Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold
Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the bioc...
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| Veröffentlicht in: | Acta biochimica Polonica 2015, Vol.62 (2), p.311-316 |
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| creator | Deptuła, Tobiasz Warowicka, Alicja Woźniak, Anna Grzeszkowiak, Mikołaj Jarzębski, Maciej Bednarowicz, Magdalena Patkowski, Adam Słomski, Ryszard |
| description | Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth. |
| doi_str_mv | 10.18388/abp.2015_1007 |
| format | Article |
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These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. 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These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth.</description><subject>Acrylamides - chemistry</subject><subject>Biocompatible Materials</subject><subject>Cell Survival</subject><subject>HEK293 Cells - drug effects</subject><subject>HeLa Cells - drug effects</subject><subject>Humans</subject><subject>Materials Testing</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Fluorescence</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - toxicity</subject><subject>Tissue Scaffolds</subject><issn>0001-527X</issn><issn>1734-154X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo7rp69Sj5A12Tpt20R1n8gkUvCnsrk3SCkbYJSRR79o8b_LrMwMz7DMxDyDlna96IprkE5dcl43XHGZMHZMmlqApeV_tDsmSM8aIu5X5BTmJ8ZawUvK2OyaKs24aJWi7J53ZOLrkPq22aqTM0vWAYXREwejdF-47Uu2EeMVhNJ5ich5CsHjBSBRF76ib6UNjofHB-HkCHXEbbIzUuZDThlCwMFLwfrIZkcx4iBaqsixqMcUN_So4MDBHPfvuKPN9cP23vit3j7f32aldoIepUyL6UZsOkVkZIVEpCqwS2qm4aJfJrmHem4liallVMMC2FlJUSkEeb3oBYkfXPXR1cjAFN54MdIczZXfdts8s2u3-bGbj4AfybGrH_j__pE18fMnUg</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Deptuła, Tobiasz</creator><creator>Warowicka, Alicja</creator><creator>Woźniak, Anna</creator><creator>Grzeszkowiak, Mikołaj</creator><creator>Jarzębski, Maciej</creator><creator>Bednarowicz, Magdalena</creator><creator>Patkowski, Adam</creator><creator>Słomski, Ryszard</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2015</creationdate><title>Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold</title><author>Deptuła, Tobiasz ; Warowicka, Alicja ; Woźniak, Anna ; Grzeszkowiak, Mikołaj ; Jarzębski, Maciej ; Bednarowicz, Magdalena ; Patkowski, Adam ; Słomski, Ryszard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-7d27f607cbf37ebb7a9b3e9b588b3023e607f41e2f904030c73774b3a1e26dfa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acrylamides - chemistry</topic><topic>Biocompatible Materials</topic><topic>Cell Survival</topic><topic>HEK293 Cells - drug effects</topic><topic>HeLa Cells - drug effects</topic><topic>Humans</topic><topic>Materials Testing</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Fluorescence</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - toxicity</topic><topic>Tissue Scaffolds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deptuła, Tobiasz</creatorcontrib><creatorcontrib>Warowicka, Alicja</creatorcontrib><creatorcontrib>Woźniak, Anna</creatorcontrib><creatorcontrib>Grzeszkowiak, Mikołaj</creatorcontrib><creatorcontrib>Jarzębski, Maciej</creatorcontrib><creatorcontrib>Bednarowicz, Magdalena</creatorcontrib><creatorcontrib>Patkowski, Adam</creatorcontrib><creatorcontrib>Słomski, Ryszard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Acta biochimica Polonica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deptuła, Tobiasz</au><au>Warowicka, Alicja</au><au>Woźniak, Anna</au><au>Grzeszkowiak, Mikołaj</au><au>Jarzębski, Maciej</au><au>Bednarowicz, Magdalena</au><au>Patkowski, Adam</au><au>Słomski, Ryszard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold</atitle><jtitle>Acta biochimica Polonica</jtitle><addtitle>Acta Biochim Pol</addtitle><date>2015</date><risdate>2015</risdate><volume>62</volume><issue>2</issue><spage>311</spage><epage>316</epage><pages>311-316</pages><issn>0001-527X</issn><eissn>1734-154X</eissn><abstract>Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. 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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Acrylamides - chemistry Biocompatible Materials Cell Survival HEK293 Cells - drug effects HeLa Cells - drug effects Humans Materials Testing Microscopy, Electron, Scanning Microscopy, Fluorescence Nanoparticles - chemistry Nanoparticles - toxicity Tissue Scaffolds |
| title | Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold |
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