Variation of Single-Strand Break Yield with Scavenger Concentration for Plasmid DNA Irradiated in Aqueous Solution
We have measured the yield of single-strand breaks (SSBs), induced by 137 Cs γ radiation as assayed by agarose gel electrophoresis, for three plasmids and SV40 DNA irradiated in aerobic aqueous solution. DNA SSBs are caused mainly by the hydroxyl radical under these conditions. To characterize the r...
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| Veröffentlicht in: | Radiation research 1993-02, Vol.133 (2), p.151-157 |
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| creator | Milligan, J. R. Aguilera, J. A. Ward, J. F. |
| description | We have measured the yield of single-strand breaks (SSBs), induced by 137 Cs γ radiation as assayed by agarose gel electrophoresis, for three plasmids and SV40 DNA irradiated in aerobic aqueous solution. DNA SSBs are caused mainly by the hydroxyl radical under these conditions. To characterize the reactivity of DNA with the hydroxyl radical, we investigated the variation of the G value for SSBs [G(SSB)] with the concentration of added hydroxyl radical scavengers. We find that simple competition kinetics does not describe our results, but that a nonhomogeneous kinetics model is in good agreement. At a DNA concentration of 50 μg cm-3, G(SSB) for the direct effect is about $1\times 10^{-5}\ \mu {\rm mol}\ {\rm J}^{-1}$ for the DNA substrates studied. This is equivalent to 2× 10-10 SSB ${\rm Gy}^{-1}\ {\rm Da}^{-1}$. Estimates of the efficiency of SSB induction per ${\rm OH}^{\bullet}$ radical interaction with DNA (0.32-0.44) reveal that all plasmids are essentially equal in reactivity. |
| doi_str_mv | 10.2307/3578350 |
| format | Article |
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R. ; Aguilera, J. A. ; Ward, J. F.</creator><creatorcontrib>Milligan, J. R. ; Aguilera, J. A. ; Ward, J. F.</creatorcontrib><description>We have measured the yield of single-strand breaks (SSBs), induced by 137 Cs γ radiation as assayed by agarose gel electrophoresis, for three plasmids and SV40 DNA irradiated in aerobic aqueous solution. DNA SSBs are caused mainly by the hydroxyl radical under these conditions. To characterize the reactivity of DNA with the hydroxyl radical, we investigated the variation of the G value for SSBs [G(SSB)] with the concentration of added hydroxyl radical scavengers. We find that simple competition kinetics does not describe our results, but that a nonhomogeneous kinetics model is in good agreement. At a DNA concentration of 50 μg cm-3, G(SSB) for the direct effect is about $1\times 10^{-5}\ \mu {\rm mol}\ {\rm J}^{-1}$ for the DNA substrates studied. This is equivalent to 2× 10-10 SSB ${\rm Gy}^{-1}\ {\rm Da}^{-1}$. Estimates of the efficiency of SSB induction per ${\rm OH}^{\bullet}$ radical interaction with DNA (0.32-0.44) reveal that all plasmids are essentially equal in reactivity.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3578350</identifier><identifier>PMID: 8382368</identifier><identifier>CODEN: RAREAE</identifier><language>eng</language><publisher>Oak Brook, Il: Academic Press, Inc</publisher><subject>Aqueous solutions ; Biological and medical sciences ; Biological effects of radiation ; Cesium Radioisotopes ; DNA ; DNA Damage ; DNA, Single-Stranded - radiation effects ; DNA, Viral - radiation effects ; Free Radical Scavengers ; Fundamental and applied biological sciences. Psychology ; Gamma Rays ; Gels ; Genetic variation ; Hydroxyl radicals ; Ionizing radiations ; Kinetics ; Plasmids ; Radiation dosage ; Radiation Genetics ; Radius of a sphere ; Scavenging ; Simian virus 40 ; Solutions ; Tissues, organs and organisms biophysics ; Water</subject><ispartof>Radiation research, 1993-02, Vol.133 (2), p.151-157</ispartof><rights>Copyright 1993 Academic Press, Inc.</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-f776c57520eb153fe94c43bb42d874c5cc2454db94b10befc8ea85f80ad77b0b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3578350$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3578350$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4681100$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8382368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Milligan, J. R.</creatorcontrib><creatorcontrib>Aguilera, J. A.</creatorcontrib><creatorcontrib>Ward, J. F.</creatorcontrib><title>Variation of Single-Strand Break Yield with Scavenger Concentration for Plasmid DNA Irradiated in Aqueous Solution</title><title>Radiation research</title><addtitle>Radiat Res</addtitle><description>We have measured the yield of single-strand breaks (SSBs), induced by 137 Cs γ radiation as assayed by agarose gel electrophoresis, for three plasmids and SV40 DNA irradiated in aerobic aqueous solution. DNA SSBs are caused mainly by the hydroxyl radical under these conditions. To characterize the reactivity of DNA with the hydroxyl radical, we investigated the variation of the G value for SSBs [G(SSB)] with the concentration of added hydroxyl radical scavengers. We find that simple competition kinetics does not describe our results, but that a nonhomogeneous kinetics model is in good agreement. At a DNA concentration of 50 μg cm-3, G(SSB) for the direct effect is about $1\times 10^{-5}\ \mu {\rm mol}\ {\rm J}^{-1}$ for the DNA substrates studied. This is equivalent to 2× 10-10 SSB ${\rm Gy}^{-1}\ {\rm Da}^{-1}$. Estimates of the efficiency of SSB induction per ${\rm OH}^{\bullet}$ radical interaction with DNA (0.32-0.44) reveal that all plasmids are essentially equal in reactivity.</description><subject>Aqueous solutions</subject><subject>Biological and medical sciences</subject><subject>Biological effects of radiation</subject><subject>Cesium Radioisotopes</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>DNA, Single-Stranded - radiation effects</subject><subject>DNA, Viral - radiation effects</subject><subject>Free Radical Scavengers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma Rays</subject><subject>Gels</subject><subject>Genetic variation</subject><subject>Hydroxyl radicals</subject><subject>Ionizing radiations</subject><subject>Kinetics</subject><subject>Plasmids</subject><subject>Radiation dosage</subject><subject>Radiation Genetics</subject><subject>Radius of a sphere</subject><subject>Scavenging</subject><subject>Simian virus 40</subject><subject>Solutions</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Water</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1LwzAUhoMoc07xFwi5ELyqpk3SpJdzfg2GClXBq5LPmdm1M2kV_72dK_PKq8Phfc574AHgOEbnCUbsAlPGMUU7YBhnmEeUILILhghhHDHK2T44CGGBuj1OswEYcMwTnPIh8C_CO9G4uoK1hbmr5qWJ8saLSsNLb8Q7fHWm1PDLNW8wV-LTVHPj4aSulKk67PfS1h4-liIsnYZX92M49V7ortVo6Co4_mhN3QaY12W7xg_BnhVlMEf9HIHnm-unyV00e7idTsazSGFMmsgylirKaIKMjCm2JiOKYClJojkjiiqVEEq0zIiMkTRWcSM4tRwJzZhEEo_A2aZX-ToEb2yx8m4p_HcRo2ItreildeTJhly1cmn0lustdflpn4ugRGk7O8qFLUZSHscI_WGL0NT-328_VXZ_ZQ</recordid><startdate>19930201</startdate><enddate>19930201</enddate><creator>Milligan, J. R.</creator><creator>Aguilera, J. A.</creator><creator>Ward, J. F.</creator><general>Academic Press, Inc</general><general>Radiation Research Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19930201</creationdate><title>Variation of Single-Strand Break Yield with Scavenger Concentration for Plasmid DNA Irradiated in Aqueous Solution</title><author>Milligan, J. R. ; Aguilera, J. A. ; Ward, J. F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-f776c57520eb153fe94c43bb42d874c5cc2454db94b10befc8ea85f80ad77b0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Aqueous solutions</topic><topic>Biological and medical sciences</topic><topic>Biological effects of radiation</topic><topic>Cesium Radioisotopes</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>DNA, Single-Stranded - radiation effects</topic><topic>DNA, Viral - radiation effects</topic><topic>Free Radical Scavengers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma Rays</topic><topic>Gels</topic><topic>Genetic variation</topic><topic>Hydroxyl radicals</topic><topic>Ionizing radiations</topic><topic>Kinetics</topic><topic>Plasmids</topic><topic>Radiation dosage</topic><topic>Radiation Genetics</topic><topic>Radius of a sphere</topic><topic>Scavenging</topic><topic>Simian virus 40</topic><topic>Solutions</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Milligan, J. R.</creatorcontrib><creatorcontrib>Aguilera, J. A.</creatorcontrib><creatorcontrib>Ward, J. F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Milligan, J. R.</au><au>Aguilera, J. A.</au><au>Ward, J. F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variation of Single-Strand Break Yield with Scavenger Concentration for Plasmid DNA Irradiated in Aqueous Solution</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>1993-02-01</date><risdate>1993</risdate><volume>133</volume><issue>2</issue><spage>151</spage><epage>157</epage><pages>151-157</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><coden>RAREAE</coden><abstract>We have measured the yield of single-strand breaks (SSBs), induced by 137 Cs γ radiation as assayed by agarose gel electrophoresis, for three plasmids and SV40 DNA irradiated in aerobic aqueous solution. DNA SSBs are caused mainly by the hydroxyl radical under these conditions. To characterize the reactivity of DNA with the hydroxyl radical, we investigated the variation of the G value for SSBs [G(SSB)] with the concentration of added hydroxyl radical scavengers. We find that simple competition kinetics does not describe our results, but that a nonhomogeneous kinetics model is in good agreement. At a DNA concentration of 50 μg cm-3, G(SSB) for the direct effect is about $1\times 10^{-5}\ \mu {\rm mol}\ {\rm J}^{-1}$ for the DNA substrates studied. This is equivalent to 2× 10-10 SSB ${\rm Gy}^{-1}\ {\rm Da}^{-1}$. Estimates of the efficiency of SSB induction per ${\rm OH}^{\bullet}$ radical interaction with DNA (0.32-0.44) reveal that all plasmids are essentially equal in reactivity.</abstract><cop>Oak Brook, Il</cop><pub>Academic Press, Inc</pub><pmid>8382368</pmid><doi>10.2307/3578350</doi><tpages>7</tpages></addata></record> |
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| subjects | Aqueous solutions Biological and medical sciences Biological effects of radiation Cesium Radioisotopes DNA DNA Damage DNA, Single-Stranded - radiation effects DNA, Viral - radiation effects Free Radical Scavengers Fundamental and applied biological sciences. Psychology Gamma Rays Gels Genetic variation Hydroxyl radicals Ionizing radiations Kinetics Plasmids Radiation dosage Radiation Genetics Radius of a sphere Scavenging Simian virus 40 Solutions Tissues, organs and organisms biophysics Water |
| title | Variation of Single-Strand Break Yield with Scavenger Concentration for Plasmid DNA Irradiated in Aqueous Solution |
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