Mineralocorticoid receptor expression in human penile corpus cavernosum
Objectives: Mineralocorticoid receptor (MR) is known to play physiological and pathophysiological roles in the cardiovascular system, and MR activation directly damages these organs. The aim of this study was to evaluate the expression of MR and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) in...
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| Veröffentlicht in: | The Journal of Medical Investigation 2013, Vol.60(1.2), pp.21-26 |
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| container_title | The Journal of Medical Investigation |
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| creator | Kishimoto, Tomoteru Fukawa, Tomoya Yamaguchi, Kunihisa Yamamoto, Yasuyo Nakatsuji, Hiroyoshi Izaki, Hirofumi Takahashi, Masayuki Fukumori, Tomoharu Kanayama, Hiro-omi |
| description | Objectives: Mineralocorticoid receptor (MR) is known to play physiological and pathophysiological roles in the cardiovascular system, and MR activation directly damages these organs. The aim of this study was to evaluate the expression of MR and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) in the human penile corpus cavernosum. Methods: MR and 11β-HSD2 expression was assayed in human penile tissues, and also in human renal tissues as a positive control. Expressions of MR mRNA and 11β-HSD2 mRNA were evaluated using reverse transcription polymerase chain reaction (RT-PCR). MR and 11β-HSD2 were visually identified using immunofluorescence analysis. Results: MR mRNA expression in human penis was confirmed by RT-PCR. On quantitative RT-PCR analysis, 11β-HSD2 mRNA expression was detected at minimal levels in penile tissue. Immunofluorescence analysis revealed positive staining for MR and negative staining for 11β-HSD2 in smooth muscle cells of the corpus cavernosum. Conclusions: This study demonstrated the presence of MR and the absence of 11β-HSD2 in human penile corpus cavernosum. Considering that MR activation causes various organ damages, MR blockade in human penile corpus cavernosum may have therapeutic benefits. Investigations for the penile effects of MR activation have the potential to provide new treatment approaches for erectile dysfunction. J. Med. Invest. 60: 21-26, February, 2013 |
| doi_str_mv | 10.2152/jmi.60.21 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_2152_jmi_60_21</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>23614907</sourcerecordid><originalsourceid>FETCH-LOGICAL-c577t-b4f310dc555e35821ffc586a53ff494469e607efb688ddca289674bb0a2db3f93</originalsourceid><addsrcrecordid>eNo9kE1Lw0AQhhdRbK0e_AOSq4fU_U5yEaRoFSpe9Bw2m1m7IdmE3UT035sY29O8MA8vMw9C1wSvKRH0rmrsWk75BC0J41ksU5mc_mUWE07xAl2EUGHMmBDiHC0ok4RnOFmi7at14FXd6tb3Vre2jDxo6PrWR_DdeQjBti6yLtoPjXJRB87WEI10N4RIqy_wrg1Dc4nOjKoDXP3PFfp4enzfPMe7t-3L5mEXa5EkfVxwwwgu9XgGMJFSYowWqVSCGcMzzmUGEidgCpmmZakVTTOZ8KLAipYFMxlbodu5V_s2BA8m77xtlP_JCc4nGfkoI5dTHtmbme2GooHySB6-H4H7GahCrz7hCKhJRQ2HKpLTufG40Hvlc3DsF3gBcqk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Mineralocorticoid receptor expression in human penile corpus cavernosum</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Open Access Titles of Japan</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Kishimoto, Tomoteru ; Fukawa, Tomoya ; Yamaguchi, Kunihisa ; Yamamoto, Yasuyo ; Nakatsuji, Hiroyoshi ; Izaki, Hirofumi ; Takahashi, Masayuki ; Fukumori, Tomoharu ; Kanayama, Hiro-omi</creator><creatorcontrib>Kishimoto, Tomoteru ; Fukawa, Tomoya ; Yamaguchi, Kunihisa ; Yamamoto, Yasuyo ; Nakatsuji, Hiroyoshi ; Izaki, Hirofumi ; Takahashi, Masayuki ; Fukumori, Tomoharu ; Kanayama, Hiro-omi</creatorcontrib><description>Objectives: Mineralocorticoid receptor (MR) is known to play physiological and pathophysiological roles in the cardiovascular system, and MR activation directly damages these organs. The aim of this study was to evaluate the expression of MR and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) in the human penile corpus cavernosum. Methods: MR and 11β-HSD2 expression was assayed in human penile tissues, and also in human renal tissues as a positive control. Expressions of MR mRNA and 11β-HSD2 mRNA were evaluated using reverse transcription polymerase chain reaction (RT-PCR). MR and 11β-HSD2 were visually identified using immunofluorescence analysis. Results: MR mRNA expression in human penis was confirmed by RT-PCR. On quantitative RT-PCR analysis, 11β-HSD2 mRNA expression was detected at minimal levels in penile tissue. Immunofluorescence analysis revealed positive staining for MR and negative staining for 11β-HSD2 in smooth muscle cells of the corpus cavernosum. Conclusions: This study demonstrated the presence of MR and the absence of 11β-HSD2 in human penile corpus cavernosum. Considering that MR activation causes various organ damages, MR blockade in human penile corpus cavernosum may have therapeutic benefits. Investigations for the penile effects of MR activation have the potential to provide new treatment approaches for erectile dysfunction. J. Med. Invest. 60: 21-26, February, 2013</description><identifier>ISSN: 1343-1420</identifier><identifier>EISSN: 1349-6867</identifier><identifier>DOI: 10.2152/jmi.60.21</identifier><identifier>PMID: 23614907</identifier><language>eng</language><publisher>Japan: The University of Tokushima Faculty of Medicine</publisher><subject>11-beta-hydroxysteroid dehydrogenase type 2 ; 11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics ; Adult ; Aged ; aldosterone ; erectile dysfunction ; Erectile Dysfunction - drug therapy ; Fluorescent Antibody Technique ; Humans ; Male ; Middle Aged ; mineralocorticoid receptor ; Mineralocorticoid Receptor Antagonists - therapeutic use ; penis ; Penis - blood supply ; Penis - chemistry ; Receptors, Mineralocorticoid - analysis ; Receptors, Mineralocorticoid - genetics ; Receptors, Mineralocorticoid - physiology ; RNA, Messenger - analysis</subject><ispartof>The Journal of Medical Investigation, 2013, Vol.60(1.2), pp.21-26</ispartof><rights>2013 by The University of Tokushima Faculty of Medicine</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-b4f310dc555e35821ffc586a53ff494469e607efb688ddca289674bb0a2db3f93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23614907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kishimoto, Tomoteru</creatorcontrib><creatorcontrib>Fukawa, Tomoya</creatorcontrib><creatorcontrib>Yamaguchi, Kunihisa</creatorcontrib><creatorcontrib>Yamamoto, Yasuyo</creatorcontrib><creatorcontrib>Nakatsuji, Hiroyoshi</creatorcontrib><creatorcontrib>Izaki, Hirofumi</creatorcontrib><creatorcontrib>Takahashi, Masayuki</creatorcontrib><creatorcontrib>Fukumori, Tomoharu</creatorcontrib><creatorcontrib>Kanayama, Hiro-omi</creatorcontrib><title>Mineralocorticoid receptor expression in human penile corpus cavernosum</title><title>The Journal of Medical Investigation</title><addtitle>J. Med. Invest.</addtitle><description>Objectives: Mineralocorticoid receptor (MR) is known to play physiological and pathophysiological roles in the cardiovascular system, and MR activation directly damages these organs. The aim of this study was to evaluate the expression of MR and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) in the human penile corpus cavernosum. Methods: MR and 11β-HSD2 expression was assayed in human penile tissues, and also in human renal tissues as a positive control. Expressions of MR mRNA and 11β-HSD2 mRNA were evaluated using reverse transcription polymerase chain reaction (RT-PCR). MR and 11β-HSD2 were visually identified using immunofluorescence analysis. Results: MR mRNA expression in human penis was confirmed by RT-PCR. On quantitative RT-PCR analysis, 11β-HSD2 mRNA expression was detected at minimal levels in penile tissue. Immunofluorescence analysis revealed positive staining for MR and negative staining for 11β-HSD2 in smooth muscle cells of the corpus cavernosum. Conclusions: This study demonstrated the presence of MR and the absence of 11β-HSD2 in human penile corpus cavernosum. Considering that MR activation causes various organ damages, MR blockade in human penile corpus cavernosum may have therapeutic benefits. Investigations for the penile effects of MR activation have the potential to provide new treatment approaches for erectile dysfunction. J. Med. Invest. 60: 21-26, February, 2013</description><subject>11-beta-hydroxysteroid dehydrogenase type 2</subject><subject>11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics</subject><subject>Adult</subject><subject>Aged</subject><subject>aldosterone</subject><subject>erectile dysfunction</subject><subject>Erectile Dysfunction - drug therapy</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>mineralocorticoid receptor</subject><subject>Mineralocorticoid Receptor Antagonists - therapeutic use</subject><subject>penis</subject><subject>Penis - blood supply</subject><subject>Penis - chemistry</subject><subject>Receptors, Mineralocorticoid - analysis</subject><subject>Receptors, Mineralocorticoid - genetics</subject><subject>Receptors, Mineralocorticoid - physiology</subject><subject>RNA, Messenger - analysis</subject><issn>1343-1420</issn><issn>1349-6867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1Lw0AQhhdRbK0e_AOSq4fU_U5yEaRoFSpe9Bw2m1m7IdmE3UT035sY29O8MA8vMw9C1wSvKRH0rmrsWk75BC0J41ksU5mc_mUWE07xAl2EUGHMmBDiHC0ok4RnOFmi7at14FXd6tb3Vre2jDxo6PrWR_DdeQjBti6yLtoPjXJRB87WEI10N4RIqy_wrg1Dc4nOjKoDXP3PFfp4enzfPMe7t-3L5mEXa5EkfVxwwwgu9XgGMJFSYowWqVSCGcMzzmUGEidgCpmmZakVTTOZ8KLAipYFMxlbodu5V_s2BA8m77xtlP_JCc4nGfkoI5dTHtmbme2GooHySB6-H4H7GahCrz7hCKhJRQ2HKpLTufG40Hvlc3DsF3gBcqk</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>Kishimoto, Tomoteru</creator><creator>Fukawa, Tomoya</creator><creator>Yamaguchi, Kunihisa</creator><creator>Yamamoto, Yasuyo</creator><creator>Nakatsuji, Hiroyoshi</creator><creator>Izaki, Hirofumi</creator><creator>Takahashi, Masayuki</creator><creator>Fukumori, Tomoharu</creator><creator>Kanayama, Hiro-omi</creator><general>The University of Tokushima Faculty of Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2013</creationdate><title>Mineralocorticoid receptor expression in human penile corpus cavernosum</title><author>Kishimoto, Tomoteru ; Fukawa, Tomoya ; Yamaguchi, Kunihisa ; Yamamoto, Yasuyo ; Nakatsuji, Hiroyoshi ; Izaki, Hirofumi ; Takahashi, Masayuki ; Fukumori, Tomoharu ; Kanayama, Hiro-omi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-b4f310dc555e35821ffc586a53ff494469e607efb688ddca289674bb0a2db3f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>11-beta-hydroxysteroid dehydrogenase type 2</topic><topic>11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics</topic><topic>Adult</topic><topic>Aged</topic><topic>aldosterone</topic><topic>erectile dysfunction</topic><topic>Erectile Dysfunction - drug therapy</topic><topic>Fluorescent Antibody Technique</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>mineralocorticoid receptor</topic><topic>Mineralocorticoid Receptor Antagonists - therapeutic use</topic><topic>penis</topic><topic>Penis - blood supply</topic><topic>Penis - chemistry</topic><topic>Receptors, Mineralocorticoid - analysis</topic><topic>Receptors, Mineralocorticoid - genetics</topic><topic>Receptors, Mineralocorticoid - physiology</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kishimoto, Tomoteru</creatorcontrib><creatorcontrib>Fukawa, Tomoya</creatorcontrib><creatorcontrib>Yamaguchi, Kunihisa</creatorcontrib><creatorcontrib>Yamamoto, Yasuyo</creatorcontrib><creatorcontrib>Nakatsuji, Hiroyoshi</creatorcontrib><creatorcontrib>Izaki, Hirofumi</creatorcontrib><creatorcontrib>Takahashi, Masayuki</creatorcontrib><creatorcontrib>Fukumori, Tomoharu</creatorcontrib><creatorcontrib>Kanayama, Hiro-omi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The Journal of Medical Investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kishimoto, Tomoteru</au><au>Fukawa, Tomoya</au><au>Yamaguchi, Kunihisa</au><au>Yamamoto, Yasuyo</au><au>Nakatsuji, Hiroyoshi</au><au>Izaki, Hirofumi</au><au>Takahashi, Masayuki</au><au>Fukumori, Tomoharu</au><au>Kanayama, Hiro-omi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mineralocorticoid receptor expression in human penile corpus cavernosum</atitle><jtitle>The Journal of Medical Investigation</jtitle><addtitle>J. Med. Invest.</addtitle><date>2013</date><risdate>2013</risdate><volume>60</volume><issue>1.2</issue><spage>21</spage><epage>26</epage><pages>21-26</pages><issn>1343-1420</issn><eissn>1349-6867</eissn><abstract>Objectives: Mineralocorticoid receptor (MR) is known to play physiological and pathophysiological roles in the cardiovascular system, and MR activation directly damages these organs. The aim of this study was to evaluate the expression of MR and 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) in the human penile corpus cavernosum. Methods: MR and 11β-HSD2 expression was assayed in human penile tissues, and also in human renal tissues as a positive control. Expressions of MR mRNA and 11β-HSD2 mRNA were evaluated using reverse transcription polymerase chain reaction (RT-PCR). MR and 11β-HSD2 were visually identified using immunofluorescence analysis. Results: MR mRNA expression in human penis was confirmed by RT-PCR. On quantitative RT-PCR analysis, 11β-HSD2 mRNA expression was detected at minimal levels in penile tissue. Immunofluorescence analysis revealed positive staining for MR and negative staining for 11β-HSD2 in smooth muscle cells of the corpus cavernosum. Conclusions: This study demonstrated the presence of MR and the absence of 11β-HSD2 in human penile corpus cavernosum. Considering that MR activation causes various organ damages, MR blockade in human penile corpus cavernosum may have therapeutic benefits. Investigations for the penile effects of MR activation have the potential to provide new treatment approaches for erectile dysfunction. J. Med. Invest. 60: 21-26, February, 2013</abstract><cop>Japan</cop><pub>The University of Tokushima Faculty of Medicine</pub><pmid>23614907</pmid><doi>10.2152/jmi.60.21</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 11-beta-hydroxysteroid dehydrogenase type 2 11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics Adult Aged aldosterone erectile dysfunction Erectile Dysfunction - drug therapy Fluorescent Antibody Technique Humans Male Middle Aged mineralocorticoid receptor Mineralocorticoid Receptor Antagonists - therapeutic use penis Penis - blood supply Penis - chemistry Receptors, Mineralocorticoid - analysis Receptors, Mineralocorticoid - genetics Receptors, Mineralocorticoid - physiology RNA, Messenger - analysis |
| title | Mineralocorticoid receptor expression in human penile corpus cavernosum |
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