Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi
Transgenic plants containing either ifn -α2b gene encoding human leukocytic interferon or esxA ::fbpB Δ TMD genes encoding Mycobacterium tuberculosis antigens ESAT6 and Ag85B were regenerated from hairy root cultures after transformation of chicory cotyledons ( Cichorium intybus L. var. Foliosum Heg...
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| Veröffentlicht in: | Cytology and genetics 2011-10, Vol.45 (5), p.277-281 |
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| container_title | Cytology and genetics |
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| creator | Matvieieva, N. A. Kishchenko, O. M. Potrochov, A. O. Shakhovsky, A. M. Kuchuk, M. V. |
| description | Transgenic plants containing either
ifn
-α2b gene encoding human leukocytic interferon or
esxA
::fbpB
Δ
TMD
genes encoding
Mycobacterium tuberculosis
antigens ESAT6 and Ag85B were regenerated from hairy root cultures after transformation of chicory cotyledons (
Cichorium intybus
L. var.
Foliosum Hegi
) with a wild-type
A. rhizogenes
A4 strain. The direct shoot regeneration from transgenic roots without callus formation phase on growth regulator-free nutrient medium was demonstrated. The transgenes transfer and transcription in the plants were confirmed by the results of RT-PCR and PCR analyses. |
| doi_str_mv | 10.3103/S0095452711050082 |
| format | Article |
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ifn
-α2b gene encoding human leukocytic interferon or
esxA
::fbpB
Δ
TMD
genes encoding
Mycobacterium tuberculosis
antigens ESAT6 and Ag85B were regenerated from hairy root cultures after transformation of chicory cotyledons (
Cichorium intybus
L. var.
Foliosum Hegi
) with a wild-type
A. rhizogenes
A4 strain. The direct shoot regeneration from transgenic roots without callus formation phase on growth regulator-free nutrient medium was demonstrated. The transgenes transfer and transcription in the plants were confirmed by the results of RT-PCR and PCR analyses.</description><identifier>ISSN: 0095-4527</identifier><identifier>EISSN: 1934-9440</identifier><identifier>DOI: 10.3103/S0095452711050082</identifier><language>eng</language><publisher>Heidelberg: Allerton Press, Inc</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Human Genetics</subject><ispartof>Cytology and genetics, 2011-10, Vol.45 (5), p.277-281</ispartof><rights>Allerton Press, Inc. 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-b267a38792540148ec0f7c6b3ec6f5c18c9fb60dfaf807bfac77b30953f59ac13</citedby><cites>FETCH-LOGICAL-c288t-b267a38792540148ec0f7c6b3ec6f5c18c9fb60dfaf807bfac77b30953f59ac13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S0095452711050082$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S0095452711050082$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Matvieieva, N. A.</creatorcontrib><creatorcontrib>Kishchenko, O. M.</creatorcontrib><creatorcontrib>Potrochov, A. O.</creatorcontrib><creatorcontrib>Shakhovsky, A. M.</creatorcontrib><creatorcontrib>Kuchuk, M. V.</creatorcontrib><title>Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi</title><title>Cytology and genetics</title><addtitle>Cytol. Genet</addtitle><description>Transgenic plants containing either
ifn
-α2b gene encoding human leukocytic interferon or
esxA
::fbpB
Δ
TMD
genes encoding
Mycobacterium tuberculosis
antigens ESAT6 and Ag85B were regenerated from hairy root cultures after transformation of chicory cotyledons (
Cichorium intybus
L. var.
Foliosum Hegi
) with a wild-type
A. rhizogenes
A4 strain. The direct shoot regeneration from transgenic roots without callus formation phase on growth regulator-free nutrient medium was demonstrated. The transgenes transfer and transcription in the plants were confirmed by the results of RT-PCR and PCR analyses.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Human Genetics</subject><issn>0095-4527</issn><issn>1934-9440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kN1KAzEQhYMoWKsP4F1eYOsk2Wyyl1KsLRQEfy5lycakTWmTMtkKfXtT6p3g1cD5zhlmDiH3DCaCgXh4A2hlLbliDCSA5hdkxFpRV21dwyUZnXB14tfkJucNgGy4ECPy-epWLjo0Q0iRJk8HNDEXKVi635o4ZOox7ejaBDxSTKkIxTUNdp0wHHY0xOHYHzJdTui3wQmdpW1IuYC5W4VbcuXNNru73zkmH7On9-m8Wr48L6aPy8pyrYeq540yQquWyxpYrZ0Fr2zTC2cbLy3TtvV9A1_eeA2q98Yq1YvykfCyNZaJMWHnvRZTzuh8t8ewM3jsGHSnfro__ZQMP2dy8caVw26TDhjLmf-EfgC5aGgl</recordid><startdate>20111001</startdate><enddate>20111001</enddate><creator>Matvieieva, N. A.</creator><creator>Kishchenko, O. M.</creator><creator>Potrochov, A. O.</creator><creator>Shakhovsky, A. M.</creator><creator>Kuchuk, M. V.</creator><general>Allerton Press, Inc</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20111001</creationdate><title>Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi</title><author>Matvieieva, N. A. ; Kishchenko, O. M. ; Potrochov, A. O. ; Shakhovsky, A. M. ; Kuchuk, M. V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-b267a38792540148ec0f7c6b3ec6f5c18c9fb60dfaf807bfac77b30953f59ac13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Human Genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matvieieva, N. A.</creatorcontrib><creatorcontrib>Kishchenko, O. M.</creatorcontrib><creatorcontrib>Potrochov, A. O.</creatorcontrib><creatorcontrib>Shakhovsky, A. M.</creatorcontrib><creatorcontrib>Kuchuk, M. V.</creatorcontrib><collection>CrossRef</collection><jtitle>Cytology and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matvieieva, N. A.</au><au>Kishchenko, O. M.</au><au>Potrochov, A. O.</au><au>Shakhovsky, A. M.</au><au>Kuchuk, M. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi</atitle><jtitle>Cytology and genetics</jtitle><stitle>Cytol. Genet</stitle><date>2011-10-01</date><risdate>2011</risdate><volume>45</volume><issue>5</issue><spage>277</spage><epage>281</epage><pages>277-281</pages><issn>0095-4527</issn><eissn>1934-9440</eissn><abstract>Transgenic plants containing either
ifn
-α2b gene encoding human leukocytic interferon or
esxA
::fbpB
Δ
TMD
genes encoding
Mycobacterium tuberculosis
antigens ESAT6 and Ag85B were regenerated from hairy root cultures after transformation of chicory cotyledons (
Cichorium intybus
L. var.
Foliosum Hegi
) with a wild-type
A. rhizogenes
A4 strain. The direct shoot regeneration from transgenic roots without callus formation phase on growth regulator-free nutrient medium was demonstrated. The transgenes transfer and transcription in the plants were confirmed by the results of RT-PCR and PCR analyses.</abstract><cop>Heidelberg</cop><pub>Allerton Press, Inc</pub><doi>10.3103/S0095452711050082</doi><tpages>5</tpages></addata></record> |
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| ispartof | Cytology and genetics, 2011-10, Vol.45 (5), p.277-281 |
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| language | eng |
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| source | SpringerLink Journals |
| subjects | Biomedical and Life Sciences Biomedicine Human Genetics |
| title | Regeneration of transgenic plants from hairy roots of Cichorium intybus L. var. Foliosum Hegi |
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