DNA Sequencing Analysis of African Xanthomonas oryzae pv. oryzae Virulence Gene (AXaVrg) DNA Marker
Global rice production is constrained by bacterial leaf blight (BLB) disease caused by pv. ( ). BLB disease incidence in West Africa was between 70–85% and yield loss in farmers’ fields was in the range of 50–90% from 2005 to 2010. In the present study, African virulence gene OPP-17 DNA marker was i...
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| Veröffentlicht in: | Scientia agriculturae Bohemica 2018-06, Vol.49 (2), p.78-86 |
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| creator | Onasanya, A. Ekperigin, M.M. Onasanya, R.O. Obafemi, T.O. Ogundipe, A.T. Ojo, A.A. Ingelbrecht, I. |
| description | Global rice production is constrained by bacterial leaf blight (BLB) disease caused by
pv.
(
). BLB disease incidence in West Africa was between 70–85% and yield loss in farmers’ fields was in the range of 50–90% from 2005 to 2010. In the present study, African
virulence gene OPP-17
DNA marker was identified and purified using randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) products from 50
isolates. Genomic DNA of 50
isolates were analyzed using OPP-17 primer in RAPD-PCR during which African
virulence gene OPP-17
DNA marker was identified, purified, cloned, and sequenced. Cloning and DNA sequencing of African
virulence gene OPP-17
DNA generated a 1953 bp nucleotide sequence consequently tagged as
-1953. BLAST homologous analysis of the
1953 sequence provides comprehensive identification of the type II secretion genes and secreted proteins, type III secretion genes and secreted proteins in African
virulence gene. Phylogenetic unweighted pairgroup method arithmetic (UPGMA) analysis revealed the African
-1953 sequence was distinct from the other
virulence gene sequences from China, Japan, Korea, Germany, and the United States. This information is potentially useful for effective management of BLB disease in West Africa. |
| doi_str_mv | 10.2478/sab-2018-0012 |
| format | Article |
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pv.
(
). BLB disease incidence in West Africa was between 70–85% and yield loss in farmers’ fields was in the range of 50–90% from 2005 to 2010. In the present study, African
virulence gene OPP-17
DNA marker was identified and purified using randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) products from 50
isolates. Genomic DNA of 50
isolates were analyzed using OPP-17 primer in RAPD-PCR during which African
virulence gene OPP-17
DNA marker was identified, purified, cloned, and sequenced. Cloning and DNA sequencing of African
virulence gene OPP-17
DNA generated a 1953 bp nucleotide sequence consequently tagged as
-1953. BLAST homologous analysis of the
1953 sequence provides comprehensive identification of the type II secretion genes and secreted proteins, type III secretion genes and secreted proteins in African
virulence gene. Phylogenetic unweighted pairgroup method arithmetic (UPGMA) analysis revealed the African
-1953 sequence was distinct from the other
virulence gene sequences from China, Japan, Korea, Germany, and the United States. This information is potentially useful for effective management of BLB disease in West Africa.</description><identifier>ISSN: 1211-3174</identifier><identifier>EISSN: 1805-9430</identifier><identifier>DOI: 10.2478/sab-2018-0012</identifier><language>eng</language><publisher>Sciendo</publisher><subject>Bacterial leaf blight ; BLAST ; cloning ; Operon primer ; RAPD-PCR products ; Secreted proteins ; virulence gene DNA marker ; West Africa</subject><ispartof>Scientia agriculturae Bohemica, 2018-06, Vol.49 (2), p.78-86</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1988-b65c18995f20ceb8becf6b85b61aa8bf91afa3a3538ca77f327d979d9e12d0363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Onasanya, A.</creatorcontrib><creatorcontrib>Ekperigin, M.M.</creatorcontrib><creatorcontrib>Onasanya, R.O.</creatorcontrib><creatorcontrib>Obafemi, T.O.</creatorcontrib><creatorcontrib>Ogundipe, A.T.</creatorcontrib><creatorcontrib>Ojo, A.A.</creatorcontrib><creatorcontrib>Ingelbrecht, I.</creatorcontrib><title>DNA Sequencing Analysis of African Xanthomonas oryzae pv. oryzae Virulence Gene (AXaVrg) DNA Marker</title><title>Scientia agriculturae Bohemica</title><description>Global rice production is constrained by bacterial leaf blight (BLB) disease caused by
pv.
(
). BLB disease incidence in West Africa was between 70–85% and yield loss in farmers’ fields was in the range of 50–90% from 2005 to 2010. In the present study, African
virulence gene OPP-17
DNA marker was identified and purified using randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) products from 50
isolates. Genomic DNA of 50
isolates were analyzed using OPP-17 primer in RAPD-PCR during which African
virulence gene OPP-17
DNA marker was identified, purified, cloned, and sequenced. Cloning and DNA sequencing of African
virulence gene OPP-17
DNA generated a 1953 bp nucleotide sequence consequently tagged as
-1953. BLAST homologous analysis of the
1953 sequence provides comprehensive identification of the type II secretion genes and secreted proteins, type III secretion genes and secreted proteins in African
virulence gene. Phylogenetic unweighted pairgroup method arithmetic (UPGMA) analysis revealed the African
-1953 sequence was distinct from the other
virulence gene sequences from China, Japan, Korea, Germany, and the United States. This information is potentially useful for effective management of BLB disease in West Africa.</description><subject>Bacterial leaf blight</subject><subject>BLAST</subject><subject>cloning</subject><subject>Operon primer</subject><subject>RAPD-PCR products</subject><subject>Secreted proteins</subject><subject>virulence gene DNA marker</subject><subject>West Africa</subject><issn>1211-3174</issn><issn>1805-9430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNptkL1PwzAUxC0EElVhZPcIg4s_8mGzRQUKUoEBqLpFL45dUtKk2A0o_PU4Kmy85Z1Odzf8EDpjdMKjVF56KAinTBJKGT9AIyZpTFQk6GHQnDEiWBodo1Pv1zRcLKlgYoT09WOGn81HZxpdNSucNVD3vvK4tTizrtLQ4CU0u7d20zYQbNd_g8Hbz8mfXFSuq0Pb4JlpDD7PlrBwqws8DD-AezfuBB1ZqL05_f1j9Hp78zK9I_On2f00mxPNlJSkSGLNpFKx5VSbQhZG26SQcZEwAFlYxcCCABELqSFNreBpqVJVKsN4SUUixojsd7VrvXfG5ltXbcD1OaP5ACkPkPIBUj5ACvmrff4L6p1xpVm5rg8iX7edCxz8_71I8VSKH4x6bRo</recordid><startdate>20180601</startdate><enddate>20180601</enddate><creator>Onasanya, A.</creator><creator>Ekperigin, M.M.</creator><creator>Onasanya, R.O.</creator><creator>Obafemi, T.O.</creator><creator>Ogundipe, A.T.</creator><creator>Ojo, A.A.</creator><creator>Ingelbrecht, I.</creator><general>Sciendo</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180601</creationdate><title>DNA Sequencing Analysis of African Xanthomonas oryzae pv. oryzae Virulence Gene (AXaVrg) DNA Marker</title><author>Onasanya, A. ; Ekperigin, M.M. ; Onasanya, R.O. ; Obafemi, T.O. ; Ogundipe, A.T. ; Ojo, A.A. ; Ingelbrecht, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1988-b65c18995f20ceb8becf6b85b61aa8bf91afa3a3538ca77f327d979d9e12d0363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Bacterial leaf blight</topic><topic>BLAST</topic><topic>cloning</topic><topic>Operon primer</topic><topic>RAPD-PCR products</topic><topic>Secreted proteins</topic><topic>virulence gene DNA marker</topic><topic>West Africa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Onasanya, A.</creatorcontrib><creatorcontrib>Ekperigin, M.M.</creatorcontrib><creatorcontrib>Onasanya, R.O.</creatorcontrib><creatorcontrib>Obafemi, T.O.</creatorcontrib><creatorcontrib>Ogundipe, A.T.</creatorcontrib><creatorcontrib>Ojo, A.A.</creatorcontrib><creatorcontrib>Ingelbrecht, I.</creatorcontrib><collection>CrossRef</collection><jtitle>Scientia agriculturae Bohemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Onasanya, A.</au><au>Ekperigin, M.M.</au><au>Onasanya, R.O.</au><au>Obafemi, T.O.</au><au>Ogundipe, A.T.</au><au>Ojo, A.A.</au><au>Ingelbrecht, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Sequencing Analysis of African Xanthomonas oryzae pv. oryzae Virulence Gene (AXaVrg) DNA Marker</atitle><jtitle>Scientia agriculturae Bohemica</jtitle><date>2018-06-01</date><risdate>2018</risdate><volume>49</volume><issue>2</issue><spage>78</spage><epage>86</epage><pages>78-86</pages><issn>1211-3174</issn><eissn>1805-9430</eissn><abstract>Global rice production is constrained by bacterial leaf blight (BLB) disease caused by
pv.
(
). BLB disease incidence in West Africa was between 70–85% and yield loss in farmers’ fields was in the range of 50–90% from 2005 to 2010. In the present study, African
virulence gene OPP-17
DNA marker was identified and purified using randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) products from 50
isolates. Genomic DNA of 50
isolates were analyzed using OPP-17 primer in RAPD-PCR during which African
virulence gene OPP-17
DNA marker was identified, purified, cloned, and sequenced. Cloning and DNA sequencing of African
virulence gene OPP-17
DNA generated a 1953 bp nucleotide sequence consequently tagged as
-1953. BLAST homologous analysis of the
1953 sequence provides comprehensive identification of the type II secretion genes and secreted proteins, type III secretion genes and secreted proteins in African
virulence gene. Phylogenetic unweighted pairgroup method arithmetic (UPGMA) analysis revealed the African
-1953 sequence was distinct from the other
virulence gene sequences from China, Japan, Korea, Germany, and the United States. This information is potentially useful for effective management of BLB disease in West Africa.</abstract><pub>Sciendo</pub><doi>10.2478/sab-2018-0012</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1211-3174 1805-9430 |
| language | eng |
| recordid | cdi_crossref_primary_10_2478_sab_2018_0012 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Bacterial leaf blight BLAST cloning Operon primer RAPD-PCR products Secreted proteins virulence gene DNA marker West Africa |
| title | DNA Sequencing Analysis of African Xanthomonas oryzae pv. oryzae Virulence Gene (AXaVrg) DNA Marker |
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