Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays
Polymerase chain reaction (PCR) assays were used to amplify herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) sequences in DNA extracted from formalin-fixed, paraffin embedded corneas of patients undergoing corneal transplantation. PCR reamplification with an internal (nested) set of primers...
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| Veröffentlicht in: | Current eye research 1991, Vol.10 (s1), p.15-21 |
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| creator | Cantin, Edouard M. Chen, Jian McNeill, James Willey, Dru E. Openshaw, Harry |
| description | Polymerase chain reaction (PCR) assays were used to amplify herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) sequences in DNA extracted from formalin-fixed, paraffin embedded corneas of patients undergoing corneal transplantation. PCR reamplification with an internal (nested) set of primers was required for detection in 10 of the 12 positive corneas indicating very low abundance of viral sequences. Three of the positive corneal samples were from failed corneal grafts. Overall, TK sequences were detected in 8 of 11 corneas from subjects with a past history of herpes keratitis and in 4 of 11 corneas from subjects with no past history of herpetic eye disease. |
| doi_str_mv | 10.3109/02713689109020353 |
| format | Article |
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PCR reamplification with an internal (nested) set of primers was required for detection in 10 of the 12 positive corneas indicating very low abundance of viral sequences. Three of the positive corneal samples were from failed corneal grafts. Overall, TK sequences were detected in 8 of 11 corneas from subjects with a past history of herpes keratitis and in 4 of 11 corneas from subjects with no past history of herpetic eye disease.</description><identifier>ISSN: 0271-3683</identifier><identifier>EISSN: 1460-2202</identifier><identifier>DOI: 10.3109/02713689109020353</identifier><identifier>PMID: 1650662</identifier><identifier>CODEN: CEYRDM</identifier><language>eng</language><publisher>Lisse: Informa UK Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Base Sequence ; Biological and medical sciences ; Cornea - microbiology ; Corneal Transplantation ; DNA, Viral - analysis ; Female ; Graft Rejection ; Human viral diseases ; Humans ; Infectious diseases ; Keratitis, Dendritic - microbiology ; Male ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; Oligonucleotide Probes ; Polymerase Chain Reaction ; Simplexvirus - enzymology ; Simplexvirus - genetics ; Simplexvirus - isolation & purification ; Thymidine Kinase - genetics ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><ispartof>Current eye research, 1991, Vol.10 (s1), p.15-21</ispartof><rights>1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-b3ae0472393255c78f70efd2ac85c1cd3a53f3f7de021a5f4a317b4777cd7563</citedby><cites>FETCH-LOGICAL-c431t-b3ae0472393255c78f70efd2ac85c1cd3a53f3f7de021a5f4a317b4777cd7563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/02713689109020353$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/02713689109020353$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>310,311,315,781,785,790,791,4025,4051,4052,23935,23936,25145,27928,27929,27930,59652,59758,60441,60547,61226,61261,61407,61442</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19724848$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1650662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cantin, Edouard M.</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>McNeill, James</creatorcontrib><creatorcontrib>Willey, Dru E.</creatorcontrib><creatorcontrib>Openshaw, Harry</creatorcontrib><title>Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays</title><title>Current eye research</title><addtitle>Curr Eye Res</addtitle><description>Polymerase chain reaction (PCR) assays were used to amplify herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) sequences in DNA extracted from formalin-fixed, paraffin embedded corneas of patients undergoing corneal transplantation. PCR reamplification with an internal (nested) set of primers was required for detection in 10 of the 12 positive corneas indicating very low abundance of viral sequences. Three of the positive corneal samples were from failed corneal grafts. Overall, TK sequences were detected in 8 of 11 corneas from subjects with a past history of herpes keratitis and in 4 of 11 corneas from subjects with no past history of herpetic eye disease.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cornea - microbiology</subject><subject>Corneal Transplantation</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Graft Rejection</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Keratitis, Dendritic - microbiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotide Probes</subject><subject>Polymerase Chain Reaction</subject><subject>Simplexvirus - enzymology</subject><subject>Simplexvirus - genetics</subject><subject>Simplexvirus - isolation & purification</subject><subject>Thymidine Kinase - genetics</subject><subject>Viral diseases</subject><subject>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><issn>0271-3683</issn><issn>1460-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS0EKtPCA7BA8gZ2Af8kcUawqVpKK1XtpvvojnOtcZXEwdcB8vZ4lEEVQurKls93ro-PGXsnxSctxfazUEbqutnmvVBCV_oF28iyFoVSQr1km4NeZEC_ZqdEj0IcDsoTdiLrStS12rB0iQlt8mHkwfE9xgmJkx-mHn_znz7OxC_vzjnhjxlHmzU_chviiNDzFGGkqYcx8YjWTx7HRHy38Cn0y4ARCLndQ3ZEhPUOIIKF3rBXDnrCt8f1jD1cfXu4uC5u77_fXJzfFrbUMhU7DShKo_RWq6qypnFGoOsU2Kay0nYaKu20Mx0KJaFyJWhpdqUxxnamqvUZ-7iOnWLI8Sm1gyeLfU6MYaa2EUY2SqkMyhW0MRBFdO0U_QBxaaVoD0W3_xWdPe-Pw-fdgN2TY2026x-OOpCF3uWurKcnbGtU2ZRN5r6unB9diAP8CrHv2gRLH-Jfk34uxpd_7Pv8MWlvIWL7GOY45nqfecQfKk-sfA</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Cantin, Edouard M.</creator><creator>Chen, Jian</creator><creator>McNeill, James</creator><creator>Willey, Dru E.</creator><creator>Openshaw, Harry</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Swets & Zeitlinger</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays</title><author>Cantin, Edouard M. ; Chen, Jian ; McNeill, James ; Willey, Dru E. ; Openshaw, Harry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-b3ae0472393255c78f70efd2ac85c1cd3a53f3f7de021a5f4a317b4777cd7563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cornea - microbiology</topic><topic>Corneal Transplantation</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>Graft Rejection</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Keratitis, Dendritic - microbiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Probes</topic><topic>Polymerase Chain Reaction</topic><topic>Simplexvirus - enzymology</topic><topic>Simplexvirus - genetics</topic><topic>Simplexvirus - isolation & purification</topic><topic>Thymidine Kinase - genetics</topic><topic>Viral diseases</topic><topic>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cantin, Edouard M.</creatorcontrib><creatorcontrib>Chen, Jian</creatorcontrib><creatorcontrib>McNeill, James</creatorcontrib><creatorcontrib>Willey, Dru E.</creatorcontrib><creatorcontrib>Openshaw, Harry</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cantin, Edouard M.</au><au>Chen, Jian</au><au>McNeill, James</au><au>Willey, Dru E.</au><au>Openshaw, Harry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>1991</date><risdate>1991</risdate><volume>10</volume><issue>s1</issue><spage>15</spage><epage>21</epage><pages>15-21</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><coden>CEYRDM</coden><abstract>Polymerase chain reaction (PCR) assays were used to amplify herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) sequences in DNA extracted from formalin-fixed, paraffin embedded corneas of patients undergoing corneal transplantation. PCR reamplification with an internal (nested) set of primers was required for detection in 10 of the 12 positive corneas indicating very low abundance of viral sequences. Three of the positive corneal samples were from failed corneal grafts. Overall, TK sequences were detected in 8 of 11 corneas from subjects with a past history of herpes keratitis and in 4 of 11 corneas from subjects with no past history of herpetic eye disease.</abstract><cop>Lisse</cop><pub>Informa UK Ltd</pub><pmid>1650662</pmid><doi>10.3109/02713689109020353</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0271-3683 |
| ispartof | Current eye research, 1991, Vol.10 (s1), p.15-21 |
| issn | 0271-3683 1460-2202 |
| language | eng |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Access via Taylor & Francis |
| subjects | Adult Aged Aged, 80 and over Base Sequence Biological and medical sciences Cornea - microbiology Corneal Transplantation DNA, Viral - analysis Female Graft Rejection Human viral diseases Humans Infectious diseases Keratitis, Dendritic - microbiology Male Medical sciences Middle Aged Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction Simplexvirus - enzymology Simplexvirus - genetics Simplexvirus - isolation & purification Thymidine Kinase - genetics Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye |
| title | Detection of herpes simplex virus DNA sequences in corneal transplant recipients by polymerase chain reaction assays |
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