X-Ray-Induced Structural Changes in Erythrocyte Membranes Studied by Use of Fluorescent Probes
The structural changes in erythrocyte membranes caused by X irradiation were investigated with the aid of fluorescent probes. It was found that the fluorescence intensity of 1-anilino-8-naphthalene sulfonate (ANS) combined with X-irradiated membranes decreased as a function of the dose. The effect c...
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| Veröffentlicht in: | Radiat. Res.; (United States) 1978-07, Vol.75 (1), p.31-45 |
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| creator | Yonei, Shuji Kato, Mikita |
| description | The structural changes in erythrocyte membranes caused by X irradiation were investigated with the aid of fluorescent probes. It was found that the fluorescence intensity of 1-anilino-8-naphthalene sulfonate (ANS) combined with X-irradiated membranes decreased as a function of the dose. The effect could be observed even when 100 R was given. The lowering of the quantum yield of ANS fluorescence in the irradiated membranes seemed to be the primary cause of the fluorescence decrease, as the amount of bound ANS was found to be only slightly decreased. The fluorescence decrease was not accompanied by a significant shift of the emission spectrum to a longer wavelength. In addition, the fluorescence polarization of bound ANS decreased in X-irradiated membranes, suggesting that the decrease in quantum yield of bound ANS might be due to the decrease in membrane microviscosity. Measurement of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene incorporated into the membranes indicated that it also decreased with increasing X-ray doses. The changes in the dynamic properties of erythrocyte membranes caused by X irradiation were further confirmed. From these results it was concluded that X irradiation readily induces significant changes of erythrocyte membrane structure. |
| doi_str_mv | 10.2307/3574866 |
| format | Article |
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It was found that the fluorescence intensity of 1-anilino-8-naphthalene sulfonate (ANS) combined with X-irradiated membranes decreased as a function of the dose. The effect could be observed even when 100 R was given. The lowering of the quantum yield of ANS fluorescence in the irradiated membranes seemed to be the primary cause of the fluorescence decrease, as the amount of bound ANS was found to be only slightly decreased. The fluorescence decrease was not accompanied by a significant shift of the emission spectrum to a longer wavelength. In addition, the fluorescence polarization of bound ANS decreased in X-irradiated membranes, suggesting that the decrease in quantum yield of bound ANS might be due to the decrease in membrane microviscosity. Measurement of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene incorporated into the membranes indicated that it also decreased with increasing X-ray doses. The changes in the dynamic properties of erythrocyte membranes caused by X irradiation were further confirmed. From these results it was concluded that X irradiation readily induces significant changes of erythrocyte membrane structure.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3574866</identifier><identifier>PMID: 684166</identifier><language>eng</language><publisher>United States: Academic Press, Inc</publisher><subject>Aniline Compounds ; BIOLOGICAL EFFECTS ; BIOLOGICAL MATERIALS ; BIOLOGICAL RADIATION EFFECTS ; BLOOD ; BLOOD CELLS ; BODY FLUIDS ; Cell membranes ; Dose-Response Relationship, Radiation ; DOSE-RESPONSE RELATIONSHIPS ; ELECTROMAGNETIC RADIATION ; Erythrocyte membrane ; Erythrocyte Membrane - radiation effects ; Erythrocyte Membrane - ultrastructure ; ERYTHROCYTES ; Erythrocytes - radiation effects ; FLUORESCENCE ; IONIZING RADIATIONS ; Irradiation ; Kinetics ; LUMINESCENCE ; Membrane fluidity ; MEMBRANES ; MORPHOLOGICAL CHANGES ; Naphthalenesulfonates ; ORGANIC COMPOUNDS ; ORGANIC SULFUR COMPOUNDS ; P branes ; Polyenes ; PROBES ; Radiation dosage ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS 560121 -- Radiation Effects on Cells-- External Source-- (-1987) ; RADIOINDUCTION ; Spectrometry, Fluorescence ; String theory ; SULFONATES ; Viscosity ; Wavelengths ; X RADIATION ; X-Rays</subject><ispartof>Radiat. Res.; (United States), 1978-07, Vol.75 (1), p.31-45</ispartof><rights>Copyright 1978 Academic Press, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-f04d7ad991dd3ea2ffd07afab926db885d403f25ea21049824a7a011833e22703</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3574866$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3574866$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/684166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6717133$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Yonei, Shuji</creatorcontrib><creatorcontrib>Kato, Mikita</creatorcontrib><creatorcontrib>Kyoto Univ</creatorcontrib><title>X-Ray-Induced Structural Changes in Erythrocyte Membranes Studied by Use of Fluorescent Probes</title><title>Radiat. Res.; (United States)</title><addtitle>Radiat Res</addtitle><description>The structural changes in erythrocyte membranes caused by X irradiation were investigated with the aid of fluorescent probes. It was found that the fluorescence intensity of 1-anilino-8-naphthalene sulfonate (ANS) combined with X-irradiated membranes decreased as a function of the dose. The effect could be observed even when 100 R was given. The lowering of the quantum yield of ANS fluorescence in the irradiated membranes seemed to be the primary cause of the fluorescence decrease, as the amount of bound ANS was found to be only slightly decreased. The fluorescence decrease was not accompanied by a significant shift of the emission spectrum to a longer wavelength. In addition, the fluorescence polarization of bound ANS decreased in X-irradiated membranes, suggesting that the decrease in quantum yield of bound ANS might be due to the decrease in membrane microviscosity. Measurement of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene incorporated into the membranes indicated that it also decreased with increasing X-ray doses. The changes in the dynamic properties of erythrocyte membranes caused by X irradiation were further confirmed. From these results it was concluded that X irradiation readily induces significant changes of erythrocyte membrane structure.</description><subject>Aniline Compounds</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BLOOD</subject><subject>BLOOD CELLS</subject><subject>BODY FLUIDS</subject><subject>Cell membranes</subject><subject>Dose-Response Relationship, Radiation</subject><subject>DOSE-RESPONSE RELATIONSHIPS</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>Erythrocyte membrane</subject><subject>Erythrocyte Membrane - radiation effects</subject><subject>Erythrocyte Membrane - ultrastructure</subject><subject>ERYTHROCYTES</subject><subject>Erythrocytes - radiation effects</subject><subject>FLUORESCENCE</subject><subject>IONIZING RADIATIONS</subject><subject>Irradiation</subject><subject>Kinetics</subject><subject>LUMINESCENCE</subject><subject>Membrane fluidity</subject><subject>MEMBRANES</subject><subject>MORPHOLOGICAL CHANGES</subject><subject>Naphthalenesulfonates</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC SULFUR COMPOUNDS</subject><subject>P branes</subject><subject>Polyenes</subject><subject>PROBES</subject><subject>Radiation dosage</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS 560121 -- Radiation Effects on Cells-- External Source-- (-1987)</subject><subject>RADIOINDUCTION</subject><subject>Spectrometry, Fluorescence</subject><subject>String theory</subject><subject>SULFONATES</subject><subject>Viscosity</subject><subject>Wavelengths</subject><subject>X RADIATION</subject><subject>X-Rays</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1KAzEYRYP4V6v4Ai6CCK6iySSTZJZSWi1UFGvBlUMmP3aknZQks5i3d2SKO1cfH-fcu7gAXBJ8l1Es7mkumOT8AIxIQSXKGWaHYIQxpUjkUpyCsxi_cf8TXpyAYy4Z4XwEPj_Qm-rQvDGttgYuU2h1aoPawMlaNV82wrqB09CldfC6SxY-220VVNODZWpN3WeqDq6ihd7B2ab1wUZtmwRfg69sPAdHTm2ivdjfMVjNpu-TJ7R4eZxPHhZIU0oScpgZoUxREGOoVZlzBgvlVFVk3FRS5oZh6rK8RwSzQmZMCYUJkZTaLBOYjsH10Otjqsuo62T1WvumsTqVXBBBKO2l20HSwccYrCt3od6q0JUEl78jlvsRe_NqMHdttbXmzxtW6_HNgL9j8uHflh9yOXZr</recordid><startdate>197807</startdate><enddate>197807</enddate><creator>Yonei, Shuji</creator><creator>Kato, Mikita</creator><general>Academic Press, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>197807</creationdate><title>X-Ray-Induced Structural Changes in Erythrocyte Membranes Studied by Use of Fluorescent Probes</title><author>Yonei, Shuji ; Kato, Mikita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-f04d7ad991dd3ea2ffd07afab926db885d403f25ea21049824a7a011833e22703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Aniline Compounds</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BLOOD</topic><topic>BLOOD CELLS</topic><topic>BODY FLUIDS</topic><topic>Cell membranes</topic><topic>Dose-Response Relationship, Radiation</topic><topic>DOSE-RESPONSE RELATIONSHIPS</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>Erythrocyte membrane</topic><topic>Erythrocyte Membrane - radiation effects</topic><topic>Erythrocyte Membrane - ultrastructure</topic><topic>ERYTHROCYTES</topic><topic>Erythrocytes - radiation effects</topic><topic>FLUORESCENCE</topic><topic>IONIZING RADIATIONS</topic><topic>Irradiation</topic><topic>Kinetics</topic><topic>LUMINESCENCE</topic><topic>Membrane fluidity</topic><topic>MEMBRANES</topic><topic>MORPHOLOGICAL CHANGES</topic><topic>Naphthalenesulfonates</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC SULFUR COMPOUNDS</topic><topic>P branes</topic><topic>Polyenes</topic><topic>PROBES</topic><topic>Radiation dosage</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS 560121 -- Radiation Effects on Cells-- External Source-- (-1987)</topic><topic>RADIOINDUCTION</topic><topic>Spectrometry, Fluorescence</topic><topic>String theory</topic><topic>SULFONATES</topic><topic>Viscosity</topic><topic>Wavelengths</topic><topic>X RADIATION</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yonei, Shuji</creatorcontrib><creatorcontrib>Kato, Mikita</creatorcontrib><creatorcontrib>Kyoto Univ</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Radiat. Res.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yonei, Shuji</au><au>Kato, Mikita</au><aucorp>Kyoto Univ</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>X-Ray-Induced Structural Changes in Erythrocyte Membranes Studied by Use of Fluorescent Probes</atitle><jtitle>Radiat. Res.; (United States)</jtitle><addtitle>Radiat Res</addtitle><date>1978-07</date><risdate>1978</risdate><volume>75</volume><issue>1</issue><spage>31</spage><epage>45</epage><pages>31-45</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><abstract>The structural changes in erythrocyte membranes caused by X irradiation were investigated with the aid of fluorescent probes. It was found that the fluorescence intensity of 1-anilino-8-naphthalene sulfonate (ANS) combined with X-irradiated membranes decreased as a function of the dose. The effect could be observed even when 100 R was given. The lowering of the quantum yield of ANS fluorescence in the irradiated membranes seemed to be the primary cause of the fluorescence decrease, as the amount of bound ANS was found to be only slightly decreased. The fluorescence decrease was not accompanied by a significant shift of the emission spectrum to a longer wavelength. In addition, the fluorescence polarization of bound ANS decreased in X-irradiated membranes, suggesting that the decrease in quantum yield of bound ANS might be due to the decrease in membrane microviscosity. Measurement of the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene incorporated into the membranes indicated that it also decreased with increasing X-ray doses. The changes in the dynamic properties of erythrocyte membranes caused by X irradiation were further confirmed. From these results it was concluded that X irradiation readily induces significant changes of erythrocyte membrane structure.</abstract><cop>United States</cop><pub>Academic Press, Inc</pub><pmid>684166</pmid><doi>10.2307/3574866</doi><tpages>15</tpages></addata></record> |
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| ispartof | Radiat. Res.; (United States), 1978-07, Vol.75 (1), p.31-45 |
| issn | 0033-7587 1938-5404 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Aniline Compounds BIOLOGICAL EFFECTS BIOLOGICAL MATERIALS BIOLOGICAL RADIATION EFFECTS BLOOD BLOOD CELLS BODY FLUIDS Cell membranes Dose-Response Relationship, Radiation DOSE-RESPONSE RELATIONSHIPS ELECTROMAGNETIC RADIATION Erythrocyte membrane Erythrocyte Membrane - radiation effects Erythrocyte Membrane - ultrastructure ERYTHROCYTES Erythrocytes - radiation effects FLUORESCENCE IONIZING RADIATIONS Irradiation Kinetics LUMINESCENCE Membrane fluidity MEMBRANES MORPHOLOGICAL CHANGES Naphthalenesulfonates ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS P branes Polyenes PROBES Radiation dosage RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS 560121 -- Radiation Effects on Cells-- External Source-- (-1987) RADIOINDUCTION Spectrometry, Fluorescence String theory SULFONATES Viscosity Wavelengths X RADIATION X-Rays |
| title | X-Ray-Induced Structural Changes in Erythrocyte Membranes Studied by Use of Fluorescent Probes |
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