High-grade mutant OmpF induces decreased bacterial survival rate
OmpF plays very important roles in the influx of antibiotics and bacterial survival in the presence of antibiotics. However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF)...
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| Veröffentlicht in: | Acta biochimica Polonica 2014-01, Vol.61 (2), p.369 |
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| container_issue | 2 |
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| container_title | Acta biochimica Polonica |
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| creator | Zhao, Zhi-ping Liu, Ting-ting Zhang, Li Luo, Min Nie, Xin Li, Zai-xin Pan, Yu |
| description | OmpF plays very important roles in the influx of antibiotics and bacterial survival in the presence of antibiotics. However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence. mOmpF protein was successfully expressed. When grown in the presence of antibiotic, the bacterial survival rate decreased and the antibiotic inhibition zone became larger with the increase of the mOmpF. It was concluded that concentration of high-grade mutant mOmpF dramatically influenced the bacterial survival rate. The study presented here may provide insights into better understanding of the relationships between structure and function of OmpF. |
| doi_str_mv | 10.18388/abp.2014_1908 |
| format | Article |
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However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence. mOmpF protein was successfully expressed. When grown in the presence of antibiotic, the bacterial survival rate decreased and the antibiotic inhibition zone became larger with the increase of the mOmpF. It was concluded that concentration of high-grade mutant mOmpF dramatically influenced the bacterial survival rate. 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However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence. mOmpF protein was successfully expressed. When grown in the presence of antibiotic, the bacterial survival rate decreased and the antibiotic inhibition zone became larger with the increase of the mOmpF. It was concluded that concentration of high-grade mutant mOmpF dramatically influenced the bacterial survival rate. 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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Anti-Bacterial Agents - pharmacology Drug Resistance, Multiple, Bacterial - genetics Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Kanamycin - pharmacology Microbial Viability - genetics Molecular Sequence Data Mutation Porins - genetics Porins - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Alignment |
| title | High-grade mutant OmpF induces decreased bacterial survival rate |
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