Secretions from Primary Hamster Tracheal Surface Epithelial Cells in Culture: Mucin-Like Glycoproteins, Proteoglycans, and Lipids
Surface epithelial cells dissociated from hamster tracheas and grown on a thick collagen gel in the presence of 5% fetal bovine serum become highly enriched with secretory cells at confluence. In the present communication, we have analyzed secretory products from this primary hamster tracheal surfac...
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| Veröffentlicht in: | Experimental lung research 1989-01, Vol.15 (2), p.299-314 |
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| creator | Kim, Kwang C. Opaskar-Hincman, Helen Bhaskar, K. Ramakrishnan |
| description | Surface epithelial cells dissociated from hamster tracheas and grown on a thick collagen gel in the presence of 5% fetal bovine serum become highly enriched with secretory cells at confluence. In the present communication, we have analyzed secretory products from this primary hamster tracheal surface epithelial (HTSE) cell culture. The secreted glycoconjugates included high-molecular-weight mucin-like glycoproteins (HMW MLGP) and proteoglycans that comprised 22% and 5% of the total fHJglycoconjugates secreted when [3H]glucosamine was added as a metabolic precursor. Among the proteoglycans were hyaluronic acids (53%), heparan sulfate proteoglycans (29%), and chron-droitin sulfate proteoglycans (18%). Chondroitin sulfates were mostly 4-sulfated. On the other hand, the secreted lipids included cholesterol, phospholipids, and glycolipids, and most of them were associated with HMW MLGP. |
| doi_str_mv | 10.3109/01902148909087860 |
| format | Article |
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Ramakrishnan</creator><creatorcontrib>Kim, Kwang C. ; Opaskar-Hincman, Helen ; Bhaskar, K. Ramakrishnan</creatorcontrib><description>Surface epithelial cells dissociated from hamster tracheas and grown on a thick collagen gel in the presence of 5% fetal bovine serum become highly enriched with secretory cells at confluence. In the present communication, we have analyzed secretory products from this primary hamster tracheal surface epithelial (HTSE) cell culture. The secreted glycoconjugates included high-molecular-weight mucin-like glycoproteins (HMW MLGP) and proteoglycans that comprised 22% and 5% of the total fHJglycoconjugates secreted when [3H]glucosamine was added as a metabolic precursor. Among the proteoglycans were hyaluronic acids (53%), heparan sulfate proteoglycans (29%), and chron-droitin sulfate proteoglycans (18%). Chondroitin sulfates were mostly 4-sulfated. On the other hand, the secreted lipids included cholesterol, phospholipids, and glycolipids, and most of them were associated with HMW MLGP.</description><identifier>ISSN: 0190-2148</identifier><identifier>EISSN: 1521-0499</identifier><identifier>DOI: 10.3109/01902148909087860</identifier><identifier>PMID: 2707187</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Cells, Cultured ; Chromatography, Gel ; Cricetinae ; Epithelial Cells ; In Vitro Techniques ; Lipids - analysis ; Lipids - secretion ; Male ; Mesocricetus ; Molecular Weight ; Mucins - analysis ; Mucins - biosynthesis ; Mucins - secretion ; Proteoglycans - analysis ; Proteoglycans - biosynthesis ; Proteoglycans - secretion ; Trachea - analysis ; Trachea - cytology ; Trachea - secretion</subject><ispartof>Experimental lung research, 1989-01, Vol.15 (2), p.299-314</ispartof><rights>1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1989</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-ad4dd863a29d66900c4bbdd90b50711bb97a4f24f92c63069b58015a9e7aff23</citedby><cites>FETCH-LOGICAL-c401t-ad4dd863a29d66900c4bbdd90b50711bb97a4f24f92c63069b58015a9e7aff23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/01902148909087860$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/01902148909087860$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,59620,60409,61194,61375</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2707187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Kwang C.</creatorcontrib><creatorcontrib>Opaskar-Hincman, Helen</creatorcontrib><creatorcontrib>Bhaskar, K. Ramakrishnan</creatorcontrib><title>Secretions from Primary Hamster Tracheal Surface Epithelial Cells in Culture: Mucin-Like Glycoproteins, Proteoglycans, and Lipids</title><title>Experimental lung research</title><addtitle>Exp Lung Res</addtitle><description>Surface epithelial cells dissociated from hamster tracheas and grown on a thick collagen gel in the presence of 5% fetal bovine serum become highly enriched with secretory cells at confluence. In the present communication, we have analyzed secretory products from this primary hamster tracheal surface epithelial (HTSE) cell culture. The secreted glycoconjugates included high-molecular-weight mucin-like glycoproteins (HMW MLGP) and proteoglycans that comprised 22% and 5% of the total fHJglycoconjugates secreted when [3H]glucosamine was added as a metabolic precursor. Among the proteoglycans were hyaluronic acids (53%), heparan sulfate proteoglycans (29%), and chron-droitin sulfate proteoglycans (18%). Chondroitin sulfates were mostly 4-sulfated. On the other hand, the secreted lipids included cholesterol, phospholipids, and glycolipids, and most of them were associated with HMW MLGP.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Chromatography, Gel</subject><subject>Cricetinae</subject><subject>Epithelial Cells</subject><subject>In Vitro Techniques</subject><subject>Lipids - analysis</subject><subject>Lipids - secretion</subject><subject>Male</subject><subject>Mesocricetus</subject><subject>Molecular Weight</subject><subject>Mucins - analysis</subject><subject>Mucins - biosynthesis</subject><subject>Mucins - secretion</subject><subject>Proteoglycans - analysis</subject><subject>Proteoglycans - biosynthesis</subject><subject>Proteoglycans - secretion</subject><subject>Trachea - analysis</subject><subject>Trachea - cytology</subject><subject>Trachea - secretion</subject><issn>0190-2148</issn><issn>1521-0499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UUuLFDEQDuKyjqs_wIOQkyfbrfQ7uhcZ1t2FEYWde1Odh5M1nYxJGpmj_9w0Mwgi7imV-h5UfUXIKwbvKgb8EhiHktU9Bw5917fwhKxYU7ICas6fktWCFwvhGXke4wMAlE3fnpPzsoOO9d2K_LpXIqhkvItUBz_Rr8FMGA70FqeYVKDbgGKn0NL7OWgUil7vTdopa3JrrayN1Di6nm2ag3pPP8_CuGJjvit6Yw_C74NPyrj4Nvvmyn_LTVy-6CTdmL2R8QU502ijenl6L8j20_V2fVtsvtzcrT9uClEDSwXKWsq-rbDksm05gKjHUUoOY5NXYePIO6x1WWteiraClo9ND6xBrjrUuqwuyJujbR7px6xiGiYTRV4AnfJzHLqes5q1VSayI1EEH2NQetgfIxkYDEvqwz-pZ83rk_k8Tkr-UZxizvjVETdO-zDhTx-sHBIerA86oBMmLtb_t__wl3y5R9oJDGp48HNwObZHhvsNMumjxQ</recordid><startdate>19890101</startdate><enddate>19890101</enddate><creator>Kim, Kwang C.</creator><creator>Opaskar-Hincman, Helen</creator><creator>Bhaskar, K. Ramakrishnan</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19890101</creationdate><title>Secretions from Primary Hamster Tracheal Surface Epithelial Cells in Culture: Mucin-Like Glycoproteins, Proteoglycans, and Lipids</title><author>Kim, Kwang C. ; Opaskar-Hincman, Helen ; Bhaskar, K. Ramakrishnan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-ad4dd863a29d66900c4bbdd90b50711bb97a4f24f92c63069b58015a9e7aff23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Chromatography, Gel</topic><topic>Cricetinae</topic><topic>Epithelial Cells</topic><topic>In Vitro Techniques</topic><topic>Lipids - analysis</topic><topic>Lipids - secretion</topic><topic>Male</topic><topic>Mesocricetus</topic><topic>Molecular Weight</topic><topic>Mucins - analysis</topic><topic>Mucins - biosynthesis</topic><topic>Mucins - secretion</topic><topic>Proteoglycans - analysis</topic><topic>Proteoglycans - biosynthesis</topic><topic>Proteoglycans - secretion</topic><topic>Trachea - analysis</topic><topic>Trachea - cytology</topic><topic>Trachea - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Kwang C.</creatorcontrib><creatorcontrib>Opaskar-Hincman, Helen</creatorcontrib><creatorcontrib>Bhaskar, K. Ramakrishnan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental lung research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Kwang C.</au><au>Opaskar-Hincman, Helen</au><au>Bhaskar, K. Ramakrishnan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Secretions from Primary Hamster Tracheal Surface Epithelial Cells in Culture: Mucin-Like Glycoproteins, Proteoglycans, and Lipids</atitle><jtitle>Experimental lung research</jtitle><addtitle>Exp Lung Res</addtitle><date>1989-01-01</date><risdate>1989</risdate><volume>15</volume><issue>2</issue><spage>299</spage><epage>314</epage><pages>299-314</pages><issn>0190-2148</issn><eissn>1521-0499</eissn><abstract>Surface epithelial cells dissociated from hamster tracheas and grown on a thick collagen gel in the presence of 5% fetal bovine serum become highly enriched with secretory cells at confluence. In the present communication, we have analyzed secretory products from this primary hamster tracheal surface epithelial (HTSE) cell culture. The secreted glycoconjugates included high-molecular-weight mucin-like glycoproteins (HMW MLGP) and proteoglycans that comprised 22% and 5% of the total fHJglycoconjugates secreted when [3H]glucosamine was added as a metabolic precursor. Among the proteoglycans were hyaluronic acids (53%), heparan sulfate proteoglycans (29%), and chron-droitin sulfate proteoglycans (18%). Chondroitin sulfates were mostly 4-sulfated. On the other hand, the secreted lipids included cholesterol, phospholipids, and glycolipids, and most of them were associated with HMW MLGP.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>2707187</pmid><doi>10.3109/01902148909087860</doi><tpages>16</tpages></addata></record> |
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| identifier | ISSN: 0190-2148 |
| ispartof | Experimental lung research, 1989-01, Vol.15 (2), p.299-314 |
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| language | eng |
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| source | Taylor & Francis:Master (3349 titles); MEDLINE |
| subjects | Animals Cells, Cultured Chromatography, Gel Cricetinae Epithelial Cells In Vitro Techniques Lipids - analysis Lipids - secretion Male Mesocricetus Molecular Weight Mucins - analysis Mucins - biosynthesis Mucins - secretion Proteoglycans - analysis Proteoglycans - biosynthesis Proteoglycans - secretion Trachea - analysis Trachea - cytology Trachea - secretion |
| title | Secretions from Primary Hamster Tracheal Surface Epithelial Cells in Culture: Mucin-Like Glycoproteins, Proteoglycans, and Lipids |
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