PCR confirmation of Chlamydophila felis from nasal and conjunctival swab samples of a domestic cat in Croatia
Until now there is no evidence that chlamydiosis does exist among cats on the Republic of Croatia regardless of the fact that feline chlamydiosis is a worldwide disease. This report describes the clinical examination of a one year old cat with bilateral conjunctivitis, as well as the diagnostic meth...
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| Veröffentlicht in: | Acta veterinaria (Beograd) 2010, Vol.60 (1), p.23-29 |
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| creator | Greguric-Gracner, G., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine Vlahovic, K., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine Slavec, B., University of Ljubljana, Ljubljana (Slovenia). Faculty of Veterinary Medicine Gracner, D., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine Dovc, A., University of Ljubljana, Ljubljana (Slovenia). Faculty of Veterinary Medicine |
| description | Until now there is no evidence that chlamydiosis does exist among cats on the Republic of Croatia regardless of the fact that feline chlamydiosis is a worldwide disease. This report describes the clinical examination of a one year old cat with bilateral conjunctivitis, as well as the diagnostic method used to confirm infection with Chlamydophila (Cp.) felis. We have used rapid immunoassay test (EIA) for antigen detection, in order to examine the swabs taken from the eyes and nostrils. The positive results was confirmed by direct immunofluorescence (DIF) made on scrapings from cat conjunctivas. Conventional polymerase chain reaction (PCR) made on swabs taken from cat conjunctivas and nostrils and sequencing the PCR product was used to confirmed infection of cat precisely with Chlamydophila felis. No increase of IgG antibodies against chlamidias was noted using indirect immunofluorescence (IFA) method. |
| doi_str_mv | 10.2298/AVB1001023G |
| format | Article |
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Faculty of Veterinary Medicine ; Vlahovic, K., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine ; Slavec, B., University of Ljubljana, Ljubljana (Slovenia). Faculty of Veterinary Medicine ; Gracner, D., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine ; Dovc, A., University of Ljubljana, Ljubljana (Slovenia). Faculty of Veterinary Medicine</creator><creatorcontrib>Greguric-Gracner, G., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine ; Vlahovic, K., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine ; Slavec, B., University of Ljubljana, Ljubljana (Slovenia). Faculty of Veterinary Medicine ; Gracner, D., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine ; Dovc, A., University of Ljubljana, Ljubljana (Slovenia). 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No increase of IgG antibodies against chlamidias was noted using indirect immunofluorescence (IFA) method.</description><identifier>ISSN: 0567-8315</identifier><identifier>EISSN: 1820-7448</identifier><identifier>DOI: 10.2298/AVB1001023G</identifier><language>eng</language><subject>CATS ; CHAT ; CHLAMYDIA ; CHLAMYDIA PSITTACI ; CROACIA ; CROATIA ; CROATIE ; DIAGNOSTIC TECHNIQUES ; GATO ; http://www.fao.org/aos/agrovoc#c_1390 ; http://www.fao.org/aos/agrovoc#c_1563 ; http://www.fao.org/aos/agrovoc#c_16712 ; http://www.fao.org/aos/agrovoc#c_33969 ; http://www.fao.org/aos/agrovoc#c_34407 ; http://www.fao.org/aos/agrovoc#c_4788 ; METHODE ; METHODS ; METODOS ; TECNICAS DE DIAGNOSIS</subject><ispartof>Acta veterinaria (Beograd), 2010, Vol.60 (1), p.23-29</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c292t-1261aba1a1a513abebdb69c311b45560d143593aed71802abdc1acf5a7395e663</citedby><cites>FETCH-LOGICAL-c292t-1261aba1a1a513abebdb69c311b45560d143593aed71802abdc1acf5a7395e663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Greguric-Gracner, G., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine</creatorcontrib><creatorcontrib>Vlahovic, K., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine</creatorcontrib><creatorcontrib>Slavec, B., University of Ljubljana, Ljubljana (Slovenia). Faculty of Veterinary Medicine</creatorcontrib><creatorcontrib>Gracner, D., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine</creatorcontrib><creatorcontrib>Dovc, A., University of Ljubljana, Ljubljana (Slovenia). Faculty of Veterinary Medicine</creatorcontrib><title>PCR confirmation of Chlamydophila felis from nasal and conjunctival swab samples of a domestic cat in Croatia</title><title>Acta veterinaria (Beograd)</title><description>Until now there is no evidence that chlamydiosis does exist among cats on the Republic of Croatia regardless of the fact that feline chlamydiosis is a worldwide disease. This report describes the clinical examination of a one year old cat with bilateral conjunctivitis, as well as the diagnostic method used to confirm infection with Chlamydophila (Cp.) felis. We have used rapid immunoassay test (EIA) for antigen detection, in order to examine the swabs taken from the eyes and nostrils. The positive results was confirmed by direct immunofluorescence (DIF) made on scrapings from cat conjunctivas. Conventional polymerase chain reaction (PCR) made on swabs taken from cat conjunctivas and nostrils and sequencing the PCR product was used to confirmed infection of cat precisely with Chlamydophila felis. No increase of IgG antibodies against chlamidias was noted using indirect immunofluorescence (IFA) method.</description><subject>CATS</subject><subject>CHAT</subject><subject>CHLAMYDIA</subject><subject>CHLAMYDIA PSITTACI</subject><subject>CROACIA</subject><subject>CROATIA</subject><subject>CROATIE</subject><subject>DIAGNOSTIC TECHNIQUES</subject><subject>GATO</subject><subject>http://www.fao.org/aos/agrovoc#c_1390</subject><subject>http://www.fao.org/aos/agrovoc#c_1563</subject><subject>http://www.fao.org/aos/agrovoc#c_16712</subject><subject>http://www.fao.org/aos/agrovoc#c_33969</subject><subject>http://www.fao.org/aos/agrovoc#c_34407</subject><subject>http://www.fao.org/aos/agrovoc#c_4788</subject><subject>METHODE</subject><subject>METHODS</subject><subject>METODOS</subject><subject>TECNICAS DE DIAGNOSIS</subject><issn>0567-8315</issn><issn>1820-7448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNpNUE1LxDAUDKLgunryLOQu1byk6cdxLboKC8r6cS2v-XCztE1JqrL_3i7rwTeHB-_NDMMQcgnshvOyuF183AFjwLhYHpEZFJwleZoWx2TGZJYnhQB5Ss5i3DImcymzGeleqjVVvrcudDg631NvabVpsdtpP2xci9Sa1kVqg-9ojxFbir3eS7ZfvRrd93SIP9jQiN3QmrjXI9W-M3F0iiocqetpFfzkjufkxGIbzcXfnpP3h_u36jFZPS-fqsUqUbzkYwI8A2wQJkgQ2JhGN1mpBECTTqmZhlTIUqDRORSMY6MVoLISc1FKk2ViTq4Pvir4GIOx9RBch2FXA6v3TdX_mprYVwe2RV_jZ3CxXr_y6TlNIQrxC70BZUI</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Greguric-Gracner, G., University of Zagreb, Zagreb (Croatia). 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Faculty of Veterinary Medicine ; Gracner, D., University of Zagreb, Zagreb (Croatia). Faculty of Veterinary Medicine ; Dovc, A., University of Ljubljana, Ljubljana (Slovenia). 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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | CATS CHAT CHLAMYDIA CHLAMYDIA PSITTACI CROACIA CROATIA CROATIE DIAGNOSTIC TECHNIQUES GATO http://www.fao.org/aos/agrovoc#c_1390 http://www.fao.org/aos/agrovoc#c_1563 http://www.fao.org/aos/agrovoc#c_16712 http://www.fao.org/aos/agrovoc#c_33969 http://www.fao.org/aos/agrovoc#c_34407 http://www.fao.org/aos/agrovoc#c_4788 METHODE METHODS METODOS TECNICAS DE DIAGNOSIS |
| title | PCR confirmation of Chlamydophila felis from nasal and conjunctival swab samples of a domestic cat in Croatia |
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