Biological and Chemical Assessment of DNA/Chitosan Complex Film
Transparent DNA/chitosan complex film was prepared from DNA/chitosan complex powder via hydrothermal hot pressing. In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA...
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| Veröffentlicht in: | Journal of Hard Tissue Biology 2014, Vol.23(4), pp.399-406 |
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| creator | Inoue, Yusuke Kawaguchi, Minoru Masui, Ichiro Horibe, Harumi Rikimaru, Tetsuya Kuroki, Madoka Katsumata, Yuri Mori, Nana Hayakawa, Tohru Fukushima, Tadao |
| description | Transparent DNA/chitosan complex film was prepared from DNA/chitosan complex powder via hydrothermal hot pressing. In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA/chitosan complex film to determine whether this film has potential for use in membranes for bone tissue engineering. The binding ratio of DH to DNA/chitosan complex film was very close to the excision number of 3.7 for the binding of DH by native DNA. This indicated that DNA in the complex did not denature as a result of hot pressing. Apatite was formed on the surface of the film after immersion in simulated body fluid. However, quartz crystal microbalance analysis showed that protein absorption to the DNA/chitosan complex was very low. It was found that this film has an affinity for minerals rather than proteins. Although the film remained in an almost unchanged configuration at 21 days after subcutaneous implantation, the response of rat soft tissues to the film was mild. These results suggest that DNA/chitosan complex film has potential for use as a membrane in bone tissue engineering. |
| doi_str_mv | 10.2485/jhtb.23.399 |
| format | Article |
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In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA/chitosan complex film to determine whether this film has potential for use in membranes for bone tissue engineering. The binding ratio of DH to DNA/chitosan complex film was very close to the excision number of 3.7 for the binding of DH by native DNA. This indicated that DNA in the complex did not denature as a result of hot pressing. Apatite was formed on the surface of the film after immersion in simulated body fluid. However, quartz crystal microbalance analysis showed that protein absorption to the DNA/chitosan complex was very low. It was found that this film has an affinity for minerals rather than proteins. Although the film remained in an almost unchanged configuration at 21 days after subcutaneous implantation, the response of rat soft tissues to the film was mild. These results suggest that DNA/chitosan complex film has potential for use as a membrane in bone tissue engineering.</description><identifier>ISSN: 1341-7649</identifier><identifier>EISSN: 1880-828X</identifier><identifier>DOI: 10.2485/jhtb.23.399</identifier><language>eng</language><publisher>THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY</publisher><subject>Biodegradation ; DNA/chitosan complex ; Hydrothermal hot-pressing ; Protective membrane ; Protein binding</subject><ispartof>Journal of Hard Tissue Biology, 2014, Vol.23(4), pp.399-406</ispartof><rights>2014 by The Hard Tissue Biology Network Association(JHTBNet)</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-6007bfb9e9cb506d47f123ae2b498c369da01f2224f8734a9cd30daba7b94d463</citedby><cites>FETCH-LOGICAL-c563t-6007bfb9e9cb506d47f123ae2b498c369da01f2224f8734a9cd30daba7b94d463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Inoue, Yusuke</creatorcontrib><creatorcontrib>Kawaguchi, Minoru</creatorcontrib><creatorcontrib>Masui, Ichiro</creatorcontrib><creatorcontrib>Horibe, Harumi</creatorcontrib><creatorcontrib>Rikimaru, Tetsuya</creatorcontrib><creatorcontrib>Kuroki, Madoka</creatorcontrib><creatorcontrib>Katsumata, Yuri</creatorcontrib><creatorcontrib>Mori, Nana</creatorcontrib><creatorcontrib>Hayakawa, Tohru</creatorcontrib><creatorcontrib>Fukushima, Tadao</creatorcontrib><creatorcontrib>Oral Surgery Section</creatorcontrib><creatorcontrib>Department of Dental Hygiene</creatorcontrib><creatorcontrib>Department of Dental Engineering</creatorcontrib><creatorcontrib>Fukuoka College of Health Sciences</creatorcontrib><creatorcontrib>Tsurumi University School of Dental Medicine</creatorcontrib><creatorcontrib>Department of Odontology</creatorcontrib><creatorcontrib>Periodontology Section</creatorcontrib><creatorcontrib>Department of Oral and Maxillofacial Surgery</creatorcontrib><creatorcontrib>Biomaterials Section</creatorcontrib><creatorcontrib>Fukuoka Dental College</creatorcontrib><creatorcontrib>Center for Regenerative Medicine</creatorcontrib><title>Biological and Chemical Assessment of DNA/Chitosan Complex Film</title><title>Journal of Hard Tissue Biology</title><addtitle>J. Hard Tissue Biology.</addtitle><description>Transparent DNA/chitosan complex film was prepared from DNA/chitosan complex powder via hydrothermal hot pressing. In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA/chitosan complex film to determine whether this film has potential for use in membranes for bone tissue engineering. The binding ratio of DH to DNA/chitosan complex film was very close to the excision number of 3.7 for the binding of DH by native DNA. This indicated that DNA in the complex did not denature as a result of hot pressing. Apatite was formed on the surface of the film after immersion in simulated body fluid. However, quartz crystal microbalance analysis showed that protein absorption to the DNA/chitosan complex was very low. It was found that this film has an affinity for minerals rather than proteins. Although the film remained in an almost unchanged configuration at 21 days after subcutaneous implantation, the response of rat soft tissues to the film was mild. These results suggest that DNA/chitosan complex film has potential for use as a membrane in bone tissue engineering.</description><subject>Biodegradation</subject><subject>DNA/chitosan complex</subject><subject>Hydrothermal hot-pressing</subject><subject>Protective membrane</subject><subject>Protein binding</subject><issn>1341-7649</issn><issn>1880-828X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNo9j8tOwzAQRS0EEqWw4geyR2nHjzy8AZVAC1IFG5DYWY7tNI6SuMRGgr8noairmdHcuXcOQtcYFoTlybKpQ7kgdEE5P0EznOcQ5yT_OB17ynCcpYyfowvvG4AUZ5zP0N29da3bWSXbSPY6KmrT_Q0r7433nelD5Kro4WW1LGobnJd9VLhu35rvaG3b7hKdVbL15uq_ztH7-vGteIq3r5vnYrWNVZLSEKcAWVmV3HBVJpBqllWYUGlIyXiuaMq1BFwRQliVZ5RJrjQFLUuZlZxpltI5ujn4qsF5P5hK7AfbyeFHYBATu5jYBaFiZB_Vm4O6M3qicX1reyMa9zX045dCf7JaBzvqATMBQChMhQoYrwUwSAglHGfJ6HR7cGp8kDtzTJVDsKo1x1T2H31cqFoOwvT0F-jXebA</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Inoue, Yusuke</creator><creator>Kawaguchi, Minoru</creator><creator>Masui, Ichiro</creator><creator>Horibe, Harumi</creator><creator>Rikimaru, Tetsuya</creator><creator>Kuroki, Madoka</creator><creator>Katsumata, Yuri</creator><creator>Mori, Nana</creator><creator>Hayakawa, Tohru</creator><creator>Fukushima, Tadao</creator><general>THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY</general><general>The Society for Hard Tissue Regenerative Biology</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2014</creationdate><title>Biological and Chemical Assessment of DNA/Chitosan Complex Film</title><author>Inoue, Yusuke ; Kawaguchi, Minoru ; Masui, Ichiro ; Horibe, Harumi ; Rikimaru, Tetsuya ; Kuroki, Madoka ; Katsumata, Yuri ; Mori, Nana ; Hayakawa, Tohru ; Fukushima, Tadao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c563t-6007bfb9e9cb506d47f123ae2b498c369da01f2224f8734a9cd30daba7b94d463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biodegradation</topic><topic>DNA/chitosan complex</topic><topic>Hydrothermal hot-pressing</topic><topic>Protective membrane</topic><topic>Protein binding</topic><toplevel>online_resources</toplevel><creatorcontrib>Inoue, Yusuke</creatorcontrib><creatorcontrib>Kawaguchi, Minoru</creatorcontrib><creatorcontrib>Masui, Ichiro</creatorcontrib><creatorcontrib>Horibe, Harumi</creatorcontrib><creatorcontrib>Rikimaru, Tetsuya</creatorcontrib><creatorcontrib>Kuroki, Madoka</creatorcontrib><creatorcontrib>Katsumata, Yuri</creatorcontrib><creatorcontrib>Mori, Nana</creatorcontrib><creatorcontrib>Hayakawa, Tohru</creatorcontrib><creatorcontrib>Fukushima, Tadao</creatorcontrib><creatorcontrib>Oral Surgery Section</creatorcontrib><creatorcontrib>Department of Dental Hygiene</creatorcontrib><creatorcontrib>Department of Dental Engineering</creatorcontrib><creatorcontrib>Fukuoka College of Health Sciences</creatorcontrib><creatorcontrib>Tsurumi University School of Dental Medicine</creatorcontrib><creatorcontrib>Department of Odontology</creatorcontrib><creatorcontrib>Periodontology Section</creatorcontrib><creatorcontrib>Department of Oral and Maxillofacial Surgery</creatorcontrib><creatorcontrib>Biomaterials Section</creatorcontrib><creatorcontrib>Fukuoka Dental College</creatorcontrib><creatorcontrib>Center for Regenerative Medicine</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of Hard Tissue Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, Yusuke</au><au>Kawaguchi, Minoru</au><au>Masui, Ichiro</au><au>Horibe, Harumi</au><au>Rikimaru, Tetsuya</au><au>Kuroki, Madoka</au><au>Katsumata, Yuri</au><au>Mori, Nana</au><au>Hayakawa, Tohru</au><au>Fukushima, Tadao</au><aucorp>Oral Surgery Section</aucorp><aucorp>Department of Dental Hygiene</aucorp><aucorp>Department of Dental Engineering</aucorp><aucorp>Fukuoka College of Health Sciences</aucorp><aucorp>Tsurumi University School of Dental Medicine</aucorp><aucorp>Department of Odontology</aucorp><aucorp>Periodontology Section</aucorp><aucorp>Department of Oral and Maxillofacial Surgery</aucorp><aucorp>Biomaterials Section</aucorp><aucorp>Fukuoka Dental College</aucorp><aucorp>Center for Regenerative Medicine</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological and Chemical Assessment of DNA/Chitosan Complex Film</atitle><jtitle>Journal of Hard Tissue Biology</jtitle><addtitle>J. Hard Tissue Biology.</addtitle><date>2014</date><risdate>2014</risdate><volume>23</volume><issue>4</issue><spage>399</spage><epage>406</epage><pages>399-406</pages><issn>1341-7649</issn><eissn>1880-828X</eissn><abstract>Transparent DNA/chitosan complex film was prepared from DNA/chitosan complex powder via hydrothermal hot pressing. In this study, we investigated the binding of daunorubicin hydrochloride (DH), proteins adsorption (fibronectin and albumin), mineral deposition, and rat soft-tissue response to the DNA/chitosan complex film to determine whether this film has potential for use in membranes for bone tissue engineering. The binding ratio of DH to DNA/chitosan complex film was very close to the excision number of 3.7 for the binding of DH by native DNA. This indicated that DNA in the complex did not denature as a result of hot pressing. Apatite was formed on the surface of the film after immersion in simulated body fluid. However, quartz crystal microbalance analysis showed that protein absorption to the DNA/chitosan complex was very low. It was found that this film has an affinity for minerals rather than proteins. Although the film remained in an almost unchanged configuration at 21 days after subcutaneous implantation, the response of rat soft tissues to the film was mild. These results suggest that DNA/chitosan complex film has potential for use as a membrane in bone tissue engineering.</abstract><pub>THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY</pub><doi>10.2485/jhtb.23.399</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biodegradation DNA/chitosan complex Hydrothermal hot-pressing Protective membrane Protein binding |
| title | Biological and Chemical Assessment of DNA/Chitosan Complex Film |
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