An Effect of Elevated Postirradiation pH on the Yield of Double-Strand Breaks in DNA from Irradiated Bacterial Cells

Exposure of DNA isolated from irradiated cells of Escherichia coli to a pH of 9.6 caused a marked increase in the yield of double-strand breaks (dsb). The dsb were measured by sedimentation analysis of E. coli chromosomal DNA using neutral sucrose gradients. After incubation for 4 hr at 37°C and pH...

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Veröffentlicht in:	Radiat. Res.; (United States) 1984-05, Vol.98 (2), p.284-292
Hauptverfasser:	Tilby, Michael J., Loverock, Pamela S., Fielden, E. Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	ACCELERATORS ANOXIA BACTERIA Bacteriology Biological and medical sciences BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS DNA DNA damage DNA, Bacterial - radiation effects Dose-Response Relationship, Radiation DOSE-RESPONSE RELATIONSHIPS Elution ESCHERICHIA COLI Escherichia coli - genetics Escherichia coli - radiation effects Ethanol Fundamental and applied biological sciences. Psychology Genetics Hydrogen-Ion Concentration Irradiation LINEAR ACCELERATORS Microbiology MICROORGANISMS NUCLEIC ACIDS ORGANIC COMPOUNDS Oxygen PH VALUE Radiation damage Radiation dosage RADIATION EFFECTS 560112 > Radiation Effects on Biochemicals-- In Microorganisms-- (-1987) Radiation Genetics RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIOINDUCTION RADIOSENSITIVITY EFFECTS STRAND BREAKS Time Factors
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