Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats
To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 ...
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| Veröffentlicht in: | Epigenomics 2021-02, Vol.13 (3), p.187-202 |
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| container_title | Epigenomics |
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| creator | Matboli, Marwa Ibrahim, Doaa Hasanin, Amany H Hassan, Mohamed K Habib, Eman K Bekhet, Miram M Afifi, Ahmed M Eissa, Sanaa |
| description | To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels.
Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (
and
) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting.
Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes.
We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators. |
| doi_str_mv | 10.2217/epi-2020-0353 |
| format | Article |
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Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (
and
) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting.
Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes.
We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.</description><identifier>ISSN: 1750-1911</identifier><identifier>EISSN: 1750-192X</identifier><identifier>DOI: 10.2217/epi-2020-0353</identifier><identifier>PMID: 33406900</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Animals ; autophagy ; Autophagy - drug effects ; Autophagy - genetics ; bioinformatics ; Diabetes Mellitus, Experimental - complications ; Diabetes Mellitus, Type 2 - complications ; Diabetic Nephropathies - drug therapy ; Diabetic Nephropathies - genetics ; Diabetic Nephropathies - metabolism ; Diabetic Nephropathies - pathology ; diabetic nephropathy ; Epigenesis, Genetic - drug effects ; epigenetics ; flavonoid plants ; isorhamnetin ; Kidney - drug effects ; Kidney - metabolism ; Kidney - pathology ; Kidney Tubules - drug effects ; Kidney Tubules - ultrastructure ; Male ; MicroRNAs - metabolism ; miRNA ; Quercetin - analogs & derivatives ; Quercetin - therapeutic use ; Rats ; Rats, Wistar ; RNA ; rtPCR ; western blot</subject><ispartof>Epigenomics, 2021-02, Vol.13 (3), p.187-202</ispartof><rights>2021 Future Medicine Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-335a1e3a1af82870c383283668feb289549e6b14e982fc14516bdcd97335ec973</citedby><cites>FETCH-LOGICAL-c343t-335a1e3a1af82870c383283668feb289549e6b14e982fc14516bdcd97335ec973</cites><orcidid>0000-0001-8591-3244 ; 0000-0002-6852-3954</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33406900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matboli, Marwa</creatorcontrib><creatorcontrib>Ibrahim, Doaa</creatorcontrib><creatorcontrib>Hasanin, Amany H</creatorcontrib><creatorcontrib>Hassan, Mohamed K</creatorcontrib><creatorcontrib>Habib, Eman K</creatorcontrib><creatorcontrib>Bekhet, Miram M</creatorcontrib><creatorcontrib>Afifi, Ahmed M</creatorcontrib><creatorcontrib>Eissa, Sanaa</creatorcontrib><title>Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats</title><title>Epigenomics</title><addtitle>Epigenomics</addtitle><description>To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels.
Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (
and
) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting.
Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes.
We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.</description><subject>Animals</subject><subject>autophagy</subject><subject>Autophagy - drug effects</subject><subject>Autophagy - genetics</subject><subject>bioinformatics</subject><subject>Diabetes Mellitus, Experimental - complications</subject><subject>Diabetes Mellitus, Type 2 - complications</subject><subject>Diabetic Nephropathies - drug therapy</subject><subject>Diabetic Nephropathies - genetics</subject><subject>Diabetic Nephropathies - metabolism</subject><subject>Diabetic Nephropathies - pathology</subject><subject>diabetic nephropathy</subject><subject>Epigenesis, Genetic - drug effects</subject><subject>epigenetics</subject><subject>flavonoid plants</subject><subject>isorhamnetin</subject><subject>Kidney - drug effects</subject><subject>Kidney - metabolism</subject><subject>Kidney - pathology</subject><subject>Kidney Tubules - drug effects</subject><subject>Kidney Tubules - ultrastructure</subject><subject>Male</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Quercetin - analogs & derivatives</subject><subject>Quercetin - therapeutic use</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA</subject><subject>rtPCR</subject><subject>western blot</subject><issn>1750-1911</issn><issn>1750-192X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtqwzAQRUVpaUKaZbdFP-BWDz-XJaQPCHSTQndGlsexWtsSkrzwL_SrKzdpdh0EM2LuPTAXoVtK7hmj2QMYFTHCSER4wi_QkmYJiWjBPi7PM6ULtHbuk4TiLC9Seo0WnMckLQhZou-tUQcYwCuJe12PnfBKD1g3WIxem1YcJjzvHe7U8AU19hrXSlS_hgFMa7URvp1wNWFR92pQztszQzltW9HP-AGHt58MYHYCBGYPXaf86HCwuBt01YjOwfrUV-j9abvfvES7t-fXzeMukjzmPuI8ERS4oKLJWZ4RyfNwF0_TvIEqHJjEBaQVjaHIWSNpnNC0qmVdZMEIMrQVio5cabVzFprSWNULO5WUlHOsZYi1nGMt51iD_u6oN2PVQ31W_4UYBMVR0Ix-tOCkgkFCefwFh5JqgH_gPwohiWM</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Matboli, Marwa</creator><creator>Ibrahim, Doaa</creator><creator>Hasanin, Amany H</creator><creator>Hassan, Mohamed K</creator><creator>Habib, Eman K</creator><creator>Bekhet, Miram M</creator><creator>Afifi, Ahmed M</creator><creator>Eissa, Sanaa</creator><general>Future Medicine Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-8591-3244</orcidid><orcidid>https://orcid.org/0000-0002-6852-3954</orcidid></search><sort><creationdate>20210201</creationdate><title>Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats</title><author>Matboli, Marwa ; Ibrahim, Doaa ; Hasanin, Amany H ; Hassan, Mohamed K ; Habib, Eman K ; Bekhet, Miram M ; Afifi, Ahmed M ; Eissa, Sanaa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-335a1e3a1af82870c383283668feb289549e6b14e982fc14516bdcd97335ec973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>autophagy</topic><topic>Autophagy - drug effects</topic><topic>Autophagy - genetics</topic><topic>bioinformatics</topic><topic>Diabetes Mellitus, Experimental - complications</topic><topic>Diabetes Mellitus, Type 2 - complications</topic><topic>Diabetic Nephropathies - drug therapy</topic><topic>Diabetic Nephropathies - genetics</topic><topic>Diabetic Nephropathies - metabolism</topic><topic>Diabetic Nephropathies - pathology</topic><topic>diabetic nephropathy</topic><topic>Epigenesis, Genetic - drug effects</topic><topic>epigenetics</topic><topic>flavonoid plants</topic><topic>isorhamnetin</topic><topic>Kidney - drug effects</topic><topic>Kidney - metabolism</topic><topic>Kidney - pathology</topic><topic>Kidney Tubules - drug effects</topic><topic>Kidney Tubules - ultrastructure</topic><topic>Male</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Quercetin - analogs & derivatives</topic><topic>Quercetin - therapeutic use</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA</topic><topic>rtPCR</topic><topic>western blot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matboli, Marwa</creatorcontrib><creatorcontrib>Ibrahim, Doaa</creatorcontrib><creatorcontrib>Hasanin, Amany H</creatorcontrib><creatorcontrib>Hassan, Mohamed K</creatorcontrib><creatorcontrib>Habib, Eman K</creatorcontrib><creatorcontrib>Bekhet, Miram M</creatorcontrib><creatorcontrib>Afifi, Ahmed M</creatorcontrib><creatorcontrib>Eissa, Sanaa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Epigenomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matboli, Marwa</au><au>Ibrahim, Doaa</au><au>Hasanin, Amany H</au><au>Hassan, Mohamed K</au><au>Habib, Eman K</au><au>Bekhet, Miram M</au><au>Afifi, Ahmed M</au><au>Eissa, Sanaa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats</atitle><jtitle>Epigenomics</jtitle><addtitle>Epigenomics</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>13</volume><issue>3</issue><spage>187</spage><epage>202</epage><pages>187-202</pages><issn>1750-1911</issn><eissn>1750-192X</eissn><abstract>To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels.
Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (
and
) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting.
Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes.
We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>33406900</pmid><doi>10.2217/epi-2020-0353</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-8591-3244</orcidid><orcidid>https://orcid.org/0000-0002-6852-3954</orcidid></addata></record> |
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| subjects | Animals autophagy Autophagy - drug effects Autophagy - genetics bioinformatics Diabetes Mellitus, Experimental - complications Diabetes Mellitus, Type 2 - complications Diabetic Nephropathies - drug therapy Diabetic Nephropathies - genetics Diabetic Nephropathies - metabolism Diabetic Nephropathies - pathology diabetic nephropathy Epigenesis, Genetic - drug effects epigenetics flavonoid plants isorhamnetin Kidney - drug effects Kidney - metabolism Kidney - pathology Kidney Tubules - drug effects Kidney Tubules - ultrastructure Male MicroRNAs - metabolism miRNA Quercetin - analogs & derivatives Quercetin - therapeutic use Rats Rats, Wistar RNA rtPCR western blot |
| title | Epigenetic modulation of autophagy genes linked to diabetic nephropathy by administration of isorhamnetin in Type 2 diabetes mellitus rats |
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