Salicylhydroxamic acid enhances the NADH-oxidase activity of peroxidase in pea mitochondrial and chloroplast suspensions
Salicylhydroxamic acid (SHAM), an alternative oxidase inhibitor of plant mitochondria, enhances the NADH-oxidase activity in mitochondrial and chloroplast suspensions obtained from pea roots or leaves, respectively. This reaction is inhibited by the washing of mitochondria or chloroplasts and is obs...
Gespeichert in:
| Veröffentlicht in: | Moscow University biological sciences bulletin 2016, Vol.71 (1), p.19-23 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 23 |
|---|---|
| container_issue | 1 |
| container_start_page | 19 |
| container_title | Moscow University biological sciences bulletin |
| container_volume | 71 |
| creator | Samuilov, V. D D. B. Kiselevsky |
| description | Salicylhydroxamic acid (SHAM), an alternative oxidase inhibitor of plant mitochondria, enhances the NADH-oxidase activity in mitochondrial and chloroplast suspensions obtained from pea roots or leaves, respectively. This reaction is inhibited by the washing of mitochondria or chloroplasts and is observed in supernatants after the removal of the organelles by centrifugation. The reaction is sensitive to CNâ and to antioxidant propyl gallate. The NADH oxidation is also enhanced by 2,4-dichlorophenol or phenol, but not salicylic acid. The acceleration of NADH oxidation by phenolic compounds is observed with presence of commercial horseradish peroxidase and is connected with the involvement of these compounds in NADH-dependent peroxidase reaction. SHAM and 2,4-dichlorophenol significantly enhance the destruction of nuclei in guard cells of pea leaf epidermis caused by the generation of reactive oxygen species during the oxidation of exogenous NADH by apoplastic peroxidase. |
| doi_str_mv | 10.3103/S0096392516010089 |
| format | Article |
| fullrecord | <record><control><sourceid>fao_cross</sourceid><recordid>TN_cdi_crossref_primary_10_3103_S0096392516010089</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US201600196239</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2279-9ac71188a3b72b94c14fdd425513e7d7c5ac2c6b435af0e00c2d69b9482a00163</originalsourceid><addsrcrecordid>eNp9kEtLAzEUhYMoWKs_wJX5A6N5zCvLUh8Vii5qwd1wm2Q6KdNkSKYy8-9Nqa4EV5d7z_kuh4PQLSX3nBL-sCJE5FywjOaEElKKMzShgqdJIejnOZoc5eSoX6KrEHaEZJngYoKGFbRGjm0zKu8G2BuJQRqFtW3ASh1w32j8NntcJG4wCoKOcm--TD9iV-NO-9-zsXEDvDe9k42zyhtoMViFZdM677oWQo_DIXTaBuNsuEYXNbRB3_zMKVo_P33MF8ny_eV1PlsmkrFCJAJkQWlZAt8UbCNSSdNaqZRlGeW6UIXMQDKZb1KeQU00IZKpXERjyYAQmvMpoqe_0rsQvK6rzps9-LGipDpWV_2pLjLsxITotVvtq507eBtj_gvdnaAaXAVbb0K1XrEYIcYQOeOCfwM363vZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Salicylhydroxamic acid enhances the NADH-oxidase activity of peroxidase in pea mitochondrial and chloroplast suspensions</title><source>SpringerNature Journals</source><creator>Samuilov, V. D ; D. B. Kiselevsky</creator><creatorcontrib>Samuilov, V. D ; D. B. Kiselevsky</creatorcontrib><description>Salicylhydroxamic acid (SHAM), an alternative oxidase inhibitor of plant mitochondria, enhances the NADH-oxidase activity in mitochondrial and chloroplast suspensions obtained from pea roots or leaves, respectively. This reaction is inhibited by the washing of mitochondria or chloroplasts and is observed in supernatants after the removal of the organelles by centrifugation. The reaction is sensitive to CNâ and to antioxidant propyl gallate. The NADH oxidation is also enhanced by 2,4-dichlorophenol or phenol, but not salicylic acid. The acceleration of NADH oxidation by phenolic compounds is observed with presence of commercial horseradish peroxidase and is connected with the involvement of these compounds in NADH-dependent peroxidase reaction. SHAM and 2,4-dichlorophenol significantly enhance the destruction of nuclei in guard cells of pea leaf epidermis caused by the generation of reactive oxygen species during the oxidation of exogenous NADH by apoplastic peroxidase.</description><identifier>ISSN: 0096-3925</identifier><identifier>EISSN: 1934-791X</identifier><identifier>DOI: 10.3103/S0096392516010089</identifier><language>eng</language><publisher>New York: Allerton Press</publisher><subject>2,4-dichlorophenol ; antioxidants ; Biochemistry ; Biomedical and Life Sciences ; Cell Biology ; centrifugation ; chloroplasts ; guard cells ; leaves ; Life Sciences ; mitochondria ; NAD (coenzyme) ; oxidation ; peas ; peroxidase ; phenol ; Pisum sativum ; Plant Sciences ; propyl gallate ; reactive oxygen species ; roots ; salicylic acid ; washing ; Zoology</subject><ispartof>Moscow University biological sciences bulletin, 2016, Vol.71 (1), p.19-23</ispartof><rights>Allerton Press, Inc. 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2279-9ac71188a3b72b94c14fdd425513e7d7c5ac2c6b435af0e00c2d69b9482a00163</citedby><cites>FETCH-LOGICAL-c2279-9ac71188a3b72b94c14fdd425513e7d7c5ac2c6b435af0e00c2d69b9482a00163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S0096392516010089$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S0096392516010089$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Samuilov, V. D</creatorcontrib><creatorcontrib>D. B. Kiselevsky</creatorcontrib><title>Salicylhydroxamic acid enhances the NADH-oxidase activity of peroxidase in pea mitochondrial and chloroplast suspensions</title><title>Moscow University biological sciences bulletin</title><addtitle>Moscow Univ. Biol.Sci. Bull</addtitle><description>Salicylhydroxamic acid (SHAM), an alternative oxidase inhibitor of plant mitochondria, enhances the NADH-oxidase activity in mitochondrial and chloroplast suspensions obtained from pea roots or leaves, respectively. This reaction is inhibited by the washing of mitochondria or chloroplasts and is observed in supernatants after the removal of the organelles by centrifugation. The reaction is sensitive to CNâ and to antioxidant propyl gallate. The NADH oxidation is also enhanced by 2,4-dichlorophenol or phenol, but not salicylic acid. The acceleration of NADH oxidation by phenolic compounds is observed with presence of commercial horseradish peroxidase and is connected with the involvement of these compounds in NADH-dependent peroxidase reaction. SHAM and 2,4-dichlorophenol significantly enhance the destruction of nuclei in guard cells of pea leaf epidermis caused by the generation of reactive oxygen species during the oxidation of exogenous NADH by apoplastic peroxidase.</description><subject>2,4-dichlorophenol</subject><subject>antioxidants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>centrifugation</subject><subject>chloroplasts</subject><subject>guard cells</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>mitochondria</subject><subject>NAD (coenzyme)</subject><subject>oxidation</subject><subject>peas</subject><subject>peroxidase</subject><subject>phenol</subject><subject>Pisum sativum</subject><subject>Plant Sciences</subject><subject>propyl gallate</subject><subject>reactive oxygen species</subject><subject>roots</subject><subject>salicylic acid</subject><subject>washing</subject><subject>Zoology</subject><issn>0096-3925</issn><issn>1934-791X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLAzEUhYMoWKs_wJX5A6N5zCvLUh8Vii5qwd1wm2Q6KdNkSKYy8-9Nqa4EV5d7z_kuh4PQLSX3nBL-sCJE5FywjOaEElKKMzShgqdJIejnOZoc5eSoX6KrEHaEZJngYoKGFbRGjm0zKu8G2BuJQRqFtW3ASh1w32j8NntcJG4wCoKOcm--TD9iV-NO-9-zsXEDvDe9k42zyhtoMViFZdM677oWQo_DIXTaBuNsuEYXNbRB3_zMKVo_P33MF8ny_eV1PlsmkrFCJAJkQWlZAt8UbCNSSdNaqZRlGeW6UIXMQDKZb1KeQU00IZKpXERjyYAQmvMpoqe_0rsQvK6rzps9-LGipDpWV_2pLjLsxITotVvtq507eBtj_gvdnaAaXAVbb0K1XrEYIcYQOeOCfwM363vZ</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Samuilov, V. D</creator><creator>D. B. Kiselevsky</creator><general>Allerton Press</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2016</creationdate><title>Salicylhydroxamic acid enhances the NADH-oxidase activity of peroxidase in pea mitochondrial and chloroplast suspensions</title><author>Samuilov, V. D ; D. B. Kiselevsky</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2279-9ac71188a3b72b94c14fdd425513e7d7c5ac2c6b435af0e00c2d69b9482a00163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>2,4-dichlorophenol</topic><topic>antioxidants</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>centrifugation</topic><topic>chloroplasts</topic><topic>guard cells</topic><topic>leaves</topic><topic>Life Sciences</topic><topic>mitochondria</topic><topic>NAD (coenzyme)</topic><topic>oxidation</topic><topic>peas</topic><topic>peroxidase</topic><topic>phenol</topic><topic>Pisum sativum</topic><topic>Plant Sciences</topic><topic>propyl gallate</topic><topic>reactive oxygen species</topic><topic>roots</topic><topic>salicylic acid</topic><topic>washing</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samuilov, V. D</creatorcontrib><creatorcontrib>D. B. Kiselevsky</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Moscow University biological sciences bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Samuilov, V. D</au><au>D. B. Kiselevsky</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salicylhydroxamic acid enhances the NADH-oxidase activity of peroxidase in pea mitochondrial and chloroplast suspensions</atitle><jtitle>Moscow University biological sciences bulletin</jtitle><stitle>Moscow Univ. Biol.Sci. Bull</stitle><date>2016</date><risdate>2016</risdate><volume>71</volume><issue>1</issue><spage>19</spage><epage>23</epage><pages>19-23</pages><issn>0096-3925</issn><eissn>1934-791X</eissn><abstract>Salicylhydroxamic acid (SHAM), an alternative oxidase inhibitor of plant mitochondria, enhances the NADH-oxidase activity in mitochondrial and chloroplast suspensions obtained from pea roots or leaves, respectively. This reaction is inhibited by the washing of mitochondria or chloroplasts and is observed in supernatants after the removal of the organelles by centrifugation. The reaction is sensitive to CNâ and to antioxidant propyl gallate. The NADH oxidation is also enhanced by 2,4-dichlorophenol or phenol, but not salicylic acid. The acceleration of NADH oxidation by phenolic compounds is observed with presence of commercial horseradish peroxidase and is connected with the involvement of these compounds in NADH-dependent peroxidase reaction. SHAM and 2,4-dichlorophenol significantly enhance the destruction of nuclei in guard cells of pea leaf epidermis caused by the generation of reactive oxygen species during the oxidation of exogenous NADH by apoplastic peroxidase.</abstract><cop>New York</cop><pub>Allerton Press</pub><doi>10.3103/S0096392516010089</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0096-3925 |
| ispartof | Moscow University biological sciences bulletin, 2016, Vol.71 (1), p.19-23 |
| issn | 0096-3925 1934-791X |
| language | eng |
| recordid | cdi_crossref_primary_10_3103_S0096392516010089 |
| source | SpringerNature Journals |
| subjects | 2,4-dichlorophenol antioxidants Biochemistry Biomedical and Life Sciences Cell Biology centrifugation chloroplasts guard cells leaves Life Sciences mitochondria NAD (coenzyme) oxidation peas peroxidase phenol Pisum sativum Plant Sciences propyl gallate reactive oxygen species roots salicylic acid washing Zoology |
| title | Salicylhydroxamic acid enhances the NADH-oxidase activity of peroxidase in pea mitochondrial and chloroplast suspensions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T16%3A53%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Salicylhydroxamic%20acid%20enhances%20the%20NADH-oxidase%20activity%20of%20peroxidase%20in%20pea%20mitochondrial%20and%20chloroplast%20suspensions&rft.jtitle=Moscow%20University%20biological%20sciences%20bulletin&rft.au=Samuilov,%20V.%20D&rft.date=2016&rft.volume=71&rft.issue=1&rft.spage=19&rft.epage=23&rft.pages=19-23&rft.issn=0096-3925&rft.eissn=1934-791X&rft_id=info:doi/10.3103/S0096392516010089&rft_dat=%3Cfao_cross%3EUS201600196239%3C/fao_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |