Phospholipid Composition and Tumoricidal Function of Alveolar Macrophage in Bronchoalveolar Lavage Fluid of Primary Lung Cancer Patients

Objective: We analysed the phospholipid composition and tumoricidal function of alveolar macrophages in bronchoalveolar lavage fluid (BALF) obtained from primary lung cancer patients. Methods: Bronchoalveolar lavage (BAL) was performed in 10 patients with primary lung cancer, 10 patients with stable...

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Veröffentlicht in:	Haigan 2001/08/20, Vol.41(4), pp.287-292
Hauptverfasser:	Kisohara, Akira, Takahashi, Noriaki, Koya, Yoshiaki, Horie, Takashi
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Sprache:	jpn
Schlagworte:	Alveolar macrophage Cytotoxicity Lung cancer Phospholipid
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creator	Kisohara, Akira Takahashi, Noriaki Koya, Yoshiaki Horie, Takashi
description	Objective: We analysed the phospholipid composition and tumoricidal function of alveolar macrophages in bronchoalveolar lavage fluid (BALF) obtained from primary lung cancer patients. Methods: Bronchoalveolar lavage (BAL) was performed in 10 patients with primary lung cancer, 10 patients with stable idiopathic interstitial pneumonitis and 5 healthy volunteers. The total protein and phospholipid contents and phospholipid classes in BALF were analysed. Alveolar macrophages were obtained from BALF, and cytotoxic in vitro assay was performed using A549 tumor cells. Results: The contents of phospholipid and protein in BALF were higher in primary lung cancer patients than in the others (p< 0.05). Analysis of BALF phospholipid showed that phosphatidylcholine was lower and sphingomyelin was higher in lung cancer patients than in the others. The ratio of phosphatidylcholine/sphingomyelin in BALF of primary lung cancer patients was lower than those in the others (p
doi_str_mv	10.2482/haigan.41.287
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Methods: Bronchoalveolar lavage (BAL) was performed in 10 patients with primary lung cancer, 10 patients with stable idiopathic interstitial pneumonitis and 5 healthy volunteers. The total protein and phospholipid contents and phospholipid classes in BALF were analysed. Alveolar macrophages were obtained from BALF, and cytotoxic in vitro assay was performed using A549 tumor cells. Results: The contents of phospholipid and protein in BALF were higher in primary lung cancer patients than in the others (p< 0.05). Analysis of BALF phospholipid showed that phosphatidylcholine was lower and sphingomyelin was higher in lung cancer patients than in the others. The ratio of phosphatidylcholine/sphingomyelin in BALF of primary lung cancer patients was lower than those in the others (p<0.05). Cytotoxicity of alveolar macrophages in BALF of lung cancer patients was lower than that of healthy volunteers, and the production of TNF-α and IL-1 was higher in lung cancer. An inverse correlation was observed between the cytotoxicity of alveolar macrophages and the volume of sphingomyelin in BALF. Conclusion: These findings suggested that changes in the surfactant composition of BALF may relate to the function of alveolar macrophages.</description><identifier>ISSN: 0386-9628</identifier><identifier>EISSN: 1348-9992</identifier><identifier>DOI: 10.2482/haigan.41.287</identifier><language>jpn</language><publisher>The Japan Lung Cancer Society</publisher><subject>Alveolar macrophage ; Cytotoxicity ; Lung cancer ; Phospholipid</subject><ispartof>Haigan, 2001/08/20, Vol.41(4), pp.287-292</ispartof><rights>The Japan Lung Cancer Society</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Kisohara, Akira</creatorcontrib><creatorcontrib>Takahashi, Noriaki</creatorcontrib><creatorcontrib>Koya, Yoshiaki</creatorcontrib><creatorcontrib>Horie, Takashi</creatorcontrib><title>Phospholipid Composition and Tumoricidal Function of Alveolar Macrophage in Bronchoalveolar Lavage Fluid of Primary Lung Cancer Patients</title><title>Haigan</title><addtitle>JJLC</addtitle><description>Objective: We analysed the phospholipid composition and tumoricidal function of alveolar macrophages in bronchoalveolar lavage fluid (BALF) obtained from primary lung cancer patients. Methods: Bronchoalveolar lavage (BAL) was performed in 10 patients with primary lung cancer, 10 patients with stable idiopathic interstitial pneumonitis and 5 healthy volunteers. The total protein and phospholipid contents and phospholipid classes in BALF were analysed. Alveolar macrophages were obtained from BALF, and cytotoxic in vitro assay was performed using A549 tumor cells. Results: The contents of phospholipid and protein in BALF were higher in primary lung cancer patients than in the others (p< 0.05). Analysis of BALF phospholipid showed that phosphatidylcholine was lower and sphingomyelin was higher in lung cancer patients than in the others. The ratio of phosphatidylcholine/sphingomyelin in BALF of primary lung cancer patients was lower than those in the others (p<0.05). Cytotoxicity of alveolar macrophages in BALF of lung cancer patients was lower than that of healthy volunteers, and the production of TNF-α and IL-1 was higher in lung cancer. An inverse correlation was observed between the cytotoxicity of alveolar macrophages and the volume of sphingomyelin in BALF. Conclusion: These findings suggested that changes in the surfactant composition of BALF may relate to the function of alveolar macrophages.</description><subject>Alveolar macrophage</subject><subject>Cytotoxicity</subject><subject>Lung cancer</subject><subject>Phospholipid</subject><issn>0386-9628</issn><issn>1348-9992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNo9kMFOwzAQRC0EEqVw5O4fSIlj14mPJSKAFEQPvUcb22lcpXZkJ5X4Az6blBQuu4d5M9odhB5JvEpYljy1YPZgV4yskiy9QgtCWRYJIZJrtIhpxiPBk-wW3YVwiGOerDldoO9t60Lfus70RuHcHXsXzGCcxWAV3o1H5400CjpcjFb-Cq7Bm-6kXQcef4D0rm9hr7Gx-Nk7K1sHf2oJp7NSdOOUPdm23hzBf-FytHucg5Xa4y0MRtsh3KObBrqgHy57iXbFyy5_i8rP1_d8U0Yy4es0ojQVet00KUlqxZWGOCWkpkowQZgiJOaNTKnQoFhTZzKrGSjJuFDQ1IRJukTRHDvdHYLXTdXPR1Ukrs4tVnOLFSPV1OLEFzN_CMP0yz8NfjCy0xeaCB6fHWwek_EfkC34Slv6A0v9g2g</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Kisohara, Akira</creator><creator>Takahashi, Noriaki</creator><creator>Koya, Yoshiaki</creator><creator>Horie, Takashi</creator><general>The Japan Lung Cancer Society</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2001</creationdate><title>Phospholipid Composition and Tumoricidal Function of Alveolar Macrophage in Bronchoalveolar Lavage Fluid of Primary Lung Cancer Patients</title><author>Kisohara, Akira ; Takahashi, Noriaki ; Koya, Yoshiaki ; Horie, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2657-3379e5ff712bd6dea0711b3d94914d1106fc739ead4fb8c8b4adc469dafb14c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>jpn</language><creationdate>2001</creationdate><topic>Alveolar macrophage</topic><topic>Cytotoxicity</topic><topic>Lung cancer</topic><topic>Phospholipid</topic><toplevel>online_resources</toplevel><creatorcontrib>Kisohara, Akira</creatorcontrib><creatorcontrib>Takahashi, Noriaki</creatorcontrib><creatorcontrib>Koya, Yoshiaki</creatorcontrib><creatorcontrib>Horie, Takashi</creatorcontrib><collection>CrossRef</collection><jtitle>Haigan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kisohara, Akira</au><au>Takahashi, Noriaki</au><au>Koya, Yoshiaki</au><au>Horie, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phospholipid Composition and Tumoricidal Function of Alveolar Macrophage in Bronchoalveolar Lavage Fluid of Primary Lung Cancer Patients</atitle><jtitle>Haigan</jtitle><addtitle>JJLC</addtitle><date>2001</date><risdate>2001</risdate><volume>41</volume><issue>4</issue><spage>287</spage><epage>292</epage><pages>287-292</pages><issn>0386-9628</issn><eissn>1348-9992</eissn><abstract>Objective: We analysed the phospholipid composition and tumoricidal function of alveolar macrophages in bronchoalveolar lavage fluid (BALF) obtained from primary lung cancer patients. Methods: Bronchoalveolar lavage (BAL) was performed in 10 patients with primary lung cancer, 10 patients with stable idiopathic interstitial pneumonitis and 5 healthy volunteers. The total protein and phospholipid contents and phospholipid classes in BALF were analysed. Alveolar macrophages were obtained from BALF, and cytotoxic in vitro assay was performed using A549 tumor cells. Results: The contents of phospholipid and protein in BALF were higher in primary lung cancer patients than in the others (p< 0.05). Analysis of BALF phospholipid showed that phosphatidylcholine was lower and sphingomyelin was higher in lung cancer patients than in the others. The ratio of phosphatidylcholine/sphingomyelin in BALF of primary lung cancer patients was lower than those in the others (p<0.05). Cytotoxicity of alveolar macrophages in BALF of lung cancer patients was lower than that of healthy volunteers, and the production of TNF-α and IL-1 was higher in lung cancer. An inverse correlation was observed between the cytotoxicity of alveolar macrophages and the volume of sphingomyelin in BALF. Conclusion: These findings suggested that changes in the surfactant composition of BALF may relate to the function of alveolar macrophages.</abstract><pub>The Japan Lung Cancer Society</pub><doi>10.2482/haigan.41.287</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects	Alveolar macrophage Cytotoxicity Lung cancer Phospholipid
title	Phospholipid Composition and Tumoricidal Function of Alveolar Macrophage in Bronchoalveolar Lavage Fluid of Primary Lung Cancer Patients
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