Sample preparation method for poorly soluble proteins prior to two-dimensional electrophoresis

Protein sample for two-dimensional electrophoresis (2-DE) is conventionally extracted in a buffer containing urea and non-ionic detergent. However, poorly soluble proteins are hardly introduced to the first-dimensional isoelectric focusing (IEF) under the conventional denaturing condition. Moreover,...

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Veröffentlicht in:	SEIBUTSU BUTSURI KAGAKU 2004/06/15, Vol.48(2), pp.71-73
Hauptverfasser:	Kameyama, Kazuhisa, Toda, Toshifusa
Format:	Artikel
Sprache:	eng
Schlagworte:	poorly soluble proteins sample preparation SDS soluble proteins two-dimensional electrophoresis ultra filtration
Online-Zugang:	Volltext
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