Ribes fragrans Pall.: Supercritical CO2-extraction and complete plant metabolome

Supercritical fluid technology was used to obtain bioactive substances from leaves of Ribes fragrans Pallas. Supercritical fluid technology showed the strongest extractive effect, which helped to extract the maximum amount of bioactive substances. Several experimental conditions were investigated in the pressure range 50–300 bar, with the used volume of co-solvent ethanol in the amount of 2.5 % in the liquid phase at a temperature in the range of 31–70°C. The most effective extraction conditions were: pressure 200 Bar and temperature 55°C. The CO2-extract of leaves of R. fragrans contain various polyphenolic compounds and compounds of other chemical groups with valuable biological activity. For the first time, the metabolome of supercritical extracts of R. fragrans leaves was studied in this research. Using the method of supercritical extraction of plant matrices with the method of tandem mass spectrometry, it was possible to tentatively identify 79 bioactive compounds, of which 53 compounds are of polyphenol group. 20 chemical compounds from polyphenol group and 12 chemical compounds from other chemical groups were identified for the first time in genus Ribes.