Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D
Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mie...
Gespeichert in:
| Veröffentlicht in: | Ciência rural 2008-09, Vol.39 (1), p.269-271 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 271 |
|---|---|
| container_issue | 1 |
| container_start_page | 269 |
| container_title | Ciência rural |
| container_volume | 39 |
| creator | Freitas, Theonys Diógenes Heneine, Luis Guilherme Dias Salvarani, Felipe Masiero Silva, Rodrigo Otávio Silveira Lima, Catarina Guimarães Rocha Dourado Pires, Prhiscylla Sadanã Assis, Ronnie Antunes de Lobato, Francisco Carlos Faria |
| description | Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas.
Clostridium perfringens type D is the aetiological agent of enterotoxemia in ruminants. The disease is caused by epsilon toxin characterized by cardiac, pulmonary, kidney and brain edema. Monoclonal antibodies were produced by using myeloma cell line P3-X63-Ag8 653 fused with spleen cells from Balb/c mice, immunized with epsilon toxoid of C. perfringens type D. Six hybrids were established secreting monoclonal antibodies of the IgM class and IgG3 subclass. |
| doi_str_mv | 10.1590/S0103-84782008005000051 |
| format | Article |
| fullrecord | <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1590_S0103_84782008005000051</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1590_S0103_84782008005000051</sourcerecordid><originalsourceid>FETCH-crossref_primary_10_1590_S0103_847820080050000513</originalsourceid><addsrcrecordid>eNqdj81OwzAQhC0EEuXnGdgXCKybpEnPpYgjEr1bluOgRYnXWrsS8DocUJ8jL0YLHJC4MZcZzWgOn1JXGq91vcSbR9RYFm3VtHPEFrHGvWp9pGZ60bRFVS8Xx7_yqTpL6Rlx3pRVNVPxQbjbTh_TO4MHZ8W67IXe7HfVebAhk2OJnGDkwG7gYCmB45DFQuYXChamXUy0Xw6H1cApC3W0HSF66YXCkw8JNhQZbi_USW-H5C9__Fw1d-vN6r5wwimJ700UGq28Go3mAGi-AM0fwPL_z09gAl9C</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Freitas, Theonys Diógenes ; Heneine, Luis Guilherme Dias ; Salvarani, Felipe Masiero ; Silva, Rodrigo Otávio Silveira ; Lima, Catarina Guimarães Rocha Dourado ; Pires, Prhiscylla Sadanã ; Assis, Ronnie Antunes de ; Lobato, Francisco Carlos Faria</creator><creatorcontrib>Freitas, Theonys Diógenes ; Heneine, Luis Guilherme Dias ; Salvarani, Felipe Masiero ; Silva, Rodrigo Otávio Silveira ; Lima, Catarina Guimarães Rocha Dourado ; Pires, Prhiscylla Sadanã ; Assis, Ronnie Antunes de ; Lobato, Francisco Carlos Faria</creatorcontrib><description>Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas.
Clostridium perfringens type D is the aetiological agent of enterotoxemia in ruminants. The disease is caused by epsilon toxin characterized by cardiac, pulmonary, kidney and brain edema. Monoclonal antibodies were produced by using myeloma cell line P3-X63-Ag8 653 fused with spleen cells from Balb/c mice, immunized with epsilon toxoid of C. perfringens type D. Six hybrids were established secreting monoclonal antibodies of the IgM class and IgG3 subclass.</description><identifier>ISSN: 1678-4596</identifier><identifier>EISSN: 1678-4596</identifier><identifier>DOI: 10.1590/S0103-84782008005000051</identifier><language>eng</language><ispartof>Ciência rural, 2008-09, Vol.39 (1), p.269-271</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-crossref_primary_10_1590_S0103_847820080050000513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Freitas, Theonys Diógenes</creatorcontrib><creatorcontrib>Heneine, Luis Guilherme Dias</creatorcontrib><creatorcontrib>Salvarani, Felipe Masiero</creatorcontrib><creatorcontrib>Silva, Rodrigo Otávio Silveira</creatorcontrib><creatorcontrib>Lima, Catarina Guimarães Rocha Dourado</creatorcontrib><creatorcontrib>Pires, Prhiscylla Sadanã</creatorcontrib><creatorcontrib>Assis, Ronnie Antunes de</creatorcontrib><creatorcontrib>Lobato, Francisco Carlos Faria</creatorcontrib><title>Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D</title><title>Ciência rural</title><description>Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas.
Clostridium perfringens type D is the aetiological agent of enterotoxemia in ruminants. The disease is caused by epsilon toxin characterized by cardiac, pulmonary, kidney and brain edema. Monoclonal antibodies were produced by using myeloma cell line P3-X63-Ag8 653 fused with spleen cells from Balb/c mice, immunized with epsilon toxoid of C. perfringens type D. Six hybrids were established secreting monoclonal antibodies of the IgM class and IgG3 subclass.</description><issn>1678-4596</issn><issn>1678-4596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqdj81OwzAQhC0EEuXnGdgXCKybpEnPpYgjEr1bluOgRYnXWrsS8DocUJ8jL0YLHJC4MZcZzWgOn1JXGq91vcSbR9RYFm3VtHPEFrHGvWp9pGZ60bRFVS8Xx7_yqTpL6Rlx3pRVNVPxQbjbTh_TO4MHZ8W67IXe7HfVebAhk2OJnGDkwG7gYCmB45DFQuYXChamXUy0Xw6H1cApC3W0HSF66YXCkw8JNhQZbi_USW-H5C9__Fw1d-vN6r5wwimJ700UGq28Go3mAGi-AM0fwPL_z09gAl9C</recordid><startdate>20080904</startdate><enddate>20080904</enddate><creator>Freitas, Theonys Diógenes</creator><creator>Heneine, Luis Guilherme Dias</creator><creator>Salvarani, Felipe Masiero</creator><creator>Silva, Rodrigo Otávio Silveira</creator><creator>Lima, Catarina Guimarães Rocha Dourado</creator><creator>Pires, Prhiscylla Sadanã</creator><creator>Assis, Ronnie Antunes de</creator><creator>Lobato, Francisco Carlos Faria</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20080904</creationdate><title>Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D</title><author>Freitas, Theonys Diógenes ; Heneine, Luis Guilherme Dias ; Salvarani, Felipe Masiero ; Silva, Rodrigo Otávio Silveira ; Lima, Catarina Guimarães Rocha Dourado ; Pires, Prhiscylla Sadanã ; Assis, Ronnie Antunes de ; Lobato, Francisco Carlos Faria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-crossref_primary_10_1590_S0103_847820080050000513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Freitas, Theonys Diógenes</creatorcontrib><creatorcontrib>Heneine, Luis Guilherme Dias</creatorcontrib><creatorcontrib>Salvarani, Felipe Masiero</creatorcontrib><creatorcontrib>Silva, Rodrigo Otávio Silveira</creatorcontrib><creatorcontrib>Lima, Catarina Guimarães Rocha Dourado</creatorcontrib><creatorcontrib>Pires, Prhiscylla Sadanã</creatorcontrib><creatorcontrib>Assis, Ronnie Antunes de</creatorcontrib><creatorcontrib>Lobato, Francisco Carlos Faria</creatorcontrib><collection>CrossRef</collection><jtitle>Ciência rural</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Freitas, Theonys Diógenes</au><au>Heneine, Luis Guilherme Dias</au><au>Salvarani, Felipe Masiero</au><au>Silva, Rodrigo Otávio Silveira</au><au>Lima, Catarina Guimarães Rocha Dourado</au><au>Pires, Prhiscylla Sadanã</au><au>Assis, Ronnie Antunes de</au><au>Lobato, Francisco Carlos Faria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D</atitle><jtitle>Ciência rural</jtitle><date>2008-09-04</date><risdate>2008</risdate><volume>39</volume><issue>1</issue><spage>269</spage><epage>271</epage><pages>269-271</pages><issn>1678-4596</issn><eissn>1678-4596</eissn><abstract>Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas.
Clostridium perfringens type D is the aetiological agent of enterotoxemia in ruminants. The disease is caused by epsilon toxin characterized by cardiac, pulmonary, kidney and brain edema. Monoclonal antibodies were produced by using myeloma cell line P3-X63-Ag8 653 fused with spleen cells from Balb/c mice, immunized with epsilon toxoid of C. perfringens type D. Six hybrids were established secreting monoclonal antibodies of the IgM class and IgG3 subclass.</abstract><doi>10.1590/S0103-84782008005000051</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1678-4596 |
| ispartof | Ciência rural, 2008-09, Vol.39 (1), p.269-271 |
| issn | 1678-4596 1678-4596 |
| language | eng |
| recordid | cdi_crossref_primary_10_1590_S0103_84782008005000051 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| title | Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T12%3A31%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Produ%C3%A7%C3%A3o%20e%20caracteriza%C3%A7%C3%A3o%20de%20anticorpos%20monoclonais%20contra%20toxina%20%C3%A9psilon%20de%20Clostridium%20perfringens%20Tipo%20D&rft.jtitle=Ci%C3%AAncia%20rural&rft.au=Freitas,%20Theonys%20Di%C3%B3genes&rft.date=2008-09-04&rft.volume=39&rft.issue=1&rft.spage=269&rft.epage=271&rft.pages=269-271&rft.issn=1678-4596&rft.eissn=1678-4596&rft_id=info:doi/10.1590/S0103-84782008005000051&rft_dat=%3Ccrossref%3E10_1590_S0103_84782008005000051%3C/crossref%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |