Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus
We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic...
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| Veröffentlicht in: | Stress (Amsterdam, Netherlands) Netherlands), 2010-07, Vol.13 (4), p.281-292 |
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| creator | Todoroki, Miwako Ueta, Yoichi Fujihara, Hiroaki Otsubo, Hiroki Shibata, Minori Hashimoto, Hirofumi Kobayashi, Mizuki Sakamoto, Hirotaka Kawata, Mitsuhiro Dayanithi, Govindan Murphy, David Hiro, Hisanori Takahashi, Ken Nagata, Shoji |
| description | We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 12-48 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress. |
| doi_str_mv | 10.3109/10253890903383406 |
| format | Article |
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In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 12-48 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress.</description><identifier>ISSN: 1025-3890</identifier><identifier>EISSN: 1607-8888</identifier><identifier>DOI: 10.3109/10253890903383406</identifier><identifier>PMID: 20536330</identifier><language>eng</language><publisher>England: Informa</publisher><subject>Animals ; Animals, Genetically Modified ; Arginine Vasopressin - genetics ; Colchicine ; Colchicine - administration & dosage ; Colchicine - pharmacology ; Female ; green fluorescent protein ; Green Fluorescent Proteins - genetics ; hypothalamus ; Injections, Intraventricular ; locus coeruleus ; Locus Coeruleus - metabolism ; noradrenaline ; Osmolar Concentration ; Paraventricular Hypothalamic Nucleus - metabolism ; Rats ; Rats, Wistar ; Recombinant Fusion Proteins - genetics ; Restraint, Physical ; RNA, Messenger - metabolism ; Sleep Deprivation ; stress ; Stress, Psychological ; Supraoptic Nucleus - metabolism ; vasopressin</subject><ispartof>Stress (Amsterdam, Netherlands), 2010-07, Vol.13 (4), p.281-292</ispartof><rights>Informa Healthcare USA, Inc. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-4b7857daf0d720121bdc55f23ec942931d8ae70670880fa7a1435516486a9cea3</citedby><cites>FETCH-LOGICAL-c514t-4b7857daf0d720121bdc55f23ec942931d8ae70670880fa7a1435516486a9cea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20536330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Todoroki, Miwako</creatorcontrib><creatorcontrib>Ueta, Yoichi</creatorcontrib><creatorcontrib>Fujihara, Hiroaki</creatorcontrib><creatorcontrib>Otsubo, Hiroki</creatorcontrib><creatorcontrib>Shibata, Minori</creatorcontrib><creatorcontrib>Hashimoto, Hirofumi</creatorcontrib><creatorcontrib>Kobayashi, Mizuki</creatorcontrib><creatorcontrib>Sakamoto, Hirotaka</creatorcontrib><creatorcontrib>Kawata, Mitsuhiro</creatorcontrib><creatorcontrib>Dayanithi, Govindan</creatorcontrib><creatorcontrib>Murphy, David</creatorcontrib><creatorcontrib>Hiro, Hisanori</creatorcontrib><creatorcontrib>Takahashi, Ken</creatorcontrib><creatorcontrib>Nagata, Shoji</creatorcontrib><title>Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus</title><title>Stress (Amsterdam, Netherlands)</title><addtitle>Stress</addtitle><description>We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 12-48 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Arginine Vasopressin - genetics</subject><subject>Colchicine</subject><subject>Colchicine - administration & dosage</subject><subject>Colchicine - pharmacology</subject><subject>Female</subject><subject>green fluorescent protein</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>hypothalamus</subject><subject>Injections, Intraventricular</subject><subject>locus coeruleus</subject><subject>Locus Coeruleus - metabolism</subject><subject>noradrenaline</subject><subject>Osmolar Concentration</subject><subject>Paraventricular Hypothalamic Nucleus - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Restraint, Physical</subject><subject>RNA, Messenger - metabolism</subject><subject>Sleep Deprivation</subject><subject>stress</subject><subject>Stress, Psychological</subject><subject>Supraoptic Nucleus - metabolism</subject><subject>vasopressin</subject><issn>1025-3890</issn><issn>1607-8888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1v1DAQxS0Eoh_wB3BBvnEKjOM4cQQXVLW0UqVe4BzNOuNdV1l78Qeo_z2OtiAhRH0Za-a9n54eY28EvJcCxg8CWiX1CCNIqWUH_TN2KnoYGl3f8_qv92YVnLCzlO4BoFfQvWQnLSjZSwmnLN_4uZjsgufB8rwjjnHrvPPEf2AKh0gpOd-Q36E3NPNtJPLcLiXUiyGf-SGGTK7uSlopOaJPW6r-ult5ETNfgimJm0CxLFTSK_bC4pLo9eM8Z9-uLr9eXDe3d19uLj7fNkaJLjfdZtBqmNHCPLQgWrGZjVK2lWTGrh2lmDXSAP0AWoPFAUUnlRJ9p3scDaE8Z--O3Jrxe6GUp72roZcFPYWSpkHKVnejGqtSHJUmhpQi2ekQ3R7jwyRgWrue_um6et4-0stmT_Mfx-9yq-DTUeC8DXGPP0Nc5injwxKirTUZl1b2__kf_7LvCJe8Mxhpug8l-trcE-l-AVgvoKA</recordid><startdate>20100701</startdate><enddate>20100701</enddate><creator>Todoroki, Miwako</creator><creator>Ueta, Yoichi</creator><creator>Fujihara, Hiroaki</creator><creator>Otsubo, Hiroki</creator><creator>Shibata, Minori</creator><creator>Hashimoto, Hirofumi</creator><creator>Kobayashi, Mizuki</creator><creator>Sakamoto, Hirotaka</creator><creator>Kawata, Mitsuhiro</creator><creator>Dayanithi, Govindan</creator><creator>Murphy, David</creator><creator>Hiro, Hisanori</creator><creator>Takahashi, Ken</creator><creator>Nagata, Shoji</creator><general>Informa</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100701</creationdate><title>Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus</title><author>Todoroki, Miwako ; Ueta, Yoichi ; Fujihara, Hiroaki ; Otsubo, Hiroki ; Shibata, Minori ; Hashimoto, Hirofumi ; Kobayashi, Mizuki ; Sakamoto, Hirotaka ; Kawata, Mitsuhiro ; Dayanithi, Govindan ; Murphy, David ; Hiro, Hisanori ; Takahashi, Ken ; Nagata, Shoji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-4b7857daf0d720121bdc55f23ec942931d8ae70670880fa7a1435516486a9cea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Arginine Vasopressin - genetics</topic><topic>Colchicine</topic><topic>Colchicine - administration & dosage</topic><topic>Colchicine - pharmacology</topic><topic>Female</topic><topic>green fluorescent protein</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>hypothalamus</topic><topic>Injections, Intraventricular</topic><topic>locus coeruleus</topic><topic>Locus Coeruleus - metabolism</topic><topic>noradrenaline</topic><topic>Osmolar Concentration</topic><topic>Paraventricular Hypothalamic Nucleus - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Restraint, Physical</topic><topic>RNA, Messenger - metabolism</topic><topic>Sleep Deprivation</topic><topic>stress</topic><topic>Stress, Psychological</topic><topic>Supraoptic Nucleus - metabolism</topic><topic>vasopressin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Todoroki, Miwako</creatorcontrib><creatorcontrib>Ueta, Yoichi</creatorcontrib><creatorcontrib>Fujihara, Hiroaki</creatorcontrib><creatorcontrib>Otsubo, Hiroki</creatorcontrib><creatorcontrib>Shibata, Minori</creatorcontrib><creatorcontrib>Hashimoto, Hirofumi</creatorcontrib><creatorcontrib>Kobayashi, Mizuki</creatorcontrib><creatorcontrib>Sakamoto, Hirotaka</creatorcontrib><creatorcontrib>Kawata, Mitsuhiro</creatorcontrib><creatorcontrib>Dayanithi, Govindan</creatorcontrib><creatorcontrib>Murphy, David</creatorcontrib><creatorcontrib>Hiro, Hisanori</creatorcontrib><creatorcontrib>Takahashi, Ken</creatorcontrib><creatorcontrib>Nagata, Shoji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Stress (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Todoroki, Miwako</au><au>Ueta, Yoichi</au><au>Fujihara, Hiroaki</au><au>Otsubo, Hiroki</au><au>Shibata, Minori</au><au>Hashimoto, Hirofumi</au><au>Kobayashi, Mizuki</au><au>Sakamoto, Hirotaka</au><au>Kawata, Mitsuhiro</au><au>Dayanithi, Govindan</au><au>Murphy, David</au><au>Hiro, Hisanori</au><au>Takahashi, Ken</au><au>Nagata, Shoji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus</atitle><jtitle>Stress (Amsterdam, Netherlands)</jtitle><addtitle>Stress</addtitle><date>2010-07-01</date><risdate>2010</risdate><volume>13</volume><issue>4</issue><spage>281</spage><epage>292</epage><pages>281-292</pages><issn>1025-3890</issn><eissn>1607-8888</eissn><abstract>We examined the effects of intracerebroventricular (i.c.v.) administration of colchicine on the expression of the arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene in rats. In rats administered i.c.v. vehicle (control), eGFP fluorescence was observed in the supraoptic nucleus (SON), the magnocellular division of the paraventricular nucleus (PVN), the suprachiasmatic nucleus (SCN), the median eminence (ME) and the posterior pituitary. Two days after i.c.v. administration of colchicine, eGFP fluorescence was markedly increased in the SON, the magnocellular and parvocellular divisions of the PVN, the SCN, the ME and the locus coeruleus (LC). Immunohistochemical staining for eGFP confirmed the distribution of fluorescence in both groups. In the colchicines-administered groups, immunohistochemistry for tyrosine hydroxylase (TH) revealed that the eGFP fluorescence was co-localised with TH-immunoreactivity in the LC. Similarly, in situ hybridization histochemistry for eGFP mRNA revealed a significant increase in gene expression in the LC, the SON and the PVN 12-48 h after administration of colchicine. Our results indicate that the synthesis of AVP-eGFP is upregulated in noradrenergic neurones in the LC after colchicine administration. This implies that AVP and noradrenaline, originating from LC neurones, might play a role in response to chronic stress.</abstract><cop>England</cop><pub>Informa</pub><pmid>20536330</pmid><doi>10.3109/10253890903383406</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Genetically Modified Arginine Vasopressin - genetics Colchicine Colchicine - administration & dosage Colchicine - pharmacology Female green fluorescent protein Green Fluorescent Proteins - genetics hypothalamus Injections, Intraventricular locus coeruleus Locus Coeruleus - metabolism noradrenaline Osmolar Concentration Paraventricular Hypothalamic Nucleus - metabolism Rats Rats, Wistar Recombinant Fusion Proteins - genetics Restraint, Physical RNA, Messenger - metabolism Sleep Deprivation stress Stress, Psychological Supraoptic Nucleus - metabolism vasopressin |
| title | Induction of the arginine vasopressin-enhanced green fluorescent protein fusion transgene in the rat locus coeruleus |
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