Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate

Radiopharmaceuticals based on 90Y are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a 90Sr/90Y generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA0,Tyr3] octreotate and the preparation of [90Y-DOTA0,Tyr3] octreotate (90Y-DOTATATE) for peptide receptore radionuclide therapy. 90Sr/90Y generator was based on the electrochemical separation of 90Y from 90Sr in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionuclidic purity of the 90Y solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mg DOTATATE) with 90YCl3 was performed at 95?C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for 90Y. The achieved efficiency of the 90Sr/90Y generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with 90Y exhibited a high efficiency, too: there was less than 1% of 90Y3+in the 90Y-DOTATATE. Radiofarmaceutici bazirani na 90Y se sve vise koriste u tretmanu malignih oboljenja. Da bi se izislo u susret buducim potrebama, razvijen je 90Sr/90Y generator. Dobiveni 90Y eluat je koriscen za radioobelezavanje analoga konjugovanog DOTA somatostatina [DOTA0, Tyr3] octreotate i pripremanje [90Y-DOTA0, Tyr3] octreotate (90Y-DOTATATE), peptidnim receptorima u radionuklidnoj terapiji. 90Sr/90Y generator je baziran na elektrohemijskom odvajanju 90Y od 90Sr u dvostepenoj elektrolizi. Sistem sa tri elektrode je koriscen za obe elektrolize. U svakoj elektrolizi potencijal radne elektrode je odrzavan konstantnim, dok je pH vrednost rastvora bila podesena na 2,7. Radionuklidna cistoca rastvora 90Y je analizirana pomocu instant tankoslojne i papirne hromatografije. Peptid (100 mg DOTATATE) je obelezavan 30 minuta na 95?C. Radiohemijska cistoca je odredjena pomocu HPLC i hromatografskim razdvajanjem koristeci cvrstu SepPak C-18 kolonu. Dobiveni rezultati potvrdjuju efikasnost kako elektrohemijskog odvajan