Changes in Oxidative Stress Biomarker and Gene Expression Levels in Workers Exposed to Volatile Organic Compounds
Exposure to volatile organic compounds (VOCs) was known to result in immunologic, respiratory, carcinogenic, reproductive, neurologic, and cardiovascular effects. However, the mechanisms by which VOCs induce these adverse health effects are not well understood. To evaluate the change of oxidative st...
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| Veröffentlicht in: | Industrial Health 2011, Vol.49(1), pp.8-14 |
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| creator | KIM, Jin Hee MOON, Jae Yong PARK, Eun-Young LEE, Kwan-Hee HONG, Yun-Chul |
| description | Exposure to volatile organic compounds (VOCs) was known to result in immunologic, respiratory, carcinogenic, reproductive, neurologic, and cardiovascular effects. However, the mechanisms by which VOCs induce these adverse health effects are not well understood. To evaluate the change of oxidative stress biomarker and gene expression levels in workers exposed to VOCs, we obtained urine and blood samples from 21 subjects before and after occupational exposure to VOCs. We measured levels of muconic acid (MuA), hippuric acid (HA), mandelic acid (MaA), and methyl hippuric acid (MHA) as urinary exposure biomarkers for benzene, toluene, ethylbenzene, and xylene (collectively BTEX), and malondialdehyde (MDA) and 8-hydroxydeoxyguanine (8-OHdG) as oxidative stress biomarkers in all subjects. We also evaluated BTEX-mediated RNA expression using cDNA microarray in 14 subjects. HA and MHA levels were higher following occupational exposure to VOCs (p |
| doi_str_mv | 10.2486/indhealth.MS1112 |
| format | Article |
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However, the mechanisms by which VOCs induce these adverse health effects are not well understood. To evaluate the change of oxidative stress biomarker and gene expression levels in workers exposed to VOCs, we obtained urine and blood samples from 21 subjects before and after occupational exposure to VOCs. We measured levels of muconic acid (MuA), hippuric acid (HA), mandelic acid (MaA), and methyl hippuric acid (MHA) as urinary exposure biomarkers for benzene, toluene, ethylbenzene, and xylene (collectively BTEX), and malondialdehyde (MDA) and 8-hydroxydeoxyguanine (8-OHdG) as oxidative stress biomarkers in all subjects. We also evaluated BTEX-mediated RNA expression using cDNA microarray in 14 subjects. HA and MHA levels were higher following occupational exposure to VOCs (p<0.01). In the linear regression analysis, HA ratios of after- and before-exposure were found to be significantly associated with increase of MDA ratios of after- and before-exposure after controlling for age, body mass index, and smoking (β=0.06, p=0.031). Evaluation of the gene expressions by HA showed that 23 gene expressions were found to be significantly associated with HA levels after adjusting for age, body mass index, and smoking (p<0.001). In particular, expressions of ENO3 and CDNA FLJ39461 fis among the 23 genes were significantly associated with the change in MDA level (p<0.05). Our study results suggest that exposure to VOCs, specifically toluene, induces oxidative stress and various gene expression change of which some may be responsible for oxidative stress.</description><identifier>ISSN: 0019-8366</identifier><identifier>EISSN: 1880-8026</identifier><identifier>DOI: 10.2486/indhealth.MS1112</identifier><identifier>PMID: 20823639</identifier><language>eng</language><publisher>Japan: National Institute of Occupational Safety and Health</publisher><subject>Adult ; Benzene - adverse effects ; Benzene Derivatives - adverse effects ; Biomarkers - blood ; Biomarkers - urine ; Expression microarray ; Gene Expression - drug effects ; Guanine - analogs & derivatives ; Guanine - blood ; Guanine - urine ; Hippurates - blood ; Hippurates - urine ; Humans ; Male ; Malondialdehyde - blood ; Malondialdehyde - urine ; Mandelic Acids - blood ; Mandelic Acids - urine ; Middle Aged ; Occupational Exposure - adverse effects ; Oligonucleotide Array Sequence Analysis ; Oxidative stress ; Oxidative Stress - drug effects ; Oxidative Stress - genetics ; Sorbic Acid - analogs & derivatives ; Sorbic Acid - analysis ; Toluene ; Toluene - adverse effects ; Volatile organic compounds ; Volatile Organic Compounds - adverse effects ; Xylenes - adverse effects</subject><ispartof>Industrial Health, 2011, Vol.49(1), pp.8-14</ispartof><rights>2011 by National Institute of Occupational Safety and Health</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-ee23ee59d917c1ef023431e51f964b7f7ca30aed492debe4e7fc49f91cceecbd3</citedby><cites>FETCH-LOGICAL-c565t-ee23ee59d917c1ef023431e51f964b7f7ca30aed492debe4e7fc49f91cceecbd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20823639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIM, Jin Hee</creatorcontrib><creatorcontrib>MOON, Jae Yong</creatorcontrib><creatorcontrib>PARK, Eun-Young</creatorcontrib><creatorcontrib>LEE, Kwan-Hee</creatorcontrib><creatorcontrib>HONG, Yun-Chul</creatorcontrib><title>Changes in Oxidative Stress Biomarker and Gene Expression Levels in Workers Exposed to Volatile Organic Compounds</title><title>Industrial Health</title><addtitle>Ind Health</addtitle><description>Exposure to volatile organic compounds (VOCs) was known to result in immunologic, respiratory, carcinogenic, reproductive, neurologic, and cardiovascular effects. However, the mechanisms by which VOCs induce these adverse health effects are not well understood. To evaluate the change of oxidative stress biomarker and gene expression levels in workers exposed to VOCs, we obtained urine and blood samples from 21 subjects before and after occupational exposure to VOCs. We measured levels of muconic acid (MuA), hippuric acid (HA), mandelic acid (MaA), and methyl hippuric acid (MHA) as urinary exposure biomarkers for benzene, toluene, ethylbenzene, and xylene (collectively BTEX), and malondialdehyde (MDA) and 8-hydroxydeoxyguanine (8-OHdG) as oxidative stress biomarkers in all subjects. We also evaluated BTEX-mediated RNA expression using cDNA microarray in 14 subjects. HA and MHA levels were higher following occupational exposure to VOCs (p<0.01). In the linear regression analysis, HA ratios of after- and before-exposure were found to be significantly associated with increase of MDA ratios of after- and before-exposure after controlling for age, body mass index, and smoking (β=0.06, p=0.031). Evaluation of the gene expressions by HA showed that 23 gene expressions were found to be significantly associated with HA levels after adjusting for age, body mass index, and smoking (p<0.001). In particular, expressions of ENO3 and CDNA FLJ39461 fis among the 23 genes were significantly associated with the change in MDA level (p<0.05). Our study results suggest that exposure to VOCs, specifically toluene, induces oxidative stress and various gene expression change of which some may be responsible for oxidative stress.</description><subject>Adult</subject><subject>Benzene - adverse effects</subject><subject>Benzene Derivatives - adverse effects</subject><subject>Biomarkers - blood</subject><subject>Biomarkers - urine</subject><subject>Expression microarray</subject><subject>Gene Expression - drug effects</subject><subject>Guanine - analogs & derivatives</subject><subject>Guanine - blood</subject><subject>Guanine - urine</subject><subject>Hippurates - blood</subject><subject>Hippurates - urine</subject><subject>Humans</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>Malondialdehyde - urine</subject><subject>Mandelic Acids - blood</subject><subject>Mandelic Acids - urine</subject><subject>Middle Aged</subject><subject>Occupational Exposure - adverse effects</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxidative Stress - genetics</subject><subject>Sorbic Acid - analogs & derivatives</subject><subject>Sorbic Acid - analysis</subject><subject>Toluene</subject><subject>Toluene - adverse effects</subject><subject>Volatile organic compounds</subject><subject>Volatile Organic Compounds - adverse effects</subject><subject>Xylenes - adverse effects</subject><issn>0019-8366</issn><issn>1880-8026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMtOwzAQRS0EoqWwZ4X8Ayl2nJeXUNGCVNRFeSwjx540Lqld7LQqf09DIGIzs5hzrzQHoWtKxmGUJbfaqApE3VTj5yWlNDxBQ5plJMhImJyiISGUBxlLkgG68H5NCEtizs7RICRZyBLGh-hzUgmzAo-1wYuDVqLRe8DLxoH3-F7bjXAf4LAwCs_AAH44bNuTtgbPYQ_1T_DdtpBvj9aDwo3Fb7Y-VtWAF24ljJZ4YjdbuzPKX6KzUtQern73CL1OH14mj8F8MXua3M0DGSdxEwCEDCDmitNUUihJyCJGIaYlT6IiLVMpGBGgIh4qKCCCtJQRLzmVEkAWio0Q6Xqls947KPOt08d3vnJK8tZe3tvLO3vHyE0X2e6KDag-8KfrCEw7YO0bsYIeEK7RsoZ_jRHPaTu65h6QlXA5GPYNZ42Ksw</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>KIM, Jin Hee</creator><creator>MOON, Jae Yong</creator><creator>PARK, Eun-Young</creator><creator>LEE, Kwan-Hee</creator><creator>HONG, Yun-Chul</creator><general>National Institute of Occupational Safety and Health</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2011</creationdate><title>Changes in Oxidative Stress Biomarker and Gene Expression Levels in Workers Exposed to Volatile Organic Compounds</title><author>KIM, Jin Hee ; MOON, Jae Yong ; PARK, Eun-Young ; LEE, Kwan-Hee ; HONG, Yun-Chul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-ee23ee59d917c1ef023431e51f964b7f7ca30aed492debe4e7fc49f91cceecbd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Benzene - adverse effects</topic><topic>Benzene Derivatives - adverse effects</topic><topic>Biomarkers - blood</topic><topic>Biomarkers - urine</topic><topic>Expression microarray</topic><topic>Gene Expression - drug effects</topic><topic>Guanine - analogs & derivatives</topic><topic>Guanine - blood</topic><topic>Guanine - urine</topic><topic>Hippurates - blood</topic><topic>Hippurates - urine</topic><topic>Humans</topic><topic>Male</topic><topic>Malondialdehyde - blood</topic><topic>Malondialdehyde - urine</topic><topic>Mandelic Acids - blood</topic><topic>Mandelic Acids - urine</topic><topic>Middle Aged</topic><topic>Occupational Exposure - adverse effects</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Oxidative Stress - genetics</topic><topic>Sorbic Acid - analogs & derivatives</topic><topic>Sorbic Acid - analysis</topic><topic>Toluene</topic><topic>Toluene - adverse effects</topic><topic>Volatile organic compounds</topic><topic>Volatile Organic Compounds - adverse effects</topic><topic>Xylenes - adverse effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIM, Jin Hee</creatorcontrib><creatorcontrib>MOON, Jae Yong</creatorcontrib><creatorcontrib>PARK, Eun-Young</creatorcontrib><creatorcontrib>LEE, Kwan-Hee</creatorcontrib><creatorcontrib>HONG, Yun-Chul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Industrial Health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KIM, Jin Hee</au><au>MOON, Jae Yong</au><au>PARK, Eun-Young</au><au>LEE, Kwan-Hee</au><au>HONG, Yun-Chul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in Oxidative Stress Biomarker and Gene Expression Levels in Workers Exposed to Volatile Organic Compounds</atitle><jtitle>Industrial Health</jtitle><addtitle>Ind Health</addtitle><date>2011</date><risdate>2011</risdate><volume>49</volume><issue>1</issue><spage>8</spage><epage>14</epage><pages>8-14</pages><issn>0019-8366</issn><eissn>1880-8026</eissn><abstract>Exposure to volatile organic compounds (VOCs) was known to result in immunologic, respiratory, carcinogenic, reproductive, neurologic, and cardiovascular effects. However, the mechanisms by which VOCs induce these adverse health effects are not well understood. To evaluate the change of oxidative stress biomarker and gene expression levels in workers exposed to VOCs, we obtained urine and blood samples from 21 subjects before and after occupational exposure to VOCs. We measured levels of muconic acid (MuA), hippuric acid (HA), mandelic acid (MaA), and methyl hippuric acid (MHA) as urinary exposure biomarkers for benzene, toluene, ethylbenzene, and xylene (collectively BTEX), and malondialdehyde (MDA) and 8-hydroxydeoxyguanine (8-OHdG) as oxidative stress biomarkers in all subjects. We also evaluated BTEX-mediated RNA expression using cDNA microarray in 14 subjects. HA and MHA levels were higher following occupational exposure to VOCs (p<0.01). In the linear regression analysis, HA ratios of after- and before-exposure were found to be significantly associated with increase of MDA ratios of after- and before-exposure after controlling for age, body mass index, and smoking (β=0.06, p=0.031). Evaluation of the gene expressions by HA showed that 23 gene expressions were found to be significantly associated with HA levels after adjusting for age, body mass index, and smoking (p<0.001). In particular, expressions of ENO3 and CDNA FLJ39461 fis among the 23 genes were significantly associated with the change in MDA level (p<0.05). Our study results suggest that exposure to VOCs, specifically toluene, induces oxidative stress and various gene expression change of which some may be responsible for oxidative stress.</abstract><cop>Japan</cop><pub>National Institute of Occupational Safety and Health</pub><pmid>20823639</pmid><doi>10.2486/indhealth.MS1112</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Benzene - adverse effects Benzene Derivatives - adverse effects Biomarkers - blood Biomarkers - urine Expression microarray Gene Expression - drug effects Guanine - analogs & derivatives Guanine - blood Guanine - urine Hippurates - blood Hippurates - urine Humans Male Malondialdehyde - blood Malondialdehyde - urine Mandelic Acids - blood Mandelic Acids - urine Middle Aged Occupational Exposure - adverse effects Oligonucleotide Array Sequence Analysis Oxidative stress Oxidative Stress - drug effects Oxidative Stress - genetics Sorbic Acid - analogs & derivatives Sorbic Acid - analysis Toluene Toluene - adverse effects Volatile organic compounds Volatile Organic Compounds - adverse effects Xylenes - adverse effects |
| title | Changes in Oxidative Stress Biomarker and Gene Expression Levels in Workers Exposed to Volatile Organic Compounds |
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