Development and application of chromatographic methods for glycerolipid analysis
In the past 30 years, chiral-phase high-performance liquid chromatography (HPLC) has progressed from an application practiced by just a few chromatographers to a popular method widely used in many areas of pharmaceutical and biological sciences, including lipid chemistry. Since we demonstrated HPLC...
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| Veröffentlicht in: | CHROMATOGRAPHY 2011/08/20, Vol.32(2), pp.59-72 |
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| container_title | CHROMATOGRAPHY |
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| creator | Itabashi, Yutaka |
| description | In the past 30 years, chiral-phase high-performance liquid chromatography (HPLC) has progressed from an application practiced by just a few chromatographers to a popular method widely used in many areas of pharmaceutical and biological sciences, including lipid chemistry. Since we demonstrated HPLC resolution of mono- and diacylglycerol enantiomers on chiral stationary phases in the 1980s, the methodology has been extended to the resolution of various synthetic and naturally occurring chiral glycerolipids. Using reversed-phase HPLC, we also demonstrated the successful resolution of 1,2-diacylglycerol regioisomers (reverse isomers), which had remained a major unsolved problem in glycerolipid chromatography. These developments of the methodology permit further expansion of lipidomics and better understanding of lipid metabolism. |
| doi_str_mv | 10.15583/jpchrom.2011.005 |
| format | Article |
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| subjects | derivatization diastereomer enantiomer glycerolipid high-performance liquid chromatography mass spectrometry reverse isomer |
| title | Development and application of chromatographic methods for glycerolipid analysis |
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