Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays

Polymerase chain reaction (PCR) assays were used to amplify herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) sequences in DNA extracted from formalin-fixed, paraffin embedded corneas of patients undergoing corneal transplantation. PCR reamplification with an internal (nested) set of primers...

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Veröffentlicht in:	Current eye research 1991, Vol.10 (s1), p.15-21
Hauptverfasser:	Cantin, Edouard M., Chen, Jian, McNeill, James, Willey, Dru E., Openshaw, Harry
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Aged, 80 and over Base Sequence Biological and medical sciences Cornea - microbiology Corneal Transplantation DNA, Viral - analysis Female Graft Rejection Human viral diseases Humans Infectious diseases Keratitis, Dendritic - microbiology Male Medical sciences Middle Aged Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction Simplexvirus - enzymology Simplexvirus - genetics Simplexvirus - isolation & purification Thymidine Kinase - genetics Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
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