Biopolymer Xanthan: A New Adjuvant for DNA Vaccines

Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the p...

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Veröffentlicht in:	Brazilian Archives of Biology and Technology 2020-01, Vol.63
Hauptverfasser:	Silveira, Marcelle Moura, Conceição, Fabricio Rochedo, Mendonça, Marcelo, Moreira, Gustavo Marçal Schmidt Garcia, Cunha, Carlos Eduardo Pouey da, Rizzi, Caroline, Hartwig, Daiane Drawanz, Moreira, Angelita da Silveira, Vendrusculo, Claire Tondo, Moreira, Ângela Nunes
Format:	Artikel
Sprache:	eng
Schlagworte:	immunologic adjuvant Recombinant DNA vaccine xanthan gum
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Zusammenfassung:	Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.
ISSN:	1516-8913 1678-4324
DOI:	10.1590/1678-4324-2020190090