Gene-expression profile reveals the genetic and acquired phenotypes of hyperactive mutant SPORTS rat
Spontaneously Running Tokushima Shikoku (SPORTS) rat is a hyperactive rat strain. However, the causative mutation of this phenotype has not yet been identified. To investigate the molecular basis for the unique phenotype of SPORTS rats, we examined gene-expression profiles by microarray analyses. Am...
Gespeichert in:
| Veröffentlicht in: | The Journal of Medical Investigation 2020, Vol.67(1.2), pp.51-61 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 61 |
|---|---|
| container_issue | 1.2 |
| container_start_page | 51 |
| container_title | The Journal of Medical Investigation |
| container_volume | 67 |
| creator | Horiguchi, Taigo Miyatake, Yumiko Miyoshi, Keiko Tanimura, Ayako Hagita, Hiroko Sakaue, Hiroshi Noma, Takafumi |
| description | Spontaneously Running Tokushima Shikoku (SPORTS) rat is a hyperactive rat strain. However, the causative mutation of this phenotype has not yet been identified. To investigate the molecular basis for the unique phenotype of SPORTS rats, we examined gene-expression profiles by microarray analyses. Among adenylate kinase isozymes that maintain the homeostasis of cellular adenine nucleotide composition in the cell, only adenylate kinase 1 is highly up-regulated in both exercised and sedentary SPORTS rats compared with wild-type (WT) rats, 5.5-fold and 3.3-fold, respectively. Further comparative analyses revealed that genes involved in glucose metabolism were up-regulated in skeletal muscle tissue of exercised SPORTS rats compared with sedentary mutants, whereas genes related to extracellular matrix or region were down-regulated compared with WT rats. In brain tissue of sedentary SPORTS rats, genes associated with defense and catecholamine metabolism were highly expressed compared with WT rats. These findings suggest that genetic mutation(s) in SPORTS rat remodels metabolic demands through differentially regulating gene expression regardless of exercise. Therefore, the SPORTS rats are useful animal model not only for further examining the effects of exercise on metabolism but also for deeply studying the molecular basis how mutation affect the psychological motivation with spontaneous voluntary exercise phenotype. J. Med. Invest. 67 : 51-61, February, 2020 |
| doi_str_mv | 10.2152/jmi.67.51 |
| format | Article |
| fullrecord | <record><control><sourceid>jstage_cross</sourceid><recordid>TN_cdi_crossref_primary_10_2152_jmi_67_51</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>article_jmi_67_1_2_67_51_article_char_en</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3641-e082079ec62191c52a1af5be6d5d0e2f9bf413be032788889aa393a335a30ff63</originalsourceid><addsrcrecordid>eNo9kE9PAjEQxRujiYge_Aa9eljsn22XvZgYomhCghE8N0N3Cktgd20LkW9vFWUu85L5zUveI-SWs4HgStyvt_VAFwPFz0iPy7zM9FAX579aZjwX7JJchbBmTEqlVI9UY2www6_OYwh129DOt67eIPW4R9gEGldIl4mJtaXQVBTs5672WNFuhU0bDx0G2jq6SsKDjfUe6XYXoYl09jZ9n8-oh3hNLlzywpu_3Scfz0_z0Us2mY5fR4-TzEqd8wzZULCiRKsFL7lVAjg4tUBdqYqhcOXC5VwukElRDNOUALKUkJKAZM5p2Sd3R1_r2xA8OtP5egv-YDgzP_WYVI_RhVE8sQ9Hdh0iLPFEgk9JN_hPciOOD6eDXYE32MhvDwZwYQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Gene-expression profile reveals the genetic and acquired phenotypes of hyperactive mutant SPORTS rat</title><source>J-STAGE Free</source><source>Freely Accessible Japanese Titles</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Horiguchi, Taigo ; Miyatake, Yumiko ; Miyoshi, Keiko ; Tanimura, Ayako ; Hagita, Hiroko ; Sakaue, Hiroshi ; Noma, Takafumi</creator><creatorcontrib>Horiguchi, Taigo ; Miyatake, Yumiko ; Miyoshi, Keiko ; Tanimura, Ayako ; Hagita, Hiroko ; Sakaue, Hiroshi ; Noma, Takafumi</creatorcontrib><description>Spontaneously Running Tokushima Shikoku (SPORTS) rat is a hyperactive rat strain. However, the causative mutation of this phenotype has not yet been identified. To investigate the molecular basis for the unique phenotype of SPORTS rats, we examined gene-expression profiles by microarray analyses. Among adenylate kinase isozymes that maintain the homeostasis of cellular adenine nucleotide composition in the cell, only adenylate kinase 1 is highly up-regulated in both exercised and sedentary SPORTS rats compared with wild-type (WT) rats, 5.5-fold and 3.3-fold, respectively. Further comparative analyses revealed that genes involved in glucose metabolism were up-regulated in skeletal muscle tissue of exercised SPORTS rats compared with sedentary mutants, whereas genes related to extracellular matrix or region were down-regulated compared with WT rats. In brain tissue of sedentary SPORTS rats, genes associated with defense and catecholamine metabolism were highly expressed compared with WT rats. These findings suggest that genetic mutation(s) in SPORTS rat remodels metabolic demands through differentially regulating gene expression regardless of exercise. Therefore, the SPORTS rats are useful animal model not only for further examining the effects of exercise on metabolism but also for deeply studying the molecular basis how mutation affect the psychological motivation with spontaneous voluntary exercise phenotype. J. Med. Invest. 67 : 51-61, February, 2020</description><identifier>ISSN: 1343-1420</identifier><identifier>EISSN: 1349-6867</identifier><identifier>DOI: 10.2152/jmi.67.51</identifier><language>eng</language><publisher>The University of Tokushima Faculty of Medicine</publisher><subject>adenylate kinase ; energy metabolism ; microarray analysis ; SPORTS rats ; voluntary exercise</subject><ispartof>The Journal of Medical Investigation, 2020, Vol.67(1.2), pp.51-61</ispartof><rights>2020 by The University of Tokushima Faculty of Medicine</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3641-e082079ec62191c52a1af5be6d5d0e2f9bf413be032788889aa393a335a30ff63</citedby><cites>FETCH-LOGICAL-c3641-e082079ec62191c52a1af5be6d5d0e2f9bf413be032788889aa393a335a30ff63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Horiguchi, Taigo</creatorcontrib><creatorcontrib>Miyatake, Yumiko</creatorcontrib><creatorcontrib>Miyoshi, Keiko</creatorcontrib><creatorcontrib>Tanimura, Ayako</creatorcontrib><creatorcontrib>Hagita, Hiroko</creatorcontrib><creatorcontrib>Sakaue, Hiroshi</creatorcontrib><creatorcontrib>Noma, Takafumi</creatorcontrib><title>Gene-expression profile reveals the genetic and acquired phenotypes of hyperactive mutant SPORTS rat</title><title>The Journal of Medical Investigation</title><addtitle>J. Med. Invest.</addtitle><description>Spontaneously Running Tokushima Shikoku (SPORTS) rat is a hyperactive rat strain. However, the causative mutation of this phenotype has not yet been identified. To investigate the molecular basis for the unique phenotype of SPORTS rats, we examined gene-expression profiles by microarray analyses. Among adenylate kinase isozymes that maintain the homeostasis of cellular adenine nucleotide composition in the cell, only adenylate kinase 1 is highly up-regulated in both exercised and sedentary SPORTS rats compared with wild-type (WT) rats, 5.5-fold and 3.3-fold, respectively. Further comparative analyses revealed that genes involved in glucose metabolism were up-regulated in skeletal muscle tissue of exercised SPORTS rats compared with sedentary mutants, whereas genes related to extracellular matrix or region were down-regulated compared with WT rats. In brain tissue of sedentary SPORTS rats, genes associated with defense and catecholamine metabolism were highly expressed compared with WT rats. These findings suggest that genetic mutation(s) in SPORTS rat remodels metabolic demands through differentially regulating gene expression regardless of exercise. Therefore, the SPORTS rats are useful animal model not only for further examining the effects of exercise on metabolism but also for deeply studying the molecular basis how mutation affect the psychological motivation with spontaneous voluntary exercise phenotype. J. Med. Invest. 67 : 51-61, February, 2020</description><subject>adenylate kinase</subject><subject>energy metabolism</subject><subject>microarray analysis</subject><subject>SPORTS rats</subject><subject>voluntary exercise</subject><issn>1343-1420</issn><issn>1349-6867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNo9kE9PAjEQxRujiYge_Aa9eljsn22XvZgYomhCghE8N0N3Cktgd20LkW9vFWUu85L5zUveI-SWs4HgStyvt_VAFwPFz0iPy7zM9FAX579aZjwX7JJchbBmTEqlVI9UY2www6_OYwh129DOt67eIPW4R9gEGldIl4mJtaXQVBTs5672WNFuhU0bDx0G2jq6SsKDjfUe6XYXoYl09jZ9n8-oh3hNLlzywpu_3Scfz0_z0Us2mY5fR4-TzEqd8wzZULCiRKsFL7lVAjg4tUBdqYqhcOXC5VwukElRDNOUALKUkJKAZM5p2Sd3R1_r2xA8OtP5egv-YDgzP_WYVI_RhVE8sQ9Hdh0iLPFEgk9JN_hPciOOD6eDXYE32MhvDwZwYQ</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Horiguchi, Taigo</creator><creator>Miyatake, Yumiko</creator><creator>Miyoshi, Keiko</creator><creator>Tanimura, Ayako</creator><creator>Hagita, Hiroko</creator><creator>Sakaue, Hiroshi</creator><creator>Noma, Takafumi</creator><general>The University of Tokushima Faculty of Medicine</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2020</creationdate><title>Gene-expression profile reveals the genetic and acquired phenotypes of hyperactive mutant SPORTS rat</title><author>Horiguchi, Taigo ; Miyatake, Yumiko ; Miyoshi, Keiko ; Tanimura, Ayako ; Hagita, Hiroko ; Sakaue, Hiroshi ; Noma, Takafumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3641-e082079ec62191c52a1af5be6d5d0e2f9bf413be032788889aa393a335a30ff63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>adenylate kinase</topic><topic>energy metabolism</topic><topic>microarray analysis</topic><topic>SPORTS rats</topic><topic>voluntary exercise</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horiguchi, Taigo</creatorcontrib><creatorcontrib>Miyatake, Yumiko</creatorcontrib><creatorcontrib>Miyoshi, Keiko</creatorcontrib><creatorcontrib>Tanimura, Ayako</creatorcontrib><creatorcontrib>Hagita, Hiroko</creatorcontrib><creatorcontrib>Sakaue, Hiroshi</creatorcontrib><creatorcontrib>Noma, Takafumi</creatorcontrib><collection>CrossRef</collection><jtitle>The Journal of Medical Investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horiguchi, Taigo</au><au>Miyatake, Yumiko</au><au>Miyoshi, Keiko</au><au>Tanimura, Ayako</au><au>Hagita, Hiroko</au><au>Sakaue, Hiroshi</au><au>Noma, Takafumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene-expression profile reveals the genetic and acquired phenotypes of hyperactive mutant SPORTS rat</atitle><jtitle>The Journal of Medical Investigation</jtitle><addtitle>J. Med. Invest.</addtitle><date>2020</date><risdate>2020</risdate><volume>67</volume><issue>1.2</issue><spage>51</spage><epage>61</epage><pages>51-61</pages><issn>1343-1420</issn><eissn>1349-6867</eissn><abstract>Spontaneously Running Tokushima Shikoku (SPORTS) rat is a hyperactive rat strain. However, the causative mutation of this phenotype has not yet been identified. To investigate the molecular basis for the unique phenotype of SPORTS rats, we examined gene-expression profiles by microarray analyses. Among adenylate kinase isozymes that maintain the homeostasis of cellular adenine nucleotide composition in the cell, only adenylate kinase 1 is highly up-regulated in both exercised and sedentary SPORTS rats compared with wild-type (WT) rats, 5.5-fold and 3.3-fold, respectively. Further comparative analyses revealed that genes involved in glucose metabolism were up-regulated in skeletal muscle tissue of exercised SPORTS rats compared with sedentary mutants, whereas genes related to extracellular matrix or region were down-regulated compared with WT rats. In brain tissue of sedentary SPORTS rats, genes associated with defense and catecholamine metabolism were highly expressed compared with WT rats. These findings suggest that genetic mutation(s) in SPORTS rat remodels metabolic demands through differentially regulating gene expression regardless of exercise. Therefore, the SPORTS rats are useful animal model not only for further examining the effects of exercise on metabolism but also for deeply studying the molecular basis how mutation affect the psychological motivation with spontaneous voluntary exercise phenotype. J. Med. Invest. 67 : 51-61, February, 2020</abstract><pub>The University of Tokushima Faculty of Medicine</pub><doi>10.2152/jmi.67.51</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1343-1420 |
| ispartof | The Journal of Medical Investigation, 2020, Vol.67(1.2), pp.51-61 |
| issn | 1343-1420 1349-6867 |
| language | eng |
| recordid | cdi_crossref_primary_10_2152_jmi_67_51 |
| source | J-STAGE Free; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals |
| subjects | adenylate kinase energy metabolism microarray analysis SPORTS rats voluntary exercise |
| title | Gene-expression profile reveals the genetic and acquired phenotypes of hyperactive mutant SPORTS rat |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T03%3A29%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstage_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gene-expression%20profile%20reveals%20the%20genetic%20and%20acquired%20phenotypes%20of%20hyperactive%20mutant%20SPORTS%20rat&rft.jtitle=The%20Journal%20of%20Medical%20Investigation&rft.au=Horiguchi,%20Taigo&rft.date=2020&rft.volume=67&rft.issue=1.2&rft.spage=51&rft.epage=61&rft.pages=51-61&rft.issn=1343-1420&rft.eissn=1349-6867&rft_id=info:doi/10.2152/jmi.67.51&rft_dat=%3Cjstage_cross%3Earticle_jmi_67_1_2_67_51_article_char_en%3C/jstage_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |