Biopolymer Xanthan: A New Adjuvant for DNA Vaccines
Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the p...
Gespeichert in:
| Veröffentlicht in: | Brazilian Archives of Biology and Technology 2020-01, Vol.63 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | Brazilian Archives of Biology and Technology |
| container_volume | 63 |
| creator | Silveira, Marcelle Moura Conceição, Fabricio Rochedo Mendonça, Marcelo Moreira, Gustavo Marçal Schmidt Garcia Cunha, Carlos Eduardo Pouey da Rizzi, Caroline Hartwig, Daiane Drawanz Moreira, Angelita da Silveira Vendrusculo, Claire Tondo Moreira, Ângela Nunes |
| description | Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan. |
| doi_str_mv | 10.1590/1678-4324-2020190090 |
| format | Article |
| fullrecord | <record><control><sourceid>doaj_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1590_1678_4324_2020190090</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_f05a0745a98241ecaf48bd5e238b1858</doaj_id><sourcerecordid>oai_doaj_org_article_f05a0745a98241ecaf48bd5e238b1858</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-87a54184ffdf81fe64771ef5850961108b878e955726664460aa22a4da90c0793</originalsourceid><addsrcrecordid>eNpFkM1OwkAURidGExF9Axd9geq90zt_7iqKkhDcqHE3ubQzWgKUTFHD2wticPV9OYuzOEJcIlyhcnCN2ticCkm5BAnoABwcid4BH2-_Qp1bh8WpOOu6GQCSRuqJ4rZpV-18swgpe-Pl-oOXN1mZTcJ3Vtazz68tymKbsrtJmb1yVTXL0J2Lk8jzLlz8bV-8DO-fB4_5-OlhNCjHeUUg17k1rAgtxVhHizFoMgZDVFaB04hgp9bY4JQyUmtNpIFZSqaaHVRgXNEXo723bnnmV6lZcNr4lhv_C9r07jmtm2oefATFYEixs5IwVBzJTmsVZGGnaJXdumjvqlLbdSnEgw_B7yL6XS2_q-X_IxY_y5Jg0w</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Biopolymer Xanthan: A New Adjuvant for DNA Vaccines</title><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Silveira, Marcelle Moura ; Conceição, Fabricio Rochedo ; Mendonça, Marcelo ; Moreira, Gustavo Marçal Schmidt Garcia ; Cunha, Carlos Eduardo Pouey da ; Rizzi, Caroline ; Hartwig, Daiane Drawanz ; Moreira, Angelita da Silveira ; Vendrusculo, Claire Tondo ; Moreira, Ângela Nunes</creator><creatorcontrib>Silveira, Marcelle Moura ; Conceição, Fabricio Rochedo ; Mendonça, Marcelo ; Moreira, Gustavo Marçal Schmidt Garcia ; Cunha, Carlos Eduardo Pouey da ; Rizzi, Caroline ; Hartwig, Daiane Drawanz ; Moreira, Angelita da Silveira ; Vendrusculo, Claire Tondo ; Moreira, Ângela Nunes</creatorcontrib><description>Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.</description><identifier>ISSN: 1516-8913</identifier><identifier>EISSN: 1678-4324</identifier><identifier>DOI: 10.1590/1678-4324-2020190090</identifier><language>eng</language><publisher>Instituto de Tecnologia do Paraná (Tecpar)</publisher><subject>immunologic adjuvant ; Recombinant DNA vaccine ; xanthan gum</subject><ispartof>Brazilian Archives of Biology and Technology, 2020-01, Vol.63</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-87a54184ffdf81fe64771ef5850961108b878e955726664460aa22a4da90c0793</citedby><cites>FETCH-LOGICAL-c402t-87a54184ffdf81fe64771ef5850961108b878e955726664460aa22a4da90c0793</cites><orcidid>0000-0003-3604-0832 ; 0000-0003-0357-5988 ; 0000-0002-1072-4374 ; 0000-0001-5810-9592 ; 0000-0001-9199-0764 ; 0000-0002-3790-6557 ; 0000-0001-8711-6521 ; 0000-0001-5104-5039 ; 0000-0001-5347-8559 ; 0000-0001-7415-457X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Silveira, Marcelle Moura</creatorcontrib><creatorcontrib>Conceição, Fabricio Rochedo</creatorcontrib><creatorcontrib>Mendonça, Marcelo</creatorcontrib><creatorcontrib>Moreira, Gustavo Marçal Schmidt Garcia</creatorcontrib><creatorcontrib>Cunha, Carlos Eduardo Pouey da</creatorcontrib><creatorcontrib>Rizzi, Caroline</creatorcontrib><creatorcontrib>Hartwig, Daiane Drawanz</creatorcontrib><creatorcontrib>Moreira, Angelita da Silveira</creatorcontrib><creatorcontrib>Vendrusculo, Claire Tondo</creatorcontrib><creatorcontrib>Moreira, Ângela Nunes</creatorcontrib><title>Biopolymer Xanthan: A New Adjuvant for DNA Vaccines</title><title>Brazilian Archives of Biology and Technology</title><description>Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.</description><subject>immunologic adjuvant</subject><subject>Recombinant DNA vaccine</subject><subject>xanthan gum</subject><issn>1516-8913</issn><issn>1678-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpFkM1OwkAURidGExF9Axd9geq90zt_7iqKkhDcqHE3ubQzWgKUTFHD2wticPV9OYuzOEJcIlyhcnCN2ticCkm5BAnoABwcid4BH2-_Qp1bh8WpOOu6GQCSRuqJ4rZpV-18swgpe-Pl-oOXN1mZTcJ3Vtazz68tymKbsrtJmb1yVTXL0J2Lk8jzLlz8bV-8DO-fB4_5-OlhNCjHeUUg17k1rAgtxVhHizFoMgZDVFaB04hgp9bY4JQyUmtNpIFZSqaaHVRgXNEXo723bnnmV6lZcNr4lhv_C9r07jmtm2oefATFYEixs5IwVBzJTmsVZGGnaJXdumjvqlLbdSnEgw_B7yL6XS2_q-X_IxY_y5Jg0w</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Silveira, Marcelle Moura</creator><creator>Conceição, Fabricio Rochedo</creator><creator>Mendonça, Marcelo</creator><creator>Moreira, Gustavo Marçal Schmidt Garcia</creator><creator>Cunha, Carlos Eduardo Pouey da</creator><creator>Rizzi, Caroline</creator><creator>Hartwig, Daiane Drawanz</creator><creator>Moreira, Angelita da Silveira</creator><creator>Vendrusculo, Claire Tondo</creator><creator>Moreira, Ângela Nunes</creator><general>Instituto de Tecnologia do Paraná (Tecpar)</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3604-0832</orcidid><orcidid>https://orcid.org/0000-0003-0357-5988</orcidid><orcidid>https://orcid.org/0000-0002-1072-4374</orcidid><orcidid>https://orcid.org/0000-0001-5810-9592</orcidid><orcidid>https://orcid.org/0000-0001-9199-0764</orcidid><orcidid>https://orcid.org/0000-0002-3790-6557</orcidid><orcidid>https://orcid.org/0000-0001-8711-6521</orcidid><orcidid>https://orcid.org/0000-0001-5104-5039</orcidid><orcidid>https://orcid.org/0000-0001-5347-8559</orcidid><orcidid>https://orcid.org/0000-0001-7415-457X</orcidid></search><sort><creationdate>20200101</creationdate><title>Biopolymer Xanthan: A New Adjuvant for DNA Vaccines</title><author>Silveira, Marcelle Moura ; Conceição, Fabricio Rochedo ; Mendonça, Marcelo ; Moreira, Gustavo Marçal Schmidt Garcia ; Cunha, Carlos Eduardo Pouey da ; Rizzi, Caroline ; Hartwig, Daiane Drawanz ; Moreira, Angelita da Silveira ; Vendrusculo, Claire Tondo ; Moreira, Ângela Nunes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-87a54184ffdf81fe64771ef5850961108b878e955726664460aa22a4da90c0793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>immunologic adjuvant</topic><topic>Recombinant DNA vaccine</topic><topic>xanthan gum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silveira, Marcelle Moura</creatorcontrib><creatorcontrib>Conceição, Fabricio Rochedo</creatorcontrib><creatorcontrib>Mendonça, Marcelo</creatorcontrib><creatorcontrib>Moreira, Gustavo Marçal Schmidt Garcia</creatorcontrib><creatorcontrib>Cunha, Carlos Eduardo Pouey da</creatorcontrib><creatorcontrib>Rizzi, Caroline</creatorcontrib><creatorcontrib>Hartwig, Daiane Drawanz</creatorcontrib><creatorcontrib>Moreira, Angelita da Silveira</creatorcontrib><creatorcontrib>Vendrusculo, Claire Tondo</creatorcontrib><creatorcontrib>Moreira, Ângela Nunes</creatorcontrib><collection>CrossRef</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Brazilian Archives of Biology and Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silveira, Marcelle Moura</au><au>Conceição, Fabricio Rochedo</au><au>Mendonça, Marcelo</au><au>Moreira, Gustavo Marçal Schmidt Garcia</au><au>Cunha, Carlos Eduardo Pouey da</au><au>Rizzi, Caroline</au><au>Hartwig, Daiane Drawanz</au><au>Moreira, Angelita da Silveira</au><au>Vendrusculo, Claire Tondo</au><au>Moreira, Ângela Nunes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biopolymer Xanthan: A New Adjuvant for DNA Vaccines</atitle><jtitle>Brazilian Archives of Biology and Technology</jtitle><date>2020-01-01</date><risdate>2020</risdate><volume>63</volume><issn>1516-8913</issn><eissn>1678-4324</eissn><abstract>Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.</abstract><pub>Instituto de Tecnologia do Paraná (Tecpar)</pub><doi>10.1590/1678-4324-2020190090</doi><orcidid>https://orcid.org/0000-0003-3604-0832</orcidid><orcidid>https://orcid.org/0000-0003-0357-5988</orcidid><orcidid>https://orcid.org/0000-0002-1072-4374</orcidid><orcidid>https://orcid.org/0000-0001-5810-9592</orcidid><orcidid>https://orcid.org/0000-0001-9199-0764</orcidid><orcidid>https://orcid.org/0000-0002-3790-6557</orcidid><orcidid>https://orcid.org/0000-0001-8711-6521</orcidid><orcidid>https://orcid.org/0000-0001-5104-5039</orcidid><orcidid>https://orcid.org/0000-0001-5347-8559</orcidid><orcidid>https://orcid.org/0000-0001-7415-457X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1516-8913 |
| ispartof | Brazilian Archives of Biology and Technology, 2020-01, Vol.63 |
| issn | 1516-8913 1678-4324 |
| language | eng |
| recordid | cdi_crossref_primary_10_1590_1678_4324_2020190090 |
| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | immunologic adjuvant Recombinant DNA vaccine xanthan gum |
| title | Biopolymer Xanthan: A New Adjuvant for DNA Vaccines |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T14%3A16%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-doaj_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biopolymer%20Xanthan:%20A%20New%20Adjuvant%20for%20DNA%20Vaccines&rft.jtitle=Brazilian%20Archives%20of%20Biology%20and%20Technology&rft.au=Silveira,%20Marcelle%20Moura&rft.date=2020-01-01&rft.volume=63&rft.issn=1516-8913&rft.eissn=1678-4324&rft_id=info:doi/10.1590/1678-4324-2020190090&rft_dat=%3Cdoaj_cross%3Eoai_doaj_org_article_f05a0745a98241ecaf48bd5e238b1858%3C/doaj_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_doaj_id=oai_doaj_org_article_f05a0745a98241ecaf48bd5e238b1858&rfr_iscdi=true |