Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water

The production extended spectrum beta lactamase (ESBL) and metallo-beta lactamase of strains grown biofilm that were isolated from Kilis State Hospital sewage water were investigated in this study. Congo red agar, tube adherence, tissue culture plate tests and SEM analysis were performed for the detection of biofilm production. The extended spectrum beta lactamase and metallo-beta lactamase expression of biofilm producing bacteria was tested by using Kirby-Bauer disc diffusion method according to CLSI guidelines. 5 clinical isolates were revealed to be biofilm producer. From of these isolates, Enterococcus spp. (C2) and E. coli strains showed strong biofilm producing. One of Enterococcus isolates, SEM micrograph of C1 was viewed the weak adherence on glass surface. Only one from 5 isolates, Enterococcus spp. (C2) strain was determined to synthesis ESBL enzyme. None of strains was observed to not produce metallo-beta lactamase. ESBL and biofilm production provide an important pathogenic character allowing protection against antibiotic treatments to Enterococcus spp. (C2).