DNA Repair Kinetics in Mammalian Cells Following Split-Dose Irradiation
The in situ DNA repair kinetics in intracerebral 9L tumor cells and cerebellar neurons following the second of two 1250- or 2500-rad doses separated by various times have been measured using alkaline sucrose gradients in zonal rotors. For both doses and all times employed, both cell types exhibited...
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| Veröffentlicht in: | Radiat. Res.; (United States) 1984-05, Vol.98 (2), p.242-253 |
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| creator | Wierowski, James V. Thomas, Rolland R. Wheeler, Kenneth T. |
| description | The in situ DNA repair kinetics in intracerebral 9L tumor cells and cerebellar neurons following the second of two 1250- or 2500-rad doses separated by various times have been measured using alkaline sucrose gradients in zonal rotors. For both doses and all times employed, both cell types exhibited biphasic kinetics similar to those observed after single doses. When the two doses were separated by less than 2 hr in neurons (1 hr for tumor cells), the half-time (T1/2) of the slow phase was faster than that expected based on the amount of damage present and remained constant until the observed T1/2 coincided with the expected T1/2. When repair of the damage produced by the first dose was complete, the slow phase after the second dose exhibited the same T1/2 as after a single dose. These results suggest that the accessibility of a fraction of the chromatin is altered for a finite period during the repair process, and upon completion of repair is returned to a state indistinguishable from that existing prior to irradiation. |
| doi_str_mv | 10.2307/3576233 |
| format | Article |
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For both doses and all times employed, both cell types exhibited biphasic kinetics similar to those observed after single doses. When the two doses were separated by less than 2 hr in neurons (1 hr for tumor cells), the half-time (T1/2) of the slow phase was faster than that expected based on the amount of damage present and remained constant until the observed T1/2 coincided with the expected T1/2. When repair of the damage produced by the first dose was complete, the slow phase after the second dose exhibited the same T1/2 as after a single dose. These results suggest that the accessibility of a fraction of the chromatin is altered for a finite period during the repair process, and upon completion of repair is returned to a state indistinguishable from that existing prior to irradiation.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3576233</identifier><identifier>PMID: 6729036</identifier><identifier>CODEN: RAREAE</identifier><language>eng</language><publisher>Oak Brook, Il: Academic Press, Inc</publisher><subject>560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987) ; ALKALI METAL ISOTOPES ; ANIMAL CELLS ; Animals ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; Biological effects of radiation ; BIOLOGICAL RADIATION EFFECTS ; BIOLOGICAL RECOVERY ; BIOLOGICAL REPAIR ; Brain Neoplasms - genetics ; Brain Neoplasms - radiotherapy ; CARBON 14 COMPOUNDS ; Cerebellum - radiation effects ; CESIUM 137 ; CESIUM ISOTOPES ; Cesium Radioisotopes ; CHO cells ; Chromatin ; DATA ; DNA ; DNA damage ; DNA Repair ; DNA, Neoplasm - radiation effects ; ENZYMES ; ESTERASES ; EXPERIMENTAL DATA ; FRACTIONATED IRRADIATION ; Fundamental and applied biological sciences. Psychology ; Gamma Rays ; HYDROLASES ; INFORMATION ; Ionizing radiations ; IRRADIATION ; ISOTOPES ; KINETICS ; LABELLED COMPOUNDS ; Lesions ; Male ; Neoplasm Transplantation ; NERVE CELLS ; Neurons ; NUCLEASES ; NUCLEI ; NUCLEIC ACIDS ; NUMERICAL DATA ; ODD-EVEN NUCLEI ; ORGANIC COMPOUNDS ; PHOSPHODIESTERASES ; Radiation damage ; RADIATION EFFECTS ; Radiation Genetics ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIOISOTOPES ; Rats ; Rats, Inbred F344 ; RECOVERY ; REPAIR ; SOMATIC CELLS ; Time Factors ; Tissues, organs and organisms biophysics ; TUMOR CELLS ; Tumors ; YEARS LIVING RADIOISOTOPES 560121 -- Radiation Effects on Cells-- External Source-- (-1987)</subject><ispartof>Radiat. Res.; (United States), 1984-05, Vol.98 (2), p.242-253</ispartof><rights>Copyright 1984 Academic Press, Inc.</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-b9aaf766b48f7eb6887327294db6bb5c4bfadfd752065cf51ec7866ec05387b23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3576233$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3576233$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8862356$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6729036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6879096$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wierowski, James V.</creatorcontrib><creatorcontrib>Thomas, Rolland R.</creatorcontrib><creatorcontrib>Wheeler, Kenneth T.</creatorcontrib><creatorcontrib>Univ. of Rochester, NY</creatorcontrib><title>DNA Repair Kinetics in Mammalian Cells Following Split-Dose Irradiation</title><title>Radiat. Res.; (United States)</title><addtitle>Radiat Res</addtitle><description>The in situ DNA repair kinetics in intracerebral 9L tumor cells and cerebellar neurons following the second of two 1250- or 2500-rad doses separated by various times have been measured using alkaline sucrose gradients in zonal rotors. For both doses and all times employed, both cell types exhibited biphasic kinetics similar to those observed after single doses. When the two doses were separated by less than 2 hr in neurons (1 hr for tumor cells), the half-time (T1/2) of the slow phase was faster than that expected based on the amount of damage present and remained constant until the observed T1/2 coincided with the expected T1/2. When repair of the damage produced by the first dose was complete, the slow phase after the second dose exhibited the same T1/2 as after a single dose. These results suggest that the accessibility of a fraction of the chromatin is altered for a finite period during the repair process, and upon completion of repair is returned to a state indistinguishable from that existing prior to irradiation.</description><subject>560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)</subject><subject>ALKALI METAL ISOTOPES</subject><subject>ANIMAL CELLS</subject><subject>Animals</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>Biological effects of radiation</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BIOLOGICAL RECOVERY</subject><subject>BIOLOGICAL REPAIR</subject><subject>Brain Neoplasms - genetics</subject><subject>Brain Neoplasms - radiotherapy</subject><subject>CARBON 14 COMPOUNDS</subject><subject>Cerebellum - radiation effects</subject><subject>CESIUM 137</subject><subject>CESIUM ISOTOPES</subject><subject>Cesium Radioisotopes</subject><subject>CHO cells</subject><subject>Chromatin</subject><subject>DATA</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA Repair</subject><subject>DNA, Neoplasm - radiation effects</subject><subject>ENZYMES</subject><subject>ESTERASES</subject><subject>EXPERIMENTAL DATA</subject><subject>FRACTIONATED IRRADIATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma Rays</subject><subject>HYDROLASES</subject><subject>INFORMATION</subject><subject>Ionizing radiations</subject><subject>IRRADIATION</subject><subject>ISOTOPES</subject><subject>KINETICS</subject><subject>LABELLED COMPOUNDS</subject><subject>Lesions</subject><subject>Male</subject><subject>Neoplasm Transplantation</subject><subject>NERVE CELLS</subject><subject>Neurons</subject><subject>NUCLEASES</subject><subject>NUCLEI</subject><subject>NUCLEIC ACIDS</subject><subject>NUMERICAL DATA</subject><subject>ODD-EVEN NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>PHOSPHODIESTERASES</subject><subject>Radiation damage</subject><subject>RADIATION EFFECTS</subject><subject>Radiation Genetics</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIOISOTOPES</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>RECOVERY</subject><subject>REPAIR</subject><subject>SOMATIC CELLS</subject><subject>Time Factors</subject><subject>Tissues, organs and organisms biophysics</subject><subject>TUMOR CELLS</subject><subject>Tumors</subject><subject>YEARS LIVING RADIOISOTOPES 560121 -- Radiation Effects on Cells-- External Source-- (-1987)</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1LwzAUhoMoc07xFwhBBK-qadN89HJsbopTwY_rkqSJZqRNSSLiv7eyMbzx6nB4H95zeAA4zdFVgRG7xoTRAuM9MM4rzDNSonIfjBHCOGOEs0NwFOMaDXtOqxEYUVZUCNMxWM4fp_BZ98IGeG87nayK0HbwQbStcFZ0cKadi3DhnfNftnuHL72zKZv7qOFdCKKxIlnfHYMDI1zUJ9s5AW-Lm9fZbbZ6Wt7NpqtMYZqnTFZCGEapLLlhWlLOGS6GX8pGUimJKqURjWkYKRAlypBcK8Yp1QoRzJks8AScb3p9TLaOyiatPpTvOq1STTmrUEUH6HIDqeBjDNrUfbCtCN91jupfXfVW10Cebcj-U7a62XFbP0N-sc1FVMKZIDpl4w7jfGghf7B1TD78e-0HjgZ62Q</recordid><startdate>198405</startdate><enddate>198405</enddate><creator>Wierowski, James V.</creator><creator>Thomas, Rolland R.</creator><creator>Wheeler, Kenneth T.</creator><general>Academic Press, Inc</general><general>Radiation Research Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>198405</creationdate><title>DNA Repair Kinetics in Mammalian Cells Following Split-Dose Irradiation</title><author>Wierowski, James V. ; Thomas, Rolland R. ; Wheeler, Kenneth T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-b9aaf766b48f7eb6887327294db6bb5c4bfadfd752065cf51ec7866ec05387b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)</topic><topic>ALKALI METAL ISOTOPES</topic><topic>ANIMAL CELLS</topic><topic>Animals</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>Biological effects of radiation</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>Brain Neoplasms - genetics</topic><topic>Brain Neoplasms - radiotherapy</topic><topic>CARBON 14 COMPOUNDS</topic><topic>Cerebellum - radiation effects</topic><topic>CESIUM 137</topic><topic>CESIUM ISOTOPES</topic><topic>Cesium Radioisotopes</topic><topic>CHO cells</topic><topic>Chromatin</topic><topic>DATA</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA Repair</topic><topic>DNA, Neoplasm - radiation effects</topic><topic>ENZYMES</topic><topic>ESTERASES</topic><topic>EXPERIMENTAL DATA</topic><topic>FRACTIONATED IRRADIATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma Rays</topic><topic>HYDROLASES</topic><topic>INFORMATION</topic><topic>Ionizing radiations</topic><topic>IRRADIATION</topic><topic>ISOTOPES</topic><topic>KINETICS</topic><topic>LABELLED COMPOUNDS</topic><topic>Lesions</topic><topic>Male</topic><topic>Neoplasm Transplantation</topic><topic>NERVE CELLS</topic><topic>Neurons</topic><topic>NUCLEASES</topic><topic>NUCLEI</topic><topic>NUCLEIC ACIDS</topic><topic>NUMERICAL DATA</topic><topic>ODD-EVEN NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>PHOSPHODIESTERASES</topic><topic>Radiation damage</topic><topic>RADIATION EFFECTS</topic><topic>Radiation Genetics</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIOISOTOPES</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>RECOVERY</topic><topic>REPAIR</topic><topic>SOMATIC CELLS</topic><topic>Time Factors</topic><topic>Tissues, organs and organisms biophysics</topic><topic>TUMOR CELLS</topic><topic>Tumors</topic><topic>YEARS LIVING RADIOISOTOPES 560121 -- Radiation Effects on Cells-- External Source-- (-1987)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wierowski, James V.</creatorcontrib><creatorcontrib>Thomas, Rolland R.</creatorcontrib><creatorcontrib>Wheeler, Kenneth T.</creatorcontrib><creatorcontrib>Univ. of Rochester, NY</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Radiat. Res.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wierowski, James V.</au><au>Thomas, Rolland R.</au><au>Wheeler, Kenneth T.</au><aucorp>Univ. of Rochester, NY</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Repair Kinetics in Mammalian Cells Following Split-Dose Irradiation</atitle><jtitle>Radiat. Res.; (United States)</jtitle><addtitle>Radiat Res</addtitle><date>1984-05</date><risdate>1984</risdate><volume>98</volume><issue>2</issue><spage>242</spage><epage>253</epage><pages>242-253</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><coden>RAREAE</coden><abstract>The in situ DNA repair kinetics in intracerebral 9L tumor cells and cerebellar neurons following the second of two 1250- or 2500-rad doses separated by various times have been measured using alkaline sucrose gradients in zonal rotors. For both doses and all times employed, both cell types exhibited biphasic kinetics similar to those observed after single doses. When the two doses were separated by less than 2 hr in neurons (1 hr for tumor cells), the half-time (T1/2) of the slow phase was faster than that expected based on the amount of damage present and remained constant until the observed T1/2 coincided with the expected T1/2. When repair of the damage produced by the first dose was complete, the slow phase after the second dose exhibited the same T1/2 as after a single dose. These results suggest that the accessibility of a fraction of the chromatin is altered for a finite period during the repair process, and upon completion of repair is returned to a state indistinguishable from that existing prior to irradiation.</abstract><cop>Oak Brook, Il</cop><pub>Academic Press, Inc</pub><pmid>6729036</pmid><doi>10.2307/3576233</doi><tpages>12</tpages></addata></record> |
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| ispartof | Radiat. Res.; (United States), 1984-05, Vol.98 (2), p.242-253 |
| issn | 0033-7587 1938-5404 |
| language | eng |
| recordid | cdi_crossref_primary_10_2307_3576233 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | 560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987) ALKALI METAL ISOTOPES ANIMAL CELLS Animals BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BIOLOGICAL EFFECTS Biological effects of radiation BIOLOGICAL RADIATION EFFECTS BIOLOGICAL RECOVERY BIOLOGICAL REPAIR Brain Neoplasms - genetics Brain Neoplasms - radiotherapy CARBON 14 COMPOUNDS Cerebellum - radiation effects CESIUM 137 CESIUM ISOTOPES Cesium Radioisotopes CHO cells Chromatin DATA DNA DNA damage DNA Repair DNA, Neoplasm - radiation effects ENZYMES ESTERASES EXPERIMENTAL DATA FRACTIONATED IRRADIATION Fundamental and applied biological sciences. Psychology Gamma Rays HYDROLASES INFORMATION Ionizing radiations IRRADIATION ISOTOPES KINETICS LABELLED COMPOUNDS Lesions Male Neoplasm Transplantation NERVE CELLS Neurons NUCLEASES NUCLEI NUCLEIC ACIDS NUMERICAL DATA ODD-EVEN NUCLEI ORGANIC COMPOUNDS PHOSPHODIESTERASES Radiation damage RADIATION EFFECTS Radiation Genetics RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIOISOTOPES Rats Rats, Inbred F344 RECOVERY REPAIR SOMATIC CELLS Time Factors Tissues, organs and organisms biophysics TUMOR CELLS Tumors YEARS LIVING RADIOISOTOPES 560121 -- Radiation Effects on Cells-- External Source-- (-1987) |
| title | DNA Repair Kinetics in Mammalian Cells Following Split-Dose Irradiation |
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