Electrical Conductivity and Cholinergic Activity of the Rat Gastric Mucosa, At 4 and 170 Hours after 600 R X-Irradiation

Gastric mucosas were prepared for incubation from excised whole stomachs of irradiated and nonirradiated rats. Measurements were made of transmucosal potential, direct current conductivity, and acetylcholine (ACh) response. When suitably prepared, the mucosa showed low conductivity values of $12\ {\...

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Veröffentlicht in:Radiat. Res. 48: No. 1, 116-27(Oct 1971) 116-27(Oct 1971), 1971-10, Vol.48 (1), p.116-127
Hauptverfasser: Vaughan, Burton E., Pessotti, Rita L.
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container_title Radiat. Res. 48: No. 1, 116-27(Oct 1971)
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Pessotti, Rita L.
description Gastric mucosas were prepared for incubation from excised whole stomachs of irradiated and nonirradiated rats. Measurements were made of transmucosal potential, direct current conductivity, and acetylcholine (ACh) response. When suitably prepared, the mucosa showed low conductivity values of $12\ {\rm mmho}/{\rm cm}^{2}$. Conductivity increased markedly after imposing brief electric shock $8\ {\rm mA}/{\rm cm}^{2}$ or after metabolic inhibition (nitrogen). The response, in both cases, was like a depolarization, with the potential decreasing concomitantly. After irradiation, a different type of response occurred after 4 hours and after 170 hours. After 4 hours, the potential was elevated 40% above that of the nonirradiated control, with negligible change in conductivity. No significant change was detected in reactivity toward 10-3 M ACh (added to the baths). After 170 hours, the potential was identical to that of the nonirradiated control, but conductivity showed a consistent increase of about 17%. In these cases, 10-3 M ACh caused a more rapid inhibition of potential. ACh had no effect on conductivity in either radiation group. Analysis of the 4-hour data indicated that the acute effect of radiation was to inhibit cholinergic effector activity without affecting ACh receptor sites. In the 170-hour group, cholinergic effector activity had returned to normal, but irradiation had left the mucosa both more permeable to ions (i.e., increased conductivity) and with increased sensitivity of the ACh receptor sites.
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Pessotti, Rita L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-4de80406fc59c572b70db0f8eff8178cde0f1618293c818398fee2ed800dfc6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>ACETYLCHOLINE</topic><topic>Acetylcholine - pharmacology</topic><topic>ACETYLCHOLINE/radiation effects on response of rat gastric mucosa to, following whole-body exposure, x</topic><topic>Animals</topic><topic>Atropine - pharmacology</topic><topic>Cholinergic receptors</topic><topic>Cholinergics</topic><topic>Conductivity</topic><topic>Depolarization</topic><topic>ELECTRIC CONDUCTIVITY</topic><topic>Electric Conductivity - drug effects</topic><topic>Electric Conductivity - radiation effects</topic><topic>Electric current</topic><topic>Electric Stimulation</topic><topic>Electrodes</topic><topic>Electrophysiology - radiation effects</topic><topic>Energy metabolism</topic><topic>Female</topic><topic>Gastric mucosa</topic><topic>Gastric Mucosa - drug effects</topic><topic>Gastric Mucosa - innervation</topic><topic>Gastric Mucosa - radiation effects</topic><topic>In Vitro Techniques</topic><topic>Irradiation</topic><topic>Membrane Potentials - radiation effects</topic><topic>Methods</topic><topic>MUCOSA</topic><topic>MUCOSA/radiation effects on cholinergic activity and electrical conductivity of rat gastric, following whole-body exposure, x</topic><topic>N28620 -Life Sciences-Radiation Effects on Animals- Vertebrates</topic><topic>Neuroeffector Junction - drug effects</topic><topic>Neuroeffector Junction - radiation effects</topic><topic>RADIATION EFFECTS</topic><topic>RATS</topic><topic>RATS/radiation effects on cholinergic activity and electrical conductivity of gastric mucosa from, following whole-body exposure, x</topic><topic>Receptors, Cholinergic - radiation effects</topic><topic>SENSITIVITY</topic><topic>STOMACH</topic><topic>TIME DEPENDENCE</topic><topic>Time Factors</topic><topic>WHOLE-BODY IRRADIATION</topic><topic>X RADIATION</topic><topic>X RADIATION/effects on cholinergic activity and electrical conductivity of gastric mucosa from rats following whole-body exposure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vaughan, Burton E.</creatorcontrib><creatorcontrib>Pessotti, Rita L.</creatorcontrib><creatorcontrib>Battelle Memorial Inst., Richland, Wash</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Radiat. Res. 48: No. 1, 116-27(Oct 1971)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vaughan, Burton E.</au><au>Pessotti, Rita L.</au><aucorp>Battelle Memorial Inst., Richland, Wash</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrical Conductivity and Cholinergic Activity of the Rat Gastric Mucosa, At 4 and 170 Hours after 600 R X-Irradiation</atitle><jtitle>Radiat. Res. 48: No. 1, 116-27(Oct 1971)</jtitle><addtitle>Radiat Res</addtitle><date>1971-10</date><risdate>1971</risdate><volume>48</volume><issue>1</issue><spage>116</spage><epage>127</epage><pages>116-127</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><abstract>Gastric mucosas were prepared for incubation from excised whole stomachs of irradiated and nonirradiated rats. Measurements were made of transmucosal potential, direct current conductivity, and acetylcholine (ACh) response. When suitably prepared, the mucosa showed low conductivity values of $12\ {\rm mmho}/{\rm cm}^{2}$. Conductivity increased markedly after imposing brief electric shock $8\ {\rm mA}/{\rm cm}^{2}$ or after metabolic inhibition (nitrogen). The response, in both cases, was like a depolarization, with the potential decreasing concomitantly. After irradiation, a different type of response occurred after 4 hours and after 170 hours. After 4 hours, the potential was elevated 40% above that of the nonirradiated control, with negligible change in conductivity. No significant change was detected in reactivity toward 10-3 M ACh (added to the baths). After 170 hours, the potential was identical to that of the nonirradiated control, but conductivity showed a consistent increase of about 17%. In these cases, 10-3 M ACh caused a more rapid inhibition of potential. ACh had no effect on conductivity in either radiation group. Analysis of the 4-hour data indicated that the acute effect of radiation was to inhibit cholinergic effector activity without affecting ACh receptor sites. In the 170-hour group, cholinergic effector activity had returned to normal, but irradiation had left the mucosa both more permeable to ions (i.e., increased conductivity) and with increased sensitivity of the ACh receptor sites.</abstract><cop>United States</cop><pub>Academic Press, Inc</pub><pmid>5117377</pmid><doi>10.2307/3573400</doi><tpages>12</tpages></addata></record>
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identifier ISSN: 0033-7587
ispartof Radiat. Res. 48: No. 1, 116-27(Oct 1971), 1971-10, Vol.48 (1), p.116-127
issn 0033-7587
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subjects ACETYLCHOLINE
Acetylcholine - pharmacology
ACETYLCHOLINE/radiation effects on response of rat gastric mucosa to, following whole-body exposure, x
Animals
Atropine - pharmacology
Cholinergic receptors
Cholinergics
Conductivity
Depolarization
ELECTRIC CONDUCTIVITY
Electric Conductivity - drug effects
Electric Conductivity - radiation effects
Electric current
Electric Stimulation
Electrodes
Electrophysiology - radiation effects
Energy metabolism
Female
Gastric mucosa
Gastric Mucosa - drug effects
Gastric Mucosa - innervation
Gastric Mucosa - radiation effects
In Vitro Techniques
Irradiation
Membrane Potentials - radiation effects
Methods
MUCOSA
MUCOSA/radiation effects on cholinergic activity and electrical conductivity of rat gastric, following whole-body exposure, x
N28620 -Life Sciences-Radiation Effects on Animals- Vertebrates
Neuroeffector Junction - drug effects
Neuroeffector Junction - radiation effects
RADIATION EFFECTS
RATS
RATS/radiation effects on cholinergic activity and electrical conductivity of gastric mucosa from, following whole-body exposure, x
Receptors, Cholinergic - radiation effects
SENSITIVITY
STOMACH
TIME DEPENDENCE
Time Factors
WHOLE-BODY IRRADIATION
X RADIATION
X RADIATION/effects on cholinergic activity and electrical conductivity of gastric mucosa from rats following whole-body exposure
title Electrical Conductivity and Cholinergic Activity of the Rat Gastric Mucosa, At 4 and 170 Hours after 600 R X-Irradiation
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