THE RESURRECTION PLANT TRIPOGON SPICATUS (POACEAE) HARBORS A DIVERSITY OF PLANT GROWTH PROMOTING BACTERIA IN NORTHEASTERN BRAZILIAN CAATINGA
Plant species that naturally occur in the Brazilian Caatinga(xeric shrubland) adapt in several ways to these harsh conditions, and that can be exploited to increase crop production. Among the strategic adaptations to confront low water availability, desiccation tolerance stands out. Up to now, the a...
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| creator | Fernandes-Júnior, Paulo Ivan Aidar, Saulo de Tarso Morgante, Carolina Vianna Gava, Carlos Alberto Tuão Zilli, Jerri Édson Souza, Layane Silva Barbosa de Marinho, Rita de Cássia Nunes Nóbrega, Rafaela Simão Abrahão Brasil, Marivaine da Silva Seido, Sirando Lima Martins, Lindete Míria Vieira |
| description | Plant species that naturally occur in the Brazilian Caatinga(xeric shrubland) adapt in several ways to these harsh conditions, and that can be exploited to increase crop production. Among the strategic adaptations to confront low water availability, desiccation tolerance stands out. Up to now, the association of those species with beneficial soil microorganisms is not well understood. The aim of this study was to characterize Tripogon spicatusdiazotrophic bacterial isolates from the Caatingabiome and evaluate their ability to promote plant growth in rice. Sixteen bacterial isolates were studied in regard to their taxonomic position by partial sequencing of the 16S rRNA gene, putative diazotrophic capacity, in vitro indole-acetic acid (IAA) production and calcium phosphate solubilization, metabolism of nine different C sources in semi-solid media, tolerance to different concentrations of NaCl to pHs and intrinsic resistance to nine antibiotics. Finally, the ability of the bacterial isolates to promote plant growth was evaluated using rice (Oryza sativa) as a model plant. Among the 16 isolates evaluated, eight of them were classified as Enterobacteriaceae members, related to Enterobacter andPantoeagenera. Six other bacteria were related toBacillus, and the remaining two were related toRhizobiumand Stenotrophomonas.The evaluation of total N incorporation into the semi-solid medium indicated that all the bacteria studied have putative diazotrophic capacity. Two bacteria were able to produce more IAA than that observed for the strain BR 11175Tof Herbaspirillum seropedicae.Bacterial isolates were also able to form a microaerophilic pellicle in a semi-solid medium supplemented with different NaCl concentrations up to 1.27 mol L-1. Intrinsic resistance to antibiotics and the metabolism of different C sources indicated a great variation in physiological profile. Seven isolates were able to promote rice growth, and two bacteria were more efficient than the reference strainAzospirillum brasilense, Ab-V5. The results indicate the potential of T. spicatus as native plant source of plant growth promoting bacteria.
As espécies vegetais que ocorrem naturalmente na Caatinga brasileira apresentam diversas adaptações às condições adversas que podem ser exploradas para aumentar a produção vegetal. Entre essas estratégias adaptativas para o enfrentamento da baixa disponibilidade hídrica, a tolerância à dessecação pode ser destacada. Até o momento, a associação entre essas espé |
| doi_str_mv | 10.1590/01000683rbcs20140646 |
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As espécies vegetais que ocorrem naturalmente na Caatinga brasileira apresentam diversas adaptações às condições adversas que podem ser exploradas para aumentar a produção vegetal. Entre essas estratégias adaptativas para o enfrentamento da baixa disponibilidade hídrica, a tolerância à dessecação pode ser destacada. Até o momento, a associação entre essas espécies e os microrganismos benéficos do solo não é bem entendida. Os objetivos deste trabalho foram caracterizar bactérias diazotróficas de Tripogon spicatus e avaliar a capacidade de promoção do crescimento delas em plantas de arroz. Dezesseis isolados bacterianos foram estudados quanto a seu posicionamento taxonômico por meio do sequenciamento parcial do gene 16S rRNA, potencial diazotrófico, produção de ácido indol acético (AIA) e solubilização de fosfato de cálcio in vitro, metabolismo de nove diferentes fontes de C em meio semissólido, tolerância a diferentes concentrações de NaCl a pHs e resistência intrínseca a nove antibióticos. A habilidade em promover o crescimento vegetal foi avaliada utilizando o arroz (Oryza sativa) como planta-modelo. Dentre os 16 isolados avaliados, oito foram classificados como pertencentes à família Enterobacteriaceae, relacionados aos gêneros Enterobacter e Pantoea. Seis outros isolados foram relacionados a Bacillus e outros dois a Rhizobium e Stenotrophomonas. A avaliação da incorporação do N ao meio semissólido demonstrou o potencial diazotrófico de todos os isolados. Duas bactérias produziram mais AIA do que a estirpe BR 11175T de Herbaspirillum seropedicae. Isolados bacterianos foram capazes de formar película em meio semissólido suplementado com concentrações de NaCl de até 1.27 mol L-1. A resistência intrínseca a antibióticos e o metabolismo de diferentes fontes de C indicaram um perfil fisiológico muito variável. Sete isolados foram capazes de aumentar o crescimento das plantas de arroz, mais eficientemente do que a estirpe de referência Ab-V5 de Azospirillum brasilense. Os resultados indicaram o potencial de T. spicatus como uma espécie nativa fonte de bactérias promotoras de crescimento vegetal.</description><identifier>ISSN: 0100-0683</identifier><identifier>ISSN: 1806-9657</identifier><identifier>EISSN: 0100-0683</identifier><identifier>DOI: 10.1590/01000683rbcs20140646</identifier><language>eng</language><publisher>Sociedade Brasileira de Ciência do Solo</publisher><subject>bactérias diazotróficas ; fixação biológica do nitrogênio ; inoculante ; plantas tolerantes à dessecação ; Semiárido ; SOIL SCIENCE</subject><ispartof>Revista Brasileira de Ciência do Solo, 2015-08, Vol.39 (4), p.993-1002</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c305t-8f80ddfcb296b9fbf346270126a1ba70eaaa43165c3c5d380e0777a0721aa9813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,864,885,27924,27925</link.rule.ids></links><search><creatorcontrib>Fernandes-Júnior, Paulo Ivan</creatorcontrib><creatorcontrib>Aidar, Saulo de Tarso</creatorcontrib><creatorcontrib>Morgante, Carolina Vianna</creatorcontrib><creatorcontrib>Gava, Carlos Alberto Tuão</creatorcontrib><creatorcontrib>Zilli, Jerri Édson</creatorcontrib><creatorcontrib>Souza, Layane Silva Barbosa de</creatorcontrib><creatorcontrib>Marinho, Rita de Cássia Nunes</creatorcontrib><creatorcontrib>Nóbrega, Rafaela Simão Abrahão</creatorcontrib><creatorcontrib>Brasil, Marivaine da Silva</creatorcontrib><creatorcontrib>Seido, Sirando Lima</creatorcontrib><creatorcontrib>Martins, Lindete Míria Vieira</creatorcontrib><title>THE RESURRECTION PLANT TRIPOGON SPICATUS (POACEAE) HARBORS A DIVERSITY OF PLANT GROWTH PROMOTING BACTERIA IN NORTHEASTERN BRAZILIAN CAATINGA</title><title>Revista Brasileira de Ciência do Solo</title><addtitle>Rev. Bras. Ciênc. Solo</addtitle><description>Plant species that naturally occur in the Brazilian Caatinga(xeric shrubland) adapt in several ways to these harsh conditions, and that can be exploited to increase crop production. Among the strategic adaptations to confront low water availability, desiccation tolerance stands out. Up to now, the association of those species with beneficial soil microorganisms is not well understood. The aim of this study was to characterize Tripogon spicatusdiazotrophic bacterial isolates from the Caatingabiome and evaluate their ability to promote plant growth in rice. Sixteen bacterial isolates were studied in regard to their taxonomic position by partial sequencing of the 16S rRNA gene, putative diazotrophic capacity, in vitro indole-acetic acid (IAA) production and calcium phosphate solubilization, metabolism of nine different C sources in semi-solid media, tolerance to different concentrations of NaCl to pHs and intrinsic resistance to nine antibiotics. Finally, the ability of the bacterial isolates to promote plant growth was evaluated using rice (Oryza sativa) as a model plant. Among the 16 isolates evaluated, eight of them were classified as Enterobacteriaceae members, related to Enterobacter andPantoeagenera. Six other bacteria were related toBacillus, and the remaining two were related toRhizobiumand Stenotrophomonas.The evaluation of total N incorporation into the semi-solid medium indicated that all the bacteria studied have putative diazotrophic capacity. Two bacteria were able to produce more IAA than that observed for the strain BR 11175Tof Herbaspirillum seropedicae.Bacterial isolates were also able to form a microaerophilic pellicle in a semi-solid medium supplemented with different NaCl concentrations up to 1.27 mol L-1. Intrinsic resistance to antibiotics and the metabolism of different C sources indicated a great variation in physiological profile. Seven isolates were able to promote rice growth, and two bacteria were more efficient than the reference strainAzospirillum brasilense, Ab-V5. The results indicate the potential of T. spicatus as native plant source of plant growth promoting bacteria.
As espécies vegetais que ocorrem naturalmente na Caatinga brasileira apresentam diversas adaptações às condições adversas que podem ser exploradas para aumentar a produção vegetal. Entre essas estratégias adaptativas para o enfrentamento da baixa disponibilidade hídrica, a tolerância à dessecação pode ser destacada. Até o momento, a associação entre essas espécies e os microrganismos benéficos do solo não é bem entendida. Os objetivos deste trabalho foram caracterizar bactérias diazotróficas de Tripogon spicatus e avaliar a capacidade de promoção do crescimento delas em plantas de arroz. Dezesseis isolados bacterianos foram estudados quanto a seu posicionamento taxonômico por meio do sequenciamento parcial do gene 16S rRNA, potencial diazotrófico, produção de ácido indol acético (AIA) e solubilização de fosfato de cálcio in vitro, metabolismo de nove diferentes fontes de C em meio semissólido, tolerância a diferentes concentrações de NaCl a pHs e resistência intrínseca a nove antibióticos. A habilidade em promover o crescimento vegetal foi avaliada utilizando o arroz (Oryza sativa) como planta-modelo. Dentre os 16 isolados avaliados, oito foram classificados como pertencentes à família Enterobacteriaceae, relacionados aos gêneros Enterobacter e Pantoea. Seis outros isolados foram relacionados a Bacillus e outros dois a Rhizobium e Stenotrophomonas. A avaliação da incorporação do N ao meio semissólido demonstrou o potencial diazotrófico de todos os isolados. Duas bactérias produziram mais AIA do que a estirpe BR 11175T de Herbaspirillum seropedicae. Isolados bacterianos foram capazes de formar película em meio semissólido suplementado com concentrações de NaCl de até 1.27 mol L-1. A resistência intrínseca a antibióticos e o metabolismo de diferentes fontes de C indicaram um perfil fisiológico muito variável. Sete isolados foram capazes de aumentar o crescimento das plantas de arroz, mais eficientemente do que a estirpe de referência Ab-V5 de Azospirillum brasilense. Os resultados indicaram o potencial de T. spicatus como uma espécie nativa fonte de bactérias promotoras de crescimento vegetal.</description><subject>bactérias diazotróficas</subject><subject>fixação biológica do nitrogênio</subject><subject>inoculante</subject><subject>plantas tolerantes à dessecação</subject><subject>Semiárido</subject><subject>SOIL SCIENCE</subject><issn>0100-0683</issn><issn>1806-9657</issn><issn>0100-0683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNptUcFu1DAQjRCVKKV_wMFHOGwZx06cHL0h3bW0xJHjbQUXa-IkaFcLqZL20H_go3HYCnHgNDNP8948zYui9xRuaJLDJ6AAkGZsav0cA-WQ8vRVdLnAqwV__U__Jno7z0eAOIecX0a_7LYkpmz2xpSFVboi9U5Wllijar0JY1OrQtp9Qz7UWhalLD-SrTRrbRoiyWd1V5pG2a9E374QN0bf2y2pjf6irao2ZC0LWxoliapIpU24J5sAVGRt5De1U7IihZTLqnwXXQx4mvvrl3oV7W9LW2xXO70JLnYrzyB5XGVDBl03-DbO0zYf2oHxNBZA4xRpiwJ6ROSMpolnPulYBj0IIRBETBHzjLKrSJ11uxGP7mE6_MDp2Y14cH-AcfrucHo8-FPvMjowijSO-SA4sq7liF3WJtSHIloRtG7OWrM_9KfRHcen6Wcw75rl5W55eYgkCQFxgDxngcDPBD-N8zz1w18DFNwSp_tfnOw3a3aF1Q</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Fernandes-Júnior, Paulo Ivan</creator><creator>Aidar, Saulo de Tarso</creator><creator>Morgante, Carolina Vianna</creator><creator>Gava, Carlos Alberto Tuão</creator><creator>Zilli, Jerri Édson</creator><creator>Souza, Layane Silva Barbosa de</creator><creator>Marinho, Rita de Cássia Nunes</creator><creator>Nóbrega, Rafaela Simão Abrahão</creator><creator>Brasil, Marivaine da Silva</creator><creator>Seido, Sirando Lima</creator><creator>Martins, Lindete Míria Vieira</creator><general>Sociedade Brasileira de Ciência do Solo</general><scope>AAYXX</scope><scope>CITATION</scope><scope>GPN</scope><scope>DOA</scope></search><sort><creationdate>20150801</creationdate><title>THE RESURRECTION PLANT TRIPOGON SPICATUS (POACEAE) HARBORS A DIVERSITY OF PLANT GROWTH PROMOTING BACTERIA IN NORTHEASTERN BRAZILIAN CAATINGA</title><author>Fernandes-Júnior, Paulo Ivan ; Aidar, Saulo de Tarso ; Morgante, Carolina Vianna ; Gava, Carlos Alberto Tuão ; Zilli, Jerri Édson ; Souza, Layane Silva Barbosa de ; Marinho, Rita de Cássia Nunes ; Nóbrega, Rafaela Simão Abrahão ; Brasil, Marivaine da Silva ; Seido, Sirando Lima ; Martins, Lindete Míria Vieira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-8f80ddfcb296b9fbf346270126a1ba70eaaa43165c3c5d380e0777a0721aa9813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>bactérias diazotróficas</topic><topic>fixação biológica do nitrogênio</topic><topic>inoculante</topic><topic>plantas tolerantes à dessecação</topic><topic>Semiárido</topic><topic>SOIL SCIENCE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandes-Júnior, Paulo Ivan</creatorcontrib><creatorcontrib>Aidar, Saulo de Tarso</creatorcontrib><creatorcontrib>Morgante, Carolina Vianna</creatorcontrib><creatorcontrib>Gava, Carlos Alberto Tuão</creatorcontrib><creatorcontrib>Zilli, Jerri Édson</creatorcontrib><creatorcontrib>Souza, Layane Silva Barbosa de</creatorcontrib><creatorcontrib>Marinho, Rita de Cássia Nunes</creatorcontrib><creatorcontrib>Nóbrega, Rafaela Simão Abrahão</creatorcontrib><creatorcontrib>Brasil, Marivaine da Silva</creatorcontrib><creatorcontrib>Seido, Sirando Lima</creatorcontrib><creatorcontrib>Martins, Lindete Míria Vieira</creatorcontrib><collection>CrossRef</collection><collection>SciELO</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Revista Brasileira de Ciência do Solo</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandes-Júnior, Paulo Ivan</au><au>Aidar, Saulo de Tarso</au><au>Morgante, Carolina Vianna</au><au>Gava, Carlos Alberto Tuão</au><au>Zilli, Jerri Édson</au><au>Souza, Layane Silva Barbosa de</au><au>Marinho, Rita de Cássia Nunes</au><au>Nóbrega, Rafaela Simão Abrahão</au><au>Brasil, Marivaine da Silva</au><au>Seido, Sirando Lima</au><au>Martins, Lindete Míria Vieira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE RESURRECTION PLANT TRIPOGON SPICATUS (POACEAE) HARBORS A DIVERSITY OF PLANT GROWTH PROMOTING BACTERIA IN NORTHEASTERN BRAZILIAN CAATINGA</atitle><jtitle>Revista Brasileira de Ciência do Solo</jtitle><addtitle>Rev. Bras. Ciênc. Solo</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>39</volume><issue>4</issue><spage>993</spage><epage>1002</epage><pages>993-1002</pages><issn>0100-0683</issn><issn>1806-9657</issn><eissn>0100-0683</eissn><abstract>Plant species that naturally occur in the Brazilian Caatinga(xeric shrubland) adapt in several ways to these harsh conditions, and that can be exploited to increase crop production. Among the strategic adaptations to confront low water availability, desiccation tolerance stands out. Up to now, the association of those species with beneficial soil microorganisms is not well understood. The aim of this study was to characterize Tripogon spicatusdiazotrophic bacterial isolates from the Caatingabiome and evaluate their ability to promote plant growth in rice. Sixteen bacterial isolates were studied in regard to their taxonomic position by partial sequencing of the 16S rRNA gene, putative diazotrophic capacity, in vitro indole-acetic acid (IAA) production and calcium phosphate solubilization, metabolism of nine different C sources in semi-solid media, tolerance to different concentrations of NaCl to pHs and intrinsic resistance to nine antibiotics. Finally, the ability of the bacterial isolates to promote plant growth was evaluated using rice (Oryza sativa) as a model plant. Among the 16 isolates evaluated, eight of them were classified as Enterobacteriaceae members, related to Enterobacter andPantoeagenera. Six other bacteria were related toBacillus, and the remaining two were related toRhizobiumand Stenotrophomonas.The evaluation of total N incorporation into the semi-solid medium indicated that all the bacteria studied have putative diazotrophic capacity. Two bacteria were able to produce more IAA than that observed for the strain BR 11175Tof Herbaspirillum seropedicae.Bacterial isolates were also able to form a microaerophilic pellicle in a semi-solid medium supplemented with different NaCl concentrations up to 1.27 mol L-1. Intrinsic resistance to antibiotics and the metabolism of different C sources indicated a great variation in physiological profile. Seven isolates were able to promote rice growth, and two bacteria were more efficient than the reference strainAzospirillum brasilense, Ab-V5. The results indicate the potential of T. spicatus as native plant source of plant growth promoting bacteria.
As espécies vegetais que ocorrem naturalmente na Caatinga brasileira apresentam diversas adaptações às condições adversas que podem ser exploradas para aumentar a produção vegetal. Entre essas estratégias adaptativas para o enfrentamento da baixa disponibilidade hídrica, a tolerância à dessecação pode ser destacada. Até o momento, a associação entre essas espécies e os microrganismos benéficos do solo não é bem entendida. Os objetivos deste trabalho foram caracterizar bactérias diazotróficas de Tripogon spicatus e avaliar a capacidade de promoção do crescimento delas em plantas de arroz. Dezesseis isolados bacterianos foram estudados quanto a seu posicionamento taxonômico por meio do sequenciamento parcial do gene 16S rRNA, potencial diazotrófico, produção de ácido indol acético (AIA) e solubilização de fosfato de cálcio in vitro, metabolismo de nove diferentes fontes de C em meio semissólido, tolerância a diferentes concentrações de NaCl a pHs e resistência intrínseca a nove antibióticos. A habilidade em promover o crescimento vegetal foi avaliada utilizando o arroz (Oryza sativa) como planta-modelo. Dentre os 16 isolados avaliados, oito foram classificados como pertencentes à família Enterobacteriaceae, relacionados aos gêneros Enterobacter e Pantoea. Seis outros isolados foram relacionados a Bacillus e outros dois a Rhizobium e Stenotrophomonas. A avaliação da incorporação do N ao meio semissólido demonstrou o potencial diazotrófico de todos os isolados. Duas bactérias produziram mais AIA do que a estirpe BR 11175T de Herbaspirillum seropedicae. Isolados bacterianos foram capazes de formar película em meio semissólido suplementado com concentrações de NaCl de até 1.27 mol L-1. A resistência intrínseca a antibióticos e o metabolismo de diferentes fontes de C indicaram um perfil fisiológico muito variável. Sete isolados foram capazes de aumentar o crescimento das plantas de arroz, mais eficientemente do que a estirpe de referência Ab-V5 de Azospirillum brasilense. Os resultados indicaram o potencial de T. spicatus como uma espécie nativa fonte de bactérias promotoras de crescimento vegetal.</abstract><pub>Sociedade Brasileira de Ciência do Solo</pub><doi>10.1590/01000683rbcs20140646</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0100-0683 |
| ispartof | Revista Brasileira de Ciência do Solo, 2015-08, Vol.39 (4), p.993-1002 |
| issn | 0100-0683 1806-9657 0100-0683 |
| language | eng |
| recordid | cdi_crossref_primary_10_1590_01000683rbcs20140646 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | bactérias diazotróficas fixação biológica do nitrogênio inoculante plantas tolerantes à dessecação Semiárido SOIL SCIENCE |
| title | THE RESURRECTION PLANT TRIPOGON SPICATUS (POACEAE) HARBORS A DIVERSITY OF PLANT GROWTH PROMOTING BACTERIA IN NORTHEASTERN BRAZILIAN CAATINGA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T18%3A02%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-scielo_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=THE%20RESURRECTION%20PLANT%20TRIPOGON%20SPICATUS%20(POACEAE)%20HARBORS%20A%20DIVERSITY%20OF%20PLANT%20GROWTH%20PROMOTING%20BACTERIA%20IN%20NORTHEASTERN%20BRAZILIAN%20CAATINGA&rft.jtitle=Revista%20Brasileira%20de%20Ci%C3%AAncia%20do%20Solo&rft.au=Fernandes-J%C3%BAnior,%20Paulo%20Ivan&rft.date=2015-08-01&rft.volume=39&rft.issue=4&rft.spage=993&rft.epage=1002&rft.pages=993-1002&rft.issn=0100-0683&rft.eissn=0100-0683&rft_id=info:doi/10.1590/01000683rbcs20140646&rft_dat=%3Cscielo_doaj_%3ES0100_06832015000400993%3C/scielo_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_scielo_id=S0100_06832015000400993&rft_doaj_id=oai_doaj_org_article_81f31a1224f74a3db4aad8b51cad87b7&rfr_iscdi=true |