1 - genetics</topic><topic>Humans</topic><topic>Lymphoma, T-Cell</topic><topic>micronuclei</topic><topic>Micronuclei, Chromosome-Defective - drug effects</topic><topic>Micronuclei, Chromosome-Defective - physiology</topic><topic>Micronuclei, Chromosome-Defective - ultrastructure</topic><topic>Mitomycin - toxicity</topic><topic>Plasmids</topic><topic>tax protein</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torino, Monica</creatorcontrib><creatorcontrib>Leszl, Anna</creatorcontrib><creatorcontrib>Chieco-Bianchi, Luigi</creatorcontrib><creatorcontrib>Saggioro, Daniela</creatorcontrib><creatorcontrib>Majone, Franca</creatorcontrib><creatorcontrib>Turchetto, Lucia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Leukemia & lymphoma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torino, Monica</au><au>Leszl, Anna</au><au>Chieco-Bianchi, Luigi</au><au>Saggioro, Daniela</au><au>Majone, Franca</au><au>Turchetto, Lucia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tax Protein of Human T-Lymphotropic Virus Type I Triggers DNA Damage</atitle><jtitle>Leukemia & lymphoma</jtitle><addtitle>Leuk Lymphoma</addtitle><date>1994</date><risdate>1994</risdate><volume>12</volume><issue>3-4</issue><spage>281</spage><epage>286</epage><pages>281-286</pages><issn>1042-8194</issn><eissn>1029-2403</eissn><abstract>Previous findings indicated that in vitro HTLV-I-infected cells are highly susceptible to spontaneous and chemically induced DNA-damage. To further study the role of different virus gene products in inducing chromosome abnormalities, MOLT-3 cells were transiently transfected with a tax expressing plasmid (pTax), and assayed for genetic damage by the micro-nucleus test. We found that pTax-transfected cells not only had a statistically higher baseline micronuclcus value than non-transfected control cells, but also were more susceptible to Mitomycin C (MMC)-induced DNA damage. Furthermore, the use of human serum containing anti-kinetochore antibodies disclosed that tax enhances the clastogenic effect of MMC. No increase in total micronuclcus frequency was observed when MMC treatment preceded pTax transfection, thus suggesting that the micronucleus increase might not be due to the additive effect of tax and MMC. These findings indicate that the viral tax protein could play an important role in inducing the chromosome damage frequently observed in HTLV-I-infected cells.</abstract><cop>United States</cop><pub>Informa UK Ltd</pub><pmid>8167559</pmid><doi>10.3109/10428199409059600</doi><tpages>6</tpages></addata></record> |
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| ispartof | Leukemia & lymphoma, 1994, Vol.12 (3-4), p.281-286 |
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| language | eng |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Access via Taylor & Francis |
| subjects | Antibodies - pharmacology Cell Line Chloramphenicol O-Acetyltransferase - biosynthesis Chloramphenicol O-Acetyltransferase - metabolism DNA Damage Fluorescent Antibody Technique Gene Products, tax - biosynthesis Gene Products, tax - metabolism Genes, pX HTLV-I leukemogcncsis Human T-lymphotropic virus 1 - genetics Humans Lymphoma, T-Cell micronuclei Micronuclei, Chromosome-Defective - drug effects Micronuclei, Chromosome-Defective - physiology Micronuclei, Chromosome-Defective - ultrastructure Mitomycin - toxicity Plasmids tax protein Transfection Tumor Cells, Cultured |
| title | Tax Protein of Human T-Lymphotropic Virus Type I Triggers DNA Damage |
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