Functional Role of Ile264 in CYP2C8: Mutations Affect Haem Incorporation and Catalytic Activity

The work described in this study aimed to express CYP2C8 wild-type and mutant proteins in bacterial expression system and to use the expressed proteins to investigate the structural and functional consequences of a reported allele CYP2C8*4 (carrying Ile264Met substitution) on protein activity. Ile26...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	DRUG METABOLISM AND PHARMACOKINETICS 2008-01, Vol.23 (3), p.165-174
Hauptverfasser:	Singh, Rajinder, Ting, Jonathan G., Pan, Yan, Teh, Lay Kek, Ismail, Rusli, Ong, Chin Eng
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Animals Aryl Hydrocarbon Hydroxylases - genetics Aryl Hydrocarbon Hydroxylases - metabolism Catalysis Catalytic Domain Cloning, Molecular CYP2C84 Cytochrome P-450 CYP2C8 DNA Primers Endopeptidase K - metabolism Enzyme Stability Escherichia coli - genetics genetic polymorphism Heme - metabolism Humans Isoleucine - genetics Isoleucine - physiology Mutagenesis, Site-Directed NADPH-Ferrihemoprotein Reductase - genetics paclitaxel Paclitaxel - metabolism Paclitaxel - pharmacology pharmacogenomics Polymorphism, Genetic Protein Folding Rats site-directed mutagenesis Substrate Specificity Tolbutamide - metabolism Tolbutamide - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!