The Rate of Progression of Radiation-Transformed Mammary Epithelial Cells Is Enhanced after Low-Dose-Rate Neutron Irradiation
Studies in this laboratory have shown enhancement of the mammary tumorigenic effects of neutron irradiation after low-dose-rate neutron exposures. To investigate possible reasons, a mammary cell system was used which allows quantitation of initiated mammary epithelial cells and examination of the pr...
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| Veröffentlicht in: | Radiat. Res.; (United States) 1986-01, Vol.105 (1), p.68-75 |
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| description | Studies in this laboratory have shown enhancement of the mammary tumorigenic effects of neutron irradiation after low-dose-rate neutron exposures. To investigate possible reasons, a mammary cell system was used which allows quantitation of initiated mammary epithelial cells and examination of the progression of these radiation-altered cells toward the neoplastic phenotype. Female BALB/c mice were irradiated with fission-spectrum neutrons at dose rates of 1 rad/min or 1 rad/day. Twenty-four hours or 16 weeks after irradiation, mammary cells were obtained by enzymatic dissociation. Mammary outgrowths were derived by injection of 104 cells into gland-free fat pads of 3-week-old female BALB/c mice. The frequency of ductal dysplasias in outgrowths from cells irradiated at high or low dose rates was similar. Persistence of dysplasias differed markedly. Few of the dysplasias in outgrowths derived from cells irradiated at the high dose rate persisted, while a large fraction of the dysplasias in outgrowths derived from cells irradiated at low dose rate persisted. When cells remained in situ for 16 weeks prior to dissociation a higher frequency of persistent altered cells was also observed in outgrowths derived from cells irradiated at low neutron dose rates. These data suggest that low-dose-rate neutron exposures enhance the probability of progression of carcinogen-altered cells rather than increase the numbers of initiated cells. |
| doi_str_mv | 10.2307/3576726 |
| format | Article |
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L.</creator><creatorcontrib>Ullrich, R. L. ; Oak Ridge National Lab., TN</creatorcontrib><description>Studies in this laboratory have shown enhancement of the mammary tumorigenic effects of neutron irradiation after low-dose-rate neutron exposures. To investigate possible reasons, a mammary cell system was used which allows quantitation of initiated mammary epithelial cells and examination of the progression of these radiation-altered cells toward the neoplastic phenotype. Female BALB/c mice were irradiated with fission-spectrum neutrons at dose rates of 1 rad/min or 1 rad/day. Twenty-four hours or 16 weeks after irradiation, mammary cells were obtained by enzymatic dissociation. Mammary outgrowths were derived by injection of 104 cells into gland-free fat pads of 3-week-old female BALB/c mice. The frequency of ductal dysplasias in outgrowths from cells irradiated at high or low dose rates was similar. Persistence of dysplasias differed markedly. Few of the dysplasias in outgrowths derived from cells irradiated at the high dose rate persisted, while a large fraction of the dysplasias in outgrowths derived from cells irradiated at low dose rate persisted. When cells remained in situ for 16 weeks prior to dissociation a higher frequency of persistent altered cells was also observed in outgrowths derived from cells irradiated at low neutron dose rates. These data suggest that low-dose-rate neutron exposures enhance the probability of progression of carcinogen-altered cells rather than increase the numbers of initiated cells.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3576726</identifier><identifier>PMID: 3945726</identifier><identifier>CODEN: RAREAE</identifier><language>eng</language><publisher>Oak Brook, Il: Academic Press, Inc</publisher><subject>560152 - Radiation Effects on Animals- Animals ; ANIMAL TISSUES ; ANIMALS ; BARYONS ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; Biological effects of radiation ; BIOLOGICAL RADIATION EFFECTS ; BODY ; Carcinogens ; Cell Transformation, Neoplastic - pathology ; Cell Transformation, Neoplastic - radiation effects ; CELL TRANSFORMATIONS ; Dosage ; DOSE RATES ; DOSE-RESPONSE RELATIONSHIPS ; ELEMENTARY PARTICLES ; Epithelial cells ; EPITHELIUM ; Female ; FERMIONS ; Fundamental and applied biological sciences. Psychology ; GLANDS ; HADRONS ; Ionizing radiations ; IRRADIATION ; LOW DOSE IRRADIATION ; MAMMALS ; MAMMARY GLANDS ; Mammary Neoplasms, Experimental - etiology ; Mammary Neoplasms, Experimental - pathology ; MICE ; Mice, Inbred BALB C ; Neoplasms, Radiation-Induced - pathology ; Neutron irradiation ; NEUTRONS ; NUCLEONS ; ONCOGENIC TRANSFORMATIONS ; ORGANS ; Phenotypes ; Radiation dosage ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RODENTS ; TISSUES ; Tissues, organs and organisms biophysics ; Tumors ; VERTEBRATES</subject><ispartof>Radiat. 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L.</creatorcontrib><creatorcontrib>Oak Ridge National Lab., TN</creatorcontrib><title>The Rate of Progression of Radiation-Transformed Mammary Epithelial Cells Is Enhanced after Low-Dose-Rate Neutron Irradiation</title><title>Radiat. Res.; (United States)</title><addtitle>Radiat Res</addtitle><description>Studies in this laboratory have shown enhancement of the mammary tumorigenic effects of neutron irradiation after low-dose-rate neutron exposures. To investigate possible reasons, a mammary cell system was used which allows quantitation of initiated mammary epithelial cells and examination of the progression of these radiation-altered cells toward the neoplastic phenotype. Female BALB/c mice were irradiated with fission-spectrum neutrons at dose rates of 1 rad/min or 1 rad/day. Twenty-four hours or 16 weeks after irradiation, mammary cells were obtained by enzymatic dissociation. Mammary outgrowths were derived by injection of 104 cells into gland-free fat pads of 3-week-old female BALB/c mice. The frequency of ductal dysplasias in outgrowths from cells irradiated at high or low dose rates was similar. Persistence of dysplasias differed markedly. Few of the dysplasias in outgrowths derived from cells irradiated at the high dose rate persisted, while a large fraction of the dysplasias in outgrowths derived from cells irradiated at low dose rate persisted. When cells remained in situ for 16 weeks prior to dissociation a higher frequency of persistent altered cells was also observed in outgrowths derived from cells irradiated at low neutron dose rates. These data suggest that low-dose-rate neutron exposures enhance the probability of progression of carcinogen-altered cells rather than increase the numbers of initiated cells.</description><subject>560152 - Radiation Effects on Animals- Animals</subject><subject>ANIMAL TISSUES</subject><subject>ANIMALS</subject><subject>BARYONS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>Biological effects of radiation</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BODY</subject><subject>Carcinogens</subject><subject>Cell Transformation, Neoplastic - pathology</subject><subject>Cell Transformation, Neoplastic - radiation effects</subject><subject>CELL TRANSFORMATIONS</subject><subject>Dosage</subject><subject>DOSE RATES</subject><subject>DOSE-RESPONSE RELATIONSHIPS</subject><subject>ELEMENTARY PARTICLES</subject><subject>Epithelial cells</subject><subject>EPITHELIUM</subject><subject>Female</subject><subject>FERMIONS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLANDS</subject><subject>HADRONS</subject><subject>Ionizing radiations</subject><subject>IRRADIATION</subject><subject>LOW DOSE IRRADIATION</subject><subject>MAMMALS</subject><subject>MAMMARY GLANDS</subject><subject>Mammary Neoplasms, Experimental - etiology</subject><subject>Mammary Neoplasms, Experimental - pathology</subject><subject>MICE</subject><subject>Mice, Inbred BALB C</subject><subject>Neoplasms, Radiation-Induced - pathology</subject><subject>Neutron irradiation</subject><subject>NEUTRONS</subject><subject>NUCLEONS</subject><subject>ONCOGENIC TRANSFORMATIONS</subject><subject>ORGANS</subject><subject>Phenotypes</subject><subject>Radiation dosage</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RODENTS</subject><subject>TISSUES</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Tumors</subject><subject>VERTEBRATES</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LAzEQhoMotVbxFwhBBE-rySab3RylVi3UD0o9L2l2Yle2m5KkiAf_u6ld68nT8DIP7zAPQqeUXKWM5Ncsy0Weij3Up5IVScYJ30d9QhhL8qzID9GR9-8kZipkD_WY5FnE--hrtgA8VQGwNfjF2TcH3te23cSpqmoVYkhmTrXeWLeECj-q5VK5Tzxa1WEBTa0aPISm8Xjs8ahdqFZHSJkADk_sR3JrPSQ_B55gHVxsHjv3W3yMDoxqPJx0c4Be70az4UMyeb4fD28miWaChiSnjOgiMxWjcq5YSgnXc4CMC06EVoIykTPONKlMXkgpKREFUJkCn6eGpxUboPNtr_WhLr2uA-iFtm0LOpQZSwUtRIQut5B21nsHply5evNqSUm5kVx2kiN5tiVX63lUsuM6q3F_0e2V16ox0Z6u_Q4rMiEKIf-wdx-s-_faN5zZjbI</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Ullrich, R. L.</creator><general>Academic Press, Inc</general><general>Radiation Research Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>19860101</creationdate><title>The Rate of Progression of Radiation-Transformed Mammary Epithelial Cells Is Enhanced after Low-Dose-Rate Neutron Irradiation</title><author>Ullrich, R. L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-7130c85fd319ba32104cbee546406ca61367343c0df789991068e192e4b2f42d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>560152 - Radiation Effects on Animals- Animals</topic><topic>ANIMAL TISSUES</topic><topic>ANIMALS</topic><topic>BARYONS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>Biological effects of radiation</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BODY</topic><topic>Carcinogens</topic><topic>Cell Transformation, Neoplastic - pathology</topic><topic>Cell Transformation, Neoplastic - radiation effects</topic><topic>CELL TRANSFORMATIONS</topic><topic>Dosage</topic><topic>DOSE RATES</topic><topic>DOSE-RESPONSE RELATIONSHIPS</topic><topic>ELEMENTARY PARTICLES</topic><topic>Epithelial cells</topic><topic>EPITHELIUM</topic><topic>Female</topic><topic>FERMIONS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLANDS</topic><topic>HADRONS</topic><topic>Ionizing radiations</topic><topic>IRRADIATION</topic><topic>LOW DOSE IRRADIATION</topic><topic>MAMMALS</topic><topic>MAMMARY GLANDS</topic><topic>Mammary Neoplasms, Experimental - etiology</topic><topic>Mammary Neoplasms, Experimental - pathology</topic><topic>MICE</topic><topic>Mice, Inbred BALB C</topic><topic>Neoplasms, Radiation-Induced - pathology</topic><topic>Neutron irradiation</topic><topic>NEUTRONS</topic><topic>NUCLEONS</topic><topic>ONCOGENIC TRANSFORMATIONS</topic><topic>ORGANS</topic><topic>Phenotypes</topic><topic>Radiation dosage</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RODENTS</topic><topic>TISSUES</topic><topic>Tissues, organs and organisms biophysics</topic><topic>Tumors</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ullrich, R. L.</creatorcontrib><creatorcontrib>Oak Ridge National Lab., TN</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Radiat. Res.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ullrich, R. L.</au><aucorp>Oak Ridge National Lab., TN</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Rate of Progression of Radiation-Transformed Mammary Epithelial Cells Is Enhanced after Low-Dose-Rate Neutron Irradiation</atitle><jtitle>Radiat. Res.; (United States)</jtitle><addtitle>Radiat Res</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>105</volume><issue>1</issue><spage>68</spage><epage>75</epage><pages>68-75</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><coden>RAREAE</coden><abstract>Studies in this laboratory have shown enhancement of the mammary tumorigenic effects of neutron irradiation after low-dose-rate neutron exposures. To investigate possible reasons, a mammary cell system was used which allows quantitation of initiated mammary epithelial cells and examination of the progression of these radiation-altered cells toward the neoplastic phenotype. Female BALB/c mice were irradiated with fission-spectrum neutrons at dose rates of 1 rad/min or 1 rad/day. Twenty-four hours or 16 weeks after irradiation, mammary cells were obtained by enzymatic dissociation. Mammary outgrowths were derived by injection of 104 cells into gland-free fat pads of 3-week-old female BALB/c mice. The frequency of ductal dysplasias in outgrowths from cells irradiated at high or low dose rates was similar. Persistence of dysplasias differed markedly. Few of the dysplasias in outgrowths derived from cells irradiated at the high dose rate persisted, while a large fraction of the dysplasias in outgrowths derived from cells irradiated at low dose rate persisted. When cells remained in situ for 16 weeks prior to dissociation a higher frequency of persistent altered cells was also observed in outgrowths derived from cells irradiated at low neutron dose rates. These data suggest that low-dose-rate neutron exposures enhance the probability of progression of carcinogen-altered cells rather than increase the numbers of initiated cells.</abstract><cop>Oak Brook, Il</cop><pub>Academic Press, Inc</pub><pmid>3945726</pmid><doi>10.2307/3576726</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0033-7587 |
| ispartof | Radiat. Res.; (United States), 1986-01, Vol.105 (1), p.68-75 |
| issn | 0033-7587 1938-5404 |
| language | eng |
| recordid | cdi_crossref_primary_10_2307_3576726 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | 560152 - Radiation Effects on Animals- Animals ANIMAL TISSUES ANIMALS BARYONS Biological and medical sciences BIOLOGICAL EFFECTS Biological effects of radiation BIOLOGICAL RADIATION EFFECTS BODY Carcinogens Cell Transformation, Neoplastic - pathology Cell Transformation, Neoplastic - radiation effects CELL TRANSFORMATIONS Dosage DOSE RATES DOSE-RESPONSE RELATIONSHIPS ELEMENTARY PARTICLES Epithelial cells EPITHELIUM Female FERMIONS Fundamental and applied biological sciences. Psychology GLANDS HADRONS Ionizing radiations IRRADIATION LOW DOSE IRRADIATION MAMMALS MAMMARY GLANDS Mammary Neoplasms, Experimental - etiology Mammary Neoplasms, Experimental - pathology MICE Mice, Inbred BALB C Neoplasms, Radiation-Induced - pathology Neutron irradiation NEUTRONS NUCLEONS ONCOGENIC TRANSFORMATIONS ORGANS Phenotypes Radiation dosage RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RODENTS TISSUES Tissues, organs and organisms biophysics Tumors VERTEBRATES |
| title | The Rate of Progression of Radiation-Transformed Mammary Epithelial Cells Is Enhanced after Low-Dose-Rate Neutron Irradiation |
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