P 2 Y 2 Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats
P Y receptors (P Y Rs) are among the various receptors that play an important role in nociception. The goal of this research was to investigate possible P Y R expression changes in the trigeminal ganglion (TRG) in bilateral masseter muscle (MM) hypersensitivity following unilateral MM inflammation....
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Veröffentlicht in: | Journal of pain research 2020-06, Vol.13, p.1323-1333 |
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description | P
Y
receptors (P
Y
Rs) are among the various receptors that play an important role in nociception. The goal of this research was to investigate possible P
Y
R expression changes in the trigeminal ganglion (TRG) in bilateral masseter muscle (MM) hypersensitivity following unilateral MM inflammation. The impact of unilateral intramasseteric administration of P
Y
R antagonist on bilateral MM hypersensitivity was also explored.
Bilateral MM hypersensitivity was provoked by unilateral intramasseteric injection of complete Freund's adjuvant (CFA). The head withdrawal threshold (HWT) was assessed bilaterally 4 days later. Bilateral TRG and MM isolation were followed, and quantitative real-time polymerase chain reaction (qRT-PCR) and histopathological analysis were carried out on these tissues, respectively. The involvement of P
Y
Rs in nocifensive behavior was evaluated by administering two doses of P
Y
R antagonist AR-C118925 (0.2 or 1 mg/100 μL) in inflamed MM 4 days post-CFA administration. Bilateral HWT was assessed at different time points following antagonist injection.
qRT-PCR analysis demonstrated P
Y
R up-regulation in TRG ipsilateral to the site of CFA administration. Compared to the controls, both doses of AR-C118925 injected ipsilateral to the TRG increased the bilateral HWT at 30, 60, 90, and 120 minutes after antagonist administration.
The findings suggest that P
Y
Rs may affect MM inflammatory hypersensitivity owing to its up-regulation in the TRG in MM inflammatory pain states. |
doi_str_mv | 10.2147/JPR.S239831 |
format | Article |
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Y
receptors (P
Y
Rs) are among the various receptors that play an important role in nociception. The goal of this research was to investigate possible P
Y
R expression changes in the trigeminal ganglion (TRG) in bilateral masseter muscle (MM) hypersensitivity following unilateral MM inflammation. The impact of unilateral intramasseteric administration of P
Y
R antagonist on bilateral MM hypersensitivity was also explored.
Bilateral MM hypersensitivity was provoked by unilateral intramasseteric injection of complete Freund's adjuvant (CFA). The head withdrawal threshold (HWT) was assessed bilaterally 4 days later. Bilateral TRG and MM isolation were followed, and quantitative real-time polymerase chain reaction (qRT-PCR) and histopathological analysis were carried out on these tissues, respectively. The involvement of P
Y
Rs in nocifensive behavior was evaluated by administering two doses of P
Y
R antagonist AR-C118925 (0.2 or 1 mg/100 μL) in inflamed MM 4 days post-CFA administration. Bilateral HWT was assessed at different time points following antagonist injection.
qRT-PCR analysis demonstrated P
Y
R up-regulation in TRG ipsilateral to the site of CFA administration. Compared to the controls, both doses of AR-C118925 injected ipsilateral to the TRG increased the bilateral HWT at 30, 60, 90, and 120 minutes after antagonist administration.
The findings suggest that P
Y
Rs may affect MM inflammatory hypersensitivity owing to its up-regulation in the TRG in MM inflammatory pain states.</description><identifier>ISSN: 1178-7090</identifier><identifier>EISSN: 1178-7090</identifier><identifier>DOI: 10.2147/JPR.S239831</identifier><identifier>PMID: 32581574</identifier><language>eng</language><publisher>New Zealand</publisher><ispartof>Journal of pain research, 2020-06, Vol.13, p.1323-1333</ispartof><rights>2020 Tariba Knežević et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1381-4bac3ca82be62986818c575d57417824f863a38f40964b64f3bef5065a118f193</citedby><cites>FETCH-LOGICAL-c1381-4bac3ca82be62986818c575d57417824f863a38f40964b64f3bef5065a118f193</cites><orcidid>0000-0001-6333-8533 ; 0000-0002-8323-6515 ; 0000-0002-0671-9820 ; 0000-0002-1360-3279 ; 0000-0002-8117-9203 ; 0000-0002-3961-1728</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,3849,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32581574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tariba Knežević, Petra</creatorcontrib><creatorcontrib>Vukman, Robert</creatorcontrib><creatorcontrib>Uhač, Mia</creatorcontrib><creatorcontrib>Illeš, Davor</creatorcontrib><creatorcontrib>Kovačević Pavičić, Daniela</creatorcontrib><creatorcontrib>Simonić-Kocijan, Sunčana</creatorcontrib><title>P 2 Y 2 Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats</title><title>Journal of pain research</title><addtitle>J Pain Res</addtitle><description>P
Y
receptors (P
Y
Rs) are among the various receptors that play an important role in nociception. The goal of this research was to investigate possible P
Y
R expression changes in the trigeminal ganglion (TRG) in bilateral masseter muscle (MM) hypersensitivity following unilateral MM inflammation. The impact of unilateral intramasseteric administration of P
Y
R antagonist on bilateral MM hypersensitivity was also explored.
Bilateral MM hypersensitivity was provoked by unilateral intramasseteric injection of complete Freund's adjuvant (CFA). The head withdrawal threshold (HWT) was assessed bilaterally 4 days later. Bilateral TRG and MM isolation were followed, and quantitative real-time polymerase chain reaction (qRT-PCR) and histopathological analysis were carried out on these tissues, respectively. The involvement of P
Y
Rs in nocifensive behavior was evaluated by administering two doses of P
Y
R antagonist AR-C118925 (0.2 or 1 mg/100 μL) in inflamed MM 4 days post-CFA administration. Bilateral HWT was assessed at different time points following antagonist injection.
qRT-PCR analysis demonstrated P
Y
R up-regulation in TRG ipsilateral to the site of CFA administration. Compared to the controls, both doses of AR-C118925 injected ipsilateral to the TRG increased the bilateral HWT at 30, 60, 90, and 120 minutes after antagonist administration.
The findings suggest that P
Y
Rs may affect MM inflammatory hypersensitivity owing to its up-regulation in the TRG in MM inflammatory pain states.</description><issn>1178-7090</issn><issn>1178-7090</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpNkE1LxDAQhoMo7rp68i65S9dMkqbpURZ1lS0uVQ-eSppOMLIfJekK_fdWdhUPw8wLD8PLQ8glsCkHmd08LcvpCxe5FnBExgCZTjKWs-N_94icxfjJmNI8h1MyEjzVkGZyTIol5fR9mBIttt02RFpg402HtDAxYoeBFrtoV0NG-2E23poVnfcthoib6Dv_5bue-g0tTRfPyYkzq4gXhz0hb_d3r7N5snh-eJzdLhILQkMia2OFNZrXqHiulQZt0yxthkZDYy6dVsII7STLlayVdKJGlzKVGgDtIBcTcr3_a8M2xoCuaoNfm9BXwKofKdUgpTpIGeirPd3u6jU2f-yvBfENDQNa2g</recordid><startdate>202006</startdate><enddate>202006</enddate><creator>Tariba Knežević, Petra</creator><creator>Vukman, Robert</creator><creator>Uhač, Mia</creator><creator>Illeš, Davor</creator><creator>Kovačević Pavičić, Daniela</creator><creator>Simonić-Kocijan, Sunčana</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-6333-8533</orcidid><orcidid>https://orcid.org/0000-0002-8323-6515</orcidid><orcidid>https://orcid.org/0000-0002-0671-9820</orcidid><orcidid>https://orcid.org/0000-0002-1360-3279</orcidid><orcidid>https://orcid.org/0000-0002-8117-9203</orcidid><orcidid>https://orcid.org/0000-0002-3961-1728</orcidid></search><sort><creationdate>202006</creationdate><title>P 2 Y 2 Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats</title><author>Tariba Knežević, Petra ; Vukman, Robert ; Uhač, Mia ; Illeš, Davor ; Kovačević Pavičić, Daniela ; Simonić-Kocijan, Sunčana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1381-4bac3ca82be62986818c575d57417824f863a38f40964b64f3bef5065a118f193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tariba Knežević, Petra</creatorcontrib><creatorcontrib>Vukman, Robert</creatorcontrib><creatorcontrib>Uhač, Mia</creatorcontrib><creatorcontrib>Illeš, Davor</creatorcontrib><creatorcontrib>Kovačević Pavičić, Daniela</creatorcontrib><creatorcontrib>Simonić-Kocijan, Sunčana</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of pain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tariba Knežević, Petra</au><au>Vukman, Robert</au><au>Uhač, Mia</au><au>Illeš, Davor</au><au>Kovačević Pavičić, Daniela</au><au>Simonić-Kocijan, Sunčana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P 2 Y 2 Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats</atitle><jtitle>Journal of pain research</jtitle><addtitle>J Pain Res</addtitle><date>2020-06</date><risdate>2020</risdate><volume>13</volume><spage>1323</spage><epage>1333</epage><pages>1323-1333</pages><issn>1178-7090</issn><eissn>1178-7090</eissn><abstract>P
Y
receptors (P
Y
Rs) are among the various receptors that play an important role in nociception. The goal of this research was to investigate possible P
Y
R expression changes in the trigeminal ganglion (TRG) in bilateral masseter muscle (MM) hypersensitivity following unilateral MM inflammation. The impact of unilateral intramasseteric administration of P
Y
R antagonist on bilateral MM hypersensitivity was also explored.
Bilateral MM hypersensitivity was provoked by unilateral intramasseteric injection of complete Freund's adjuvant (CFA). The head withdrawal threshold (HWT) was assessed bilaterally 4 days later. Bilateral TRG and MM isolation were followed, and quantitative real-time polymerase chain reaction (qRT-PCR) and histopathological analysis were carried out on these tissues, respectively. The involvement of P
Y
Rs in nocifensive behavior was evaluated by administering two doses of P
Y
R antagonist AR-C118925 (0.2 or 1 mg/100 μL) in inflamed MM 4 days post-CFA administration. Bilateral HWT was assessed at different time points following antagonist injection.
qRT-PCR analysis demonstrated P
Y
R up-regulation in TRG ipsilateral to the site of CFA administration. Compared to the controls, both doses of AR-C118925 injected ipsilateral to the TRG increased the bilateral HWT at 30, 60, 90, and 120 minutes after antagonist administration.
The findings suggest that P
Y
Rs may affect MM inflammatory hypersensitivity owing to its up-regulation in the TRG in MM inflammatory pain states.</abstract><cop>New Zealand</cop><pmid>32581574</pmid><doi>10.2147/JPR.S239831</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-6333-8533</orcidid><orcidid>https://orcid.org/0000-0002-8323-6515</orcidid><orcidid>https://orcid.org/0000-0002-0671-9820</orcidid><orcidid>https://orcid.org/0000-0002-1360-3279</orcidid><orcidid>https://orcid.org/0000-0002-8117-9203</orcidid><orcidid>https://orcid.org/0000-0002-3961-1728</orcidid><oa>free_for_read</oa></addata></record> |
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source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; Dove Press Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
title | P 2 Y 2 Receptors Mediate Masseter Muscle Mechanical Hypersensitivity in Rats |
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