Changes in Biochemical Parameters, Antioxidative Enzymes and Histopathology of Liver Induced by Cadmium (Cd) and Chlorpyrifos (CPF) in Wistar Rats

Background: Cd and CPF intoxication may occur directly through drinking water. Since the population tend to receive combination of multiple intoxicants through environment contamination, there is need for conducting studies to assess the impact of individual and combined environmental pollutants. The present research work was designed to study hepatotoxicity induced by Cd, CPF and their combination.Methods: The experiment was carried out for 28 days in Wistar rats. G1: Control. G-2:CdCl2 @ 22.5mg/ kg b.wt / oral. G3: CPF @ 25 mg/ kg b.wt /per oral. G4:CdCl2@22.5 mg + CPF @ 25 mg/ kg b.wt /per oral. Biochemical parameters ware estimated from serum and liver samples were processed for tissue antioxidative parameters and histopathological examination. Result: Higher mean values of AST, ALT, ALP and lower liver GSH and SOD were observed in G2, 3 and 4 on 15th and 29th day when compared with G1. Liver in G2 and 3 showed mild degenerative changes, areas of necrosis and loss of architecture. In G4, lesions were moderate in severity. In addition, moderate perivascular fibrosis of portal triad was observed. The effects in combined group were severe than individual groups due to synergistic action of the combined pollutants.