Molecular Detection of wzx1 and wzy Genes in Multi Drugs Resistance E. coli Isolates

Molecular Detection of wzx1 and wzy Genes in Multi Drugs Resistance E. coli Isolates

Escherichia coli have been genetically changes in Iraqi environments, the present study was carried out to detection E coli isolates sero-group using o-antigens gene cluster, tow genes were amplified used multiplex PCR, the results of present study show that present isolates have more than one plasm...

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Veröffentlicht in:International Journal of Pharmaceutical Quality Assurance 2018-10, Vol.9 (3)
Hauptverfasser: Al-Terehi, Mona, Khazaal, Saba Saadoon, Muhammed, Haidar J, Behjet, Russul Hikmat
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container_title International Journal of Pharmaceutical Quality Assurance
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creator Al-Terehi, Mona
Khazaal, Saba Saadoon
Muhammed, Haidar J
Behjet, Russul Hikmat
description Escherichia coli have been genetically changes in Iraqi environments, the present study was carried out to detection E coli isolates sero-group using o-antigens gene cluster, tow genes were amplified used multiplex PCR, the results of present study show that present isolates have more than one plasmid in different size (500-700 bp). PCR results show about 100% of isolates were had a positive results of PCR products of O45 wzx1 gene (451 bp) while 25% of isolates were a positive PCR products of O45 wzy1 gene (255 bp) and about 25% of isolates have tow genes. A new finding in present study that 50% of isolates have other copy of O45 wzx1 gene, these results concluded that it may be important in Iraqi isolates identification and classification however we need more information about genes sequencing.
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title Molecular Detection of wzx1 and wzy Genes in Multi Drugs Resistance E. coli Isolates
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